Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	155	60.3	2039550	99.3%	2025273	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,349,410	T→C	S134G (AGC→GGC)	mtgA ←	peptidoglycan glycosyltransferase MtgA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,349,410	0	T	C	100.0%	114.1 / NA	33	S134G (AGC→GGC)	mtgA	peptidoglycan glycosyltransferase MtgA
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (15/18); total (15/18)

TCGACGCCAAACACACCGTCGCCAAATTCGGCGATATTCAGGTAAACCGTCAGGATACGCTTTTTGCTCCAGACCGTTTCTATCCCCAGCGTTAATCCGGCTTCCAGCCCTTTTCGAACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTGCGCTCGTTGTGCGCCAGGGCTTTCTCAATGGAAGCGACATCAAAGCCCCAGTGCT  >  NC_000913/3349286‑3349541

tCGACGCCAAACACACCGTCGCCAAATTCGGCGATATTCAGGTAAACCGTCAGGATACGCTTTTTGCTCCAGACCGTTTCTATCCCCAGCGTTAATCCGGCTTCCAGCCCTTTTCGAACCCAGCCACGCCCATCCCATaa                                                                                                                      >  1:54563/1‑140 (MQ=255)

tCGACGCCAAACACACAGTCGCCAAATTCGGCGATATTCAGGTAAACCGTCAGGATACGCTTTTTGCTCCAGACCGTTTCTATCCCCAGCGTTAATCCGGCTTCCAGCCCTTTTCGAACCCAGCCACGCCCATCCCATaa                                                                                                                      >  2:392262/1‑140 (MQ=255)

                cGTCGCCAAATTCGGCGATATTCAGGTAAACCGTCAGGATACGCTTTTTGCTCCAGACCGTTTCTATCCCCAGCGTTAATCCGGCTTCCAGCCCTTTTCGAACCCAGCCACGCCCATCCCATAAAAAGAGATTTTTGGct                                                                                                      <  2:675910/140‑1 (MQ=255)

                   cGCCAAATTCGGCGATATTCAGGTAAACCGTCAGGATACGCTTTTTGCTCCAGACCGTTTCTATCCCCAGCGTTAATCCGGCTTCCAGCCCTTTTCGAACCCAGCCACGCCCATCCCATAAAAAGAGATTTTTGGctgtc                                                                                                   >  1:715274/1‑140 (MQ=255)

                          ttCGGCGATATTCAGGTAAACCGTCAGGATACGCTTTTTGCTCCAGACCGTTTCTATCCCCAGCGTTAATCCGGCTTCCAGCCCTTTTCGAACCCAGCCACGCCCATCCCATAAAAAGAGATTTTTGGCTGTCTGTTgag                                                                                            >  1:905203/1‑140 (MQ=255)

                           tCGGCGATATTCAGGTAAACCGTCAGGATACGCTTTTTGCTCCAGACCGTTTCTATCCCCAGCGTTAATCCGGCTTCCAGCCCTTTTCGAACCCAGCCACGCCCATCCCATAAAAAGAGATTTTTGGCTGTCTGTTgaga                                                                                           <  2:591559/140‑1 (MQ=255)

                            cGGCGATATTCAGGTAAACCGTCAGGATACGCTTTTTGCTCCAGACCGTTTCTATCCCCAGCGTTAATCCGGCTTCCAGCCCTTTTCGAACCCAGCCACGCCCATCCCATAAAAAGAGATTTTTGGCTGTCTGTTGAGaa                                                                                          <  2:715274/140‑1 (MQ=255)

                             ggCGATATTCAGGTAAACCGTCAGGATACGCTTTTTGCTCCAGACCGTTTCTATCCCCAGCGTTAATCCGGCTTCCAGCCCTTTTCGAACCCAGCCACGCCCATCCCATAAAAAGAGATTTTTGGCTGTCTGTTGAGaaa                                                                                         <  2:309087/140‑1 (MQ=255)

                              gCGATATTCAGGTAAACCGTCAGGATACGCTTTTTGCTCCAGACCGTTTCTATCCCCAGCGTTAATCCGGCTTCCAGCCCTTTTCGAACCCAGCCACGCCCATCCCATAAAAAGAGATTTTTGGCTGTCTGTTGAGAAAt                                                                                        <  2:790459/140‑1 (MQ=255)

                                  tatTCAGGTAAACCGTCAGGATACGCTTTTTGCTCCAGACCGTTTCTATCCCCAGCGTTAATCCGGCTTCCAGCCCTTTTCGAACCCAGCCACGCCCATCCCATAAAAAGAGATTTTTGGCTGTCTGTTGAGAAATCGtt                                                                                    >  2:772607/1‑140 (MQ=255)

                                     tCAGGTAAACCGTCAGGATACGCTTTTTGCTCCAGACCGTTTCTATCCCCAGCGTTAATCCGGCTTCCAGCCCTTTTCGAACCCAGCCACGCCCATCCCATAAAAAGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGaa                                                                                 <  1:127705/140‑1 (MQ=255)

                                             aCCGTCAGGATACGCTTTTTGCTCCAGACCGTTTCTATCCCCAGCGTTAATCCGGCTTCCAGCCCTTTTCGAACCCAGCCACGCCCATCCCATAAAAAGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGAAGCACcgcg                                                                         <  2:168905/140‑1 (MQ=255)

                                                             ttttGCTCCAGACCGTTTCTATCCCCAGCGTTAATCCGGCTTCCAGCCCTTTTCGAACCCAGCCACGCCCATCCCATAAAAAGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTg                                                         <  1:168402/140‑1 (MQ=255)

                                                               ttGCTCCAGACCGTTTCTATCCCCAGCGTTAATCCGGCTTCCAGCCCTTTTCGAACCCAGCCACGCCCATCCCATAAAAAGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTgcg                                                       <  1:582931/140‑1 (MQ=255)

                                                                 gCTCCAGACCGTTTCTATCCCCAGCGTTAATCCGGCTTCCAGCCCTTTTCGAACCCAGCCACGCCCATCCCATAAAAAGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTGCGCt                                                     <  2:54563/140‑1 (MQ=255)

                                                                        aCCGTTTCTATCCCCAGCGTTAATCCGGCTTCCAGCCCTTTTCGAACCCAGCCACGCCCATCCCATAAAAAGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTGCGCTCGtt                                                 >  1:128016/1‑137 (MQ=255)

                                                                        aCCGTTTCTATCCCCAGCGTTAATCCGGCTTCCAGCCCTTTTCGAACCCAGCCACGCCCATCCCATAAAAAGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTGCGCTCGtt                                                 <  2:128016/137‑1 (MQ=255)

                                                                          cGTTTCTATCCCCAGCGTTAATCCGGCTTCCAGCCCTTTTCGAACCCAGCCACGCCCATCCCATAAAAAGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTGCGCTCGtt                                                 <  1:817478/135‑1 (MQ=255)

                                                                          cGTTTCTATCCCCAGCGTTAATCCGGCTTCCAGCCCTTTTCGAACCCAGCCACGCCCATCCCATAAAAAGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTGCGCTCGtt                                                 >  2:817478/1‑135 (MQ=255)

                                                                            tttCTATCCCCAGCGTTAATCCGGCTTCCAGCCCTTTTCGAACCCAGCCACGCCCATCCCATAAAAAGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTGCGCTCGTTGTGCGcc                                          <  1:392262/140‑1 (MQ=255)

                                                                            tttCTATCCCCAGCGTTAATCCGGCTTCCAGCCCTTTTCGAACCCAGCCACGCCCATCCCATAAAAAGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTGCGCTCGTTGTGCGcc                                          >  2:684175/1‑140 (MQ=255)

                                                                                    cccAGCGTTAATCCGGCTTCCAGCCCTTTTCGAACCCAGCCACGCCCATCCCATAAAAAGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTGCGCTCGTTGTGCGCCAGGGCttt                                  >  1:150075/1‑140 (MQ=255)

                                                                                       aGCGTTAATCCGGCTTCCAGCCCTTTTCGAACCCAGCCACGCCCATCCCATAAAAAGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTGCGCTCGTTGTGCGCCAGGGCt                                    >  2:404347/1‑135 (MQ=255)

                                                                                       aGCGTTAATCCGGCTTCCAGCCCTTTTCGAACCCAGCCACGCCCATCCCATAAAAAGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTGCGCTCGTTGTGCGCCAGGGCt                                    <  1:404347/135‑1 (MQ=255)

                                                                                       aGCGTTAATCCGGCTTCCAGCCCTTTTCGAACCCAGCCACGCCCATCCCATAAAAAGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTGCGCTCGTTGTGCGCCAGGGCTTtctc                               >  1:218420/1‑140 (MQ=255)

                                                                                       aGCGTTAATCCGGCTTCCAGCCCTTTTCGAACCCAGCCACGCCCATCCCATAAAAAGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTGCGCTCGTTGTGCGCCAGGGCTTtctc                               >  2:807528/1‑140 (MQ=255)

                                                                                             aaTCCGGCTTCCAGCCCTTTTCGAACCCAGCCACGCCCATCCCATAAAAAGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTGCGCTCGTTGTGCGCCAGGGCTTTCTCAATGGa                         <  1:807528/140‑1 (MQ=255)

                                                                                             aaTCCGGCTTCCAGCCCTTTTCGAACCCAGCCACGCCCATCCCATAAAAAGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTGCGCTCGTTGTGCGCCAGGGCTTTCTCAATGGa                         <  1:466766/140‑1 (MQ=255)

                                                                                                 cGGCTTCCAGCCCTTTTCGAACCCAGCCACGCCCATCCCATAAAAAGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTGCGCTCGTTGTGCGCCAGGGCTTTCTCAATGGAAGCg                     <  2:18079/140‑1 (MQ=255)

                                                                                                   gCTTCCAGCCCTTTTCGAACCCAGCCACGCCCATCCCATAAAAAGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTGCGCTCGTTGTGCGCCAGGGCTTTCTCAATGGAAGCGAc                   >  2:227127/1‑140 (MQ=255)

                                                                                                   gCTTCCAGCCCTTTTCGAACCCAGCCACGCCCATCCCATAAAAAGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTGCGCTCGTTGTGCGCCAGGGCTTTCTCAATGGAAGCGAc                   >  1:264925/1‑140 (MQ=255)

                                                                                                              ttttCGAACCCAGCCACGCCCATCCCATAAAAAGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTGCGCTCGTTGTGCGCCAGGGCTTTCTCAATGGAAGCGACATCAAAGcccc        >  1:251064/1‑140 (MQ=255)

                                                                                                                    aaCCCAGCCACGCCCATCCCATAAAAAGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTGCGCTCGTTGTGCGCCAGGGCTTTCTCAATGGAAGCGACATCAAAGCCCCAGTGCt  <  2:251064/140‑1 (MQ=255)

TCGACGCCAAACACACCGTCGCCAAATTCGGCGATATTCAGGTAAACCGTCAGGATACGCTTTTTGCTCCAGACCGTTTCTATCCCCAGCGTTAATCCGGCTTCCAGCCCTTTTCGAACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTGCGCTCGTTGTGCGCCAGGGCTTTCTCAATGGAAGCGACATCAAAGCCCCAGTGCT  >  NC_000913/3349286‑3349541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A