Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	155	60.3	2039550	99.3%	2025273	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,503,667	A→G	A167A (GCA→GCG)	frlD →	fructoselysine 6‑kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,503,667	0	A	G	100.0%	219.0 / NA	62	A167A (GCA→GCG)	frlD	fructoselysine 6‑kinase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (30/32); total (30/32)

CTGGGGACATGCGGAAGACGCATTCCCACAGCTGCACGCTGCGGGCAAACTTACCGCTTTCGACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCACCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTGGCGCAGGAACAGTGATTGTCACGCTGGGTGAAAACGGCAGCATTGCCTGGGATGGCGCGCAGTTCTGGCGTCAGGCTCCTGAA  >  NC_000913/3503532‑3503802

cTGGGGACATGCGGAAGACGCATTCCCACAGCTGCACGCTGCGGGCAAACTTACCGCTTTCGACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTccgcgccgc                                                                                                                                     <  1:893952/140‑1 (MQ=255)

    ggACATGCGGAAGACGCATTCCCACAGCTGCACGCTGCGGGCAAACTTACCGCTTTCGACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCaaga                                                                                                                                 >  1:861687/1‑140 (MQ=255)

    ggACATGCGGAAGACGCATTCCCACAGCTGCACGCTGCGGGCAAACTTACCGCTTTCGACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCaaga                                                                                                                                 <  1:367579/140‑1 (MQ=255)

         tGCGGAAGACGCATTCCCACAGCTGCACACTGCGGGCAAACTTACCGCTTTCGACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTccgcgccg                                                                                                                                      <  1:77388/130‑1 (MQ=255)

         tGCGGAAGACGCATTCCCACAGCTGCACACTGCGGGCAAACTTACCGCTTTCGACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTccgcgccg                                                                                                                                      >  2:77388/1‑130 (MQ=255)

          gCGGAAGACGCATTCCCACAGCTGCACGCTGCGGGCAAACTTACCGCTTTCGACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGa                                                                                                                           <  1:991739/140‑1 (MQ=255)

           cGGAAGACGCATTCCCACAGCTGCACGCTGCGGGCAAACTTACCGCTTTCGACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGaa                                                                                                                          >  2:253073/1‑140 (MQ=255)

               aGACGCATTCCCACAGCTGCACGCTGCGGGCAAACTTACCGCTTTCGACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGc                                                                                                                      <  1:836490/140‑1 (MQ=255)

               aGACGCATTCCCACAGCTGCACGCTGCGGGCAAACTTACCGCTTTCGACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGc                                                                                                                      <  1:405255/140‑1 (MQ=255)

                gACGCATTCCCACAGCTGCACGCTGCGGGCAAACTTACCGCTTTCGACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCt                                                                                                                     <  2:636746/140‑1 (MQ=255)

                    cATTCCCACAGCTGCACGCTGCGGGCAAACTTACCGCTTTCGACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGt                                                                                                                 <  1:725481/140‑1 (MQ=255)

                            caGCTGCACGCTGCGGGCAAACTTACCGCTTTCGACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGAt                                                                                                         <  1:164908/140‑1 (MQ=255)

                            caGCTGCACGCTGCGGGCAAACTTACCGCTTTCGACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGAt                                                                                                         >  1:495449/1‑140 (MQ=255)

                             aGCTGCACGCTGCGGGCAAACTTACCGCTTTCGACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATg                                                                                                        <  2:612200/140‑1 (MQ=255)

                               cTGCACGCTGCGGGCAAACTTACCGCTTTCGACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGaa                                                                                                      <  2:124770/140‑1 (MQ=255)

                                 gCACGCTGCGGGCAAACTTACCGCTTTCGACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAg                                                                                                    >  2:988173/1‑140 (MQ=255)

                                  cACGCTGCGGGCAAACTTACCGCTTTCGACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGc                                                                                                   >  1:414189/1‑140 (MQ=255)

                                   aCGCTGCGGGCAAACTTACCGCTTTCGACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCg                                                                                                  >  2:495279/1‑140 (MQ=255)

                                       tGCGGGCAAACTTACCGCTTTCGACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGAttg                                                                                              >  2:996119/1‑140 (MQ=255)

                                       tGCGGGCAAACTTACCGCTTACGACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGAttg                                                                                              >  1:920273/1‑140 (MQ=255)

                                               aaCTTACCGCTTTCGACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGt                                                                                      <  1:420076/140‑1 (MQ=255)

                                                 cTTACCGCTTTCGACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTgg                                                                                    >  2:259225/1‑140 (MQ=255)

                                                 cTTACCGCTTTCGACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTgg                                                                                    >  1:234037/1‑140 (MQ=255)

                                                   tACCGCTTTCGACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGt                                                                                      <  2:410213/136‑1 (MQ=255)

                                                   tACCGCTTTCGACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGt                                                                                      >  1:410213/1‑136 (MQ=255)

                                                    aCCGCTTTCGACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTGgcgc                                                                                 <  1:853418/140‑1 (MQ=255)

                                                        cTTTCGACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTGGCGCAGGa                                                                             <  2:414189/140‑1 (MQ=255)

                                                         tttCGACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTGGCGCAGGaa                                                                            <  1:970364/140‑1 (MQ=255)

                                                             gACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTGGCGCAGGAACAGt                                                                        <  2:861687/140‑1 (MQ=255)

                                                                   tcCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTGGCGCAGGAACAGTGATTGt                                                                  >  2:443543/1‑140 (MQ=255)

                                                                       aCAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTGGCGCAGGAACAGTGATTGTCACg                                                              <  1:266312/140‑1 (MQ=255)

                                                                        cAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAATATGAAAGCGATTGTTGCCCGTGGCGCAGGAACAGTGATTGTCACGc                                                             <  1:855082/140‑1 (MQ=255)

                                                                             gggACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTGGCGCAGGAACAGTGATTGTCACGCTgggg                                                        >  2:783619/1‑139 (MQ=255)

                                                                              ggACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTGGCGCAGGAACAGTGATTGTCACGCTGGGTg                                                       >  1:611601/1‑140 (MQ=255)

                                                                                aCAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTGGCGCAGGAACAGTGATTGTCACGCTGGGTGaa                                                     <  2:725282/140‑1 (MQ=255)

                                                                                   gCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTGGCGCAGGAACAGTGATTGTCACGCTGGGTGAAAAc                                                  >  2:413311/1‑140 (MQ=255)

                                                                                   gCCCGCTCTGACAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTGGCGCAGGAACAGTGATTGTCACGCTGGGTGAAAAc                                                  >  2:426482/1‑140 (MQ=255)

                                                                                    cccGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTGGCGCAGGAACAGTGATTGTCACGCTGGGTGAAAACg                                                 >  1:182529/1‑140 (MQ=255)

                                                                                    cccGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTGGCGCAGGAACAGTGATTGTCACGCTGGGTGAAAACg                                                 >  2:387061/1‑140 (MQ=255)

                                                                                    cccGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTGGCGCAGGAACAGTGATTGTCACGCTGGGTGAAAACg                                                 >  1:176877/1‑140 (MQ=255)

                                                                                    cccGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTGGCGCAGGAACAGTGATTGTCACGCTGGGTGAAAACg                                                 >  1:562307/1‑140 (MQ=255)

                                                                                     ccGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTGGCGCAGGAACAGTGATTGTCACGCTGGGTGAAAACgg                                                <  1:413311/140‑1 (MQ=255)

                                                                                     ccGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTGGCGCAGGAACAGTGATTGTCACGCTGGGTGAAAACgg                                                >  1:91746/1‑140 (MQ=255)

                                                                                        ctctGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTGGCGCAGGAACAGTGATTGTCACGCTGGGTGAAAACGgcag                                             >  2:309173/1‑140 (MQ=255)

                                                                                         tctGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTGGCGCAGGAACAGTGATTGTCACGCTGGGTGAAAACGgcagc                                            <  2:316899/140‑1 (MQ=255)

                                                                                          ctGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTGGCGCAGGAACAGTGATTGTCACGCTGGGTGAAAACGgcagca                                           <  1:996119/140‑1 (MQ=255)

                                                                                          ctGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTGGCGCAGGAACAGTGATTGTCACGCTGGGTGAAAACGgcagca                                           <  2:562307/140‑1 (MQ=255)

                                                                                          ctGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTGGCGCAGGAACAGTGATTGTCACGCTGGGTGAAAACGgcagca                                           <  1:259225/140‑1 (MQ=255)

                                                                                          ctGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTGGCGCAGGAACAGTGATTGTCACGCTGGGTGAAAACGgcagca                                           <  1:988173/140‑1 (MQ=255)

                                                                                          ctGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTGGCGCAGGAACAGTGATTGTCACGCTGGGTGAAAACGgcagca                                           <  1:387061/140‑1 (MQ=255)

                                                                                          ctGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTGGCGCAGGAACAGTGATTGTCACGCTGGGTGAAAACGgcagca                                           <  2:1018677/140‑1 (MQ=255)

                                                                                          ctGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTGGCGCAGGAACAGTGATTGTCACGCTGGGTGAAAACGgcagca                                           <  1:64569/140‑1 (MQ=255)

                                                                                          ctGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCACGTGGCGCAGGAACAGTGATTGTCACGCTGGGTGAAAACGgcagca                                           <  2:495449/140‑1 (MQ=255)

                                                                                            ggCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTGGCGCAGGAACAGTGATTGTCACGCTGGGTGAAAACGGCAGCAtt                                         <  1:309173/140‑1 (MQ=255)

                                                                                             gCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTGGCGCAGGAACAGTGATTGTCACGCTGGGTGAAAACGgcag                                             <  1:649356/135‑1 (MQ=255)

                                                                                             gCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTGACGCAGGAACAGTGATTGTCACGCTGGGTGAAAACGgcag                                             >  2:649356/1‑135 (MQ=255)

                                                                                              cAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTGGCGCAGGAACAGTGATTGTCACGCTGGGTGAAAACGGCAGCATTGc                                       >  1:622680/1‑140 (MQ=255)

                                                                                                     tGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTGGCGCAGGAACAGTGATTGTCACGCTGGGTGAAAACGGCAGCATTGCCTGGGAt                                >  1:228932/1‑140 (MQ=255)

                                                                                                       gTGCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTGGCGCAGGAACAGTGATTGTCACGCTGGGTGGAAACGGCAGCATTGCCTGGGATgg                              >  2:263821/1‑140 (MQ=255)

                                                                                                         gCCGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTGGCGCAGGAACAGTGATTGTCACGCTGGGTGAAAACGGCAGCATTGCCTGGGATGgcg                            >  1:256139/1‑140 (MQ=255)

                                                                                                           cGCATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTGGCGCAGGAACAGTGATTGTCACGCTGGGTGAAAACGGCAGCATTGCCTGGGATGgcgcg                          <  2:329636/140‑1 (MQ=255)

                                                                                                             cATCTCGATTTTGCCTTTGCCTCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTGGCGCAGGAACAGTGATTGTCACGCTGGGTGAAAACGGCAGCATTGCCTGGGATGGCGCGCa                        >  1:415394/1‑140 (MQ=255)

                                                                                                                         gcctttgcctCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTGGCGCAGGAACAGTGATTGTCACGCTGGGTGAAAACGGCAGCATTGCCTGGGATGGCGCGCAGTTCTGGCGTCa            <  2:256139/140‑1 (MQ=255)

                                                                                                                          cctttgcctCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTGGCGCAGGAACAGTGATTGTCACGCTGGGTGAAAACGGCGGCATTGCCTGGGATGGCGCGCAGTTCTGGCGTCAg           >  1:648663/1‑140 (MQ=255)

                                                                                                                               gcctCCGCGCCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTGGCGCAGGAACAGTGATTGTCACGCTGGGTGAAAACGGCAGCATTGCCTGGGATGGCGCGCAGTTCTGGCGTCAGGCTcc      >  2:125479/1‑140 (MQ=255)

                                                                                                                                   ccgcgccgcaAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTGGCGCAGGAACAGTGATTGTCACGCTGGGTGAAAACGGCAGCATTGCCTGGGATGGCGCGCAGTTCTGGCGTCAGGCTCCTGaa  <  1:426482/140‑1 (MQ=255)

CTGGGGACATGCGGAAGACGCATTCCCACAGCTGCACGCTGCGGGCAAACTTACCGCTTTCGACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCACCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTGGCGCAGGAACAGTGATTGTCACGCTGGGTGAAAACGGCAGCATTGCCTGGGATGGCGCGCAGTTCTGGCGTCAGGCTCCTGAA  >  NC_000913/3503532‑3503802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A