Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	155	60.3	2039550	99.3%	2025273	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,496,557	T→C	Y305H (TAC→CAC)	lpxP →	palmitoleoyl acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,496,557	0	T	C	100.0%	106.2 / NA	31	Y305H (TAC→CAC)	lpxP	palmitoleoyl acyltransferase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (12/19); total (12/19)

GCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGAATATCTAATTATTACCGTCAGAAATTAAA  >  NC_000913/2496423‑2496684

gCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGCACAtt                                                                                                                            >  1:190425/1‑140 (MQ=255)

  tACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGCACATTTa                                                                                                                          >  2:16590/1‑140 (MQ=255)

              gAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGCACATTTAACAAACTGctct                                                                                                              <  1:492536/140‑1 (MQ=255)

                  aTCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGCACATTTAACAAACTGCTCTTAct                                                                                                          <  1:183561/140‑1 (MQ=255)

                           cTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGCACATTTAACAAACTGCTCTTACTCTTTCcctc                                                                                                 <  2:242121/140‑1 (MQ=255)

                                 atatGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGCACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCaaa                                                                                           <  1:302968/140‑1 (MQ=255)

                                 atatGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGCACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCaaa                                                                                           <  1:338732/140‑1 (MQ=255)

                                 atatGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGCACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCaaa                                                                                           <  2:179298/140‑1 (MQ=255)

                                  tatGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGCACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAAt                                                                                          <  2:758072/140‑1 (MQ=255)

                                     gAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGCACATTTAACGAACTGCTCTTACTCTTTCCCTCCTCAAATCgg                                                                                       <  1:502774/140‑1 (MQ=255)

                                     gAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGCACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCgg                                                                                       <  2:641850/140‑1 (MQ=255)

                                      aaCAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGCACATTTAACAAACTGCTCTTACTCTTTTCCTCCTCAAATCGGc                                                                                      <  1:213170/140‑1 (MQ=255)

                                          aGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGCACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGCCata                                                                                  >  2:719214/1‑140 (MQ=255)

                                            aTTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGCACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGCCataat                                                                                >  1:202218/1‑140 (MQ=255)

                                              tATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGCACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCg                                                                                        <  1:507687/130‑1 (MQ=255)

                                              tATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGCACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCg                                                                                        >  2:507687/1‑130 (MQ=255)

                                                     aaaGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGCACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGCCATAATAACCTCATg                                                                       <  1:1005752/140‑1 (MQ=255)

                                                      aaGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGCACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGCCATAATAACCTCATgg                                                                      >  2:679252/1‑140 (MQ=255)

                                                          gaTCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGCACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGCCataa                                                                                 <  2:1015241/125‑1 (MQ=255)

                                                          gaTCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGCACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGCCataa                                                                                 >  1:1015241/1‑125 (MQ=255)

                                                                          gAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGCACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTa                                                  <  1:931921/140‑1 (MQ=255)

                                                                             cAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGCACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAgg                                               >  2:655380/1‑140 (MQ=255)

                                                                                          tCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGCACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGc                                  >  2:933857/1‑140 (MQ=255)

                                                                                             aCCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGCACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGa                               <  1:933857/140‑1 (MQ=255)

                                                                                             aCCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGCACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGa                               <  1:655380/140‑1 (MQ=255)

                                                                                             aCCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGCACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGa                               >  1:306219/1‑140 (MQ=255)

                                                                                                  cGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGCACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGAATATc                          >  2:647666/1‑140 (MQ=255)

                                                                                                    cTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGCACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGa                               >  2:450140/1‑133 (MQ=255)

                                                                                                    cTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGCACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGa                               <  1:450140/133‑1 (MQ=255)

                                                                                                                  cGGTGGGAGAATCGTCGTTGCACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGAATATCTAATTATTACCGTCAg          <  1:679252/140‑1 (MQ=255)

                                                                                                                          gaATCGTCGTTGCACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGAATATCTAATTATTACCGTCAGAAATTaaa  <  2:306219/140‑1 (MQ=255)

GCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGAATATCTAATTATTACCGTCAGAAATTAAA  >  NC_000913/2496423‑2496684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A