Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	155	60.3	2039550	99.3%	2025273	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,809,183	A→G	G17G (GGT→GGC)	yniB ←	uncharacterized protein YniB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,809,183	0	A	G	100.0%	190.1 / NA	54	G17G (GGT→GGC)	yniB	uncharacterized protein YniB
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (29/25); total (29/25)

CTGCATCATATCGATCATGACATGAGTAAAGTCGAGCATGACCGCATTAATGCCTTCCTTGTTTTCCTGCCGGGTATTCATAAATTTCAGCAAAGAAATCAATGTAGAGATCAATGCAGGTATAAAAATCACCCAACCCAAAATCCTTTTCAAAACAGCAATGCGTCCAGCTTGTTGATACGTCATGAGTTCTCCTTGATTAAGACGCGTCGTTTCACTTAGTTTACCTGTAGATATCTGTTTTCGCCCATTCTTTAAAGGCGATATGATAG  >  NC_000913/1809048‑1809319

cTGCATCATATCGATCATGACATGAGTAAAGTCGAGCATGACCGCATTAATGCCTTCCTTGTTTTCCTGCCGGGTATTCATAAATTTCAGCAAAGAAATCAATGTAGAGATCAATGCAGGTATAAAAATCacccagccca                                                                                                                                      >  1:76197/1‑140 (MQ=255)

   catcatATCGATCATGACATGAGTAAAGTCGAGCATGACCGCATTAATGCCTTCCTTGTTTTCCTGCCGGGTATTCATAAATTTCAGCAAAGAAATCAATGTAGAGATCAATGCAGGTATAAAAATCACCCAGCCCaaaa                                                                                                                                   >  1:712839/1‑140 (MQ=255)

             aTCATGACATGAGTAAAGTCGAGCATGACCGCATTAATGCCTTCCTTGTTTTCCTGCCGGGTATTCATAAATTTCAGCAAAGAAATCAATGTAGAGATCAATGCAGGTATAAAAATCACCCAGCCCAAAATCCTTTTCaa                                                                                                                         >  1:983411/1‑140 (MQ=255)

             aTCATGACATGAGTAAAGTCGAGCATGACCGCATTAATGCCTTCCTTGTTTTCCTGCCGGGTATTCATAAATTTCAGCAAAGAAATCAATGTAGAGATCAATGCAGGTATAAAAATCACCCAGCCCAAAATCCTTTTCaa                                                                                                                         >  2:682478/1‑140 (MQ=255)

                 tgacatgaGTAAAGTCGAGCATGACCGCATTAATGCCTTCCTTGTTTTCCTGCCGGGTATTCATAAATTTCAGCAAAGAAATCAATGTAGAGATCAATGCAGGTATAAAAATCACCCAGCCCAAAATCCTTTTCAAAACa                                                                                                                     >  1:753685/1‑140 (MQ=255)

                  gacatgaGTAAAGTCGAGCATGACCGCATTAATGCCTTCCTTGTTTTCCTGCCGGGTATTCATAAATTTCAGCAAAGAAATCAATGTAGAGATCAATGCAGGTATAAAAATCACCCAGCCCAAAATCCTTTTCAAAACAg                                                                                                                    >  2:941392/1‑140 (MQ=255)

                  gacatgaGTAAAGTCGAGCATGACCGCATTAATGCCTTCCTTGTTTTCCTGCCGGGTATTCATAAATTTCAGCAAAGAAATCAATGTAGAGATCAATGCAGGTATAAAAATCACCCAGCCCAAAATCCTTTTCAAAACAg                                                                                                                    >  1:509463/1‑140 (MQ=255)

                   acatgaGTAAAGTCGAGCATGACCGCATTAATGCCTTCCTTGTTTTCCTGCCGGGTATTCATAAATTTCAGCAAAGAAATCAATGTAGAGATCAATGCAGGTATAAAAATCACCCAGCCCAAAATCCTTTTCAAAACAGc                                                                                                                   >  2:451702/1‑140 (MQ=255)

                     atgaGTAAAGTCGAGCATGACCGCATTAATGCCTTCCTTGTTTTCCTGCCGGGTATTCATAAATTTCAGCAAAGAAATCAATGTAGAGATCAATGCAGGTATAAAAATCACCCAGCCCAAAATCCTTTTCAAAACAGCaa                                                                                                                 <  2:76197/140‑1 (MQ=255)

                      tgaGTAAAGTCGAGCATGACCGCATTAATGCCTTCCTTGTTTTCCTGCCGGGTATTCATAAATTTCAGCAAAGAAATCAATGTAGAGATCAATGCAGGTATAAAAATCACCCAGCCCAAAATCCTTTTCAAAACAGCAAt                                                                                                                <  2:545637/140‑1 (MQ=255)

                        aGTAAAGTCGAGCATGACCGCATTAATGCCTTCCTTGTTTTCCTGCCGGGTATTCATAAATTTCAGCAAAGAAATCAATGTAGAGATCAATGCAGGTATAAAAATCACCCAGCCCAAAATCCTTTTCAAAACAGCAATGc                                                                                                              >  1:930026/1‑140 (MQ=255)

                        aGTAAAGTCGAGCATGACCGCATTAATGCCTTCCTTGTTTTCCTGCCGGGTATTCATAAATTTCAGCAAAGAAATCAATGTAGAGATCAATGCAGGTATAAAAATCACCCAGCCCAAAATCCTTTTCAAAACAGCAATGc                                                                                                              >  2:292260/1‑140 (MQ=255)

                        aGTAAAGTCGAGCATGACCGCATTAATGCCTTCCTTGTTTTCCTGCCGGGTATTCATAAATTTCAGCAAAGAAATCAATGTAGAGATCAATGCAGGTATAAAAATCACCCAGCCCAAAATCCTTTTCAAAACAGCAATGc                                                                                                              >  2:709941/1‑140 (MQ=255)

                            aaGTCGAGCATGACCGCATTAATGCCTTCCTTGTTTTCCTGCCGGGTATTCATAAATTTCAGCAAAGAAATCAATGTAGAGATCAATGCAGGTATAAAAATCACCCAGCCCAAAATCCTTTTCAAAACAGCAATGCGTcc                                                                                                          >  1:147924/1‑140 (MQ=255)

                            aaGTCGAGCATGACCGCATTAATGCCTTCCTTGTTTTCCTGCCGGGTATTCATAAATTTCAGCAAAGAAATCAATGTAGAGATCAATGCAGGTATAAAAATCACCCAGCCCAAAATCCTTTTCAAAACAGCAATGCGTcc                                                                                                          >  2:433951/1‑140 (MQ=255)

                            aaGTCGAGCATGACCGCATTAATGCCTTCCTTGTTTTCCTGCCGGGTATTCATAAATTTCAGC‑AAGAAATCAATGTAGAGATCAATGCAGGTATAAAAATCACCCAGCCCAAAATCCTTTTCAAAACAGCAATGCGTCCa                                                                                                         >  1:707003/1‑140 (MQ=255)

                             aGTCGAGCATGACCGCATTAATGCCTTCCTTGTTTTCCTGCCGGGTATTCATAAATTTCAGCAAAGAAATCAATGTAGAGATCAATGCAGGTATAAAAATCACCCAGCCCAAAATCCTTTTCAAAACAGCAATGCGTCCa                                                                                                         >  2:964303/1‑140 (MQ=255)

                               tCGAGCATGACCGCATTAATGCCTTCCTTGTTTTCCTGCCGGGTATTCATAAATTTCAGCAAAGAAATCAATGTAGAGATCAATGCAGGTATAAAAATCACCCAGCCCAAAATCCTTTTCAAAACAGCAATGCGTCCAGc                                                                                                       <  1:143799/140‑1 (MQ=255)

                                  aGCATGACCGCATTAATGCCTTCCTTGTTTTCCTGCCGGGTATTCATAAATTTCAGCAAAGAAATCAATGTAGAGATCAATGCAGGTATAAAAATCACCCAGCCCAAAATCCTTTTCAAAACAGCAATGCGTCCAGCttg                                                                                                    <  1:709941/140‑1 (MQ=255)

                                  aGCATGACCGCATTAATGCCTTCCTTGTTTTCCTGCCGGGTATTCATAAATTTCAGCAAAGAAATCAATGTAGAGATCAATGCAGGTATAAAAATCACCCAGCCCAAAATCCTTTTCAAAACAGCAATGCGTCCAGCttg                                                                                                    >  1:522965/1‑140 (MQ=255)

                                  aGCATGACCGCATTAATGCCTTCCTTGTTTTCCTGCCGGGTATTCATAAATTTCAGCAAAGAAATCAATGTAGAGATCAATGCAGGTATAAAAATCACCCAGCCCAAAATCCTTTTCAAAACAGCAATGCGTCCAGCttg                                                                                                    <  1:292260/140‑1 (MQ=255)

                                    cATGACCGCATTAATGCCTTCCTTGTTTTCCTGCCGGGTATTCATAAATTTCAGCAAAGAAATCAATGTAGAGATCAATGCAGGTATAAAAATCACCCAGCCCAAAATCCTTTTCAAAACAGCAATGCGTCCAGCttgtt                                                                                                  >  1:859721/1‑140 (MQ=255)

                                       gACCGCATTAATGCCTTCCTTGTTTTCCTGCCGGGTATTCATAAATTTCAGCAAAGAAATCAATGTAGAGATCAATGCAGGTATAAAAATCACCCAGCCCAAAATCCTTTTCAAAACAGCAATGCGTCCAGCTTGTTGAt                                                                                               <  1:433951/140‑1 (MQ=255)

                                         ccGCATTAATGCCTTCCTTGTTTTCCTGCCGGGTATTCATAAATTTCAGCAAAGAAATCAATGTAGAGATCAATGCAGGTATAAAAATCACCCAGCCCAAAATCCTTTTCAAAACAGCAATGCGTCCAGCTTGTTGATAc                                                                                             <  2:763969/140‑1 (MQ=255)

                                           gCATTAATGCCTTCCTTGTTTTCCTGCCGGGTATTCATAAATTTCAGCAAAGAAATCAATGTAGAGATCAATGCAGGTATAAAAATCACCCAGCCCAAAATCCTTTTCAAAGCAGCAATGCGTCCAGCTTGTTGATACGt                                                                                           <  1:682478/140‑1 (MQ=255)

                                            cATTAATGCCTTCCTTGTTTTCCTGCCGGGTATTCATAAATTTCAGCAAAGAAATCAATGTAGAGATCAATGCAGGTATAAAAATCACCCAGCCCAAAATCCTTTTCAAAACAGCAATGCGTCCAGCTTGTTGATACGTc                                                                                          <  1:727494/140‑1 (MQ=255)

                                                 aTGCCTTCCTTGTTTTCCTGCCGGGTATTCATAAATTTCAGCAAAGAAATCAATGTAGAGATCAATGCAGGTATAAAAATCACCCAGCCCAAAATCCTTTTCAAAACAGCAATGCGTCCAGCTTGTTGATACGTCATGAg                                                                                     <  1:964303/140‑1 (MQ=255)

                                                  tGCCTTCCTTGTTTTCCTGCCGGGTATTCATAAATTTCAGCAAAGAAATCAATGTAGAGATCAATGCAGGTATAAAAATCACCCAGCCCAAAATCCTTTTCAAAACAGCAATGCGTCCAGCTTGTTGATACGTCATGAGt                                                                                    <  1:357080/140‑1 (MQ=255)

                                                  tGCCTTCCTTGTTTTCCTGCCGGGTATTCATAAATTTCAGCAAAGAAATCAATGTAGAGATCAATGCAGGTATAAAAATCACCCAGCCCAAAATCCTTTTCAAAACAGCAATGCGTCCAGCTTGTTGATACGTCATGAGt                                                                                    <  1:919815/140‑1 (MQ=255)

                                                  tGCCTTCCTTGTTTTCCTGCCGGGTATTCATAAATTTCAGCAAAGAAATCAATGTAGAGATCAATGCAGGTATAAAAATCACCCAGCCCAAAATCCTTTTCAAAACAGCAATGCGTCCAGCTTGTTGATACGTCATGAGt                                                                                    <  1:941392/140‑1 (MQ=255)

                                                       tccttGTTTTCCTGCCGGGTATTCATAAATTTCAGCAAAGAAATCAATGTAGAGATCAATGCAGGTATAAAAATCACCCAGCCCAAAATCCTTTTCAAAACAGCAATGCGTCCAGCTTGTTGATACGTCATGAGTTCTcc                                                                               <  2:147924/140‑1 (MQ=255)

                                                       tccttGTTTTCCTGCCGGGTATTCATAAATTTCAGCAAAGAAATCAATGTAGAGATCAATGCAGGTATAAAAATCACCCAGCCCAAAATCCTTTTCAAAACAGCAATGCGTCCAGCTTGTTGATACGTCATGAGTTCTcc                                                                               >  1:909333/1‑140 (MQ=255)

                                                                tCCTGCCGGGTATTCATAAATTTCAGCAAAGAAATCCATGTAGAGATCAATGCAGGTATAAAAATCACCCAGCCCAAACTCCTTTTCAAAACAGCAATGCGTCCGGCTTGTTGATACGTCATGAGTTCTCCTTGATTAAg                                                                      >  2:323973/1‑140 (MQ=255)

                                                                   tGCCGGGTATTCATAAATTTCAGCAAAGAAATCAATGTAGAGATCAATGCAGGTATAAAAATCACCCAGCCCAAAATCCTTTTCAAAACAGCAATGCGTCCAGCTTGTTGATACGTCATGAGTTCTCCTTGATTAAGAcg                                                                   <  2:753685/140‑1 (MQ=255)

                                                                               aTAAATTTCAGCAAAGAAATCAATGTAGAGATCAATGCAGGTATAAAAATCACCCAGCCCAAAATCCTTTTCAAAACTGCAATGCGTCCAGCTTGTTGATACGTCATGAGTTCTCCTTGATTAAGACGCGTCGTTTCACt                                                       <  2:909333/140‑1 (MQ=255)

                                                                                  aaTTTCAGC‑AAGAAATCAATGTAGAGATCAATGCAGGTATAAAAATCACCCAGCCCAAAATCCTTTTCAAAACAGCAATGCGTCCAGCTTGTTGATACGTCATGAGTTCTCCTTGATTAAGACGCGTCGTTTCACTTAGt                                                   <  2:707003/140‑1 (MQ=255)

                                                                                   aTTTCAGCAAAGAAATCAATGTAGAGATCAATGCAGGTATAAAAATCACCCAGCCCAAAATCCTTTTCAAAACAGCAATGCGTCCAGCTTGTTGATACGTCATGAGTTCTCCTTGATTAAGACGCGTCGTTTCACTTAGt                                                   >  2:493132/1‑140 (MQ=255)

                                                                                       cAGCAAAGAAATCAATGTAGAGATCAATGCAGGTATAAAAATCACCCAGCCCAAAATCCTTTTCAAAACAGCAATGCGTCCAGCTTGTTGATACGTCATGAGTTCTCCTTGATTAAGACGCGTCGTTTCACTTAGTTTAc                                               >  2:798781/1‑140 (MQ=255)

                                                                                             aGAAATCAATGTAGAGATCAATGCAGGTATAAAAATCACCCAGCCCAAAATCCTTTTCAAAACAGCAATGCGTCCAGCTTGTTGATACGTCATGAGTTCTCCTTGATTAAGACGCGTCGTTTCACTTAGTTTACCTGTAg                                         <  2:983411/140‑1 (MQ=255)

                                                                                               aaaTCAATGTAGAGATCAATGCAGGTATAAAAATCACCCAGCCCAAAATCCTTTTCAAAACAGCAATGCGTCCAGCTTGTTGATACGTCATGAGTTCTCCTTGATTAAGACGCGTCGTTTCACTTAGTTTACCTGTAGat                                       >  1:812010/1‑140 (MQ=255)

                                                                                                           agaTCAATGCAGGTATAAAAATCACCCAGCCCAAAATCCTTTTCAAAACAGCAATGCGTCCAGCTTGTTGATACGTCATGAGTTCTCCTTGATTAAGACGCGTCGTTTCACTTAGTTTACCTGTAGATATCTGTTTTCGc                           >  2:262886/1‑140 (MQ=255)

                                                                                                           agaTCAATGCAGGTATAAAAATCACCCAGCCCAAAATCCTTTTCAAAACAGCAATGCGTCCAGCTTGTTGATACGTCATGAGTTCTCCTTGATTAAGACGCGTCGTTTCACTTAGTTTACCTGTAGATATCTGTTTTCGc                           >  1:1009785/1‑140 (MQ=255)

                                                                                                            gaTCAATGCAGGTATAAAAATCACCCAGCCCAAAATCCTTTTCAAAACAGCAATGCGTCCAGCTTGTTGATACGTCATGAGTTCTCCTTGATTAAGACGCGTCGTTTCACTTAGTTTACCTGTAGATATCTGTTTTCGcc                          <  1:798781/140‑1 (MQ=255)

                                                                                                              tCAATGCAGGTATAAAAATCACCCAGCCCAAAATCCTTTTCAAAACAGCAATGCGTCCAGCTTGTTGATACGTCATGAGTTCTCCTTGATTAAGACGCGTCGTTTCACTTAGTTTACCTGTAGATATCTGTTTTCGCCCa                        <  2:509463/140‑1 (MQ=255)

                                                                                                              tCAATGCAGGTATAAAAATCACCCAGCCCAAAATCCTTTTCAAAACAGCAATGCGTCCAGCTTGTTGATACGTCATGAGTTCTCCTTGATTAAGACGCGTCGTTTCACTTAGTTTACCTGTAGATATCTGTTTTCGCCCa                        <  2:930026/140‑1 (MQ=255)

                                                                                                              tCAATGCAGGTATAAAAATCACCCAGCCCAAAATCCTTTTCAAAACAGCAATGCGTCCAGCTTGTTGATACGTCATGAGTTCTCCTTGATTAAGACGCGTCGTTTCACTTAGTTTACCTGTAGATATCTGTTTTCGCCCa                        <  1:493132/140‑1 (MQ=255)

                                                                                                                aaTGCAGGTATAAAAATCACCCAGCCCAAAATCCTTTTCAAAACAGCAATGCGTCCAGCTTGTTGATACGTCATGAGTTCTCCTTGATTAAGACGCGTCGTTTCACTTAGTTTACCTGTAGATATCTGTTTTCGCCCAtt                      >  2:818184/1‑140 (MQ=255)

                                                                                                                 aTGCAGGTATAAAAATCACCCAGCCCAAAATCCTTTTCAAAACAGCAATGCGTCCAGCTTGTTGATACGTCATGAGTTCTCCTTGATTAAGACGCGTCGTTTCACTTAGTTTACCTGTAGATATCTGTTTTCGCCCATTc                     >  1:816215/1‑140 (MQ=255)

                                                                                                                  tGCAGGTATAAAAATCACCCAGCCCAAAATCCTTTTCAAAACAGCAATGCGTCCAGCTTGTTGATACGTCATGAGTTCTCCTTGATTAAGACGCGTCGTTTCACTTAGTTTACCTGTAGATATCTGTTTTCGCCCATTCt                    >  1:23754/1‑140 (MQ=255)

                                                                                                                  tGCAGGTATAAAAATCACCCAGCCCAAAATCCTTTTCAAAACAGCAATGCGTCCAGCTTGTTGATACGTCATGAGTTCTCCTTGATTAAGACGCGTCGTTTCACTTAGTTTACCTGTAGATATCTGTTTTCGCCCATTCt                    >  2:532915/1‑140 (MQ=255)

                                                                                                                  tGCAGGTATAAAAATCACCCAGCCCAAAATCCTTTTCAAAACAGCAATGCGTCCAGCTTGTTGATACGTCATGAGTTCTCCTTGATTAAGACGCGTCGTTTCACTTAGTTTACCTGTAGATATCTGTTTTCGCCCATTCt                    >  1:451784/1‑140 (MQ=255)

                                                                                                                       gTATAAAAATCACCCAGCCCAAAATCCTTTTCAAAACAGCAATGCGTCCAGCTTGTTGATACGTCATGAGTTCTCCTTGATTAAGACGCGTCGTTTCACTTAGTTTACCTGTAGATATCTGTTTTCGCCCATTCTTTaaa               >  2:519852/1‑140 (MQ=255)

                                                                                                                       gTATAAAAATCACCCAGCCCAAAATCCTTTTCAAAACAGCAATGCGTCCAGCTTGTTGATACGTCATGAGTTCTCCTTGATTAAGACGCGTCGTTTCACTTAGTTTACCTGTAGATATCTGTTTTCGCCCATTCTTTaaa               <  2:126955/140‑1 (MQ=255)

                                                                                                                          taAAAATCACCCAGCCCAAAATCCTTTTCAAAACAGCAATGCGTCCAGCTTGTTGATACGTCATGAGTTCTCCTTGATTAAGACGCGTCGTTTCACTTAGTTTACCTGTAGATATCTGTTTTCGCCCATTCTTTAAAGGc            <  2:451784/140‑1 (MQ=255)

                                                                                                                                 cACCCAGCCCAAAATCCTTTTCAAAACAGCAATGCGTCCAGCTTGTTGATACGTCATGAGTTCTCCTTGATTAAGACGCGTCGTTTCACTTAGTTTACCTGTAGATATCTGTTTTCGCCCATTCTTTa                 >  2:269768/1‑128 (MQ=255)

                                                                                                                                 cACCCAGCCCAAAATCCTTTTCAAAACAGCAATGCGTCCAGCTTGTTGATACGTCATGAGTTCTCCTTGATTAAGACGCGTCGTTTCACTTAGTTTACCTGTAGATATCTGTTTTCGCCCATTCTTTa                 <  1:269768/128‑1 (MQ=255)

                                                                                                                                  acccagcccaAAATCCTTTTCAAAACAGCAATGCGTCCAGCTTGTTGATACGTCATGAGTTCTCCTTGATTAAGACGCGTCGTTTCACTTAGTTTACCTGTAGATATCTGTTTTCGCCCATTCTTTAAAGGCGATATGAt    >  1:947268/1‑140 (MQ=255)

                                                                                                                                   cccagcccaaAATCCTTTTCAAAACAGCAATGCGTCCAGCTTGTTGATACGTCATGAGTTCTCCTTGATTAAGACGCGTCGTTTCACTTAGTTTACCTGTAGATATCTGTTTTCGCCCATTCTTTAAAGGCGATATGATa   <  1:88568/140‑1 (MQ=255)

                                                                                                                                    ccagcccaaAATCCTTTTCAAAACAGCAATGCGTCCAGCTTGTTGATACGTCATGAGTTCTCCTTGATTAAGACGCGTCGTTTCACTTAGTTTACCTGTAGATATCTGTTTTCGCCCATTCTTTAAAGGCGATATGATAg  <  2:947268/140‑1 (MQ=255)

CTGCATCATATCGATCATGACATGAGTAAAGTCGAGCATGACCGCATTAATGCCTTCCTTGTTTTCCTGCCGGGTATTCATAAATTTCAGCAAAGAAATCAATGTAGAGATCAATGCAGGTATAAAAATCACCCAACCCAAAATCCTTTTCAAAACAGCAATGCGTCCAGCTTGTTGATACGTCATGAGTTCTCCTTGATTAAGACGCGTCGTTTCACTTAGTTTACCTGTAGATATCTGTTTTCGCCCATTCTTTAAAGGCGATATGATAG  >  NC_000913/1809048‑1809319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A