Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F28 I1 R1 155 60.3 2039550 99.3% 2025273 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,764,942 T→C D71G (GAC→GGC)  abpA ← CP4‑57 prophage; anti‑bacteriophage protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,764,9420TC97.7% 298.7 / ‑2.6 88D71G (GAC→GGC) abpACP4‑57 prophage; anti‑bacteriophage protein
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base C (47/39);  total (47/41)
Fisher's exact test for biased strand distribution p-value = 2.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

CTGTGACGATGGAGTACCTACGTCCAAGGGACAGGTCAGACTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGTCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTATCCATCAGATACAATGTCGATATCGTCTGTGACCTCGCAGCGCACACTGCGGATAGTCTTGTCACGAAGCATTCGGGTGAC  >  NC_000913/2764823‑2765078
                                                                                                                       |                                                                                                                                        
ctGTGACGATGGAGTACCTACGTCCAAGGGACAGGTCAGACTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGtt                                                                                                                      >  1:14165/1‑140 (MQ=255)
    gACGATGGAGTACCTACGTCCAAGGGACAGGTCAGACTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGc                                                                                                                  <  2:893019/140‑1 (MQ=255)
       gATGGAGTACCTACGTCCAAGGGACAGGTCAGACTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCgg                                                                                                               <  1:75767/140‑1 (MQ=255)
       gATGGAGTACCTACGTCCAAGGGACAGGTCAGACTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCgg                                                                                                               <  1:165299/140‑1 (MQ=255)
        aTGGAGTACCTACGTCCAAGGGACAGGTCAGACTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGt                                                                                                              >  2:704496/1‑140 (MQ=255)
           gAGTACCTACGTCCAAGGGACAGGTCAGACTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCt                                                                                                           >  2:113163/1‑140 (MQ=255)
             gTACCTACGTCCAAGGGACAGGTCAGACTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCttt                                                                                                         >  1:278839/1‑140 (MQ=255)
                 cTACGTCCAAGGGACAGGTCAGACTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTAc                                                                                                     <  1:422439/140‑1 (MQ=255)
                 cTACGTCCAAGGGACAGGTCAGACTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTAc                                                                                                     >  1:22927/1‑140 (MQ=255)
                     gTCCAAGGGACAGGTCAGACTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGa                                                                                                 >  2:426266/1‑140 (MQ=255)
                      tCCAAGGGACAGGTCAGACTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGaa                                                                                                >  1:894153/1‑140 (MQ=255)
                      tCCAAGGGACAGGTCAGACTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGaa                                                                                                <  2:749935/140‑1 (MQ=255)
                      tCCAAGGGACAGGTCAGACTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGaa                                                                                                >  2:511418/1‑140 (MQ=255)
                      tCCAAGGGACAGGTCAGACTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGaa                                                                                                >  1:307838/1‑140 (MQ=255)
                      tCCAAGGGACAGGTCAGACTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGaa                                                                                                >  1:907228/1‑140 (MQ=255)
                      tCCAAGGGACAGGTCAGACTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGaa                                                                                                >  2:436809/1‑140 (MQ=255)
                       ccAAGGGACAGGTCAGACTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAAc                                                                                               <  2:22927/140‑1 (MQ=255)
                        cAAGGGACAGGTCAGACTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACg                                                                                              >  1:617510/1‑140 (MQ=255)
                          aGGGACAGGTCAGACTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGaa                                                                                                <  1:993815/136‑1 (MQ=255)
                          aGGGACAGGTCAGACTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGaa                                                                                                >  2:993815/1‑136 (MQ=255)
                          aGGGACAGGTCAGACTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGaa                                                                                            >  2:101307/1‑140 (MQ=255)
                          aGGGACAGGTCAGACTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGaa                                                                                            >  1:632377/1‑140 (MQ=255)
                          aGGGACAGGTCAGACTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGaa                                                                                            >  1:68438/1‑140 (MQ=255)
                          aGGGACAGGTCAGACTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTCTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGaa                                                                                            <  2:947952/140‑1 (MQ=255)
                           gggACAGGTCAGACTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAg                                                                                           <  2:307838/140‑1 (MQ=255)
                              aCAGGTCAGACTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGTCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCg                                                                                        <  1:539262/140‑1 (MQ=255)
                              aCAGGTCAGACTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCg                                                                                        <  1:68631/140‑1 (MQ=255)
                                aGGTCAGACTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGAt                                                                                      >  1:389232/1‑140 (MQ=255)
                                aGGTCAGACTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGAt                                                                                      >  1:585280/1‑140 (MQ=255)
                                aGGTCAGACTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGAt                                                                                      >  1:208536/1‑140 (MQ=255)
                                aGGTCAGACTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGAt                                                                                      <  1:71546/140‑1 (MQ=255)
                                aGGTCAGACTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGAt                                                                                      <  1:682207/140‑1 (MQ=255)
                                aGGTCAGACTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACC‑TTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGa                                                                                             >  1:1010416/1‑132 (MQ=255)
                                aGGTCAGACTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACC‑TTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGa                                                                                             <  2:1010416/132‑1 (MQ=255)
                                 ggTCAGACTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGaa                                                                                            <  1:115355/133‑1 (MQ=255)
                                 ggTCAGACTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGaa                                                                                            >  2:115355/1‑133 (MQ=255)
                                 ggTCAGACTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATg                                                                                     >  2:180496/1‑140 (MQ=255)
                                  gTCAGACTCACACTGCATTGATTTGTGCAAAATTGAGCTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGt                                                                                    >  2:580964/1‑140 (MQ=255)
                                   tCAGACTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTa                                                                                   <  1:704496/140‑1 (MQ=255)
                                    cAGACTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGTCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTAt                                                                                  <  1:186817/140‑1 (MQ=255)
                                    cAGACTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTAt                                                                                  <  2:585280/140‑1 (MQ=255)
                                    cAGACTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTAt                                                                                  <  2:44283/140‑1 (MQ=255)
                                    cAGACTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTAt                                                                                  <  2:208536/140‑1 (MQ=255)
                                    cAGACTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTAt                                                                                  <  1:768032/140‑1 (MQ=255)
                                    cAGACTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTAt                                                                                  <  1:113163/140‑1 (MQ=255)
                                    cAGACTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTAt                                                                                  <  1:65863/140‑1 (MQ=255)
                                       aCTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTAt                                                                                  <  1:694668/137‑1 (MQ=255)
                                       aCTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTAt                                                                                  >  2:694668/1‑137 (MQ=255)
                                       aCTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTAt                                                                                  <  1:95682/137‑1 (MQ=255)
                                       aCTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTAt                                                                                  >  2:95682/1‑137 (MQ=255)
                                           acacTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAg                                                                                           >  2:251162/1‑124 (MQ=255)
                                           acacTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAg                                                                                           <  1:251162/124‑1 (MQ=255)
                                           acacTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTAt                                                                                  <  2:401816/133‑1 (MQ=255)
                                           acacTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTAt                                                                                  >  1:401816/1‑133 (MQ=255)
                                            cacTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTATCCATCAGa                                                                          >  2:905339/1‑140 (MQ=255)
                                            cacTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTATCCATCAGa                                                                          >  1:105620/1‑140 (MQ=255)
                                            cacTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTATCCATCAGa                                                                          >  2:632972/1‑140 (MQ=255)
                                            cacTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTATCCATCAGa                                                                          >  1:312131/1‑140 (MQ=255)
                                            cacTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTATCCATCAGa                                                                          >  1:984696/1‑140 (MQ=255)
                                                 cATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTATCCATCAGATACaa                                                                     >  1:524156/1‑140 (MQ=255)
                                                 cATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTATCCATCAGATACaa                                                                     >  2:560674/1‑140 (MQ=255)
                                                     gATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTATCCATCAGATACAATGTc                                                                 >  2:991399/1‑140 (MQ=255)
                                                         tgtgCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTATCCATCAGATACAATGTCGata                                                             <  2:14165/140‑1 (MQ=255)
                                                         tgtgCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTATCCATCAGATACAATGTCGata                                                             <  2:524156/140‑1 (MQ=255)
                                                           tgCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTATCCATCAGATACAATGTCGATATc                                                           >  1:422688/1‑140 (MQ=255)
                                                                     aaCTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTATCCATCAGATACAATGTCGATATCGTCTGTGAcc                                                 <  2:907228/140‑1 (MQ=255)
                                                                          cAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTATCCATCAGATACAATGTCGata                                                             <  1:431439/123‑1 (MQ=255)
                                                                          cAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTATCCATCAGATACAATGTCGata                                                             >  2:431439/1‑123 (MQ=255)
                                                                          cAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTATCCATCAGATACAATGTCGATATCGTCTGTGACCTCGCa                                            <  2:105620/140‑1 (MQ=255)
                                                                               gATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTATCCATCAGATACAATGTCGATATCGTCTGTGACCTCGCAGCGca                                       >  2:354692/1‑140 (MQ=255)
                                                                               gATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTATCCATCAGATACAATGTCGATATCGTCTGTGACCTCGCAGCGca                                       >  2:142278/1‑140 (MQ=255)
                                                                                 tCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTATCCATCAGATACAATGTCGATATCGTCTGTGACCTCGCAGCGcaca                                     <  2:632377/140‑1 (MQ=255)
                                                                                 tCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTATCCATCAGATACAATGTCGATATCGTCTGTGACCTCGCAGCGcaca                                     <  1:493241/140‑1 (MQ=255)
                                                                                  cGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTATCCATCAGATACAATGTCGATATCGTCTGTGACCTCGCAGCGCacac                                    >  1:241139/1‑140 (MQ=255)
                                                                                   gTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTATCCATCAGATACAATGTCGATATCGTCTGTGACCTCGCAGCGCACACt                                   <  1:560674/140‑1 (MQ=255)
                                                                                       ttttATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTATCCATCAGATACAATGTCGATATCGTCTGTGACCTCGCAGCGCACACTGCgg                               >  1:34308/1‑140 (MQ=255)
                                                                                        tttATCAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTATCCATCAGATACAATGTCGATATCGTCTGTGACCTCGCAGCGCACACTGCGGa                              <  2:34308/140‑1 (MQ=255)
                                                                                             cAGCACCTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTATCCATCAGATACAATGTCGATATCGTCTGTGACCTCGCAGCGCACACTGCGGATAGTc                         <  2:312131/140‑1 (MQ=255)
                                                                                                   cTTTTGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTATCCATCAGATACAATGTCGATATCGTCTGTGACCTCGCAGCGCACACTGCGGATAGTCTTGTCa                   >  2:644171/1‑140 (MQ=255)
                                                                                                     tttGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTATCCATCAGATACAATGTCGATATCGTCTGTGACCTCGCAGCGCACACTGCGGATAGTCTTGTCACg                 >  1:30752/1‑140 (MQ=255)
                                                                                                      ttGAATTCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTATCCATCAGATACAATGTCGATATCGTCTGTGACCTCGCAGCGCACACTGCGGATAGTCTTGTCACGa                >  1:954722/1‑140 (MQ=255)
                                                                                                           ttCTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTATCCATCAGATACAATGTCGATATCGTCTGTGACCTCGCAGCGCACACTGCGGATAGTCTTGTCACGAAGCAt           <  1:632972/140‑1 (MQ=255)
                                                                                                             cTGAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTATCCATCAGATACAATGTCGATATCGTCTGTGACCTCGCAGCGCACACTGCGGATAGTCTTGTCACGAAGCATTc         >  2:89620/1‑140 (MQ=255)
                                                                                                               gAACGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTATCCATCAGATACAATGTCGATATCGTCTGTGACCTCGCAGCGCACACTGCGGATAGTCTTGTCACGAAGCATTCgg       <  1:436809/140‑1 (MQ=255)
                                                                                                                aaCGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTATCCATCAGATACAATGTCGATATCGTCTGTGACCTCGCAGCGCACACTGCGGATAGTCTTGTCACGAAGCATTTggg      >  2:141753/1‑140 (MQ=255)
                                                                                                                aaCGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTATCCATCAGATACAATGTCGATATCGTCTGTGACCTCGCAGCGCACACTGCGGATAGTCTTGTCACGAAGCATTTggg      >  2:995664/1‑140 (MQ=255)
                                                                                                                 aCGATGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTATCCATCAGATACAATGTCGATATCGTCTGTGACCTCGCAGCGCACACTGCGGATAGTCTTGTCACGAAGCATTCGGGt     <  2:894153/140‑1 (MQ=255)
                                                                                                                    aTGCCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTATCCATCAGATACAATGTCGATATCGTCTGTGACCTCGCAGCGCACACTGCGGATAGTCTTGTCACGAAGCATTCGGGTGAc  <  2:617510/140‑1 (MQ=255)
                                                                                                                       |                                                                                                                                        
CTGTGACGATGGAGTACCTACGTCCAAGGGACAGGTCAGACTCACACTGCATTGATTTGTGCAAAATTGAACTGCAGGGGATCGTCTTTTTATCAGCACCTTTTGAATTCTGAACGATGTCCGAAATGTTCCAACGTGTTTTGCCGGTGCTTTTTACCTGAACGAAGTCGATGTATCCATCAGATACAATGTCGATATCGTCTGTGACCTCGCAGCGCACACTGCGGATAGTCTTGTCACGAAGCATTCGGGTGAC  >  NC_000913/2764823‑2765078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A