Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F28 I1 R1 155 60.3 2039550 99.3% 2025273 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,454,068 (T)6→7 coding (1331/1470 nt) gspA ← Type II secretion system protein GspA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,454,0621.T98.4% 227.3 / ‑4.0 64coding (1337/1470 nt)gspAType II secretion system protein GspA
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base T (36/27);  total (37/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACATGTCATTTCCCCTTCACTGTTGTTTCCGGGGAAATATTTGCTTCATCCCGATATAAGTAGGCACTCTCCCCTGCCACCTGCCAGAGATGTACTAACGTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGC‑TTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTGTCGAAATATGCAATGCGCGGTTTAACATCGTTTCCAGCCAGAGAATCTCTTCCTCGCTGCTATCACGCGTAATG  >  NC_000913/3453924‑3454196
                                                                                                                                           |                                                                                                                                       
aCATGTCATTTCCCCTTCACTGTTGTTTCCGGGGAAATATTTGCTTCATCCCGATATAAGTAGGCACTCTCCCCTGCCACCTGCCAGAGATGTACTAACGTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGC‑t                                                                                                                                        <  2:526254/140‑1 (MQ=255)
              cTTCACTGTTGTTTCCGGGGAAATATTTGCTTCATCCCGATATAAGTAGGCACTCTCCCCTGCCACCTGCCAGAGATGTACTAACGTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGc                                                                                                                          >  2:148825/1‑140 (MQ=255)
                 cACTGTTGTTTCCGGGGAAATATTTGCTTCATCCCGATATAAGTAGGCACTCTCCCCTGCCACCTGCCAGAGATGTACTAACGTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCttt                                                                                                                       >  1:62316/1‑140 (MQ=255)
                  aCTGTTGTTTCCGGGGAAATATTTGCTTCATCCCGATATAAGTAGGCACTCTCCCCTGCCACCTGCCAGAGATGTACTAACGTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTa                                                                                                                      <  1:214409/140‑1 (MQ=255)
                    tgttgtTTCCGGGGAAATATTTGCTTCATCCCGATATAAGTAGGCACTCTCCCCTGCCACCTGCCAGAGATGTACTAACGTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTAtt                                                                                                                    >  1:460622/1‑140 (MQ=255)
                          ttCCGGGGAAATATTTGCTTCATCCCGATATAAGTAGGCACTCTCCCCTGCCACCTGCCAGAGATGTACTAACGTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCt                                                                                                              <  2:890547/140‑1 (MQ=255)
                                ggAAATATTTGCTTCATCCCGATATAAGTAGGCACTCTCCCCTGCCACCTGCCAGAGATGTACTAACGTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACcaac                                                                                                        >  1:301557/1‑140 (MQ=255)
                                   aaTATTTGCTTCATCCCGATATAAGTAGGCACTCTCCCCTGCCACCTGCCAGAGATGTACTAACGTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAg                                                                                                     <  1:990410/140‑1 (MQ=255)
                                        ttGCTTCATCCCGATATAAGTAGGCACTCTCCCCTGCCACCTGCCAGAGATGTACTAACGTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACg                                                                                                >  2:170129/1‑140 (MQ=255)
                                           cTTCATCCCGATATAAGTAGGCACTCTCCCCTGCCACCTGCCAGAGATGTACTAACGTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCa                                                                                             <  2:460622/140‑1 (MQ=255)
                                              cATCCCGATATAAGTAGGCACTCTCCCCTGCCACCTGCCAGAGATGTACTAACGTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTc                                                                                          >  2:542818/1‑140 (MQ=255)
                                               aTCCCGATATAAGTAGGCACTCTCCCCTGCCACCTGCCAGAGATGTACTAACGTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTcc                                                                                         >  1:825164/1‑140 (MQ=255)
                                               aTCCCGATATAAGTAGGCACTCTCCCCTACCACCTGCCAGAGATGTACTAACGTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTcc                                                                                         >  1:355340/1‑140 (MQ=255)
                                                  ccGATATAAGTAGGCACTCTCCCCTGCCACCTGCCAGAGATGTACTAACGTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTccgcc                                                                                      <  1:728279/140‑1 (MQ=255)
                                                          aGTAGGCACTCTCCCCTGCCACCTGCCAGAGATGTACTAACGTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTc                                                                              >  1:614040/1‑140 (MQ=255)
                                                            tAGGCACTCTCCCCTGCCACCTGCCAGAGATGTACTAACGTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTg                                                                            >  1:304240/1‑140 (MQ=255)
                                                            tAGGCACTCTCCCCTGCCACCTGCCAGAGATGTACTAACGTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTg                                                                            <  1:542818/140‑1 (MQ=255)
                                                              ggCACTCTCCCCTGCCACCTGCCAGAGATGTACTAACGTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTGTc                                                                          >  1:338144/1‑140 (MQ=255)
                                                              ggCACTCTCCCCTGCCACCTGCCAGAGATGTACTAACGTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTGTc                                                                          <  2:338144/140‑1 (MQ=255)
                                                               gCACTCTCCCCTGCCACCTGCCAGAGATGTACTAACGTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTGTCg                                                                         >  1:85629/1‑140 (MQ=255)
                                                               gCACTCTCCCCTGCCACCTGCCAGAGATGTACTAACGTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTGTCg                                                                         >  1:759856/1‑140 (MQ=255)
                                                                cACTCTCCCCTGCCACCTGCCAGAGATGTACTAACGTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTGTCGa                                                                        <  2:884348/140‑1 (MQ=255)
                                                                cACTCTCCCCTGCCACCTGCCAGAGATGTACTAACGTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTGTCGa                                                                        >  1:111855/1‑140 (MQ=255)
                                                                 aCTCTCCCCTGCCACCTGCCAGAGATGTACTAACGTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTGTCGaa                                                                       <  2:614040/140‑1 (MQ=255)
                                                                     tcCCCTGCCACCTGCCAGAGATGTACTAACGTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTGTCGAAatat                                                                   >  2:979036/1‑140 (MQ=255)
                                                                        cctgccacctgccaGAGATGTACTAACGTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTGTCGAAATATGCa                                                                <  2:111855/140‑1 (MQ=255)
                                                                             cacctgccaGAGATGTACTAACGTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTGTCGAAATATGCAATgcg                                                           <  1:727183/140‑1 (MQ=255)
                                                                             cacctgccaGAGATGTACTAACGTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTGTCGAAATATGCAATgcg                                                           >  2:359537/1‑140 (MQ=255)
                                                                             cacctgccaGAGATGTACTAACGTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTGTCGAAATATGCAATgcg                                                           >  2:247624/1‑140 (MQ=255)
                                                                             cacctgccaGAGATGTACTAACGTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTGTCGAAATATGCAATgcg                                                           <  2:825164/140‑1 (MQ=255)
                                                                               cctgccaGAGATGTACTAACGTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTTTCGAAATATGCAATGcgcg                                                         >  1:698008/1‑140 (MQ=255)
                                                                                 tgccaGAGATGTACTAACGTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTGTCGAAATATGCAATGCGCGGt                                                       >  2:83581/1‑140 (MQ=255)
                                                                                        gaTGTACTAACGTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTGTCGAAATATGCAATGCGCGGTTTAACAt                                                <  1:274895/140‑1 (MQ=255)
                                                                                           gTACTAACGTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTGTCGAAATATGCAATGCGCGGTTTAACATCGt                                             >  2:383622/1‑140 (MQ=255)
                                                                                            tACTAACGTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTGTCGAAATATGCAATGCGCGGTTTAACATCGtt                                            >  2:244766/1‑140 (MQ=255)
                                                                                             aCTAACGTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTGTCGAAATATGCAATGCGCGGTTTAACATCGttt                                           <  1:148825/140‑1 (MQ=255)
                                                                                                 aCGTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTGTCGAAATATGCAATGCGCGGTTTAACATCGttt                                           >  2:601987/1‑136 (MQ=255)
                                                                                                 aCGTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTGTCGAAATATGCAATGCGCGGTTTAACATCGttt                                           <  1:601987/136‑1 (MQ=255)
                                                                                                  cGTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTGTCGAAATATGCAATGCGCGGTTTAACATCGTTTccagc                                      >  2:82379/1‑140 (MQ=255)
                                                                                                  cGTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTGTCGAAATATGCAATGCGCGGTTTAACATCGTTTccagc                                      <  1:979036/140‑1 (MQ=255)
                                                                                                  cGTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTGTCGAAATATGCAATGCGCGGTTTAACATCGTTTccagc                                      <  2:301557/140‑1 (MQ=255)
                                                                                                   gTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTGTCGAAATATGCAATGCGCGGTTTAACAt                                                >  2:18526/1‑129 (MQ=255)
                                                                                                   gTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTGTCGAAATATGCAATGCGCGGTTTAACAt                                                <  1:18526/129‑1 (MQ=255)
                                                                                                   gTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTGTCGAAATATGCAATGCGCGGTTTAACATCGTTTccagcc                                     >  2:630705/1‑140 (MQ=255)
                                                                                                   gTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTGTCGAAATATGCAATGCGCGGTTTAACATCGTTTccagcc                                     >  2:603203/1‑140 (MQ=255)
                                                                                                   gTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTGTCGAAATATGCAATGCGCGGTTTAACATCGTTTccagcc                                     >  1:291364/1‑140 (MQ=255)
                                                                                                   gTTGAAAAGCCAACAACACCGTCAGTTTTCAAGGGGGGGC‑TTTTTGTGAAAGTACTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTGTCGAAATATGCAATGCGCGGTTTAACATCGTTTccagcc                                     >  2:1012310/1‑140 (MQ=255)
                                                                                                    ttGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTGTCGAAATATGCAATGCGCGGTTTAACATCGTTTccagcca                                    <  1:82379/140‑1 (MQ=255)
                                                                                                     tGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTGTCGAAATATGCAATGCGCGGTTTAACATCGTTTccagccag                                   <  1:102569/140‑1 (MQ=255)
                                                                                                      gAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTGTCGAAATATGCAATGCGCGGTTTAACATCGTTTCCAGCCaga                                  <  2:304240/140‑1 (MQ=255)
                                                                                                            ccAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTGTCGAAATATGCAATGCGCGGTTTAACATCGTTTCCAGCCAGAGAAtct                            >  1:400884/1‑140 (MQ=255)
                                                                                                            ccAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTGTCGAAATATGCAATGCGCGGTTTAACATCGTTTCCAGCCAGAGAAtct                            >  1:105607/1‑140 (MQ=255)
                                                                                                                    acCGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTGTCGAAATATGCAATGCGCGGTTTAACATCGTTTCCAGCCAGAGAATCTCTTCCTCg                    <  2:62316/140‑1 (MQ=255)
                                                                                                                     ccGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTGTCGAAATATGCAATGCGCGGTTTAACATCGTTTCCAGCCAGAGAATCTCTTCCTCgc                   >  2:718249/1‑140 (MQ=255)
                                                                                                                      cGTCAGTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTGTCGAAATATGCAATGCGCGGTTTAACATCGTTTCCAGCCAGAGAATCTCTTCCTCgct                  >  2:867169/1‑140 (MQ=255)
                                                                                                                           gTTTTCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTGTCGAAATATGCAATGCGCGGTTTAACATCGTTTCCAGCCAGAGAATCTCTTCCTCGCTGCTAt             >  1:371326/1‑140 (MQ=255)
                                                                                                                              ttCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTGTCGAAATATGCAATGCGCGGTTTAACATCGTTTCCAGCCAGAGAATCTCTTCCTCGCTGCTATCAc          >  1:926583/1‑140 (MQ=255)
                                                                                                                               tCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTGTCGAAATATGCAATGCGCGGTTTAACATCGTTTCCAGCCAGAGAATCTCTTCCTCGCTGCTATCAcg         <  2:105607/140‑1 (MQ=255)
                                                                                                                               tCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTGTCGAAATATGCAATGCGCGGTTTAACATCGTTTCCAGCCAGAGAATCTCTTCCTCGCTGCTATCAcg         <  2:926583/140‑1 (MQ=255)
                                                                                                                               tCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTGTCGAAATATGCAATGCGCGGTTTAACATCGTTTCCAGCCAGAGAATCTCTTCCTCGCTGCTATCAcg         >  2:903247/1‑140 (MQ=255)
                                                                                                                               tCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTGTCGAAATATGCAATGCGCGGTTTAACATCGTTTCCAGCCAGAGAATCTCTTCCTCGCTGCTATCAcg         <  1:903247/140‑1 (MQ=255)
                                                                                                                               tCAAGTGGTGGCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTGTCGAAATATGCAATGCGCGGTTTAACATCGTTTCCAGCCAGAGAATCTCTTCCTCGCTGCTATCAcg         <  2:355340/140‑1 (MQ=255)
                                                                                                                                      gtggCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTGTCGAAATATGCAATGCGCGGTTTAACATCGTTTCCAGCCAGAGAATCTCTTCCTCGCTGCTATCACGCGTAATg  >  1:543710/1‑140 (MQ=255)
                                                                                                                                      gtggCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTGTCGAAATATGCAATGCGCGGTTTAACATCGTTTCCAGCCAGAGAATCTCTTCCTCGCTGCTATCACGCGTAATg  >  1:243479/1‑140 (MQ=255)
                                                                                                                                      gtggCTTTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTGTCGAAATATGCAATGCGCGGTTTAACATCGTTTCCAGCCAGAGAATCTCTTCCTCGCTGCTATCACGCGTAATg  <  1:170129/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                       
ACATGTCATTTCCCCTTCACTGTTGTTTCCGGGGAAATATTTGCTTCATCCCGATATAAGTAGGCACTCTCCCCTGCCACCTGCCAGAGATGTACTAACGTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGC‑TTTTT‑TGAAATTGCTTTATTTTTTCTACCAACAAGGGACGCCACTCCGCCGAAGGTTCTGTCGAAATATGCAATGCGCGGTTTAACATCGTTTCCAGCCAGAGAATCTCTTCCTCGCTGCTATCACGCGTAATG  >  NC_000913/3453924‑3454196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A