Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F28 I1 R1 155 60.3 2039550 99.3% 2025273 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,257,717 A→G R217R (CGT→CGC)  psuG ← pseudouridine‑5'‑phosphate glycosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,257,7170AG97.5% 272.7 / ‑2.2 80R217R (CGT→CGC) psuGpseudouridine‑5'‑phosphate glycosidase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (40/38);  total (41/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

GAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGATAGTGTGTTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCACGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACGCAGGCAGCGCTTTAGTCTGATAGCCAATTAACGGCACACCGAAGGTTTCTAAATACTCAGTGGTTAAT  >  NC_000913/2257584‑2257852
                                                                                                                                     |                                                                                                                                       
gAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGATAGTGTGTTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCGCGGGCa                                                                                                                                   >  1:524763/1‑140 (MQ=255)
       agcttcagctACCGCCTGATCGATCGCCGCATTGATAGTGTGTTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGc                                                                                                                            >  1:403229/1‑140 (MQ=255)
                 aCCGCCTGATCGATCGCCGCATTGATAGTGTGTTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTc                                                                                                                  <  1:245876/140‑1 (MQ=255)
                       tGATCGATCGCCGCATTGATAGTGTGTTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACg                                                                                                            <  2:84705/140‑1 (MQ=255)
                       tGATCGATCGCCGCATTGATAGTGTGTTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACg                                                                                                            <  1:626073/140‑1 (MQ=255)
                         atcgatcgCCGCATTGATAGTGTGTTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGaa                                                                                                          >  2:364311/1‑140 (MQ=255)
                             atcgCCGCATTGATAGTGTGTTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCt                                                                                                      >  1:608221/1‑140 (MQ=255)
                             atcgCCGCATTGATAGTGTGTTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCt                                                                                                      >  2:830208/1‑140 (MQ=255)
                             atcgCCGCATTGATAGTGTGTTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCt                                                                                                      >  1:31362/1‑140 (MQ=255)
                             atcgCCGCATTGATAGTGTGTTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCt                                                                                                      >  1:378045/1‑140 (MQ=255)
                              tcgCCGCATTGATAGTGTGTTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTg                                                                                                     >  1:201262/1‑140 (MQ=255)
                                gccgcATTGATAGTGTGTTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCACGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGAc                                                                                                   >  1:489250/1‑140 (MQ=255)
                                     aTTGATAGTGTGTTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCaa                                                                                              <  1:113336/140‑1 (MQ=255)
                                           aGTGTGTTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGc                                                                                        <  2:788876/140‑1 (MQ=255)
                                               tgtTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGt                                                                                    >  1:583508/1‑140 (MQ=255)
                                               tgtTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGt                                                                                    >  1:805262/1‑140 (MQ=255)
                                               tgtTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGt                                                                                    >  1:788363/1‑140 (MQ=255)
                                               tgtTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGt                                                                                    >  1:708301/1‑140 (MQ=255)
                                               tgtTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGt                                                                                    >  1:399451/1‑140 (MQ=255)
                                               tgtTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGt                                                                                    >  1:983193/1‑140 (MQ=255)
                                                gtTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTg                                                                                   <  2:201262/140‑1 (MQ=255)
                                                gtTCCGGCATAGCAAACTGTTCCGGGA‑CGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGc                                                                                  >  1:903063/1‑140 (MQ=255)
                                                 ttCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGc                                                                                  >  1:478439/1‑140 (MQ=255)
                                                 ttCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGc                                                                                  >  1:814430/1‑140 (MQ=255)
                                                  tCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCg                                                                                 <  1:114608/140‑1 (MQ=255)
                                                   ccGGCATAGCAAACTGTTCCGGGATTGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCgg                                                                                >  1:976972/1‑140 (MQ=255)
                                                   ccGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCgg                                                                                <  2:685670/140‑1 (MQ=255)
                                                   ccGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCgg                                                                                >  2:314040/1‑140 (MQ=255)
                                                           gCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGaaaaa                                                                        >  2:273426/1‑140 (MQ=255)
                                                           gCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGaaaaa                                                                        >  1:297658/1‑140 (MQ=255)
                                                                 tGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACgcagg                                                                  <  2:814430/140‑1 (MQ=255)
                                                                  gTTCCGGGA‑CGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACgcaggca                                                                <  2:903063/140‑1 (MQ=255)
                                                                   ttCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACgcaggca                                                                <  2:399451/140‑1 (MQ=255)
                                                                   ttCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACgcaggca                                                                <  2:805262/140‑1 (MQ=255)
                                                                    tCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACgcaggcag                                                               <  2:788363/140‑1 (MQ=255)
                                                                        ggATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACGCAGGCAGCGCt                                                           <  2:914453/140‑1 (MQ=255)
                                                                        ggATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACGCAGGCAGCGCt                                                           >  1:160031/1‑140 (MQ=255)
                                                                        ggATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCACGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACGCAGGCAGCGCt                                                           <  2:489250/140‑1 (MQ=255)
                                                                             gggTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACGCAGGCAGCGCTTTAGt                                                      >  1:1011405/1‑140 (MQ=255)
                                                                                    ttACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAACCGCAGGCAGCGGTTTAGTCTGATAg                                               <  2:31362/139‑1 (MQ=255)
                                                                                    cTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACGCAGGCAGCGCTTTAGTCTGATAg                                               <  2:976972/140‑1 (MQ=255)
                                                                                        cACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACGCAGGCAGCGCTTTAGTCTGATAGCCaa                                           >  2:854449/1‑140 (MQ=255)
                                                                                             ggCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACGCAGGCAGCGCTTTAGTCTGATAGc                                              >  1:713596/1‑132 (MQ=255)
                                                                                             ggCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACGCAGGCAGCGCTTTAGTCTGATAGCCAATTAAc                                      >  2:76240/1‑140 (MQ=255)
                                                                                             ggCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACGCAGGCAGCGCTTTAGTCTGATAGCCAATTAAc                                      >  1:248823/1‑140 (MQ=255)
                                                                                             ggCCACCGTTCAGACCGCTTTGCCATTACACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACGCAGGCAGCGCTTTAGTCTGATAGc                                              <  2:713596/132‑1 (MQ=255)
                                                                                                 aCCGTTCAGACCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACGCAGGCAGCGCTTTAGTCTGATAGCCAATTAACGGca                                  >  2:819403/1‑140 (MQ=255)
                                                                                                      tCAGACCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACGCAGGCAGCGCTTTAGTCTGATAGCCAATTAACGGCACACCg                             <  2:297658/140‑1 (MQ=255)
                                                                                                      tCAGACCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACGCAGGCAGCGCTTTAGTCTGATAGCCAATTAACGGCACACCg                             <  1:830208/140‑1 (MQ=255)
                                                                                                       cAGACCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACGCAGGCAGCGCTTTAGTCTGATAGCCAATTAACGGCACACCGa                            >  2:901018/1‑140 (MQ=255)
                                                                                                       cAGACCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACGCAGGCAGCGCTTTAGTCTGATAGCCAATTAACGGCACACCGa                            >  1:287604/1‑140 (MQ=255)
                                                                                                         gACCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACGCAGGCAGCGCTTTAGTCTGATAGCCAATTAACg                                     <  2:901468/129‑1 (MQ=255)
                                                                                                         gACCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACGCAGGCAGCGCTTTAGTCTGATAGCCAATTAACg                                     >  1:901468/1‑129 (MQ=255)
                                                                                                          aCCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACGCAGGCAGCGCTTTAGTCTGATAGCCAATTAACGGCACACCGAAgg                         <  2:343077/140‑1 (MQ=255)
                                                                                                          aCCGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACGCAGGCAGCGCTTTAGTCTGATAGCCAATTAACGGCACACCGAAgg                         <  1:471001/140‑1 (MQ=255)
                                                                                                            cGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACGCAGGCAGCGCTTTAGTCTGATAGCCAATTAACGGCACACCGAAgg                         >  2:288034/1‑138 (MQ=255)
                                                                                                            cGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACGCAGGCAGCGCTTTAGTCTGATAGCCAATTAACGGCACACCGAAgg                         <  1:288034/138‑1 (MQ=255)
                                                                                                            cGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACGCAGGCAGCGCTTTAGTCTGATAGCCAATTAACGGCACACCGAAGGtt                       >  2:276600/1‑140 (MQ=255)
                                                                                                            cGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACGCAGGCAGCGCTTTAGTCTGATAGCCAATTAACGGCACACCGAAGGtt                       <  2:1011405/140‑1 (MQ=255)
                                                                                                            cGCTTTGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACGCAGGCAGCGCTTTAGTCTGATAGCCAATTAACGGCACACCGAAGGtt                       >  1:1008633/1‑140 (MQ=255)
                                                                                                               tttGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACGCAGGCAGCGCTTTAGTCTGATAGCCAATTAACGGca                                  <  1:954305/126‑1 (MQ=255)
                                                                                                               tttGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACGCAGGCAGCGCTTTAGTCTGATAGCCAATTAACGGca                                  >  2:954305/1‑126 (MQ=255)
                                                                                                               tttGCCATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACGCAGGCAGCGCTTTAGTCTGATAGCCAATTAACGGCACACCGAAGGTTTCt                    >  2:776322/1‑140 (MQ=255)
                                                                                                                   ccATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACGCAGGCAGCGCTTTAGTCTGATAGCCAATTAACGGCACACCGAAGGTTTCTAAAt                <  2:983193/140‑1 (MQ=255)
                                                                                                                   ccATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACGCAGGCAGCGCTTTAGTCTGATAGCCAATTAACGGCACACCGAAGGTTTCTAAAt                <  1:854449/140‑1 (MQ=255)
                                                                                                                    cATTTCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACGCAGGCAGCGCTTTAGTCTGATAGCCAATTAACGGCACACCGAAGGTTTCTAAATa               <  2:1008633/140‑1 (MQ=255)
                                                                                                                      tttCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACGCAGGCAGCGCTTTAGTCTGATAGCCAATTAACGGCACACCGAAGGTTTCTAAATACt             <  1:76240/140‑1 (MQ=255)
                                                                                                                      ttgcaccacCATTGCGCGGGCAATTTCGCTGGCGCTGCCGAGACGAATGCTGACGTCAAACGGGCTGGTACAGCAGAAAAACGCAGGTAGCTCGATAGTCCGATAGCCAATTAACGGCACACCGAAGGTGTCTAAATACt             <  2:378045/137‑1 (MQ=255)
                                                                                                                       ttCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACGCAGGCAGCGCTTTAGTCTGATAGCCAATTAACGGCACACCGAAGGTTTCTAAATACTc            <  2:608221/140‑1 (MQ=255)
                                                                                                                       ttCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACGCAGGCAGCGCTTTAGTCTGATAGCCAATTAACGGCACACCGAAGGTTTCTAAATACTc            <  2:478439/140‑1 (MQ=255)
                                                                                                                        tCACCACCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACGCAGGCAGCGCTTTAGTCTGATAGCCAATTAACGGCACACCGAAGGTTCCTAAATACTCa           <  2:160031/140‑1 (MQ=255)
                                                                                                                         caccacCATTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACGCAGGCAGCGCTTTAGTCTGATAGCCAATTAACGGCACACCGAAGGTTTCTAAATACTCAg          <  1:273426/140‑1 (MQ=255)
                                                                                                                             accaTTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACGCAGGCAGCGCTTTAGTCTGATAGCCAATTAACGGCACACCGAAGGTTTCTAAATACTCAGTGGt      <  2:248823/140‑1 (MQ=255)
                                                                                                                             accaTTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACGCAGGCAGCGCTTTAGTCTGATAGCCAATTAACGGCACACCGAAGGTTTCTAAATACTCAGTGGt      <  2:868550/140‑1 (MQ=255)
                                                                                                                             accaTTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACGCAGGCAGCGCTTTAGTCTGATAGCCAATTAACGGCACACCGAAGGTTTCTAAATACTCAGTGGt      <  1:819403/140‑1 (MQ=255)
                                                                                                                             accaTTGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACGCAGGCAGCGCTTTAGTCTGATAGCCAATTAACGGCACACCGAAGGTTTCTAAATACTCAGTGGt      <  1:477930/140‑1 (MQ=255)
                                                                                                                                 ttGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACGCAGGCAGCGCTTTAGTCTGATAGCCAATTAACGGCACACCGAAGGTTTCTaaa                 <  2:671912/125‑1 (MQ=255)
                                                                                                                                 ttGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACGCAGGCAGCGCTTTAGTCTGATAGCCAATTAACGGCACACCGAAGGTTTCTaaa                 >  1:671912/1‑125 (MQ=255)
                                                                                                                                 ttGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACGCAGGCAGCGCTTTAGTCTGATAGCCAATTAACGGCACACCGAAGGTTTCTAAATACTCTGTGGTTAAt  >  1:353882/1‑140 (MQ=255)
                                                                                                                                 ttGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACGCAGGCAGCGCTTTAGTCTGATAGCCAATTAACGGCACACCGAAGGTTTCTAAATACTCAGTGGTTAAt  >  2:512921/1‑140 (MQ=255)
                                                                                                                                 ttGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACGCAGGCAGCGCTTTAGTCTGATAGCCAATTAACGGCACACCGAAGGTTTCTAAATACTCAGTGGTTAAt  <  1:276600/140‑1 (MQ=255)
                                                                                                                                 ttGCGCGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACGCAGACAGCGCTTTAGTCTGATAGCCAATTAACGGGACACCGAAGGTTTCTAAATACTCAGTGGTTAAt  >  2:811522/1‑140 (MQ=255)
                                                                                                                                     |                                                                                                                                       
GAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGATAGTGTGTTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCACGGGCAATTTCGCTGGCGCTGTCGAGACGAATGCTGACGTCAAACGGGCTGGTGCGGCAGAAAAACGCAGGCAGCGCTTTAGTCTGATAGCCAATTAACGGCACACCGAAGGTTTCTAAATACTCAGTGGTTAAT  >  NC_000913/2257584‑2257852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A