Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	155	60.3	2039550	99.3%	2025273	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,815,175	A→G	T437A (ACC→GCC)	katE →	catalase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,815,175	0	A	G	100.0%	197.3 / NA	56	T437A (ACC→GCC)	katE	catalase II
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (28/28); total (28/28)

GGCATATCGTGCCGGGACTGGACTTCACCAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGAAACGCGGC  >  NC_000913/1815047‑1815306

ggCATATCGTGCCGGGACTGGACTTCACCAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTAc                                                                                                                          >  2:29572/1‑140 (MQ=255)

         tGCCGGGACTGGACTTCACCAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTc                                                                                                                 <  1:353272/140‑1 (MQ=255)

          gCCGGGACTGGACTTCACCAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTcc                                                                                                                >  1:153319/1‑140 (MQ=255)

          gCCGGGACTGGACTTCACCAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTcc                                                                                                                >  2:566148/1‑140 (MQ=255)

                actggactTCACCAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTg                                                                                                          <  1:316285/140‑1 (MQ=255)

                   ggactTCACCAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTGACg                                                                                                       >  1:100987/1‑140 (MQ=255)

                   ggactTCACCAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTGACg                                                                                                       <  2:850930/140‑1 (MQ=255)

                   ggactTCACCAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTGACg                                                                                                       <  1:195398/140‑1 (MQ=255)

                        tCACCAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTGACGgcatg                                                                                                  <  2:67221/140‑1 (MQ=255)

                         cACCAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTg                                                                                                          >  1:111953/1‑131 (MQ=255)

                         cACCAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTg                                                                                                          <  2:111953/131‑1 (MQ=255)

                         cACCAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTGACGgcatgc                                                                                                 >  1:21879/1‑140 (MQ=255)

                           ccAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTGACGgcatgcat                                                                                               <  1:566148/140‑1 (MQ=255)

                                    cGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTGACGgcatgcat                                                                                               <  2:417821/131‑1 (MQ=255)

                                    cGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTGACGgcatgcat                                                                                               >  1:417821/1‑131 (MQ=255)

                                    cGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATTGCATgggg                                                                                      >  2:532885/1‑140 (MQ=255)

                                      cTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGAt                                                                                    <  1:961994/140‑1 (MQ=255)

                                          tGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGac                                                                                >  1:266388/1‑140 (MQ=255)

                                                 aCGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAAcc                                                                         >  1:497045/1‑140 (MQ=255)

                                                          ctcCTATACCGATACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAAtt                                                                >  1:198956/1‑140 (MQ=255)

                                                             cTATACCGATACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTAcg                                                             >  2:558317/1‑140 (MQ=255)

                                                                    gATACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTAcgaaccgaa                                                      <  2:791371/140‑1 (MQ=255)

                                                                     aTACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTAcgaaccgaac                                                     >  2:594926/1‑140 (MQ=255)

                                                                     aTACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTAcgaaccgaac                                                     >  2:156067/1‑140 (MQ=255)

                                                                     aTACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTAcgaaccgaac                                                     >  2:508496/1‑140 (MQ=255)

                                                                     aTACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTAcgaaccgaac                                                     >  2:412904/1‑140 (MQ=255)

                                                                     aTACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTAcgaaccgaac                                                     >  2:35596/1‑140 (MQ=255)

                                                                      tACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACt                                                    <  2:685558/140‑1 (MQ=255)

                                                                         acaAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGa                                                 <  1:792694/140‑1 (MQ=255)

                                                                         acaAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGa                                                 <  2:718483/140‑1 (MQ=255)

                                                                         acaAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGa                                                 <  2:100987/140‑1 (MQ=255)

                                                                          caAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGAt                                                <  1:35596/140‑1 (MQ=255)

                                                                             aTCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTAcgaaccg                                                        >  2:331038/1‑129 (MQ=255)

                                                                             aTCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTAcgaaccg                                                        <  1:331038/129‑1 (MQ=255)

                                                                              tCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGATTAAc                                            <  2:198956/140‑1 (MQ=255)

                                                                                aGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGATTAACGa                                          >  2:363210/1‑140 (MQ=255)

                                                                                  tcgtcTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGATTAACGATa                                        >  1:145418/1‑140 (MQ=255)

                                                                                       ttGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTgg                                   >  1:421086/1‑140 (MQ=255)

                                                                                       ttGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTgg                                   <  1:594926/140‑1 (MQ=255)

                                                                                         ggtggGCCGAATTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGcc                                 <  1:508496/140‑1 (MQ=255)

                                                                                               ccGAATTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGa                           >  2:417681/1‑140 (MQ=255)

                                                                                               ccGAATTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGa                           >  1:269994/1‑140 (MQ=255)

                                                                                               ccGAATTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGa                           <  1:156067/140‑1 (MQ=255)

                                                                                               ccGAATTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGa                           >  2:37298/1‑140 (MQ=255)

                                                                                               ccGAATTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGa                           >  2:605592/1‑140 (MQ=255)

                                                                                                cGAATTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGaa                          >  2:579194/1‑140 (MQ=255)

                                                                                                 gAATTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGaaa                         >  1:875194/1‑140 (MQ=255)

                                                                                                  aaTTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAac                        <  2:421086/140‑1 (MQ=255)

                                                                                                  aaTTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTGACGGCATACATCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAac                        <  1:29572/140‑1 (MQ=255)

                                                                                                   aTTTCCATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAaca                       <  1:417681/140‑1 (MQ=255)

                                                                                                       ccATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACAccgc                   <  1:412904/140‑1 (MQ=255)

                                                                                                       ccATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACAccgc                   <  1:37298/140‑1 (MQ=255)

                                                                                                       ccATGAGATTCCGATTAACCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACAccgc                   <  1:363210/140‑1 (MQ=255)

                                                                                                                       aaCCGTCCGGCCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGAAACgcgg   <  2:145418/140‑1 (MQ=255)

                                                                                                                            tCCGGCCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGAAACgcggc  <  1:816680/136‑1 (MQ=255)

                                                                                                                            tCCGGCCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGAAACgcggc  >  2:816680/1‑136 (MQ=255)

GGCATATCGTGCCGGGACTGGACTTCACCAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGAAACGCGGC  >  NC_000913/1815047‑1815306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A