Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	155	60.3	2039550	99.3%	2025273	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,588,939	A→G	V81A (GTG→GCG)	ugpC ←	sn‑glycerol 3‑phosphate ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,588,939	0	A	G	100.0%	141.6 / NA	40	V81A (GTG→GCG)	ugpC	sn‑glycerol 3‑phosphate ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (17/23); total (17/23)

ACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCCGCTCCAGCCCGGCAACCATGCGCAGCAGCGTCGATTTCCCGCAGCCAGACGGCCCGA  >  NC_000913/3588803‑3589074

aCCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACGCCa                                                                                                                                      >  1:84238/1‑140 (MQ=255)

  cGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACGCCATc                                                                                                                                    >  1:81822/1‑140 (MQ=255)

   gTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACGCCATCg                                                                                                                                   >  2:133906/1‑140 (MQ=255)

            cAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACGCCATCGCAATCCcgc                                                                                                                          <  1:661877/140‑1 (MQ=255)

                  gcgcgcCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACGCCATCGCAATCCCGCGATCtt                                                                                                                    <  1:475870/140‑1 (MQ=255)

                    gcgcCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTGAGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACGCCATCGCAATCCCGCGATCtttt                                                                                                                  <  2:909266/140‑1 (MQ=255)

                     cgccgcTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACGCCATCGCAATCCCGCGATCTTTTg                                                                                                                 >  1:314747/1‑140 (MQ=255)

                      gccgcTTCTTTAACGCGCTCGGCAATTTGCAGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACGCCAACGCAATCCCGCGATCTTTTgg                                                                                                                <  1:625155/140‑1 (MQ=255)

                        cgcTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACGCCATCGCAATCCCGCGATCTTTTGGCt                                                                                                              <  2:471787/140‑1 (MQ=255)

                                  aCGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACGCCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGt                                                                                                    >  2:724277/1‑140 (MQ=255)

                                       cTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACGCCATCGCAATCCCGCGATCTTTTGGCTc                                                                                                             >  1:324172/1‑126 (MQ=255)

                                       cTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACGCCATCGCAATCCCGCGATCTTTTGGCTc                                                                                                             <  2:324172/126‑1 (MQ=255)

                                       cTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACGCCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCAcgc                                                                                               <  1:133906/140‑1 (MQ=255)

                                        tCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACGCCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCAcgcg                                                                                              <  1:507175/140‑1 (MQ=255)

                                        tCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACGCCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCAcgcg                                                                                              <  2:535062/140‑1 (MQ=255)

                                        tCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACGCCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCAcgcg                                                                                              <  1:566755/140‑1 (MQ=255)

                                         cGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACGCCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACgcgc                                                                                             <  2:815068/140‑1 (MQ=255)

                                                              tGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACGCCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCg                                                                        <  2:474570/140‑1 (MQ=255)

                                                              tGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACGCCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCg                                                                        >  1:196203/1‑140 (MQ=255)

                                                                ccGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACGCCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGcc                                                                      <  2:158035/140‑1 (MQ=255)

                                                                         ttCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACGCCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCAc                                                             <  2:73190/140‑1 (MQ=255)

                                                                          tCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACGCCATCGCAATCCCGCGATCTTTTGGCTCCATTTCGGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCAcc                                                            <  2:314747/140‑1 (MQ=255)

                                                                                  cACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACGCCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCCGCTCCAg                                                    <  1:724277/140‑1 (MQ=255)

                                                                                          gTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACGCCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCCGCTCCAGCCCGGCaa                                            <  1:178683/140‑1 (MQ=255)

                                                                                                ttcGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACGCCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCCGCTCCAGCCCGGCAACCATgc                                      >  1:329862/1‑140 (MQ=255)

                                                                                                   gACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACGCCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCCGCTCCAGCCCGGCAACCATGCgca                                   >  1:112116/1‑140 (MQ=255)

                                                                                                     cacTCATATGCGGATAAAGCGCGTAGTTCTGGAACGCCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCCGCTCCAGCCCGGCAACCATGCgcagc                                 >  1:832082/1‑140 (MQ=255)

                                                                                                       cTCATATGCGGATAAAGCGCGTAGTTCTGGAACGCCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCCGCTCCAGCCCGGCAACCATGCGcagcag                               <  2:84238/140‑1 (MQ=255)

                                                                                                        tCATATGCGGATAAAGCGCGTAGTTCTGGAACGCCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCCGCTCCAGCCCgg                                               <  2:419204/123‑1 (MQ=255)

                                                                                                        tCATATGCGGATAAAGCGCGTAGTTCTGGAACGCCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCCGCTCCAGCCCgg                                               >  1:419204/1‑123 (MQ=255)

                                                                                                          atatGCGGATAAAGCGCGTAGTTCTGGAACGCCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCCGCTCCAGCCCGGCAACCATGCGCAGCAGCGt                            >  2:836450/1‑140 (MQ=255)

                                                                                                            atGCGGATAAAGCGCGTAGTTCTGGAACGCCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCCGCTCCAGCCCGGCAACCATGCGCAGCAGCGTCg                          >  1:476693/1‑140 (MQ=255)

                                                                                                                  aTAAAGCGCGTAGTTCTGGAACGCCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCCGCTCCAGCCCGGCAACCATGCGCAGCAGCGTCGATTTcc                    >  2:519068/1‑140 (MQ=255)

                                                                                                                    aaaGCGCGTAGTTCTGGAACGCCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCCGCTCCAGCCCGGCAACCATGCGCAGCAGCGTCGATTTCCCg                  >  1:928185/1‑140 (MQ=255)

                                                                                                                    aaaGCGCGTAGTTCTGGAACGCCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCCGCTCCAGCCCGGCAACCATGCGCAGCAGCGTCGATTTCCCg                  <  1:519068/140‑1 (MQ=255)

                                                                                                                      aGCGCGTAGTTCTGGAACGCCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCCGCTCCAGCCCGGCAACCATGCGCAGCAGCGTCGATTTCCCGCa                >  1:128032/1‑140 (MQ=255)

                                                                                                                           gTAGTTCTGGAACGCCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCCGCTCCAGCCCGGCAACCATGCGCAGCAGCGTCGATTTCCCGCAGCCAg           <  2:832082/140‑1 (MQ=255)

                                                                                                                               ttCTGGAACGCCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCCGCTCCAGCCCGGCAACCATGCGCAGCAGCGTCGATTTCCCGCAGCCAGACgg       <  2:196203/140‑1 (MQ=255)

                                                                                                                                tCTGGAACGCCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCCGCTCCAGCCCGGCAACCATGCGCAGCAGCGTCGATTTCCCGCAGCCAGACGGc      <  2:112116/140‑1 (MQ=255)

                                                                                                                                    gAACGCCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCCGCTCCAGCCCGGCAACCATGCGCAGCAGCGTCGATTTCCCGCAGCCAGACGGCCCGa  >  2:794953/1‑140 (MQ=255)

ACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCCGCTCCAGCCCGGCAACCATGCGCAGCAGCGTCGATTTCCCGCAGCCAGACGGCCCGA  >  NC_000913/3588803‑3589074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A