Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	155	60.3	2039550	99.3%	2025273	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,356,198	C→G	I226M (ATC→ATG)	glpC →	anaerobic glycerol‑3‑phosphate dehydrogenase subunit C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,356,198	0	C	G	100.0%	174.6 / NA	50	I226M (ATC→ATG)	glpC	anaerobic glycerol‑3‑phosphate dehydrogenase subunit C
Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (23/27); total (23/27)

AGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAA  >  NC_000913/2356060‑2356331

aGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAG‑AAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATGcg                                                                                                                                     >  2:78985/1‑140 (MQ=255)

    ttaattaaAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGGATGTAGAGTCGATGCGCGa                                                                                                                                  <  1:259825/140‑1 (MQ=255)

      aattaaAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATGCGCGAAg                                                                                                                                <  2:247163/140‑1 (MQ=255)

                cTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATGCGCGa                                                                                                                                  >  2:391688/1‑128 (MQ=255)

                cTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATGCGCGa                                                                                                                                  <  1:391688/128‑1 (MQ=255)

                cTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATGCGCGAAGCTGTGGGAGt                                                                                                                      >  1:270397/1‑140 (MQ=255)

                 tCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATGCGCGAAGCTGTGGGAGTa                                                                                                                     >  1:3177/1‑140 (MQ=255)

                   aaCGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATGCGCGAAGCTGTGGGAGTaaa                                                                                                                   <  1:100679/140‑1 (MQ=255)

                               aCCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATGCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGt                                                                                                       >  1:776581/1‑140 (MQ=255)

                               aCCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATGCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGt                                                                                                       >  1:444186/1‑140 (MQ=255)

                                    tgtACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTATGAATGTAGAGTCGATGCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTg                                                                                                  >  1:493059/1‑140 (MQ=255)

                                    tgtACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATGCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTg                                                                                                  >  2:289452/1‑140 (MQ=255)

                                      tACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATGCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGcc                                                                                                >  2:480232/1‑140 (MQ=255)

                                       aCAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATGCGCGAAGCTGTGGGGGTAAAAGGCATTCCGGTGATTGCCa                                                                                               <  1:785102/140‑1 (MQ=255)

                                       aCAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATGCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCa                                                                                               <  1:736713/140‑1 (MQ=255)

                                        cAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATGCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCAc                                                                                              >  1:169508/1‑140 (MQ=255)

                                              cTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATGCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCa                                                                                               >  1:158071/1‑133 (MQ=255)

                                              cTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATGCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCa                                                                                               <  2:158071/133‑1 (MQ=255)

                                              cTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATGCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCAcctcct                                                                                         <  1:394639/139‑1 (MQ=255)

                                              cTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATGCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCAcctcct                                                                                         >  2:394639/1‑139 (MQ=255)

                                              cTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATGCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACctcctc                                                                                        <  1:423665/140‑1 (MQ=255)

                                              cTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATGCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACctcctc                                                                                        >  2:708204/1‑140 (MQ=255)

                                              cTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATGCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACctcctc                                                                                        >  2:153632/1‑140 (MQ=255)

                                              cTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATGCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACctcctc                                                                                        >  2:113279/1‑140 (MQ=255)

                                              cTCAGCAAAG‑AAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATGCGCGAAGCTGTGGGAGTAAAAGGCGTTCCGGTGATTGCCACCTCCTCa                                                                                       <  1:78985/140‑1 (MQ=255)

                                               tCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATGCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCa                                                                                       <  2:444186/140‑1 (MQ=255)

                                               tCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATGCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCa                                                                                       <  2:838281/140‑1 (MQ=255)

                                                cAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATGCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCAcctcc                                                                                          <  2:177028/136‑1 (MQ=255)

                                                cAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATGCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCAcctcc                                                                                          >  1:177028/1‑136 (MQ=255)

                                                cAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATGCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCaa                                                                                      >  1:382655/1‑140 (MQ=255)

                                                cAGCAAAGAAAAATGCCGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATGCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCaa                                                                                      >  2:813941/1‑140 (MQ=255)

                                                   cAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATGCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCt                                                                                   >  2:870297/1‑140 (MQ=255)

                                                    aaaGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATGCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTg                                                                                  <  2:557650/140‑1 (MQ=255)

                                                    aaaGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATGCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTg                                                                                  <  1:289452/140‑1 (MQ=255)

                                                              gctgcGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATGCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGcc                                                                        <  1:870297/140‑1 (MQ=255)

                                                                  cggcgTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATGCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTgc                                                                    >  2:61770/1‑140 (MQ=255)

                                                                   ggcgTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATGCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTgcg                                                                   <  1:113279/140‑1 (MQ=255)

                                                                         ccGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATGCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGcgacga                                                             <  1:708204/140‑1 (MQ=255)

                                                                         ccGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATGCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGcgacga                                                             <  2:776581/140‑1 (MQ=255)

                                                                                 cGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATGCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCGCCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCgg                                                     <  2:3177/140‑1 (MQ=255)

                                                                                 cGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATGCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCgg                                                     <  1:61770/140‑1 (MQ=255)

                                                                                 cGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATGCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCgg                                                     <  1:153632/140‑1 (MQ=255)

                                                                                                        cACGCAAACAGGCAATTACGAATGTAGAGTCGATGCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGc                                               <  2:674450/123‑1 (MQ=255)

                                                                                                        cACGCAAACAGGCAATTACGAATGTAGAGTCGATGCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGc                                               >  1:674450/1‑123 (MQ=255)

                                                                                                         aCGCAAACAGGCAATTACGAATGTAGAGTCGATGCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAg                             >  1:930354/1‑140 (MQ=255)

                                                                                                           gCAAACAGGCAATTACGAATGTAGAGTCGATGCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGc                           <  2:382655/140‑1 (MQ=255)

                                                                                                               aCAGGCAATTACGAATGTAGAGTCGATGCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTgc                       <  2:270397/140‑1 (MQ=255)

                                                                                                                cAGGCAATTACGAATGTAGAGTCGATGCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTgcg                      <  2:666171/140‑1 (MQ=255)

                                                                                                                         aCGAATGTAGAGTCGATGCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCatat             >  1:247104/1‑140 (MQ=255)

                                                                                                                                   agTCGATGCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCa   >  1:964836/1‑140 (MQ=255)

                                                                                                                                    gTCGATGCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCaa  <  1:981795/140‑1 (MQ=255)

AGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAA  >  NC_000913/2356060‑2356331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A