Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	155	60.3	2039550	99.3%	2025273	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,734,017	T→C	S29G (AGC→GGC)	grxD ←	glutaredoxin 4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,734,017	0	T	C	100.0%	160.0 / NA	46	S29G (AGC→GGC)	grxD	glutaredoxin 4
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (21/25); total (21/25)

CGGGAAGGTCGGCCAGTTAGCATATTTCGGCAGTTCCGCACGAATGTCCGGATTCTGCAGAATATCAACATAGGCAAAACGTTCGCCACATGCGGCAAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCTCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTTCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTACGGCAGTAATCTGACATTGTAGCGGGT  >  NC_000913/1733886‑1734153

cGGGAAGGTCGGCCAGTTAGCATATTTCGGCAGTTCCGCACGAATGTCCGGATTCTGCAGAATATCAACATAGGCAAAACGTTCGCCACATGCGGCAAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCCCGGCAGtt                                                                                                                                  >  1:343909/1‑140 (MQ=255)

cGGGAAGGTCGGCCAGTTAGCATATTTCGGCAGTTCCGCACGAATGTCCGGATTCTGCAGAATATCAACATAGGCAAAACGTTCGCCACATGCGGCAAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCCCGGCAGtt                                                                                                                                  >  2:13027/1‑140 (MQ=255)

  ggAAGGTCGGCCAGTTAGCATATTTCGGCAGTTCCGCACGAATGTCCGGATTCTGCAGAATATCAACATAGGCAAAACGTTCGCCACATGCGGCAAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCCCGGCAGTTTc                                                                                                                                <  2:134815/140‑1 (MQ=255)

       gTCGGCCAGTTAGCATATTTCGGCAGTTCCGCACGAATGTCCGGATTCTGCAGAATATCAACATAGGCAAAACGTTCGCCACATGCGGCAAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCCCGGCAGTTTCGGTGa                                                                                                                           <  2:906565/140‑1 (MQ=255)

         cGGCCAGTTAGCATATTTCGGCAGTTCCGCACGAATGTCCGGATTCTGCAGAATATCAACATAGGCAAAACGTTCGCCACATGCGGCAAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCCCGGCAGTTTCGGTGAAc                                                                                                                         <  2:574393/140‑1 (MQ=255)

         cGGCCAGTTAGCATATTTCGGCAGTTCCGCACGAATGTCCGGATTCTGCAGAATATCAACATAGGCAAAACGTTCGCCACATGCGGCAAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCCCGGCAGTTTCGGTGAAc                                                                                                                         <  1:363212/140‑1 (MQ=255)

         cGGCCAGTTAGCATATTTCGGCAGTTCCGCACGAATGTCCGGATTCTGCAGAATATCAACATAGGCAAAACGTTCGCCACATGCGGCAAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCCCGGCAGTTTCGGTGAAc                                                                                                                         <  1:989152/140‑1 (MQ=255)

                ttAGCATATTTCGGCAGTTCCGCACGAATGTCCGGATTCTGCAGAATATCAACATAGGCAAAACGTTCGCCACATGCGGCAAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCCCGGCAGTTTCGGTGAAc                                                                                                                         >  2:372787/1‑133 (MQ=255)

                ttAGCATATTTCGGCAGTTCCGCACGAATGTCCGGATTCTGCAGAATATCAACATAGGCAAAACGTTCGCCACATGCGGCAAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCCCGGCAGTTTCGGTGAAc                                                                                                                         <  1:372787/133‑1 (MQ=255)

                          tCGGCAGTTCCGCACGAATGTCCGGATTCTGCAGAATATCAACATAGGCAAAACGTTCGCCACATGCGGCAAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCCCGGCAGTTTCGGTGAACCTTTCATGTACAGCAgg                                                                                                        >  2:714773/1‑140 (MQ=255)

                              cAGTTCCGCACGAATGTCCGGATTCTGCAGAATATCAACATAGGCAAAACGTTCGCCACATGCGGCAAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCCCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCg                                                                                                    >  2:888086/1‑140 (MQ=255)

                                      cACGAATGTCCGGATTCTGCAGAATATCAACATAGGCAAAACGTTCGCCACATGCGGCAAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCCCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCa                                                                                            >  1:197062/1‑140 (MQ=255)

                                      cACGAATGTCCGGATTCTGCAGAATATCAACATAGGCAAAACGTTCGCCACATGCGGCAAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCCCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCa                                                                                            >  1:485585/1‑140 (MQ=255)

                                           aTGTCCGGATTCTGCAGAATATCAACATAGGCAAAACGCTCGCCACATGCGGCAAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCCCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAAt                                                                                       >  1:648858/1‑140 (MQ=255)

                                                   aTTCTGCAGAATATCAACATAGGCAAAACGTTCGCCACATGCGGCAAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCCCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGtt                                                                               <  1:13027/140‑1 (MQ=255)

                                                   aTTCTGCAGAATATCAACATAGGCAAAACGTTCGCCACATGCGGCAAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCCCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGtt                                                                               >  2:226455/1‑140 (MQ=255)

                                                                   aCATAGGCAAAACGTTCGCCACATGCGGCAAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCCCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTTCGATAgt                                                               >  2:858187/1‑140 (MQ=255)

                                                                   aCATAGGCAAAACGTTCGCCACATGCGGCAAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCCCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTTCGATAgt                                                               <  1:888086/140‑1 (MQ=255)

                                                                    cATAGGCAAAACGTTCGCCACATGCGGCAAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCCCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTTCGATAgtg                                                              <  2:990479/140‑1 (MQ=255)

                                                                     aTAGGCAAAACGTTCGCCACATGCGGCAAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCCCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTTCGATAgtgg                                                             <  1:226455/140‑1 (MQ=255)

                                                                      tAGGCAAAACGTTCGCCACATGCGGCAAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCCCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTTCGa                                                                   <  1:517259/133‑1 (MQ=255)

                                                                      tAGGCAAAACGTTCGCCACATGCGGCAAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCCCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTTCGa                                                                   >  2:517259/1‑133 (MQ=255)

                                                                      tAGGCAAAACGTTCGCCACATGCGGCAAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCCCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTTCGATa                                                                 >  2:301763/1‑135 (MQ=255)

                                                                      tAGGCAAAACGTTCGCCACATGCGGCAAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCCCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTTCGATa                                                                 <  1:301763/135‑1 (MQ=255)

                                                                      tAGGCAAAACGTTCGCCACATGCGGCAAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCCCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTTCGATAgtggt                                                            <  2:971215/140‑1 (MQ=255)

                                                                        ggCAAAACGTTCGCCACATGCGGCAAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCCCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTTCGATAGTGGTGc                                                          >  1:55541/1‑140 (MQ=255)

                                                                         gCAAAACGTTCGCCACATGCGGCAAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCCCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTTCGATAGTGGTGCt                                                         <  2:984266/140‑1 (MQ=255)

                                                                         gCAAAACGTTCGCCACATGCGGCAAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCCCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTTCGATAGTGGTGCt                                                         >  1:236786/1‑140 (MQ=255)

                                                                                  tCGCCACATGCGGCAAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCCCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTTCGATAGTGGTGCTCATTGTCtt                                                >  1:807277/1‑140 (MQ=255)

                                                                                  tCGCCACATGCGGCAAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCCCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTTCGATAGTGGTGCTCATTGTCtt                                                >  2:433712/1‑140 (MQ=255)

                                                                                           gCGGCAAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCCCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTTCGATAGTGGTGCTCATTGTCTTGCTTCCTCa                                       <  2:1000479/140‑1 (MQ=255)

                                                                                                     ccTGGACTGCCTGGGCAGAGAAACCGCAGCCCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTTCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTa                             <  2:197062/140‑1 (MQ=255)

                                                                                                         gACTGCCTGGGCAGAGAAACCGCAGCCCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTTCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTACGGc                         <  1:714773/140‑1 (MQ=255)

                                                                                                            tGCCTGGGCAGAGAAACCGCAGCCCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTTCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTACGGCAGt                      <  2:404517/140‑1 (MQ=255)

                                                                                                              ccTGGGCAGAGAAACCGCAGCCCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTTCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTa                             <  2:132851/131‑1 (MQ=255)

                                                                                                              ccTGGGCAGAGAAACCGCAGCCCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTTCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTa                             >  1:132851/1‑131 (MQ=255)

                                                                                                              ccTGGGCAGAGAAACCGCAGCCCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTTCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTACGGCAGTaa                    >  2:690642/1‑140 (MQ=255)

                                                                                                              ccTGGGCAGAGAAACCGCAGCCCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTTCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTACGGCAGTaa                    >  1:525334/1‑140 (MQ=255)

                                                                                                              ccTGGGCAGAGAAACCGCAGCCCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTTCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTACGGCAGTaa                    >  1:108505/1‑140 (MQ=255)

                                                                                                                tGGGCAGAGAAACCGCAGCCCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTTCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTACGGCAGTAATc                  <  2:343909/140‑1 (MQ=255)

                                                                                                                 gggCAGAGAAACCGCAGCCCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTTCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTACGGCAGTAATCt                 <  1:593804/140‑1 (MQ=255)

                                                                                                                    cAGAGAAACCGCAGCCCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTTCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTACGGCAGTAATCTGAc              >  1:703485/1‑140 (MQ=255)

                                                                                                                           aCCGCAGCCCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTTCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTACGGCAGTAATCTGACATTGTAg       <  2:525334/140‑1 (MQ=255)

                                                                                                                                aGCCCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTTCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTACGGCAGTAATCTGACATTGTAGCGGGt  <  2:485585/140‑1 (MQ=255)

                                                                                                                                aGCCCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTTCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTACGGCAGTAATCTGACATTGTAGCGGGt  <  2:55541/140‑1 (MQ=255)

                                                                                                                                aGCCCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTTCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTACGGCAGTAATCTGACATTGTAGCGGGt  <  1:858187/140‑1 (MQ=255)

CGGGAAGGTCGGCCAGTTAGCATATTTCGGCAGTTCCGCACGAATGTCCGGATTCTGCAGAATATCAACATAGGCAAAACGTTCGCCACATGCGGCAAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCTCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTTCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTACGGCAGTAATCTGACATTGTAGCGGGT  >  NC_000913/1733886‑1734153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A