Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,765,577	T→C	N19S (AAC→AGC)	menI ←	1,4‑dihydroxy‑2‑naphthoyl‑CoA hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,765,577	0	T	C	100.0%	185.0 / NA	52	N19S (AAC→AGC)	menI	1,4‑dihydroxy‑2‑naphthoyl‑CoA hydrolase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (23/29); total (23/29)

CTTTCGGCCAGTACCACGGATGCTCCTCCATGCAGCAACCCGAAAGGCTGCTTTGTCCGCGAGTCTACTGGCATTGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGTTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGTACCCCCGTGCCTTCAACCCGTTTTA  >  NC_000913/1765442‑1765693

cTTTCGGCCAGTACCACGGATGCTCCTCCATGCAGCAACCCGAAAGGCTGCTTTGTCCGCGAGTCTACTGGCATTGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGCTTcc                                                                                                                  <  2:843420/140‑1 (MQ=255)

  ttCGGCCAGTACCACGGATGCTCCTCCATGCAGCAACCCGAAAGGCTGCTTTGTCCGCGAGTCTACTGGCATTGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCtt                                                                                                                <  2:416310/140‑1 (MQ=255)

     ggCCAGTACCACGGATGCTCCTCCATGCAGCAACCCGAAAGGCTGCTTTGTCCGCGAGTCTACTGGCATTGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCAc                                                                                                             <  1:698213/140‑1 (MQ=255)

     ggCCAGTACCACGGATGCTCCTCCATGCAGCAACCCGAAAGGCTGCTTTGTCCGCGAGTCTACTGGCATTGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCAc                                                                                                             <  1:222778/140‑1 (MQ=255)

           tACCACGGATGCTCCTCCATGCAGCAACCCGAAAGGCTGCTTTGTCCGCGAGTCTACTGGCATTGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAg                                                                                                       >  1:432506/1‑140 (MQ=255)

               aCGGATGCTCCTCCATGCAGCAACCCGAAAGGCTGCTTTGTCCGCGAGTCTACTGGCATTGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAGCAtt                                                                                                   <  2:432506/140‑1 (MQ=255)

                cGGATGCTCCTCCATGCAGCAACCCGAAAGGCTGCTTTGTCCGCGAGTCTACTGGCATTGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAGCATTc                                                                                                  <  2:604627/140‑1 (MQ=255)

                    tGCTCCTCCATGCAGCAACCCGAAAAGCTGCTTTGTCCGCGAGTCTACTGGCATTGTCGCTTCAACGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAGCATTCAgcc                                                                                              >  2:718224/1‑138 (MQ=255)

                     gCTCCTCCATGCAGCAACCCGAAAGGCTGCTTTGTCCGCGAGTCTACTGGCATTGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAGCATTCAg                                                                                                <  2:740603/137‑1 (MQ=255)

                     gCTCCTCCATGCAGCAACCCGAAAGGCTGCTTTGTCCGCGAGTCTACTGGCATTGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAGCATTCAg                                                                                                >  1:740603/1‑137 (MQ=255)

                     gCTCCTCCATGCAGCAACCCGAAAGGCTGCTTTGTCCGCGAGTCTACTGGCATTGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAGCATTCAGTGc                                                                                             >  2:2572/1‑140 (MQ=255)

                     gCTCCTCCATGCAGCAACCCGAAAGGCTGCTTTGTCCGCGAGTCTACTGGCATTGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAGCATTCAGTGc                                                                                             >  1:525302/1‑140 (MQ=255)

                          tccATGCAGCAACCCGAAAGGCTGCTTTGTCCGCGAGTCTACTGGCATTGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAGCATTCAGTGCTTCCa                                                                                        <  1:45203/140‑1 (MQ=255)

                            cATGCAGCAACCCGAAAGGCTGCTTTGTCCGCGAGTCTACTGGCATTGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAGCATTCAGTGCTTCCAgg                                                                                      >  2:235054/1‑140 (MQ=255)

                                 agcaACCCGAAAGGCTGCTTTGTCCGCGAGTCTACTGGCATTGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGAt                                                                                 >  2:815842/1‑140 (MQ=255)

                                 agcaACCCGAAAGGCTGCTTTGTCCGCGAGTCTACTGGCATTGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGAt                                                                                 >  2:205932/1‑140 (MQ=255)

                                 agcaACCCGAAAGGCTGCTTTGTCCGCGAGTCTACTGGCATTGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGAt                                                                                 >  2:158498/1‑140 (MQ=255)

                                   caACCCGAAAGGCTGCTTTGTCCGCGAGTCTACTGGCATTGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGAttt                                                                               >  2:631380/1‑140 (MQ=255)

                                     aCCCGAAAGGCTGCTTTGTCCGCGAGTCTACTGGCATTGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTc                                                                             >  1:375540/1‑140 (MQ=255)

                                      cccGAAAGGCTGCTTTGTCCGCGAGTCTTCTGGCATTGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTcc                                                                            <  1:371561/140‑1 (MQ=255)

                                      cccGAAAGGCTGCTTTGTCCGCGAGTCTACTGGCATTGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTcc                                                                            <  2:553515/140‑1 (MQ=255)

                                           aaGGCTGCTTTGTCCGCGAGTCTACTGGCATTGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTc                                                                       >  1:181026/1‑140 (MQ=255)

                                                 gctTTGTCCGCGAGTCTACTGGCATTGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATc                                                                 >  1:572528/1‑140 (MQ=255)

                                                 gctTTGTCCGCGAGTCTACTGGCATTGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATc                                                                 <  1:815842/140‑1 (MQ=255)

                                                    ttGTCCGCGAGTCTACTGGCATTGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCAtt                                                              >  1:710051/1‑140 (MQ=255)

                                                    ttGTCCGCGAGTCTACTGGCATTGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCAtt                                                              <  2:525302/140‑1 (MQ=255)

                                                        ccGCGAGTCTACTGGCATTGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTtaat                                                          >  2:781063/1‑140 (MQ=255)

                                                        ccGCGAGTCTACTGGCATTGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTtaat                                                          >  2:293346/1‑140 (MQ=255)

                                                           cgAGTCTACTGGCATTGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTaataat                                                       <  1:158498/140‑1 (MQ=255)

                                                             aGTCTACTGGCATTGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAAtct                                                     <  1:249095/140‑1 (MQ=255)

                                                                       cATTGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGc                                           <  2:181026/140‑1 (MQ=255)

                                                                       cATTGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGc                                           <  1:205932/140‑1 (MQ=255)

                                                                       cATTGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGc                                           <  2:507671/140‑1 (MQ=255)

                                                                         ttGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCt                                         >  1:868138/1‑140 (MQ=255)

                                                                          tGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAAtc                                                      <  2:316137/126‑1 (MQ=255)

                                                                          tGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAAtc                                                      >  1:316137/1‑126 (MQ=255)

                                                                          tGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTg                                        >  1:425656/1‑140 (MQ=255)

                                                                           gTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTg                                        >  2:290896/1‑139 (MQ=255)

                                                                           gTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTg                                        <  1:290896/139‑1 (MQ=255)

                                                                            tCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGca                                      <  2:375540/140‑1 (MQ=255)

                                                                              gCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGcaca                                    >  1:358560/1‑140 (MQ=255)

                                                                                 tCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGa                                 <  1:394369/140‑1 (MQ=255)

                                                                                 tCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGa                                 <  1:2572/140‑1 (MQ=255)

                                                                                      ggTGTCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCg                            <  1:235054/140‑1 (MQ=255)

                                                                                        tgtCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGTa                          <  1:781063/140‑1 (MQ=255)

                                                                                          tcatcaCCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGTAcc                        <  1:293346/140‑1 (MQ=255)

                                                                                                ccAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGa                                 <  1:426546/125‑1 (MQ=255)

                                                                                                ccAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGa                                 >  2:426546/1‑125 (MQ=255)

                                                                                                ccAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGTACCCCCGTg                  >  1:377706/1‑140 (MQ=255)

                                                                                                       tttCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGTTCCCCCGTGCCTTCaa           <  2:322681/139‑1 (MQ=255)

                                                                                                       gTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTTCACAGGATGGCGTACCCCCGTGCCTTCaa           <  2:679373/140‑1 (MQ=255)

                                                                                                       gTTCAAAGCGAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGTACCCCCGTGCCTTCaa           <  2:412419/140‑1 (MQ=255)

                                                                                                           aaaGCGAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGTACCCCCGTGCCTTCAACCCg       >  1:276753/1‑140 (MQ=255)

                                                                                                                gAATATCCAGGAACCCCACCATGCTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGTACCCCCGTGCCTTCAACCCGTTTTa  <  2:425656/140‑1 (MQ=255)

CTTTCGGCCAGTACCACGGATGCTCCTCCATGCAGCAACCCGAAAGGCTGCTTTGTCCGCGAGTCTACTGGCATTGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGTTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGTACCCCCGTGCCTTCAACCCGTTTTA  >  NC_000913/1765442‑1765693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A