Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	155	60.3	2039550	99.3%	2025273	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,873,384	A→G	K448K (AAA→AAG)	dgcJ →	putative diguanylate cyclase DgcJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,873,384	0	A	G	100.0%	193.3 / NA	55	K448K (AAA→AAG)	dgcJ	putative diguanylate cyclase DgcJ
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (27/28); total (27/28)

CGTTATTAGCTCAGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAACGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGCAGAACAAA  >  NC_000913/1873248‑1873516

cGTTATTAGCTCAGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGt                                                                                                                                   <  2:531857/140‑1 (MQ=255)

cGTTATTAGCTCAGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGt                                                                                                                                   <  2:626898/140‑1 (MQ=255)

cGTTATTAGCTCAGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGt                                                                                                                                   <  2:530472/140‑1 (MQ=255)

cGTTATTAGCTCAGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGt                                                                                                                                   <  2:755981/140‑1 (MQ=255)

    attaGCTCAGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGc                                                                                                                               <  1:834070/140‑1 (MQ=255)

         cTCAGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACat                                                                                                                          >  1:585486/1‑140 (MQ=255)

          tCAGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGGATTCTGCATCATTCTTGTCGACTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGt                                                                                                                                   <  1:269391/130‑1 (MQ=255)

          tCAGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGGATTCTGCATCATTCTTGTCGACTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGt                                                                                                                                   >  2:269391/1‑130 (MQ=255)

             ggCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATcg                                                                                                                      >  1:40873/1‑140 (MQ=255)

              gCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATcgc                                                                                                                     <  1:273032/140‑1 (MQ=255)

               cGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATcgcg                                                                                                                    >  2:137142/1‑140 (MQ=255)

                   tAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGcgccgc                                                                                                                >  1:849934/1‑140 (MQ=255)

                        aGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGaaa                                                                                                           >  1:70588/1‑140 (MQ=255)

                          tCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAaga                                                                                                         >  2:493072/1‑140 (MQ=255)

                          tCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAaga                                                                                                         >  1:791815/1‑140 (MQ=255)

                            gATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAgaga                                                                                                       <  2:294798/140‑1 (MQ=255)

                            gATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAgaga                                                                                                       >  1:953815/1‑140 (MQ=255)

                              ttCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATc                                                                                                     <  2:244575/140‑1 (MQ=255)

                                   aaaaGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCtt                                                                                                <  2:565611/140‑1 (MQ=255)

                                         gATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTc                                                                                          <  2:308707/140‑1 (MQ=255)

                                          aTTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTcc                                                                                         >  1:1010940/1‑140 (MQ=255)

                                              tGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTa                                                                                     <  2:224520/140‑1 (MQ=255)

                                                  aTCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTa                                                                                 <  2:40873/140‑1 (MQ=255)

                                                   tCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAGCGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTAc                                                                                >  2:630650/1‑140 (MQ=255)

                                                   tCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGCATTTAc                                                                                >  1:151328/1‑140 (MQ=255)

                                                    ccGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTAcg                                                                               >  1:807835/1‑140 (MQ=255)

                                                    ccGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTAcg                                                                               >  1:611869/1‑140 (MQ=255)

                                                        cTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAGGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGAt                                                                           >  1:130747/1‑140 (MQ=255)

                                                        cTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGAt                                                                           >  2:133396/1‑140 (MQ=255)

                                                        cTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGAt                                                                           >  1:59331/1‑140 (MQ=255)

                                                                 gATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGaaaa                                                                  <  2:849934/140‑1 (MQ=255)

                                                                 gATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGaaaa                                                                  <  1:126030/140‑1 (MQ=255)

                                                                       ttattcatcatTCGTGTCGATACGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCATCATATCGCGCCGCAGAAAGAGATCGGCTTCACTCCCGGTATTTACGCGATGAAAGAAAACGATAc                                                            <  2:151328/135‑1 (MQ=255)

                                                                       ttCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCACAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATAc                                                            >  2:407879/1‑140 (MQ=255)

                                                                         cTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGt                                                          <  1:133396/140‑1 (MQ=255)

                                                                            catcatTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTAc                                                       <  1:137142/140‑1 (MQ=255)

                                                                              tcatTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACat                                                     <  2:368132/140‑1 (MQ=255)

                                                                                 ttCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACatgat                                                  <  2:539766/140‑1 (MQ=255)

                                                                                 ttCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACatgat                                                  <  2:953815/140‑1 (MQ=255)

                                                                                 ttCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACatgat                                                  <  2:611869/140‑1 (MQ=255)

                                                                                 ttCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACatgat                                                  >  2:268031/1‑140 (MQ=255)

                                                                                 ttCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACatgat                                                  >  2:120223/1‑140 (MQ=255)

                                                                                      gTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGta                                             <  1:405050/140‑1 (MQ=255)

                                                                                           ttCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACat                                                     <  1:772650/127‑1 (MQ=255)

                                                                                           ttCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACat                                                     >  2:772650/1‑127 (MQ=255)

                                                                                           ttCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAg                                        >  1:736286/1‑140 (MQ=255)

                                                                                            tCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGc                                       >  2:587624/1‑140 (MQ=255)

                                                                                            tCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGc                                       >  1:482035/1‑140 (MQ=255)

                                                                                                  ccgcAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGa                                 <  2:791815/140‑1 (MQ=255)

                                                                                                   cgcAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCACAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGAt                                <  1:407879/140‑1 (MQ=255)

                                                                                                     cAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGa                              >  2:180906/1‑140 (MQ=255)

                                                                                                              gcaCAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTtata                     <  2:736286/140‑1 (MQ=255)

                                                                                                                         tGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGc          <  2:807835/140‑1 (MQ=255)

                                                                                                                            aCGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGCAGa       >  2:316221/1‑140 (MQ=255)

                                                                                                                                 tCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGCAGAACaaa  >  2:224813/1‑140 (MQ=255)

                                                                                                                                 tCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGCAGAACaaa  <  1:224813/140‑1 (MQ=255)

CGTTATTAGCTCAGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAACGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGCAGAACAAA  >  NC_000913/1873248‑1873516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A