Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	155	60.3	2039550	99.3%	2025273	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,957,452	T→C	K227K (AAA→AAG)	torY ←	cytochrome c quinol dehydrogenase TorY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,957,452	0	T	C	100.0%	168.3 / NA	48	K227K (AAA→AAG)	torY	cytochrome c quinol dehydrogenase TorY
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (22/26); total (22/26)

GCCATTCCCCGTTTACAGTGAGCGCCTTGATACCTTCTTCACTTAATGTGGCTAGCGCCAGCCGTTGACCTGCCGCCAGGTAGAGCGTGTTTTCACTGCCCTGCATTTGATAACCCTGCAACAGAACGATCGGCTGTTTGCCACTGGCATCAACGACGGTGAGATCGGTTCCAGGATTCACGGTAGCCAGCTCGCCTATGTGAGAAGTTTTGAAAGGATAAATATGTGCGCCATTAGTAACTGAAGTAGCGGCCTGACTTTCCAGCTCATGCG  >  NC_000913/1957316‑1957588

gCCATTCCCCGTTTACAGTGAGCGCCTTGATACCTTCTTCACTTAATGTGGCTAGCGCCAGCCGTTGACCTGCCGCCAGGTAGAGCGTGTTTTCACTGCCCTGCATTTGATAACCCTGCAACAGAACGATCGGCTGCTTg                                                                                                                                       >  1:116027/1‑140 (MQ=255)

         cGTTTACAGTGAGCGCCTTGATACCTTCTTCACTTAATGTGGCTAGCGCCAGCCGTTGACCTGCCGCCAGGTAGAGCGTGTTTTCACTGCCCTGCATTTGATAACCCTGCAACAGAACGATCGGCTGCTTGCCACTGGCa                                                                                                                              <  1:155347/140‑1 (MQ=255)

                 gTGAGCGCCTTGATACCTTCTTCACTTAATGTGGCTAGCGCCAGCCGTTGACCTGCCGCCAGGTAGAGCGTGTTTTCACTGCCCTGCATTTGATAACCCTGCAACAGAACGATCGGCTGCTTGCCACTGGCATCAacgac                                                                                                                      >  2:130660/1‑140 (MQ=255)

                    aGCGCCTTGATACCTTCTTCACTTAATGTGGCTAGCGCCAGCCGTTGACCTGCCGCCAGGTAGAGCGTGTTTTCACTGCCCTGCATTTGATAACCCTGCAACAGAACGATCGGCTGCTTGCCACTGGCATCAACGACGGt                                                                                                                   >  2:64129/1‑140 (MQ=255)

                     gcgcCTTGATACCTTCTTCACTTAATGTGGCTAGCGCCAGCCGTTGACCTGCCGCCAGGTAGAGCGTGTTTTCACTGCCCTGCATTTGATAACCCTGCAACAGAACGATCGGCTGCTTGCCACTGGCATCAACGACGGTg                                                                                                                  <  1:88689/140‑1 (MQ=255)

                         cTTGATACCTTCTTCACTTAATGTGGCTAGCGCCAGCCGTTGACCTGCCGCCAGGTAGAGCGTGTTTTCACTGCCCTGCATTTGATAACCCTGCAACAGAACGATCGGCTGCTTGCCACTGGCATCAACGACGGTGAGAt                                                                                                              <  1:622234/140‑1 (MQ=255)

                         cTTGATACCTTCTTCACTTAATGTGGCTAGCGCCAGCCGTTGACCTGCCGCCAGGTAGAGCGTGTTTTCACTGCCCTGCATTTGATAACCCTGCAACAGAACGATCGGCTGCTTGCCACTGGCATCAACGACGGTGAGAt                                                                                                              <  2:546113/140‑1 (MQ=255)

                          ttGATACCTTCTTCACTTAATGTGGCTAGCGCCAGCCGTTGACCTGCCGCCAGGTAGAGCGTGTTTTCACTGCCCTGCATTTGATAACCCTGCAACAGAACGATCGGCTGCTTGCCACTGGCATCAACGACGGTGAGATc                                                                                                             >  2:392991/1‑140 (MQ=255)

                               aCCTTCTTCACTTAATGTGGCTAGCGCCAGCCGTTGACCTGCCGCCAGGTAGAGCGTGTTTTCACTGCCCTGCATTTGATAACCCTGCAACAGAACGATCGGCTGCTTGCCACTGGCATCAACGACGGTGAGATCGGTTc                                                                                                        <  2:869126/140‑1 (MQ=255)

                                   tcttcACTTAATGTGGCTAGCGCCAGCCGTTGACCTGCCGCCAGGTAGAGCGTGTTTTCACTGCCCTGCATTTGATAACCCTGCAACAGAACGATCGGCTGCTTGCCACTGGCATCAACGACGGTGAGATCGGTTCCAgg                                                                                                    >  1:764554/1‑140 (MQ=255)

                                    cttcACTTAATGTGGCTAGCGCCAGCCGTTGACCTGCCGCCAGGTAGAGCGTGTTTTCACTGCCCTGCATTTGATAACCCTGCAACAGAACGATCGGCTGCTTGCCACTGGCATCAACGACGGTGAGATCGGTTCCAGGa                                                                                                   >  2:873115/1‑140 (MQ=255)

                                     ttcACTTAATGTGGCTAGCGCCAGCCGTTGACCTGCCGCCAGGTAGAGCGTGTTTTCACTGCCCTGCATTTGATAACCCTGCAACAGAACGATCGGCTGCTTGCCACTGGCATCAACGACGGTGAGATCGGTTCCAGGAt                                                                                                  >  2:468614/1‑140 (MQ=255)

                                     ttcACTTAATGTGGCTAGCGCCAGCCGTTGACCTGCCGCCAGGTAGAGCGTGTTTTCACTGCCCTGCATTTGATAACCCTGCAACAGAACGATCGGCTGCTTGCCACTGGCATCAACGACGGTGAGATCGGTTCCAGGAt                                                                                                  >  2:708443/1‑140 (MQ=255)

                                        aCTTAATGTGGCTAGCGCCAGCCGTTGACCTGCCGCCAGGTAGAGCGTGTTTTCACTGCCCTGCATTTTATAACCCTGCAACAGAACGATCGGCTGCTTGCCACTGGCATCAACGACGGTGAGATCGGTTCCAGGATTCa                                                                                               <  1:77292/140‑1 (MQ=255)

                                          ttAATGTGGCTAGCGCCAGCCGTTGACCTGCCGCCAGGTAGAGCGTGTTTTCACTGCCCTGCATTTGATAACCCTGCAACAGAACGATCGGCTGCTTGCCACTGGCATCAACGACGGTGAGATCGGTTCCAGGATTCACg                                                                                             <  2:78515/140‑1 (MQ=255)

                                             aTGTGGCTAGCGCCAGCCGTTGACCTGCCGCCAGGTAGAGCGTGTTTTCACTGCCCTGCATTTGATAACCCTGCAACAGAACGATCGGCTGCTTGCCACTGGCATCAACGACGGTGAGATCGGTTCCAGGAt                                                                                                  >  1:321197/1‑132 (MQ=255)

                                             aTGTGGCTAGCGCCAGCCGTTGACCTGCCGCCAGGTAGAGCGTGTTTTCACTGCCCTGCATTTGATAACCCTGCAACAGAACGATCGGCTGCTTGCCACTGGCATCAACGACGGTGAGATCGGTTCCAGGAt                                                                                                  <  2:321197/132‑1 (MQ=255)

                                                   cTAGCGCCAGCCGTTGACCTGCCGCCAGGTAGAGCGTGTTTTCACTGCCCTGCATTTGATAACCCTGCAACAGAACGATCGGCTGCTTGCCACTGGCATCAACGACGGTGAGATCGGTTCCAGGATTCACGGTAGCCAGc                                                                                    <  1:308141/140‑1 (MQ=255)

                                                      gcgcCAGCCGTTGACCTGCCGCCAGGTAGAGCGTGTTTTCACTGCCCTGCATTTGATAACCCTGCAACAGAACGATCGGCTGCTTGCCACTGGCATCAACGACGGTGAGATCGGTTCCAGGATTCACGGTAGCCAGCTCg                                                                                 >  1:965917/1‑140 (MQ=255)

                                                       cgcCAGCCGTTGACCTGCCGCCAGGTAGAGCGTGTTTTCACTGCCCTGCATTTGATAACCCTGCAACAGAACGATCGGCTGCTTGCCACTGGCATCAACGACGGTGAGATCGGTTCCAGGATTCACGGTAGCCAGCTCGc                                                                                >  1:656291/1‑140 (MQ=255)

                                                       cgcCAGCCGTTGACCTGCCGCCAGGTAGAGCGTGTTTTCACTGCCCTGCATTTGATAACCCTGCAACAGAACGATCGGCTGCTTGCCACTGGCATCAACGACGGTGAGATCGGTTCCAGGATTCACGGTAGCCAGCTCGc                                                                                <  1:392991/140‑1 (MQ=255)

                                                            gCCGTTGACCTGCCGCCAGGTAGAGCGTGTTTTCACTGCCCTGCATTTGATAACCCTGCAACAGAACGATCGGCTGCTTGCCACTGGCATCAACGACGGTGAGATCGGTTCCAGGATTCACGGTAGCCAGCTCGCCTAtg                                                                           >  1:949466/1‑140 (MQ=255)

                                                            gCCGTTGACCTGCCGCCAGGTAGAGCGTGTTTTCACTGCCCTGCATTTGATAACCCTGCAACAGAACGATCGGCTGCTTGCCACTGGCATCAACGACGGTGAGATCGGTTCCAGGATTCACGGTAGCCAGCTCGCCTAtg                                                                           >  1:170399/1‑140 (MQ=255)

                                                                       gccgccAGGTAGAGCGTGTTTTCACTGCCCTGCATTTGATAACCCTGCAACAGAACGATCGGCTGCTTGCCACTGGCATCAACGACGGTGAGATCGGTTCCAGGATTCACGGTAGCCAGCTCGCCTATGTGAGAAGtttt                                                                >  1:679285/1‑140 (MQ=255)

                                                                         cgccAGGTAGAGCGTGTTTTCACTGCCCTGCATTTGATAACCCTGCAACAGAACGATCGGCTGCTTGCCACTGGCATCAACGACGGTGAGATCGGTTCCAGGATTCACGGTAGCCAGCTCGCCTATGTGAGAAGTTTTGa                                                              <  2:116027/140‑1 (MQ=255)

                                                                         cgccAGGTAGAGCGTGTTTTCACTGCCCTGCATTTGATAACCCTGCAACAGAACGATCGGCTGCTTGCCACTGGCATCAACGACGGTGAGATCGGTTCCAGGATTCACGGTAGCCAGCTCGCCTATGTGAGAAGTTTTGa                                                              <  2:656291/140‑1 (MQ=255)

                                                                         cgccAGGTAGAGCGTGTTTTCACTGCCCTGCATTTGATAACCCTGCAACAGAACGATCGGCTGCTTGCCACTGGCATCAACGACGGTGAGATCGGTTCCAGGATTCACGGTAGCCAGCTCGCCTATGTGAGAAGTTTTGa                                                              <  2:619283/140‑1 (MQ=255)

                                                                         cgccAGGTAGAGCGTGTTTTCACTGCCCTGCATTTGATAACCCTGCAACAGAACGATCGGCTGCTTGCCACTGGCATCAACGACGGTGAGATCGGTTCCAGGATTCACGGTAGCCAGCTCGCCTATGTGAGAAGTTTTGa                                                              <  2:170399/140‑1 (MQ=255)

                                                                         cgccAGGTAGAGCGTGTTTTCACTGCCCTGCATTTGATAACCCTGCAACAGAACGATCGGCTGCTTGCCACTGGCATCAACGACGGTGAGATCGGTTCCAGGATTCACGGTAGCCAGCTCGCCTATGTGAGAAGTTTTGa                                                              <  1:708443/140‑1 (MQ=255)

                                                                          gccAGGTAGAGCGTGTTTTCACTGCCCTGCATTTGATAACCCTGCAACAGAACGATCGGCTGCTTGCCACTGGCATCAACGACGGTGAGATCGGTTCCAGGATTCACGGTAGCCAGCTCGCCTATGTGAGAAGTTTTGaa                                                             >  2:904424/1‑140 (MQ=255)

                                                                           ccAGGTAGAGCGTGTTTTCACTGCCCTGCATTTGATAACCCTGCAACAGAACGATCGGCTGCTTGCCACTGGCATCAACGACGGTGAGATCGGTTCCAGGATTCACGGTAGCCAGCTCGCCTATGTGAGAAGTTTTGaaa                                                            <  2:441827/140‑1 (MQ=255)

                                                                            cAGGTAGAGCGTGTTTTCACTGCCCTGCATTTGATAACCCTGCAACAGAACGATCGGCTGCTTGCCACTGGCATCAACGACGGTGAGATCGGTTCCAGGATTCACGGTAGCCAGCTCGCCTATGTGAGAAGTTTTGAAAg                                                           <  2:949466/140‑1 (MQ=255)

                                                                            cAGGTAGAGCGTGTTTTCACTGCCCTGCATTTGATAACCCTGCAACAGAACGATCGGCTGCTTGCCACTGGCATCAACGACGGTGAGATCGGTTCCAGGATTCACGGTAGCCAGCTCGCCTATGTGAGAAGTTTTGAAAg                                                           >  1:344367/1‑140 (MQ=255)

                                                                               gTAGAGCGTGTTTTCACTGCCCTGCATTTGATAACCCTGCAACAGAACGATCGGCTGCTTGCCACTGGCATCAACGACGGTGAGATCGGTTCCAGGATTCACGGTAGCCAGCTCGCCTATGTGAGAAGTTTTGAAAGGAt                                                        <  1:873115/140‑1 (MQ=255)

                                                                                       tgtTTTCACTGCCCTGCATTTGATAACCCTGCAACAGAACGATCGGCTGCTTGCCACTGGCATCAACGACGGTGAGATCGGTTCCAGGATTCACGGTAGCCAGCTCGCCTATGTGAGAAGTTTTGAAAGGATAAATAtgt                                                <  2:344367/140‑1 (MQ=255)

                                                                                        gtTTTCACTGCCCTGCATTTGATAACCCTGCAACAGAACGATCGGCTGCTTGCCACTGGCATCAACGACGGTGAGATCGGTTCCAGGATTCACGGTAGCCAGCTCGCCTATGTGAGAAGTTTTGAAAGGATAAATAtgtg                                               >  2:428532/1‑140 (MQ=255)

                                                                                          tttCACTGCCCTGCATTTGATAACCCTGCAACAGAACGATCGGCTGCTTGCCACTGGCATCAACGACGGTGAGATCGGTTCCAGGATTCACGGTAGCCAGCTCGCCTATGTGAGAAGTTTTGa                                                              <  2:965237/123‑1 (MQ=255)

                                                                                          tttCACTGCCCTGCATTTGATAACCCTGCAACAGAACGATCGGCTGCTTGCCACTGGCATCAACGACGGTGAGATCGGTTCCAGGATTCACGGTAGCCAGCTCGCCTATGTGAGAAGTTTTGa                                                              >  1:965237/1‑123 (MQ=255)

                                                                                          tttCACTGCCCTGCATTTGATAACCCTGCAACAGAACGATCGGCTGCTTGCCACTGGCATCAACGACGGTGAGATCGGTTCCAGGATTCACGGTAGCCAGCTCGCCTATGTGAGAAGTTTTGAAAGGATAAATATGTgcg                                             <  1:904424/140‑1 (MQ=255)

                                                                                          tttCACTGCCCTGCATTTGATAACCCTGCAACAGAACGATCGGCTGCTTGCCACTGGCATCAACGACGGTGAGATCGGTTCCAGGATTCACGGTAGCCAGCTCGCCTATGTGAGAAGTTTTGAAAGGATAAATATGTgcg                                             <  1:468614/140‑1 (MQ=255)

                                                                                                tGCCCTGCATTTGATAACCCTGCAACAGAACGATCGGCTGCTTGCCACTGGCATCAACGACGGTGAGATCGGTTCCAGGATTCACGGTAGCCAGCTCGCCTATGTGAGAAGTTTTGAAAGGATAAATATGTGCGCCATTa                                       <  2:223400/140‑1 (MQ=255)

                                                                                                tGCCCTGCATTTGATAACCCTGCAACAGAACGATCGGCTGCTTGCCACTGGCATCAACGACGGTGAGATCGGTTCCAGGATTCACGGTAGCCAGCTCGCCTATGTGAGAAGTTTTGAAAGGATAAATATGTGCGCCATTa                                       <  1:98254/140‑1 (MQ=255)

                                                                                                tGCCCTGCATTTGATAACCCTGCAACAGAACGATCGGCTGCTTGCCACTGGCATCAACGACGGTGAGATCGGTTCCAGGATTCACGGTAGCCAGCTCGCCTATGTGAGAAGTTTTGAAAGGATAAATATGTGCGCCATTa                                       <  2:1015688/140‑1 (MQ=255)

                                                                                                            gATAACCCTGCAACAGAACGATCGGCTGCTTGCCACTGGCATCAACGACGGTGGGATCGGTTCCAGGATTCACGGTAGCCAGCTCGCCTATGTGAGAAGTTTTGAAAGGATAAATATGTGCGCCATTAGTAACTGAAGTa                           >  1:950095/1‑140 (MQ=255)

                                                                                                                    tGCAACAGAACGATCGGCTGCTTGCCACTGGCATCAACGACGGTGAGATCGGTTCCAGGATTCACGGTAGCCAGCTCGCCTATGTGAGAAGTTTTGAAAGGATAAATATGTGCGCCATTAGTAACTGAAGTAGCGGCCTg                   >  2:392889/1‑140 (MQ=255)

                                                                                                                            aaCGATCGGCTGCTTGCCACTGGCATCAACGACGGTGAGATCGGTTCCAGGATTCACGGTAGCCAGCTCGCCTATGTGAGAAGTTTTGAAAGGATAAATATGTGCGCCATTAGTAACTGAAGTAGCGGCCTGACTTTCCa           >  2:960592/1‑140 (MQ=255)

                                                                                                                               gATCGGCTGCTTGCCACTGGCATCAACGACGGTGAGATCGGCTCCAGGATTCACGGTAGCCAGCTCGCCTATGTGAGAAGTTTTGAAAGGATAAATATGTGCGCCATTAGTAACTGAAGTAGCGGCCTGACTTTCCAGCt        >  1:867391/1‑140 (MQ=255)

                                                                                                                                     cTGCTTGCCACTGGCATCAACGACGGTGAGATCGGTTCCAGGATTCACGGTAGCCAGCTCGCCTATGTGAGAAGTTTTGAAAGGATAAATATGTGCGCCATTAGTAACTGAAGTAGCGGCCTGACTTTCCAGCTCATgcg  <  2:965917/140‑1 (MQ=255)

GCCATTCCCCGTTTACAGTGAGCGCCTTGATACCTTCTTCACTTAATGTGGCTAGCGCCAGCCGTTGACCTGCCGCCAGGTAGAGCGTGTTTTCACTGCCCTGCATTTGATAACCCTGCAACAGAACGATCGGCTGTTTGCCACTGGCATCAACGACGGTGAGATCGGTTCCAGGATTCACGGTAGCCAGCTCGCCTATGTGAGAAGTTTTGAAAGGATAAATATGTGCGCCATTAGTAACTGAAGTAGCGGCCTGACTTTCCAGCTCATGCG  >  NC_000913/1957316‑1957588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A