Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	155	60.3	2039550	99.3%	2025273	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,022,559	T→C	V415A (GTA→GCA)	xdhD →	fused putative xanthine/hypoxanthine oxidase: molybdopterin‑binding subunit and Fe‑S binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,022,559	0	T	C	100.0%	180.6 / NA	51	V415A (GTA→GCA)	xdhD	fused putative xanthine/hypoxanthine oxidase: molybdopterin‑binding subunit and Fe‑S binding subunit
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (27/24); total (27/24)

CCGATGCCGATGTGATCATTGAGCGAACCTATAACTCAACGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGTACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGTGCG  >  NC_000913/3022433‑3022687

ccgatgccgatgTGATCATTGAGCGAACCTATAACTCAACGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTa                                                                                                                     <  2:395092/140‑1 (MQ=255)

ccgatgccgatgTGATCATTGAGCGAACCTATAACTCAACGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTa                                                                                                                     <  1:318823/140‑1 (MQ=255)

          tgtgATCATTGAGCGAACCTATAACTCAACGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAgg                                                                                                           >  1:866848/1‑140 (MQ=255)

            tgATCATTGAGCG‑ACCTATAACTCAACGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTcg                                                                                                        <  2:875904/140‑1 (MQ=255)

             gATCATTGAGCGAACCTATAACTCAACGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTcg                                                                                                        <  2:205751/140‑1 (MQ=255)

              aTCATTGAGCGAACCTATAACTCAACGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTcgc                                                                                                       >  2:293187/1‑140 (MQ=255)

                 aTTGAGCGAACCTATAACTCAACGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTcgcgcg                                                                                                    >  2:637887/1‑140 (MQ=255)

                   tGAGCGAACCTATAACTCAACGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGcc                                                                                                  >  2:625141/1‑140 (MQ=255)

                      gCGAACCTATAACTCAACGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCg                                                                                               >  1:151865/1‑140 (MQ=255)

                          aCCTATAACTCAACGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTggg                                                                                           >  2:370667/1‑140 (MQ=255)

                                aaCTCAACGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGaa                                                                                     <  1:293187/140‑1 (MQ=255)

                                 aCTCAACGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGaaa                                                                                    <  1:637887/140‑1 (MQ=255)

                                     aaCGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGc                                                                                >  2:963164/1‑140 (MQ=255)

                                     aaCGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGc                                                                                >  2:106567/1‑140 (MQ=255)

                                      aCGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGTCTCGTGGGCATGAAACAGCa                                                                               >  2:998024/1‑140 (MQ=255)

                                      aCGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCa                                                                               >  2:208199/1‑140 (MQ=255)

                                      aCGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCa                                                                               >  2:237053/1‑140 (MQ=255)

                                      aCGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCa                                                                               >  2:583937/1‑140 (MQ=255)

                                      aCGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCa                                                                               <  1:237053/140‑1 (MQ=255)

                                      aCGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCa                                                                               <  1:377218/140‑1 (MQ=255)

                                               cagcagTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCa                                                                      <  1:208199/140‑1 (MQ=255)

                                               cagcagTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCa                                                                      <  1:379142/140‑1 (MQ=255)

                                                                    caTATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTgg                                                 >  1:418821/1‑140 (MQ=255)

                                                                     atatCCGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTggc                                                <  2:566562/140‑1 (MQ=255)

                                                                         cTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGgcggcg                                            >  2:511487/1‑140 (MQ=255)

                                                                         cTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGgcggcg                                            >  2:558032/1‑140 (MQ=255)

                                                                             tttACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGTCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGttt                                        <  1:998024/140‑1 (MQ=255)

                                                                             tttACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTggcgg                                              >  2:232178/1‑134 (MQ=255)

                                                                             tttACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTggcgg                                              <  1:232178/134‑1 (MQ=255)

                                                                               tACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTg                                      >  1:1004827/1‑140 (MQ=255)

                                                                                 cTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGt                                    >  2:13113/1‑140 (MQ=255)

                                                                                 cTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGt                                    >  1:95867/1‑140 (MQ=255)

                                                                                  tCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGtt                                   <  2:151865/140‑1 (MQ=255)

                                                                                  tCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGtt                                   <  2:841790/140‑1 (MQ=255)

                                                                                  tCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGtt                                   <  1:112257/140‑1 (MQ=255)

                                                                                      aTGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCATCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCaa                               >  2:419838/1‑140 (MQ=255)

                                                                                      aTGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGtt                                   <  2:566500/136‑1 (MQ=255)

                                                                                      aTGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGtt                                   >  1:566500/1‑136 (MQ=255)

                                                                                              cGATCGTCTGGTTATCCACGCCTCCATCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACa                       >  1:307148/1‑140 (MQ=255)

                                                                                                aTCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACTGGACATc                     <  1:558032/140‑1 (MQ=255)

                                                                                                aTCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATc                     <  1:13113/140‑1 (MQ=255)

                                                                                                  cGTCTGGTTATCCACGCCTCCATCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCct                   <  2:307148/140‑1 (MQ=255)

                                                                                                  cGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCct                   <  1:511487/140‑1 (MQ=255)

                                                                                                    tCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCctgc                 >  2:109052/1‑140 (MQ=255)

                                                                                                    tCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCctgc                 <  1:963164/140‑1 (MQ=255)

                                                                                                    tCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCctgc                 <  1:106567/140‑1 (MQ=255)

                                                                                                      tGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCctgctg               >  1:352293/1‑140 (MQ=255)

                                                                                                               aCGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAgtg      <  1:241602/140‑1 (MQ=255)

                                                                                                                  ccTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGTgc   >  2:575616/1‑140 (MQ=255)

                                                                                                                  ccTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGTgc   >  2:150615/1‑140 (MQ=255)

                                                                                                                   cTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGTgcg  >  2:280147/1‑140 (MQ=255)

CCGATGCCGATGTGATCATTGAGCGAACCTATAACTCAACGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGTACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGTGCG  >  NC_000913/3022433‑3022687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A