Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	183	98.6	3427438	96.9%	3321187	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,765,157	T→C	intergenic (‑35/+65)	ydiH ← / ← menI	protein YdiH/1,4‑dihydroxy‑2‑naphthoyl‑CoA hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,765,157	0	T	C	100.0%	167.9 / NA	46	intergenic (‑35/+65)	ydiH/menI	protein YdiH/1,4‑dihydroxy‑2‑naphthoyl‑CoA hydrolase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (22/24); total (22/24)

TTCAGACGTTTGAGATATTGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCT  >  NC_000913/1765028‑1765289

ttCAGACGTTTGAGATATTGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTACACCACCGttt                                                                                                                            <  2:1509333/140‑1 (MQ=255)

 tCAGACGTTTGAGATATTGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTACACCACCGTTTa                                                                                                                           <  2:757413/140‑1 (MQ=255)

 tCAGACGTTTGAGATATTGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTACACCACCGTTTa                                                                                                                           <  2:533662/140‑1 (MQ=255)

 tCAGACGTTTGAGATATTGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTACACCACCGTTTa                                                                                                                           <  2:1220443/140‑1 (MQ=255)

 tCAGACGTTTGAGATACTGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTACACCACCGTTTa                                                                                                                           <  2:1534141/140‑1 (MQ=255)

  cAGACGTTTGAGATATTGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTACACCACCGTTTAc                                                                                                                          <  1:1668319/140‑1 (MQ=255)

   aGACGTTTGAGATATTGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTACACCACCGTTTACt                                                                                                                         >  2:1596998/1‑140 (MQ=255)

    gACGTTTGAGATATTGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAATATTCAATGGCCAGCTGGGTGGGATCAATTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTACACCACCGTTTACtg                                                                                                                        <  2:1530874/140‑1 (MQ=255)

      cGTTTGAGATATTGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTACACCACCGTTTACtgtg                                                                                                                      <  1:1343527/140‑1 (MQ=255)

       gTTTGAGATATTGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTACACCACCGTTTACTGTGc                                                                                                                     <  1:582014/140‑1 (MQ=255)

                 ttGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGAGATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTACACCACCGTTTACTGTGCAGATAATGAc                                                                                                           <  2:689655/140‑1 (MQ=255)

                 ttGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTACACCACCGTTTACTGTGCAGATAATGAc                                                                                                           >  1:462093/1‑140 (MQ=255)

                  tGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTACACCACCGTTTACTGTGCAGATAATGAcc                                                                                                          <  1:1596998/140‑1 (MQ=255)

                   gggCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTACACCACCGTTTACTGTGCAGATAATGACCa                                                                                                         >  1:959075/1‑140 (MQ=255)

                    ggCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTACACCACCGTTTACTGTGCAGATAATGACCaa                                                                                                        >  1:1606997/1‑140 (MQ=255)

                       aGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTACACCACCGTTTACTGTGCAGATAATGACCAAAAg                                                                                                     >  2:1471994/1‑140 (MQ=255)

                                    ctctGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTACACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTcac                                                                                        >  2:1628035/1‑140 (MQ=255)

                                          tCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTACACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACtttt                                                                                  <  1:325756/140‑1 (MQ=255)

                                          tCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTACACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACtttt                                                                                  <  1:1471994/140‑1 (MQ=255)

                                               gCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTACACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGt                                                                             <  1:288052/140‑1 (MQ=255)

                                                   aaaaaTTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTACACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGCCACACTTTTCTGGTGACa                                                                         <  1:92328/140‑1 (MQ=255)

                                                          cAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTACACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTcac                                                                  >  1:1586956/1‑140 (MQ=255)

                                                                 cAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTACACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATgg                                                           <  2:1586956/140‑1 (MQ=255)

                                                                           ggATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTACACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCaa                                                 <  1:75436/140‑1 (MQ=255)

                                                                                 aGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTACACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGa                                           >  2:287917/1‑140 (MQ=255)

                                                                                   ttGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTACACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACg                                         <  1:1435160/140‑1 (MQ=255)

                                                                                      aGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTACACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAAc                                      >  1:1476946/1‑140 (MQ=255)

                                                                                       gTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTACACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACa                                     >  2:961833/1‑140 (MQ=255)

                                                                                           aCATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTACACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGcac                                 >  2:1376438/1‑140 (MQ=255)

                                                                                            cATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTACACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGcaca                                >  1:127109/1‑140 (MQ=255)

                                                                                                gCATCCTCGCTTTTAGACAAGACCTGCACAGTACACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACg                            >  1:1153568/1‑140 (MQ=255)

                                                                                                    ccTCGCTTTTAGACAAGACCTGCACAGTACACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCt                        >  1:702387/1‑140 (MQ=255)

                                                                                                    ccTCGCTTTTAGACAAGACCTGCACAGTACACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGCGACg                                         >  2:1297513/1‑123 (MQ=255)

                                                                                                    ccTCGCTTTTAGACAAGACCTGCACAGTACACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGCGACg                                         <  1:1297513/123‑1 (MQ=255)

                                                                                                     cTCGCTTTTAGACAAGACCTGCACAGTACACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAg                                    >  1:708773/1‑127 (MQ=255)

                                                                                                     cTCGCTTTTAGACAAGACCTGCACAGTACACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAg                                    <  2:708773/127‑1 (MQ=255)

                                                                                                     cTCGCTTTTAGACAAGACCTGCACAGTACACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGcaca                                >  1:501839/1‑131 (MQ=255)

                                                                                                     cTCGCTTTTAGACAAGACCTGCACAGTACACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGcaca                                <  2:501839/131‑1 (MQ=255)

                                                                                                     cTCGCTTTTAGACAAGACCTGCACAGTACACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCtt                       <  2:702387/140‑1 (MQ=255)

                                                                                                               gACAAGACCTGCACAGTACACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGaa             >  2:1073659/1‑140 (MQ=255)

                                                                                                                  aaGACCTGCACAGTACACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGAt          >  1:1016868/1‑140 (MQ=255)

                                                                                                                    gACCTGCACAGTACACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCa                  <  1:1539298/130‑1 (MQ=255)

                                                                                                                    gACCTGCACAGTACACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCa                  >  2:1539298/1‑130 (MQ=255)

                                                                                                                    gACCTGCACAGTACACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGAttt        >  1:1057940/1‑140 (MQ=255)

                                                                                                                       cTGCACAGTACACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCaa     <  2:1476946/140‑1 (MQ=255)

                                                                                                                          cacaGTACACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCt  >  2:621064/1‑140 (MQ=255)

TTCAGACGTTTGAGATATTGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCT  >  NC_000913/1765028‑1765289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A