Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	155	60.3	2039550	99.3%	2025273	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,790,138	G→A	G53R (GGG→AGG)	lhgO →	L‑2‑hydroxyglutarate oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,790,138	0	G	A	100.0%	137.2 / NA	39	G53R (GGG→AGG)	lhgO	L‑2‑hydroxyglutarate oxidase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (21/18); total (21/18)

ATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTC  >  NC_000913/2790015‑2790265

atcGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCAGGGTCTATTACACGcc                                                                                                                 <  2:380218/140‑1 (MQ=255)

         tCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCAATGCCAGGGTCTATTACACGCCCGGCAGCCt                                                                                                        >  2:437226/1‑140 (MQ=255)

          cGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCAATGCCAGGGTCTATTACACGCCCGGCAGCCTg                                                                                                       <  1:437226/140‑1 (MQ=255)

              cGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCAGGGTCTATTACACGCCCgg                                                                                                              >  1:188171/1‑129 (MQ=255)

              cGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCAGGGTCTATTACACGCCCgg                                                                                                              <  2:188171/129‑1 (MQ=255)

                 cATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCAGGGTCTATTACACGCCCGGCAGCCTGAAGgcgc                                                                                                >  1:980112/1‑140 (MQ=255)

                                     aTCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCAGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGcc                                                                                       <  2:967350/129‑1 (MQ=255)

                                     aTCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCAGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGcc                                                                                       >  1:967350/1‑129 (MQ=255)

                                     aTCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCAGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAAc                                                                            >  2:789499/1‑140 (MQ=255)

                                     aTCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCAGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAAc                                                                            >  1:843240/1‑140 (MQ=255)

                                      tCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCAGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAAcc                                                                           >  2:602428/1‑140 (MQ=255)

                                       ccGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCAGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACcg                                                                          >  1:668886/1‑140 (MQ=255)

                                         ggACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCAGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACcgcg                                                                        <  2:668886/140‑1 (MQ=255)

                                         ggACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCAGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACcgcg                                                                        <  1:789499/140‑1 (MQ=255)

                                            cGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCAGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGcca                                                                     >  1:1010488/1‑140 (MQ=255)

                                              cccGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCAGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGccacc                                                                   >  1:346416/1‑140 (MQ=255)

                                                         ttGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCAGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTg                                                        >  1:313954/1‑140 (MQ=255)

                                                                  aaaGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCAGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCaaaa                                               <  2:843240/140‑1 (MQ=255)

                                                                       gTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCAGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCa                                          <  2:313954/140‑1 (MQ=255)

                                                                       gTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCAGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCa                                          <  1:913257/140‑1 (MQ=255)

                                                                       gTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCAGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCa                                          <  1:817853/140‑1 (MQ=255)

                                                                        tCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCAGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCAt                                         >  2:329109/1‑140 (MQ=255)

                                                                          cGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCAGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTc                                       >  1:315982/1‑140 (MQ=255)

                                                                                  ccTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCAGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGAc                               <  2:346416/140‑1 (MQ=255)

                                                                                       cACCAGACGGGCCACAACAGCGGCGTGATCCATGCCAGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGTGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTg                          >  2:566484/1‑140 (MQ=255)

                                                                                       cACCAGACGGGCCACAACAGCGGCGTGATCCATGCCAGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTg                          <  1:165727/140‑1 (MQ=255)

                                                                                            gACGGGCCACAACAGCGGCGTGATCCATGCCAGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCTATCAAAACGGCATTCGCTACGACAACTGCGGCa                     <  2:1010488/140‑1 (MQ=255)

                                                                                            gACGGGCCACAACAGCGGCGTGATCCATGCCAGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCa                     >  1:43725/1‑140 (MQ=255)

                                                                                                ggCCACAACAGCGGCGTGATCCATGCCAGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGc                      <  1:80097/135‑1 (MQ=255)

                                                                                                ggCCACAACAGCGGCGTGATCCATGCCAGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGc                      >  2:80097/1‑135 (MQ=255)

                                                                                                ggCCACAACAGCGGCGTGATCCATGCCAGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGAt                 >  1:158688/1‑140 (MQ=255)

                                                                                                ggCCACAACAGCGGCGTGATCCATGCCAGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGAt                 >  1:217850/1‑140 (MQ=255)

                                                                                                 gCCACAACAGCGGCGTGATCCATGCCAGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATg                <  2:43725/140‑1 (MQ=255)

                                                                                                 gCCACAACAGCGGCGTGATCCATGCCAGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATg                <  1:329109/140‑1 (MQ=255)

                                                                                                    acaacaGCGGCGTGATCCATGCCAGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTg             <  1:602428/140‑1 (MQ=255)

                                                                                                           cggcgTGATCCATGCCAGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCa      >  2:386619/1‑140 (MQ=255)

                                                                                                           cggcgTGATCCATGCCAGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCa      >  1:819434/1‑140 (MQ=255)

                                                                                                             gcgTGATCCATGCCAGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGTGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACg    <  1:566484/140‑1 (MQ=255)

                                                                                                             gcgTGATCCATGCCAGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACg    >  1:903401/1‑140 (MQ=255)

                                                                                                               gTGATCCATGCCAGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTc  <  2:819434/140‑1 (MQ=255)

ATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTC  >  NC_000913/2790015‑2790265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A