Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	183	98.6	3427438	96.9%	3321187	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,763,454	T→C	T186A (ACC→GCC)	sufB ←	Fe‑S cluster scaffold complex subunit SufB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,763,454	0	T	C	100.0%	181.8 / NA	50	T186A (ACC→GCC)	sufB	Fe‑S cluster scaffold complex subunit SufB
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (21/29); total (21/29)

TTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCC  >  NC_000913/1763318‑1763590

ttttCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGCGcc                                                                                                                                       <  2:1030704/140‑1 (MQ=255)

ttttCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGCGcc                                                                                                                                       <  1:795431/140‑1 (MQ=255)

  ttCTGCGTTAATGCGAAAATAGGAGGAAAGTTCCATCGGGCAGCGCATGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGCGCCga                                                                                                                                     <  2:1381480/140‑1 (MQ=255)

    cTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGCGCCgaga                                                                                                                                   <  2:1411746/140‑1 (MQ=255)

     tGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGCGCCGAGat                                                                                                                                  <  2:1300942/140‑1 (MQ=255)

          tAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGCGCCGAGATATTTa                                                                                                                             <  2:461399/140‑1 (MQ=255)

            aTGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGCGCCGAGATATTTACg                                                                                                                           >  1:1577521/1‑140 (MQ=255)

              gCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGCGCCGAGATATTTACGCa                                                                                                                         <  1:1670932/140‑1 (MQ=255)

               cGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGCGCCGAGATATTTACGCAc                                                                                                                        >  2:989353/1‑140 (MQ=255)

                gAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGCGCCGAGATATTTACGCAcc                                                                                                                       <  1:1114989/140‑1 (MQ=255)

                 aaaaTAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGCGCCGAGATATTTACGCACCa                                                                                                                      >  2:456242/1‑140 (MQ=255)

                         tGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGCGCCGAGATATTTACGCACCAGTTCCggg                                                                                                              <  1:1140324/140‑1 (MQ=255)

                         tGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGCGCCGAGATATTTACGCACCAGTTCCggg                                                                                                              <  1:1120398/140‑1 (MQ=255)

                         tGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGCGCCGAGATATTTACGCACCAGTTCCggg                                                                                                              <  2:1577521/140‑1 (MQ=255)

                         tGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGCGCCGAGATATTTACGCACCAGTTCCggg                                                                                                              <  2:1582031/140‑1 (MQ=255)

                         gggAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGCGCCGAGATATTTACGCACCAGTTCCggg                                                                                                              <  2:53014/139‑1 (MQ=255)

                           gAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGCGCCGAGATATTTACGCACCAGTTCCggg                                                                                                              <  2:1233498/138‑1 (MQ=255)

                           gAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGCGCCGAGATATTTACGCACCAGTTCCggg                                                                                                              >  1:1233498/1‑138 (MQ=255)

                                ttCCATTGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGCGCCGAGATATTTACGCACCAGTTCCGGGTGATCGt                                                                                                       >  1:813507/1‑140 (MQ=255)

                                       gggCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGCGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGc                                                                                                <  1:1711971/140‑1 (MQ=255)

                                          cAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGCGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTc                                                                                             >  2:318858/1‑140 (MQ=255)

                                                           cacaTAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGCGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGaaaa                                                                            <  1:1262972/140‑1 (MQ=255)

                                                            acaTAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGCGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAaat                                                                           >  1:784239/1‑140 (MQ=255)

                                                             caTAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGCGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAaata                                                                          <  1:318858/140‑1 (MQ=255)

                                                              ataaataaACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGCGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAaataa                                                                         >  2:1123018/1‑140 (MQ=255)

                                                              ataaataaACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGCGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAaataa                                                                         >  2:1411590/1‑140 (MQ=255)

                                                              ataaataaACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGCGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAaataa                                                                         >  1:1460270/1‑140 (MQ=255)

                                                                          aCCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGCGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCg                                                             >  1:264738/1‑140 (MQ=255)

                                                                          aCCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGCGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCg                                                             >  1:1560081/1‑140 (MQ=255)

                                                                            cATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGCGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGcc                                                           >  1:780673/1‑140 (MQ=255)

                                                                                       aCCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGCGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCCCCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTcgcg                                                <  1:1093010/140‑1 (MQ=255)

                                                                                         cgccgcATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGCGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGAt                                              >  1:698988/1‑140 (MQ=255)

                                                                                              cATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGCGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGAt                                              >  1:1208079/1‑135 (MQ=255)

                                                                                              cATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGCGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGAt                                              <  2:1208079/135‑1 (MQ=255)

                                                                                              cATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGCGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAagta                                         >  1:1142467/1‑140 (MQ=255)

                                                                                                  aaGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGCGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTgg                                     <  2:1560081/140‑1 (MQ=255)

                                                                                                   aGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGCGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGc                                    <  1:989353/140‑1 (MQ=255)

                                                                                                   aGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGCGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGc                                    <  1:720824/140‑1 (MQ=255)

                                                                                                   aGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGCGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGc                                    <  1:1592432/140‑1 (MQ=255)

                                                                                                         gCAAAGAAGTTGTCATTCCCCGGCACCACGGCGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAagta                                         >  1:793424/1‑129 (MQ=255)

                                                                                                         gCAAAGAAGTTGTCATTCCCCGGCACCACGGCGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAagta                                         <  2:793424/129‑1 (MQ=255)

                                                                                                           aaaGAAGTTGTCATTCCCCGGCACCACGGCGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGaaa                            <  1:1123018/140‑1 (MQ=255)

                                                                                                                 gTTGTCATTCCCCGGCACCACGGCGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGt                      <  1:574730/140‑1 (MQ=255)

                                                                                                                  ttGTCATTCCCCGGCACCACGGCGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTc                     >  2:1683366/1‑140 (MQ=255)

                                                                                                                            ccGGCACCACGGCGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCa           >  1:1267957/1‑140 (MQ=255)

                                                                                                                             cGGCACCACGGCGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCAt          <  1:1531744/140‑1 (MQ=255)

                                                                                                                               gCACCACGGCGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATcc        >  1:791401/1‑140 (MQ=255)

                                                                                                                                caccacGGCGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCa       >  2:321885/1‑140 (MQ=255)

                                                                                                                                    acGGCGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGc   <  1:1676099/140‑1 (MQ=255)

                                                                                                                                     cGGCGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGcc  <  2:759116/140‑1 (MQ=255)

TTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCC  >  NC_000913/1763318‑1763590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A