Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F28 I1 R1 155 60.3 2039550 99.3% 2025273 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,602,893 T→C T451A (ACG→GCG)  ftsY ← signal recognition particle receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,602,8930TC100.0% 242.0 / NA 68T451A (ACG→GCG) ftsYsignal recognition particle receptor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/35);  total (33/35)

CTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTC  >  NC_000913/3602758‑3603022
                                                                                                                                       |                                                                                                                                 
ctcGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTcgccgcgccg                                                                                                                               >  2:553078/1‑140 (MQ=255)
ctcGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTcgccgcgccg                                                                                                                               >  2:897971/1‑140 (MQ=255)
ctcGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAACCACCGAGAAAATTACCCCGCCTTTcgccgcgccg                                                                                                                               >  1:859399/1‑140 (MQ=255)
 tcGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCgccgcgccgt                                                                                                                              <  1:373046/140‑1 (MQ=255)
  cGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTc                                                                                                                             >  2:643379/1‑140 (MQ=255)
               ccTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGc                                                                                                                >  1:305539/1‑140 (MQ=255)
                   tataAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGa                                                                                                            >  2:174686/1‑140 (MQ=255)
                    ataAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGAt                                                                                                           <  1:174686/140‑1 (MQ=255)
                     taAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATg                                                                                                          >  2:408133/1‑140 (MQ=255)
                      aaaGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGc                                                                                                         <  1:897971/140‑1 (MQ=255)
                             tcCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGtt                                                                                                                   >  1:474874/1‑123 (MQ=255)
                             tcCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGtt                                                                                                                   <  2:474874/123‑1 (MQ=255)
                             tcCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTaa                                                                                                  >  1:348765/1‑140 (MQ=255)
                             tcCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTaa                                                                                                  >  2:151988/1‑140 (MQ=255)
                             tcCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTaa                                                                                                  >  1:515779/1‑140 (MQ=255)
                             tcCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTaa                                                                                                  >  2:672438/1‑140 (MQ=255)
                             tcCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTaa                                                                                                  >  2:286411/1‑140 (MQ=255)
                               cGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGc                                                                                                >  1:836050/1‑140 (MQ=255)
                                  cTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCaa                                                                                             <  2:406879/140‑1 (MQ=255)
                                     aaaCGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATCCCAAACTGGTCAGCCACCGAGAAAATTTCCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACgg                                                                                          <  1:401648/140‑1 (MQ=255)
                                          gACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCa                                                                                     <  1:755882/140‑1 (MQ=255)
                                            cGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATg                                                                                   >  2:30980/1‑140 (MQ=255)
                                            cGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATg                                                                                   >  2:607008/1‑140 (MQ=255)
                                              cAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGa                                                                                 <  1:643379/140‑1 (MQ=255)
                                              cAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGa                                                                                 <  2:508389/140‑1 (MQ=255)
                                                aaTCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCa                                                                                     <  2:230515/134‑1 (MQ=255)
                                                aaTCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCa                                                                                     >  1:230515/1‑134 (MQ=255)
                                                  tCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAg                                                                             <  1:672438/140‑1 (MQ=255)
                                                       aaTACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTgg                                                                        >  2:234124/1‑140 (MQ=255)
                                                        aTACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGc                                                                       <  1:553078/140‑1 (MQ=255)
                                                        aTACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGc                                                                       <  1:234124/140‑1 (MQ=255)
                                                         tACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGcc                                                                      <  1:463675/140‑1 (MQ=255)
                                                           cGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTg                                                                    >  2:679313/1‑140 (MQ=255)
                                                             ttCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGc                                                                  <  1:105680/140‑1 (MQ=255)
                                                              tCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCt                                                                 >  1:710671/1‑140 (MQ=255)
                                                               cGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCtt                                                                >  2:449466/1‑140 (MQ=255)
                                                                 ccGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTAc                                                              >  2:20805/1‑140 (MQ=255)
                                                                  cGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTAcc                                                             <  2:828650/140‑1 (MQ=255)
                                                                      acCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGt                                                         <  1:607008/140‑1 (MQ=255)
                                                                            gTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGcc                                                   <  1:679313/140‑1 (MQ=255)
                                                                            gTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGcc                                                   <  1:408133/140‑1 (MQ=255)
                                                                            gTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGcc                                                   <  1:39049/140‑1 (MQ=255)
                                                                            gTAGCGGATAGGGATACCAAACTGGTCAACCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGcc                                                   <  2:859399/140‑1 (MQ=255)
                                                                                cGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGc                                                    <  1:279665/135‑1 (MQ=255)
                                                                                cGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGc                                                    >  2:279665/1‑135 (MQ=255)
                                                                                 ggATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTg                                              <  2:348765/140‑1 (MQ=255)
                                                                                 ggATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTg                                              <  2:305539/140‑1 (MQ=255)
                                                                                    tAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTg                                           >  1:200693/1‑140 (MQ=255)
                                                                                     aGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGcc                                                   <  2:917745/131‑1 (MQ=255)
                                                                                     aGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGcc                                                   >  1:917745/1‑131 (MQ=255)
                                                                                     aGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTg                                              <  2:833927/136‑1 (MQ=255)
                                                                                     aGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTg                                              >  1:833927/1‑136 (MQ=255)
                                                                                     aGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTgg                                          <  1:370506/140‑1 (MQ=255)
                                                                                         aTACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATc                                      >  2:154552/1‑140 (MQ=255)
                                                                                           aCCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCg                                                 <  1:857772/127‑1 (MQ=255)
                                                                                           aCCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCg                                                 >  2:857772/1‑127 (MQ=255)
                                                                                            ccAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCt                                            <  2:858114/131‑1 (MQ=255)
                                                                                            ccAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCt                                            >  1:858114/1‑131 (MQ=255)
                                                                                             cAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATa                                  <  1:154552/140‑1 (MQ=255)
                                                                                                aCTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTc                               >  2:787387/1‑140 (MQ=255)
                                                                                                 cTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATa                                  <  2:56488/136‑1 (MQ=255)
                                                                                                 cTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGt                                >  2:19377/1‑138 (MQ=255)
                                                                                                 cTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGt                                <  1:19377/138‑1 (MQ=255)
                                                                                                 cTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCACGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATa                                  >  1:56488/1‑136 (MQ=255)
                                                                                                    gTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCa                           >  1:716422/1‑140 (MQ=255)
                                                                                                                   aaTTACCCCGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATgcggcg            >  1:128196/1‑140 (MQ=255)
                                                                                                                         cccGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCaa      <  2:515779/140‑1 (MQ=255)
                                                                                                                           cGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTAATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATg                 <  1:882441/127‑1 (MQ=255)
                                                                                                                           cGCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTAATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATg                 >  2:882441/1‑127 (MQ=255)
                                                                                                                            gCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGt   <  1:449466/140‑1 (MQ=255)
                                                                                                                            gCCTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGt   <  1:286411/140‑1 (MQ=255)
                                                                                                                             ccTTTCGCCGCGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTc  >  1:527558/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                 
CTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTC  >  NC_000913/3602758‑3603022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A