Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	155	60.3	2039550	99.3%	2025273	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,757,584	T→C	V55A (GTT→GCT)	lpp →	murein lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,757,584	0	T	C	100.0%	155.4 / NA	44	V55A (GTT→GCT)	lpp	murein lipoprotein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (19/25); total (19/25)

TCCTGGGTTCTACTCTGCTGGCAGGTTGCTCCAGCAACGCTAAAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGC  >  NC_000913/1757455‑1757711

tCCTGGGTTCTACTCTGCTGGCAGGTTGCTCCAGCAACGCTAAAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGCTCAGgctgct                                                                                                                       <  2:770581/140‑1 (MQ=255)

     ggTTCTACTCTGCTGGCAGGTTGCTCCAGCAACGCTAAAATTGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGCTCAGGCTGCTAAAGa                                                                                                                  >  1:418841/1‑140 (MQ=255)

         cTACTCTGCTGGCAGGTTGCTCCAGCAACGCTAAAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGCTCAGGCTGCTAAAGATGAc                                                                                                              <  1:249102/140‑1 (MQ=255)

          tACTCTGCTGGCAGGTTGCTCCAGCAACGCTAAAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGCTCAGGCTGCTaaa                                                                                                                    <  1:448104/133‑1 (MQ=255)

          tACTCTGCTGGCAGGTTGCTCCAGCAACGCTAAAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGCTCAGGCTGCTaaa                                                                                                                    >  2:448104/1‑133 (MQ=255)

          tACTCTGCTGGCAGGTTGCTCCAGCAACGCTAAAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGCTCAGGCTGCTAAAGATGACg                                                                                                             >  1:952941/1‑140 (MQ=255)

          tACTCTGCTGGCAGGTTGCTCCAGCAACGCTAAAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGCTCAGGCTGCTAAAGATGACg                                                                                                             >  1:916928/1‑140 (MQ=255)

              ctgctgGCAGGTTGCTCCAGCAACGCTAAAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGCTCAGGCTGCTAAAGATGACGCAGc                                                                                                         <  2:952941/140‑1 (MQ=255)

              ctgctgGCAGGTTGCTCCAGCAACGCTAAAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGCTCAGGCTGCTAAAGATGACGCAGc                                                                                                         <  1:892823/140‑1 (MQ=255)

                gctgGCAGGTTGCTCCAGCAACGCTAAAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGCTCAGGCTGCTAAAGATGACGCAGCTc                                                                                                       <  2:927527/140‑1 (MQ=255)

                 ctgGCAGGTTGCTCCAGCAACGCTAAAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGCTCAGGCTGCTAAAGATGACGCAGCTCg                                                                                                      >  1:959460/1‑140 (MQ=255)

                  tgGCAGGTTGCTCCAGCAACGCTAAAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGCTCAGGCTGCTAAAGATGACGCAGCTCGt                                                                                                     <  2:465450/140‑1 (MQ=255)

                         ttGCTCCAGCAACGCTAAAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGCTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAAcc                                                                                              <  2:241600/140‑1 (MQ=255)

                          tGCTCCAGCAACGCTAAAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGCTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCa                                                                                             <  2:959460/140‑1 (MQ=255)

                            cTCCAGCAACGCTAAAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGCTCAGGCTGCTAAAGATGACGCAGCTCGTGc                                                                                                   <  2:260714/132‑1 (MQ=255)

                            cTCCAGCAACGCTAAAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGCTCAGGCTGCTAAAGATGACGCAGCTCGTGc                                                                                                   >  1:260714/1‑132 (MQ=255)

                            cTCCAGCAACGCTAAAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGCTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGc                                                                                           <  1:423226/140‑1 (MQ=255)

                              ccAGCAACGCTAAAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGCTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCGGCGt                                                                                         <  2:206855/140‑1 (MQ=255)

                                aGCAACGCTAAAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCGGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGCTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCt                                                                                       >  2:909739/1‑140 (MQ=255)

                                      gCTAAAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGCTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGacaa                                                                                 <  1:819186/140‑1 (MQ=255)

                                           aaTCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGCTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATgg                                                                            <  2:995069/140‑1 (MQ=255)

                                           aaTCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGCTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATgg                                                                            <  2:553807/140‑1 (MQ=255)

                                           aaTCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGCTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATgg                                                                            <  1:909739/140‑1 (MQ=255)

                                           aaTCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGCTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATgg                                                                            <  1:178776/140‑1 (MQ=255)

                                                aTCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGCTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGac                                                                                   >  2:463198/1‑128 (MQ=255)

                                                aTCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGCTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGac                                                                                   <  1:463198/128‑1 (MQ=255)

                                                       gTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGCTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATAcc                                                                <  2:93120/140‑1 (MQ=255)

                                                         cttctGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGCTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGc                                                              <  2:701327/140‑1 (MQ=255)

                                                               aCGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGCTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTaa                                                        <  1:536910/140‑1 (MQ=255)

                                                                  ttCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGCTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAg                                                     >  1:964015/1‑140 (MQ=255)

                                                                  ttCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGCTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAg                                                     >  2:165347/1‑140 (MQ=255)

                                                                       aCTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGCTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCaa                                                            >  1:705594/1‑128 (MQ=255)

                                                                       aCTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGCTCAGGCTGCCAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCaa                                                            <  2:705594/128‑1 (MQ=255)

                                                                           tGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGCTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTgtga                                            >  1:10301/1‑140 (MQ=255)

                                                                            gAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGCTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTgtgaa                                           <  2:733064/140‑1 (MQ=255)

                                                                                  tAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGCTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGaaa                                     >  2:345233/1‑140 (MQ=255)

                                                                                       ttGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGCTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATgg                                >  1:546146/1‑140 (MQ=255)

                                                                                              gCTGAGCAACGACGTGAACGCAATGCGTTCCGACGCTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACAt                         >  1:229917/1‑140 (MQ=255)

                                                                                                  aGCAACGACGTGAACGCAATGCGTTCCGACGCTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATtgtg                     <  2:229917/140‑1 (MQ=255)

                                                                                                       cgacgTGAACGCAATGCGTTCCGACGCTAAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCa                >  2:361760/1‑140 (MQ=255)

                                                                                                         acgTGAACGCAATGCGTTCCGACGCTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCAtt              >  2:999933/1‑140 (MQ=255)

                                                                                                              aaCGCAATGCGTTCCGACGCTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCAttttttt         >  1:17774/1‑140 (MQ=255)

                                                                                                                    aTGCGTTCCGACGCTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTg   <  1:999933/140‑1 (MQ=255)

                                                                                                                     tGCGTTCCGACGCTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGc  >  2:157042/1‑140 (MQ=255)

TCCTGGGTTCTACTCTGCTGGCAGGTTGCTCCAGCAACGCTAAAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGC  >  NC_000913/1757455‑1757711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A