Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F28 I1 R1
|
159 |
82.1 |
2830106 |
99.3% |
2810295 |
139.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,437,568 |
A→G |
V95A (GTG→GCG) |
pdxB ← |
erythronate‑4‑phosphate dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,437,568 | 0 | A | G | 96.5%
| 96.5
/ ‑4.0
| 29 | V95A (GTG→GCG) | pdxB | erythronate‑4‑phosphate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (19/9); minor base T (0/1); total (19/10) |
| Fisher's exact test for biased strand distribution p-value = 3.45e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 |
CTAACGCTTCCAGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGATCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATCAGCAGGGAGGAGAAAACATATTCCACCACCGCAATCGCATTACAGCCAGGTGCAGCGGAAAAACCAATTCCCGCCTGCTTCAACCATGCTTCATCGACATGGTCGGTCCCCGCAGTGGCAGTGCCAACAAATTTAATGGGTTTTCCTGCCAGCA > NC_000913/2437437‑2437694
|
ctaccccTTCCAGTCGCGCCTACAACCGACGTCCAACGTTCCCAACCCCCACGACCCCCCCCGTAGGGTCGTACAGTGAAAATCCATCGCGTCCGGCAAGCATCAGCAGGGAGGAGAAAACATATCCCACCGCCGCAATc < 2:1175178/134‑1 (MQ=255)
tAACGCTTCCAGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGATCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATCAGCAGGGAGGAGAAAACATATTCCACCGCCGCAATCg > 1:1175178/1‑140 (MQ=255)
tAACGCTTCCAGCCGCGCCTGCAATCGACGACCAACGTTACCACCCCCCACGACCCCCACCGTACGGTCGTACAGTAAAAACCCATCGCGTCCGGCAAGCATCAGCAGGGAGGAAAAAACATATTCCACCGCCGCAATCg < 2:677602/140‑1 (MQ=255)
ccGCTTCCAGCCGCGCCTGCAATCGACGTCCAACGTTCCCACCCCCCACGACCCCCACCGTACGGTCGTACAGTGAAAACCCATCGCGTTCGGCAAGCATCAGCAGGGAGGAGAAAACATATTCCACCGCCGCAATCGCa < 2:412746/139‑1 (MQ=255)
cTTCCAGTCGCGCCTGCAATCGACGTCCAACGTTACCACCGCCCACGATCCCCCCCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATCAGCAGGGAGGAGAAAACATATTCCACCGCCGCAATCGCATTa < 1:59846/140‑1 (MQ=255)
cTTCCAGTCGCGCCTGCAATCGACGCTCACCTTTACCAACGCCCACGACCCCCCCCGTAGGGTCGTACAGTGAAAATCCATCGCGTGCGGCAAGCATCAGCAGGGCGGAGAAAACATATTCCACCGCCGCAATCGCATTa < 2:1252934/140‑1 (MQ=255)
ccgcccccgccccCCCCCGTGGGGTCGAACAGTAAAAACCCACCGCGTCGGGAAAGCACCAGCGGGGGGGAAAAAACATATCCCCCCGCCGCAATCGCATTACAGCCAGGTGCAGCGGAAAAACCAATTCCCGCCTGCtt < 2:471124/139‑1 (MQ=255)
cgccccccccCGTCGGGTCGTAAAGAAAAAACCCACCGCGTCCGAAAACAACCGGGGGGGGGAAAAAAAAATTTCCCCCCGCCGCAATCGCATTACAGCCAGGTGCAGCGGAAAAACCAATTCCCGCCTGCTTCAACCAt < 2:18926/136‑1 (MQ=255)
gATCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATCAGCAGGGAGGAGAAAACATATTCCACCTCCGCAATCGCATTACAGCCAGGTGCAGCGGAAAAACCAATTCCCGCCTGCTTCAACCATg < 1:1351306/140‑1 (MQ=255)
aTCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATCAGCAGGGAGGAGAAAACATATTCCACCGCCGCAATCGCATTACAGCCAGGTGCAGCGGAAAAACCAATTCCCGCCTGCTTCAACCATGc > 1:1184050/1‑140 (MQ=255)
tCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATCAGCAGGGAGGAGAAAACATATTCCACCGCCGCAATCGCATTACAGCCAGGTGCAGCGGAAAAACCAATTCCCGCCTGCTTCAACCCTgtc > 1:1412721/1‑138 (MQ=255)
cccACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATCAGCAGGGAGGAGAAAACATATTCCACCGCCGCAATCGCATTACAGCCAGGTGCAGCGGAAAAACCAATTCCCGCCTGCTTCAACCATGCTTc > 1:397818/1‑140 (MQ=255)
gTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATCAGCAGGGAGGAGAAAACATATTCCACCGCCGCAATCGCATTACAGCCAGGTGCAGCGGAAAAACCAATTCCCGCCTGCTTCAACCATGCTTCATCGAc > 1:108079/1‑140 (MQ=255)
gTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATCAGCAGGGAGGAGAAAACATATTCCACCGCCGCAATCGCATTACAGCCAGGTGCAGCGGAAAAACCAATTCCCGCCTGCTTCAACCATGCTTCATCGAc > 1:347399/1‑140 (MQ=255)
tACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATCAGCAGAGAGGAGAAAACATATTCCACCGCCGCAATCGCATTACAGCCAGGTGCAGCGGAAAAACCAATTCCCGCCTGCTTCAACCATGCTTCATCGACa > 1:75901/1‑140 (MQ=255)
agaaaaaaCCCACCGCTTCGGGCAACCATCGGAGGGGAGGAAAAAACATTTCCCCCCGCCGCAATCGCATTACAGCCAGGTGCAGCGGAAAAACCAATTCCCGCCTGCTTCAACCATGCTTCATCGACATGGTCGGTccc < 2:1082474/136‑1 (MQ=255)
aaaaTCCATCGCGTTCGGCAAGCATCAGCAGGGAGGAGAAAACATATTCCACCGCCGCAATCGCATTACAGCCAGGTGCAGCGGAAAAACCAATTCCCGCCTGCTTCAACCATGCTTCATCGACATGGTCGGTCCCCgca > 1:144135/1‑140 (MQ=255)
aaaaTCCATCGCGTTCGGCAAGCATCAGCAGGGAGGAGAAAACATATTCCACCGCCGCAATCGCATTACAGCCAGGTGCAGCGGAAAAACCAATTCCCGCCTGCTTCAACCATGCTTCATCGACATGGTCGGTCCCCgca > 2:754056/1‑140 (MQ=255)
aaaaTCCATCGCGTTCGGCAAGCATCAGCAGGGAGGAGAAAACATATTCCACCGCCGCAATCGCATTACAGCCAGGTGCAGCGGAAAAACCAATTCCCGCCTGCTTCAACCATGCTTCATCGACATGGTCGGTCCCCgca > 2:843713/1‑140 (MQ=255)
aaaaTCCATCGCGTTCGGCAAGCATCAGCAGGGAGGAGAAAACATATTCCACCGCCGCAATCGCATTACAGCCAGGGGCAGCGGAAAAACCAATTCCCGCCTGCTTCAACCATGCTTCATCGACATGGTCGGTCCACgca > 2:495660/1‑140 (MQ=255)
aaTCCATCGCGTTCGGCAAGCATCAGCAGGGAGGAGAAAACATATTCCACCGCCGCAATCGCATTACAGCCAGGTGCAGCGGAAAAACCAATTCCCGCCTGCTTCAACCATGCTTCATCGACATGGTCGGTCCCCgcagt > 1:1213432/1‑140 (MQ=255)
aTCCATCGCGTTCGGCAAGCATCAGCAGGGAGGAGAAAACATATTCCACCGCCGCAATCGCATTACAGCCAGGTGCAGCGGAAAAACCAATTCCCGCCTGCTTCAACCATGCTTCATCGACATGGTCGGTCCCCgcagtg > 2:170685/1‑140 (MQ=255)
gttgcGGAAAGCACCAGCGGGGAGGAAAAAACATATCCCCCCGCCGCAATCGCATTACAGCCAGGTGCAGCGGAAAAACCAATTCCCGCCTGCTTCAACCATGCTTCATCGACATGGTCGGTCCCCGCAGTGGCAGTGcc < 2:144135/136‑1 (MQ=255)
ttCGGCAAGCATCAGCAGGGAGGAGAAAACATATTCCACCGCCGCAATCGCATTACAGCCAGGTGCAGCGGAAAAACCAATTCCCGCCTGCTTCAACCATGCTTCATCGACATGGTCGGTCCCCGCAGTGGCAGTGCcaa > 2:1280274/1‑140 (MQ=255)
ttCGGCAAGCATCAGCAGGGAGGAGAAAACATATTCCACCGCCGCAATCGCATTACAGCCAGGTGCAGCGGAAAAACCAATTCCCGCCTGCTTCAACCATGCTTCATCGACATGGTCGGTCCCCGCAGTGGCAGTGCcaa > 2:394731/1‑140 (MQ=255)
gggaaaGCAGCAGAGGGAAGGAAAAACCATTTTCCCCCGCCGCAATCGCATTACAGCCAGGTGCAGCGGAAAAACCAATTCCCGCCTGCTTCAACCATGCTTCATCGACATGGTCGGTCCCCGCAGTGGCAGTGCcaaca < 1:63369/136‑1 (MQ=255)
ggaggaGAAAACATATTCCACCGCCGCAATCGCATTACAGCCAGGTGCAGCGGAAAAACCAATTCCCGCCTGCTTCAACCATGCTTCATCGACATGGTCGGTCCCCGCAGTGGCAGTGCCAACAAATTTAATGGGTTTTc > 1:107140/1‑140 (MQ=255)
aggagAAAACATATTCCACCGCCGCAATCGCATTACAGCCAGGTGCAGCGGAAAAACCAATTCCCGCCTGCTTCAACCATGCTTCATCGACATGGTCGGTCCCCGCAGTGGCAGTGCCAACAAATTTAATGGGTTTTCCt > 2:451441/1‑140 (MQ=255)
gagaAAACATATTCCACCGCCGCAATCGCATTACAGCCAGGTGCAGCGGAAAAACCAATTCCCGCCTGCTTCAACCATGCTTCATCGACATGGTCGGTCCCCGCAGTGGCAGTGCCAACAAATTTAATGGGTTTTCCTGc > 1:183765/1‑140 (MQ=255)
agaAAACATATTCCACCGCCGCAATCGCATTACAGCCAGGTGCAGCGGAAAAACCAATTCCCGCCTGCTTCAACCATGCTTCATCGACATGGTCGGTCCCCGCAGTGGCAGTGCCAACAAATTTAATGGGTTTTCCTGcc > 2:378337/1‑140 (MQ=255)
aaCATATTCCACCGCCGCAATCGCATTACAGCCAGGTGCAGCGGAAAAACCAATTCCCGCCTGCTTCAACCATGCTTCATCGACATGGTCGGTCCCCGCAGTGGCAGTGCCAACAAATTTAATGGGTTTTCCTGCCAGCa < 1:754056/140‑1 (MQ=255)
|
CTAACGCTTCCAGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGATCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATCAGCAGGGAGGAGAAAACATATTCCACCACCGCAATCGCATTACAGCCAGGTGCAGCGGAAAAACCAATTCCCGCCTGCTTCAACCATGCTTCATCGACATGGTCGGTCCCCGCAGTGGCAGTGCCAACAAATTTAATGGGTTTTCCTGCCAGCA > NC_000913/2437437‑2437694
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
