Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F28 I1 R1 155 60.3 2039550 99.3% 2025273 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,177,091 T→C T64A (ACT→GCT)  yqiB ← DUF1249 domain‑containing protein YqiB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,177,0910TC98.5% 235.9 / ‑4.4 68T64A (ACT→GCT) yqiBDUF1249 domain‑containing protein YqiB
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (40/27);  total (41/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

AACTTTTTATTAGGATAATCATACCGCGCTTTGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTAGTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGGCGCAATTGTGAAAAG  >  NC_000913/3176955‑3177227
                                                                                                                                        |                                                                                                                                        
aaCTTTTTATTAGGATAATCATACCGCGCTTTGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTg                                                                                                                                       <  2:759038/140‑1 (MQ=255)
       tattaGGATAATCATACCGCGCTTTGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTAtt                                                                                                                                <  2:592689/140‑1 (MQ=255)
       tattaGGATAATCATACCGCGCTTTGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTAtt                                                                                                                                <  2:273001/140‑1 (MQ=255)
       tattaGGATAATCATACCGCGCTTTGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTAtt                                                                                                                                <  2:549757/140‑1 (MQ=255)
         ttaGGATAATCATACCGCGCTTTGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTATTGt                                                                                                                              <  1:931722/140‑1 (MQ=255)
            ggATAATCATACCGCGCTTTGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTATTGTCa                                                                                                                            >  2:285891/1‑139 (MQ=255)
            ggATAATCATACCGCGCTTTGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTATTGTCa                                                                                                                            <  1:285891/139‑1 (MQ=255)
              aTAATCATACCGCGCTTTGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTATTGTCACCa                                                                                                                         <  1:737911/140‑1 (MQ=255)
                      aCCGCGCTTTGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTATTGTCACCAGGGTAgtg                                                                                                                 >  2:139079/1‑140 (MQ=255)
                       ccGCGCTTTGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTATTGTCACCAgg                                                                                                                       <  1:871377/133‑1 (MQ=255)
                       ccGCGCTTTGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTATTGTCACCAgg                                                                                                                       >  2:871377/1‑133 (MQ=255)
                       ccGCGCTTTGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTATTGTCACCAGGGTa                                                                                                                    >  2:338338/1‑136 (MQ=255)
                       ccGCGCTTTGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTATTGTCACCAGGGTa                                                                                                                    <  1:338338/136‑1 (MQ=255)
                       ccGCGCTTTGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTATTGTCACCAGGGTAgtgt                                                                                                                >  1:468810/1‑140 (MQ=255)
                       ccGCGCTTTGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTATTGTCACCAGGGTAgtgt                                                                                                                >  2:732044/1‑140 (MQ=255)
                        cgcgCTTTGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTATTGTCAc                                                                                                                           >  1:278388/1‑128 (MQ=255)
                        cgcgCTTTGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTATTGTCAc                                                                                                                           <  2:278388/128‑1 (MQ=255)
                        cgcgCTTTGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTATTGTCACCAGGGTAGTGTa                                                                                                               >  1:970340/1‑140 (MQ=255)
                        cgcgCTTTGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTATTGTCACCAGGGTAGTGTa                                                                                                               >  1:108874/1‑140 (MQ=255)
                          cgcTTTGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTATTGTCACCa                                                                                                                         <  1:141774/128‑1 (MQ=255)
                          cgcTTTGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTATTGTCACCa                                                                                                                         >  2:141774/1‑128 (MQ=255)
                          cgcTTTGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTATTGTCACCAGGGTAGTGTATc                                                                                                             >  2:543582/1‑140 (MQ=255)
                          cgcTTTGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTATTGTCACCAGGGTAGTGTATc                                                                                                             >  1:479583/1‑140 (MQ=255)
                          cgcTTTGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTATTGTCACCAGGGTAGTGTATc                                                                                                             >  1:195157/1‑140 (MQ=255)
                           gcTTTGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTATTGTCACCAGGGTAGTGTATCg                                                                                                            >  1:300169/1‑140 (MQ=255)
                            cTTTGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTATTGTCACCa                                                                                                                         <  1:174290/126‑1 (MQ=255)
                            cTTTGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTATTGTCACCa                                                                                                                         >  2:174290/1‑126 (MQ=255)
                            cTTTGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTATTGTCACCAgg                                                                                                                       >  2:743802/1‑128 (MQ=255)
                            cTTTGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTATTGTCACCAgg                                                                                                                       <  1:743802/128‑1 (MQ=255)
                            cTTTGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTATTGTCACCAGGGTAGTGTATCgg                                                                                                           >  1:231089/1‑140 (MQ=255)
                                      aaaaaTCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTATTGTCACCAGGGTAGTGTATCGGGTCGATTCca                                                                                                 <  2:479583/140‑1 (MQ=255)
                                            ctgctgGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTATTGTCACCAGGGTAGTGTATCGGGTCGATTCCACAATCg                                                                                           <  2:600207/140‑1 (MQ=255)
                                                                 aGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTATTGTCACCAGGGTAGTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTg                                                                      <  1:543582/140‑1 (MQ=255)
                                                                  gCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTATTGTCACCAGGGTAGTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGc                                                                     <  1:139079/140‑1 (MQ=255)
                                                                      ccaTCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTATTGTCACCAGGGTAGTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCAcc                                                                 >  1:1005754/1‑140 (MQ=255)
                                                                      ccaTCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTATTGTCACCAGGGTAGTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCAcc                                                                 <  2:970340/140‑1 (MQ=255)
                                                                        aTCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTATTGTCACCAGGGTAGTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTg                                                               >  2:131933/1‑140 (MQ=255)
                                                                        aTCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTATTGTCACCAGGGTAGTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTg                                                               >  2:119308/1‑140 (MQ=255)
                                                                            cgTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTAGTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAg                                                           >  1:825555/1‑140 (MQ=255)
                                                                                         aCGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTATTGTCACCAGGGTAGTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGc                                              >  1:19376/1‑140 (MQ=255)
                                                                                         aCGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTATTGTCACCAGGGTAGTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGc                                              >  1:402788/1‑140 (MQ=255)
                                                                                          cGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTATTGTCACCAGGGTAGTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGcc                                             <  1:554490/140‑1 (MQ=255)
                                                                                          cGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTATTGTCACCAGGGTAGTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGcc                                             <  1:732044/140‑1 (MQ=255)
                                                                                           gCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTATTGTCACCAGGGTAGTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCg                                            >  2:220856/1‑140 (MQ=255)
                                                                                            cACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTATTGTCACCAGGGTAGTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCgg                                           <  2:583768/140‑1 (MQ=255)
                                                                                            cACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTATTGTCACCAGGGTAGTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCgg                                           >  2:322275/1‑140 (MQ=255)
                                                                                              ccGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTATTGTCACCAGGGTAGTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGt                                         >  2:150244/1‑140 (MQ=255)
                                                                                              ccGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTATTGTCACCAGGGTAGTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGt                                         >  1:619227/1‑140 (MQ=255)
                                                                                               cGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTATTGTCACCAGGGTAGTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTg                                        >  1:53203/1‑140 (MQ=255)
                                                                                                      gACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTATTGTCACCAGGGTAGTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCAtt                                 <  2:300169/140‑1 (MQ=255)
                                                                                                       aCGGAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTATTGTCACCAGGGTAGTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTg                                >  1:825983/1‑140 (MQ=255)
                                                                                                         ggAAGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTATTGTCACCAGGGTAGTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTgcg                              <  2:825983/140‑1 (MQ=255)
                                                                                                           aaGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTATTGTCACCAGGGTAGTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGcgcg                            <  2:108874/140‑1 (MQ=255)
                                                                                                            aGGCTCCAGTAACTGATCGCGGGCGCAGCCTGTTCTATTGTCACCAGGGTAGTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCgg                           >  1:515649/1‑140 (MQ=255)
                                                                                                                         tGATCGCGGGCGCAGCCTGTTCTATTGTCACCAGGGTAGTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGgcgc              >  2:233827/1‑140 (MQ=255)
                                                                                                                          gATCGCGGGCGCAGCCTGTTCTATTGTCACCAGGGTAGTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGgcg               >  1:7160/1‑138 (MQ=255)
                                                                                                                          gATCGCGGGCGCAGCCTGTTCTATTGTCACCAGGGTAGTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGgcg               <  2:7160/138‑1 (MQ=255)
                                                                                                                          gATCGCGGGCGCAGCCTGTTCTATTGTCACCAGGGTAGTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGGCGCa             >  2:957247/1‑140 (MQ=255)
                                                                                                                             cgcgGGCGCAGCCTGTTCTATTGTCACCAGGGTAGTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGGCGCAAtt          >  2:249874/1‑140 (MQ=255)
                                                                                                                              gcgGGCGCAGCCTGTTCTATTGTCACCAGGGTAGTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGGCGCAATtg         >  2:234942/1‑140 (MQ=255)
                                                                                                                               cgGGCGCAGCCTGTTCTATTGTCACCAGGGTAGTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGGCGCAATtgt        >  2:313422/1‑140 (MQ=255)
                                                                                                                               cgGGCGCAGCCTGTTCTATTGTCACCAGGGTAGTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGGCGCAATtgt        >  2:1004637/1‑140 (MQ=255)
                                                                                                                                 ggCGCAGCCTGTTCTATTGTCACCAGGGTAGTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGGCGCAATTGTGa      <  2:231089/140‑1 (MQ=255)
                                                                                                                                 ggCGCAGCCTGTTCTATTGTCACCAGGGTAGTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGGCGCAATTGTGa      >  2:19301/1‑140 (MQ=255)
                                                                                                                                   cgcAGCCTGTTCTATTGTCACCAGGGTAGTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGgcg               >  2:109667/1‑129 (MQ=255)
                                                                                                                                   cgcAGCCTGTTCTATTGTCACCAGGGTAGTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGgcg               <  1:109667/129‑1 (MQ=255)
                                                                                                                                   cgcAGCCTGTTCTATTGTCACCAGGGTAGTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGGCGCAATTGTGaaa    >  2:573434/1‑140 (MQ=255)
                                                                                                                                     cAGCCTGTTCTATTGTCACCAGGGTAGTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGGCGCAATTGTGAAAAg  >  2:854564/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                        
AACTTTTTATTAGGATAATCATACCGCGCTTTGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTAGTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGGCGCAATTGTGAAAAG  >  NC_000913/3176955‑3177227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A