Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	155	60.3	2039550	99.3%	2025273	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,864,892	C→T	V249I (GTC→ATC)	yeaE ←	methylglyoxal reductase YeaE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,864,892	0	C	T	100.0%	160.8 / NA	45	V249I (GTC→ATC)	yeaE	methylglyoxal reductase YeaE
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (22/23); total (22/23)

TGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTT  >  NC_000913/1864759‑1865013

tGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGATATGGGc                                                                                                                     >  2:789081/1‑140 (MQ=255)

 gCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGATATGGGCa                                                                                                                    >  1:986439/1‑140 (MQ=255)

          ccGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGATATGGGCAATCGTGGcc                                                                                                           <  2:748704/140‑1 (MQ=255)

             gCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGATATGGGCAATCGTGGCCGCt                                                                                                        >  1:148096/1‑140 (MQ=255)

                   aacaTCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGATATGGGCAATCGTGGCCGCTTTTGGa                                                                                                  <  2:148096/140‑1 (MQ=255)

                             cATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGATATGGGCAATCGTGGCCGCTTTTGGAATCGCCATca                                                                                        >  2:741927/1‑140 (MQ=255)

                              atatCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGATATGGGCAATCGTGGCCGCTTTTGGAATCGCCATcac                                                                                       <  1:233910/140‑1 (MQ=255)

                              atatCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGATATGGGCAATCGTGGCCGCTTTTGGAATCGCCATcac                                                                                       <  1:186974/140‑1 (MQ=255)

                              atatCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGATATGGGCAATCGTGGCCGCTTTTGGAATCGCCATcac                                                                                       <  2:132214/140‑1 (MQ=255)

                              atatCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGATATGGGCAATCGTGGCCGCTTTTGGAATCGCCATcac                                                                                       <  1:741927/140‑1 (MQ=255)

                                atCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGATATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCacac                                                                                     <  1:456148/140‑1 (MQ=255)

                                 tCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGATATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACAcc                                                                                    <  2:430901/140‑1 (MQ=255)

                                 tCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGATATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACAcc                                                                                    <  1:934215/140‑1 (MQ=255)

                                 tCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGATATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACAcc                                                                                    >  1:549312/1‑140 (MQ=255)

                                   cAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGATATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCt                                                                                  >  2:477086/1‑140 (MQ=255)

                                     gcgcAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGATATGGGCAATCGTGGCCGCTTTTGGAATCGCCa                                                                                           <  2:917085/129‑1 (MQ=255)

                                     gcgcAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGATATGGGCAATCGTGGCCGCTTTTGGAATCGCCa                                                                                           >  1:917085/1‑129 (MQ=255)

                                      cgcAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGATATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCtgat                                                                               <  1:419104/140‑1 (MQ=255)

                                       gcAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGATATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCtgat                                                                               <  2:1019440/139‑1 (MQ=255)

                                       gcAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGATATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCtgat                                                                               >  1:1019440/1‑139 (MQ=255)

                                             tttCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGATATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGAt                                                                        <  1:789081/140‑1 (MQ=255)

                                                 cTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGATATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCAcc                                                                    <  1:758166/140‑1 (MQ=255)

                                                    ttGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGATATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCAc                                                                 <  2:986439/140‑1 (MQ=255)

                                                       gTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGATATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGcc                                                              <  2:97534/140‑1 (MQ=255)

                                                                      tATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGATATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCAc                                                                 >  2:173119/1‑122 (MQ=255)

                                                                      tATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGATATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCAc                                                                 <  1:173119/122‑1 (MQ=255)

                                                                       aTCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGATATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCcg                                              >  2:965167/1‑140 (MQ=255)

                                                                        tCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGATATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCcgc                                             >  2:762155/1‑140 (MQ=255)

                                                                            gCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGATATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGc                                                >  1:895464/1‑133 (MQ=255)

                                                                            gCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGATATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGc                                                <  2:895464/133‑1 (MQ=255)

                                                                             cATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGATATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCcg                                              >  2:938738/1‑134 (MQ=255)

                                                                             cATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGATATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCcg                                              <  1:938738/134‑1 (MQ=255)

                                                                                  cTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGATATGGGCAATCGTGGCCGCTTTTGGAATAGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATtg                                   >  2:547918/1‑140 (MQ=255)

                                                                                      ttgcGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGATATGGGCAATCGTGGCTGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAg                               >  2:992737/4‑140 (MQ=255)

                                                                                       tCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGATATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCcgcg                                            <  2:773998/126‑1 (MQ=255)

                                                                                       tCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGATATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCcgcg                                            >  1:773998/1‑126 (MQ=255)

                                                                                         cGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGATATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCcg                                              <  2:759077/122‑1 (MQ=255)

                                                                                         cGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGATATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCcg                                              >  1:759077/1‑122 (MQ=255)

                                                                                          gCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGATATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCAtg                           >  2:393377/1‑140 (MQ=255)

                                                                                           cTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGATATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCAtgt                          >  1:931319/1‑140 (MQ=255)

                                                                                           cTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGATATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCAtgt                          >  1:250993/1‑140 (MQ=255)

                                                                                            tGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGATATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCAtgtg                         >  2:347061/1‑140 (MQ=255)

                                                                                                             cAAGCACAGCCGCATTTTGTTGGATATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTAcc        >  2:442491/1‑140 (MQ=255)

                                                                                                             cAAGCACAGCCGCATTTTGTTGGATATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTAcc        >  2:1008191/1‑140 (MQ=255)

                                                                                                              aaGCACAGCCGCATTTTGTTGGATATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACcg       <  2:250993/140‑1 (MQ=255)

                                                                                                                   caGCCGCATTTTGTTGGATATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGttt  <  2:931319/140‑1 (MQ=255)

TGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTT  >  NC_000913/1864759‑1865013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A