Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	155	60.3	2039550	99.3%	2025273	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,437,329	T→C	T175A (ACG→GCG)	pdxB ←	erythronate‑4‑phosphate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,437,329	0	T	C	98.2%	183.6 / ‑4.1	54	T175A (ACG→GCG)	pdxB	erythronate‑4‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (34/19); total (35/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.67e-01

GGTCAGCAACGCAGTATTATCGACGACTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGTATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGGCGGATCGCAAAGTAAGGTTTTAATCCCTAACGCTTCCAGTCGCGCCTGCAATCG  >  NC_000913/2437195‑2437464

ggTCAGCAACGCAGTATTATCGACGACTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGCATGGa                                                                                                                                    <  2:953779/140‑1 (MQ=255)

 gTCAGCAACGCAGTATTATCGACGACTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGCATGGaa                                                                                                                                   >  2:220438/1‑140 (MQ=255)

 gTCAGCAACGCAGTATTATCGACGACTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGCATGGaa                                                                                                                                   >  2:216957/1‑140 (MQ=255)

       aaCGCAGTATTATCGACGACTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGCATGGAAAGTCAg                                                                                                                             >  2:56314/1‑140 (MQ=255)

           cAGTATTATCGACGACTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGCATGGAAAGTCAGAata                                                                                                                         >  1:737938/1‑140 (MQ=255)

                  atCGACGACTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGCATGGAAAGTCAGAATATCcgcgc                                                                                                                  <  2:574233/140‑1 (MQ=255)

                   tCGACGACTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGCATGGAAAGTCAGAATATCcgcgcg                                                                                                                 <  1:631158/140‑1 (MQ=255)

                    cgacgaCTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGCATGGAAAGTCAGAATATCCgcgcgc                                                                                                                <  2:737938/140‑1 (MQ=255)

                    cgacgaCTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGCATGGAAAGTCAGAATATCCgcgcgc                                                                                                                <  1:518471/140‑1 (MQ=255)

                     gacgacTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGCATGGAAAGTCAGAATATCCGCGCGCt                                                                                                               >  1:639906/1‑140 (MQ=255)

                        gacTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGTTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGCATGGAAAGTCAGAATATCCGCGCGCTGGa                                                                                                            >  2:511019/1‑140 (MQ=255)

                        gacTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGCATGGAAAGTCAGAATATCCGCGCGCTGGa                                                                                                            <  1:179591/140‑1 (MQ=255)

                        gacTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGCATGGAAAGTCAGAATATCCGCGCGCTGGa                                                                                                            >  2:674750/1‑140 (MQ=255)

                        gacTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGCATGGAAAGTCAGAATATCCGCGCGCTGGa                                                                                                            <  2:639906/140‑1 (MQ=255)

                          cTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGCATGGAAAGTCAGAATATCCGCGCGCTGGACt                                                                                                          >  2:601204/1‑140 (MQ=255)

                             cgcCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGCATGGAAAGTCAGAATATCcgcgc                                                                                                                  >  2:351242/1‑129 (MQ=255)

                             cgcCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGCATGGAAAGTCAGAATATCcgcgc                                                                                                                  <  1:351242/129‑1 (MQ=255)

                                         cGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGCATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCagcgag                                                                                           <  2:861903/140‑1 (MQ=255)

                                          gTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGCATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCagcgagc                                                                                          <  1:56314/140‑1 (MQ=255)

                                               tCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGCATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGaaa                                                                                     >  1:373788/1‑140 (MQ=255)

                                                   aaTCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGCATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCAc                                                                                 <  2:26132/140‑1 (MQ=255)

                                                      cGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGCATGGAAGGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCACCCt                                                                              <  1:1013497/140‑1 (MQ=255)

                                                       gCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGCATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCACCCTc                                                                             <  1:674750/140‑1 (MQ=255)

                                                                  cAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGCATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGt                                                                  >  1:515691/1‑140 (MQ=255)

                                                                       tACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGCATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGgcg                                                             <  2:373788/140‑1 (MQ=255)

                                                                          ggATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGCATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGa                                                          >  2:694122/1‑140 (MQ=255)

                                                                            aTCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGCATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAgg                                                        >  2:872215/1‑140 (MQ=255)

                                                                              cAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGCATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGGCg                                                      <  1:216957/140‑1 (MQ=255)

                                                                              cAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGCATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGGCg                                                      <  1:220438/140‑1 (MQ=255)

                                                                               aGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGCATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGGCgg                                                     >  1:381516/1‑140 (MQ=255)

                                                                                   tttCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGCATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGGCGGATCg                                                 >  1:319191/1‑140 (MQ=255)

                                                                                          cGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGCATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGGCGGATCGCAAAGTa                                          <  1:205720/140‑1 (MQ=255)

                                                                                           gCCAGATGTAGCGTTTTGTACGGACCATCTTCAAAGAGTGGCGCATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGGCGGATCGCAAAGTaa                                         >  1:710746/1‑140 (MQ=255)

                                                                                                           tGTACGGACCATCTTTAAAGAGTGGCGCATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGGCGGATCGCAAAGTAAGGTTTTAATCCCTAAc                         >  1:718852/1‑140 (MQ=255)

                                                                                                                ggACCATCTTTAAAGAGTGGCGCATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGGCGGATCGCAAAGTAGGGTTTTAATCCCTAACGCTTc                    >  2:926507/1‑140 (MQ=255)

                                                                                                                  aCCATCTTTAAAGAGTGGCGCATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGGCGGATCGCAAAGTAAGGTTTTAATCCCTAACGCTTCCa                  >  2:85976/1‑140 (MQ=255)

                                                                                                                       cTTTAAAGAGTGGCGCATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGGCGGATCGCAAAGTAAGGTTTTAATCCCTAACGCTTCCAGTcgc             >  1:226653/1‑140 (MQ=255)

                                                                                                                       cTTTAAAGAGTGGCGCATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGGCGGATCACAAAGTAAGGTTTTAATCCCTAACGCTTCCAGTcgc             >  2:570966/1‑140 (MQ=255)

                                                                                                                            aaGAGTGGCGTATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGGCGGATCGCAAAGTAAGGTTTTAATCCCTAACGCTTCCAGTCGCGCCTg        >  2:523953/1‑140 (MQ=255)

                                                                                                                                 tGGCGCATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCACCCTGATCCCCACGGTCGGCGCGAGGCGGATCGCAAAGTAAGGTTTTAATCCCTAACGCTTCCAGTCGCGCCTGCAATc   >  2:895420/1‑140 (MQ=255)

                                                                                                                                 tGGCGCATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGGCGGATCGCAAAGTAAGGTTTTAATCCCTAACGCTTCCAGTCGCGCCTGCAATc   >  2:875421/1‑140 (MQ=255)

                                                                                                                                 tGGCGCATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGGCGGATCGCAAAGTAAGGTTTTAATCCCTAACGCTTCCAGTCGCGCCTGCAATc   >  1:539798/1‑140 (MQ=255)

                                                                                                                                 tGGCGCATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGGCGGATCGCAAAGTAAGGTTTTAATCCCTAACGCTTCCAGTCGCGCCTGCAATc   >  2:928898/1‑140 (MQ=255)

                                                                                                                                 tGGCGCATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGGCGGATCGCAAAGTAAGGTTTTAATCCCTAACGCTTCCAGTCGCGCCTGCAATc   >  1:821820/1‑140 (MQ=255)

                                                                                                                                 tGGCGCATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGGCGGATCGCAAAGTAAGGTTTTAATCCCTAACGCTTCCAGTCGCGCCTGCAATc   <  2:753355/140‑1 (MQ=255)

                                                                                                                                 tGGCGCATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGGCGGATCGCAAAGTAAGGTTTTAATCCCTAACGCTTCCAGTCGCGCCTGCAATc   >  2:730133/1‑140 (MQ=255)

                                                                                                                                 tGGCGCATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGGCGGATCGCAAAGTAAGGTTTTAATCCCTAACGCTTCCAGTCGCGCCTGCAATc   >  2:645610/1‑140 (MQ=255)

                                                                                                                                 tGGCGCATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGGCGGATCGCAAAGTAAGGTTTTAATCCCTAACGCTTCCAGTCGCGCCTGCAATc   >  1:25213/1‑140 (MQ=255)

                                                                                                                                 tGGCGCATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGGCGGATCGCAAAGTAAGGTTTTAATCCCTAACGCTTCCAGTCGCGCCTGCAATc   >  1:378467/1‑140 (MQ=255)

                                                                                                                                 tGGCGCATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGGCGGATCGCAAAGTAAGGTTTTAATCCCTAACGCTTCCAGTCGCGCCTGCAATc   >  1:893428/1‑140 (MQ=255)

                                                                                                                                 tGGCGCATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGGCGGATCGCAAAGTAAGGTTTTAATCCCTAACGCTTCCAGTCGCGCCTGCAATc   >  1:849688/1‑140 (MQ=255)

                                                                                                                                 tGGCGCATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGGCGGATCGCAAAGTAAGGTTTTAATCCCTAACGCTTCCAGTCGCGCCTACAATc   >  2:900912/1‑140 (MQ=255)

                                                                                                                                  ggCGCATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGGCGGATCGCAAAGTAAGGTTTTAATCCCTAACGCTTCCAGTCGCGCCTGCAATCg  >  2:530120/1‑140 (MQ=255)

                                                                                                                                  ggCGCATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGGCGGATCGCAAAGTAAGGTTTTAATCCCTAACGCTTCCAGTCGCGCCTGCAATCg  <  2:319191/140‑1 (MQ=255)

GGTCAGCAACGCAGTATTATCGACGACTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGTATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGGCGGATCGCAAAGTAAGGTTTTAATCCCTAACGCTTCCAGTCGCGCCTGCAATCG  >  NC_000913/2437195‑2437464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A