Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F28 I1 R1 151 87.4 2971636 99.2% 2947862 138.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,866,321 A→G V51A (GTA→GCA)  mipA ← scaffolding protein that interacts with murein polymerase and murein hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,866,3210AG98.5% 222.2 / ‑4.4 65V51A (GTA→GCA) mipAscaffolding protein that interacts with murein polymerase and murein hydrolase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (26/38);  total (27/38)
Fisher's exact test for biased strand distribution p-value = 4.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01

ATCGCCACTGTCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAA  >  NC_000913/1866185‑1866456
                                                                                                                                        |                                                                                                                                       
aTCGCCACTGTCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCg                                                                                                                                      <  2:967088/140‑1 (MQ=255)
  cGCCACTGTCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCGGt                                                                                                                                    <  2:902660/140‑1 (MQ=255)
  cGCCACTGTCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCGGt                                                                                                                                    <  2:1197949/140‑1 (MQ=255)
  cGCCACTGTCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCGGt                                                                                                                                    >  2:1347638/1‑140 (MQ=255)
  cGCCACTGTCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCGGt                                                                                                                                    >  1:303626/1‑140 (MQ=255)
   gCCACTGTCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCGGTa                                                                                                                                   <  2:965061/140‑1 (MQ=255)
   gCCACTGTCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCGGTa                                                                                                                                   <  2:883640/140‑1 (MQ=255)
   gCCACTGTCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCGGTa                                                                                                                                   <  2:252911/140‑1 (MQ=255)
       cTGTCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCg                                                                                                                                      <  2:983728/133‑1 (MQ=255)
       cTGTCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCg                                                                                                                                      >  1:983728/1‑133 (MQ=255)
       cTGTCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCAGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCGGTACTgg                                                                                                                               >  2:665195/1‑140 (MQ=255)
             ttCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCGGTACTGGGTAAAc                                                                                                                         <  1:25450/140‑1 (MQ=255)
               cGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCGGTACTGGGTAAACAt                                                                                                                       <  1:327034/140‑1 (MQ=255)
               cGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCGGTACTGGGTAAACAt                                                                                                                       >  2:327034/1‑140 (MQ=255)
               cGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCGGTACTGGGTAAACAt                                                                                                                       <  2:303626/140‑1 (MQ=255)
               cGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCGGTACTGGGTAAACAt                                                                                                                       <  2:209363/140‑1 (MQ=255)
               cGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCGGTACTGGGTAAACAt                                                                                                                       <  1:1347638/140‑1 (MQ=255)
                 cTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCGGTACTGGGTAAACATCg                                                                                                                     <  1:946771/140‑1 (MQ=255)
                  tttGAAGTAAAACGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCGGTACTGGGTAAACATCgg                                                                                                                    <  1:1473053/140‑1 (MQ=255)
                    tGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCGGTACTGGGTa                                                                                                                            >  1:681963/1‑130 (MQ=255)
                    tGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCGGTACTGGGTa                                                                                                                            <  2:681963/130‑1 (MQ=255)
                    tGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCGGTACTGGGTAAACATCGGTa                                                                                                                  >  1:1010361/1‑140 (MQ=255)
                    tGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCGGTACTGGGTAAACATCGGTa                                                                                                                  >  2:434382/1‑140 (MQ=255)
                           acgcggcgACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCGGTACTGGGTAAACATCGGTATCGTAAt                                                                                                           <  2:1010361/138‑1 (MQ=255)
                           aaGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCGGTACTGGGTAAACATCGGTATCGTAAt                                                                                                           <  2:952405/140‑1 (MQ=255)
                           aaGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCGGTACTGGGTAAACATCGGTATCGTAAt                                                                                                           <  2:725315/140‑1 (MQ=255)
                           aaGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCGGTACTGGGTAAACATCGGTATCGTAAt                                                                                                           <  1:434382/140‑1 (MQ=255)
                                gcgACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCGGTACTGGGTAAACATCGGTATCGTAAt                                                                                                           <  1:1009605/135‑1 (MQ=255)
                                gcgACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCGGTACTGGGTAAACATCGGTATCGTAAt                                                                                                           >  2:1009605/1‑135 (MQ=255)
                                   aCCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCGGTACTGGGTAAACATCGGTATCGTAAt                                                                                                           >  1:1099084/1‑132 (MQ=255)
                                   aCCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCGGTACTGGGTAAACATCGGTATCGTAAt                                                                                                           <  2:1099084/132‑1 (MQ=255)
                                   aCCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCGGTACTGGGTAAACATCGGTATCGTAATCTTTatat                                                                                                   >  1:841853/1‑140 (MQ=255)
                                     cAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATgg                                                                                                 <  1:1032028/140‑1 (MQ=255)
                                        tAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGgtg                                                                                              <  1:852743/140‑1 (MQ=255)
                                          aGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGGTGtt                                                                                            <  2:1396774/140‑1 (MQ=255)
                                               tAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACg                                                                                       >  2:433521/1‑140 (MQ=255)
                                                         tttATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGc                                                                             >  2:1027372/1‑140 (MQ=255)
                                                             tCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTgc                                                                         >  2:544745/1‑140 (MQ=255)
                                                                    cGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAgg                                                                  <  1:1027372/140‑1 (MQ=255)
                                                                           ccACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAAtt                                                           <  1:638275/140‑1 (MQ=255)
                                                                                ggTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCt                                                      <  2:261340/140‑1 (MQ=255)
                                                                                   agtaACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCa                                                   >  2:661988/1‑140 (MQ=255)
                                                                                    gtaACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAg                                                  <  1:544745/140‑1 (MQ=255)
                                                                                    gtaACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAg                                                  >  1:24431/1‑140 (MQ=255)
                                                                                           ccaccTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGc                                                 >  2:1456468/1‑134 (MQ=255)
                                                                                           ccaccTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGc                                                 <  1:1456468/134‑1 (MQ=255)
                                                                                            caccTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCt                                          >  1:54758/1‑140 (MQ=255)
                                                                                             accTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTa                                         >  1:721783/1‑140 (MQ=255)
                                                                                             accTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGCGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTa                                         <  2:881148/140‑1 (MQ=255)
                                                                                             accTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTGCCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGCGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTa                                         >  1:998769/1‑140 (MQ=255)
                                                                                                        ggAACCAGAAGTTATCGCCTTCATAGTTGATTGCCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGAc                              >  2:832383/1‑140 (MQ=255)
                                                                                                        ggAACCAGAAGTTATCGCCTTCATAGTTGATTGCCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGAc                              <  2:54758/140‑1 (MQ=255)
                                                                                                             cAGAAGTTATCGCCTTCATAGTTGATTGCCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGACGTTGc                         <  2:721783/140‑1 (MQ=255)
                                                                                                             cAGAAGTTATCGCCTTCATAGTTGATTGCCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGACGTTGc                         <  1:75250/140‑1 (MQ=255)
                                                                                                              aGAAGTTATCGCCTTCATAGTTGATTGCCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGACGTTGCg                        <  2:24431/140‑1 (MQ=255)
                                                                                                               gAAGTTATCGCCTTCATAGTTGATTGCCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGACGTTGCGa                       >  1:1401298/1‑140 (MQ=255)
                                                                                                               gAAGTTATCGCCTTCATAGTTGATTGCCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGACGTTGCGa                       >  2:1200029/1‑140 (MQ=255)
                                                                                                                aaGTTATCGCCTTCATAGTTGATTGCCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGACGTTGCGAt                      <  1:832383/140‑1 (MQ=255)
                                                                                                                   ttATCGCCTTCATAGTTGATTGCCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGACGTTGCGATAAg                   <  1:484097/140‑1 (MQ=255)
                                                                                                                    tATCGCCTTCATAGTTGATTGCCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGACGTTGCGa                       <  2:742958/135‑1 (MQ=255)
                                                                                                                    tATCGCCTTCATAGTTGATTGCCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGACGTTGCGa                       >  1:742958/1‑135 (MQ=255)
                                                                                                                          cTTCATAGTTGATTGCCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGACGTTGCGATAAGCACTCCa            >  2:1183280/1‑140 (MQ=255)
                                                                                                                           ttCATAGTTGATTGCCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGACg                             <  1:1257931/122‑1 (MQ=255)
                                                                                                                           ttCATAGTTGATTGCCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGACg                             >  2:1257931/1‑122 (MQ=255)
                                                                                                                                    gATTACCGGTACTGGGTAAACATCGGTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGaa  >  2:1438545/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                       
ATCGCCACTGTCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAA  >  NC_000913/1866185‑1866456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A