Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F28 I1 R1 227 52.0 1764248 99.3% 1751898 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,605,983 A→G Y78C (TAC→TGC)  yhhN → conserved inner membrane enzyme YhhN

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,605,9830AG100.0% 199.8 / NA 57Y78C (TAC→TGC) yhhNconserved inner membrane enzyme YhhN
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/30);  total (27/30)

TAACCCTTCTTCTCCTGCTGTTACTGGCCTGGCAAGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACG‑‑CTCTCTTTCTTCTGGCCTCTACCACTGGTGCTGCTGGTTCTGGGTGCGCTGTTACTGGCGATTATCTGGAC  >  NC_000913/3605848‑3606118
                                                                                                                                       |                                                                                                                                         
tAACCCTTCTTCTCCTGCTGTTACTGGCCTGGCAAGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTGCGcc                                                                                                                                       >  2:446394/1‑140 (MQ=255)
tAACCCTTCTTCTCCTGCTGTTACTGGCCTGGCAAGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTGCGcc                                                                                                                                       >  2:282613/1‑140 (MQ=255)
 aaCCCTTCTTCTCATGCTGTTACTGGCCTGGCAAGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTGCGCCa                                                                                                                                      >  1:802262/1‑140 (MQ=255)
       tcttctCCTGCTGTTACTGGCCTGGCAAGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTGCGCCATCGgcg                                                                                                                                >  2:523126/1‑140 (MQ=255)
                 ctgTTACTGGCCTGGCAAGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTGCGCCATCGGCGCGTTTTTCct                                                                                                                      <  2:230499/140‑1 (MQ=255)
                    ttACTGGCCTGGCAAGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCGCGTCAACGTCTGATGTGCGCCATCGgc                                                                                                                                 <  1:778628/126‑1 (MQ=255)
                    ttACTGGCCTGGCAAGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCGCGTCAACGTCTGATGTGCGCCATCGgc                                                                                                                                 >  2:778628/1‑126 (MQ=255)
                     tACTGGCCTGGCAAGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTGCGCCATCGGCGCGTTTTTCCTCTCg                                                                                                                  >  2:116766/1‑140 (MQ=255)
                      aCTGGCCTGGCAAGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTGCGCCATCGGCGCGTTTTTCCTCTCGc                                                                                                                 >  1:737632/1‑140 (MQ=255)
                      aCTGGCCTGGCAAGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTGCGCCATCGGCGCGTTTTTCCTCTCGc                                                                                                                 <  1:126558/140‑1 (MQ=255)
                        tggcctggcAAGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTGCGCCATCGGCGCGTTTTTCCTCTCGCAc                                                                                                               <  1:282613/140‑1 (MQ=255)
                        tggcctggcAAGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTGCGCCATCGGCGCGTTTTTCCTCTCGCAc                                                                                                               <  2:283945/140‑1 (MQ=255)
                        tggcctggcAAGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTGCGCCATCGGCGCGTTTTTCCTCTCGCAc                                                                                                               <  2:22546/140‑1 (MQ=255)
                        tggcctggcAAGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTGCGCCATCGGCGCGTTTTTCCTCTCGCAc                                                                                                               <  2:789327/140‑1 (MQ=255)
                         ggcctggcAAGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTGCGCCATCGGCGCGTTTTTCCTCTCGCAcc                                                                                                              <  1:446394/140‑1 (MQ=255)
                          gcctggcAAGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTGCGCCATCGGCGCGTTTTTCCTCTCGCACct                                                                                                             >  1:187840/1‑140 (MQ=255)
                            ctggcAAGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTGCGCCATCGGCGCGTTTTTCCTCTCGc                                                                                                                 <  1:116579/134‑1 (MQ=255)
                            ctggcAAGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTGCGCCATCGGCGCGTTTTTCCTCTCGc                                                                                                                 >  2:116579/1‑134 (MQ=255)
                             tggcAAGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTGCGCCATCGGCGCGTTTTTCCTCTCGCACctgct                                                                                                          <  1:523126/140‑1 (MQ=255)
                              ggcAAGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTGCGCCATCGGCGCGTTTTTCCTCTCGCACctgctg                                                                                                         >  1:318975/1‑140 (MQ=255)
                              ggcAAGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTGCGCCATCGGCGCGTTTTTCCTCTCGCACctgctg                                                                                                         <  1:395854/140‑1 (MQ=255)
                              gacAAGCGCCAATGTTCAACGCCATTAGCTATCTGGGTCTGGCAGGGCTGTGGGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACctgctg                                                                                                         >  2:486753/3‑140 (MQ=255)
                                                      ttAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTGCGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAg                                                                                 >  1:762203/1‑140 (MQ=255)
                                                       tAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTGCGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGa                                                                                <  2:623682/140‑1 (MQ=255)
                                                        aGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTGCGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGAt                                                                               <  1:292096/140‑1 (MQ=255)
                                                             tctcGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTGCGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGACAGATGACG‑‑c                                                                        <  2:724180/140‑1 (MQ=255)
                                                             tCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTGCGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCg                                                                                         <  1:207383/125‑1 (MQ=255)
                                                             tCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTGCGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCg                                                                                         >  2:207383/1‑125 (MQ=255)
                                                              cTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTGCGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACG‑‑ct                                                                       <  2:39734/140‑1 (MQ=255)
                                                              cTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTGCGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACG‑‑c                                                                        <  1:664504/139‑1 (MQ=255)
                                                              cTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTGCGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACG‑‑c                                                                        >  2:664504/1‑139 (MQ=255)
                                                               tGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTGCGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACG‑‑ctc                                                                      <  1:116766/140‑1 (MQ=255)
                                                                ggTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTGCGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACG‑‑ctct                                                                     >  2:749512/1‑140 (MQ=255)
                                                                  tGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTGCGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACG‑‑ctctct                                                                   <  2:187840/140‑1 (MQ=255)
                                                                  tGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTGCGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACG‑‑ctctct                                                                   <  1:715861/140‑1 (MQ=255)
                                                                  tGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTGCGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACG‑‑ctctct                                                                   >  1:482743/1‑140 (MQ=255)
                                                                       gCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTGCGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACG‑‑CTCtctttctt                                                              >  2:792714/1‑140 (MQ=255)
                                                                       gCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTGCGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACG‑‑CTCtctttctt                                                              <  1:792714/140‑1 (MQ=255)
                                                                            gCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTGCGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACG‑‑CTCTCTTTCTTCTGGc                                                         >  2:185655/1‑140 (MQ=255)
                                                                                tgCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTGCGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACG‑‑CTCTCTTTCTTCTGGCctct                                                     >  1:609293/1‑140 (MQ=255)
                                                                                 gcgcCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTGCGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACG‑‑CTCTCTTTCTTCTGGCCTCTa                                                    >  1:393805/1‑140 (MQ=255)
                                                                                   gcCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTGCGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACG‑‑CTCTCTTTCTTCTGGCCTCTAcc                                                  <  2:393805/140‑1 (MQ=255)
                                                                                     cTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTGCGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACG‑‑CTCtctttctt                                                              >  1:59870/1‑126 (MQ=255)
                                                                                     cTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTGCGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACG‑‑CTCtctttctt                                                              <  2:59870/126‑1 (MQ=255)
                                                                                     cTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTGCGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACG‑‑CTCTCTTTCTTCTgg                                                          >  2:149194/1‑130 (MQ=255)
                                                                                     cTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTGCGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACG‑‑CTCTCTTTCTTCTgg                                                          <  1:149194/130‑1 (MQ=255)
                                                                                         ctgctgGGCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTGCGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACG‑‑CTCTCTTTCTTCTGGCCTCTACCACTGGt                                            <  2:762203/140‑1 (MQ=255)
                                                                                               ggCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTGCGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACG‑‑CTCTCTTTCTTCTGGCCTCTACCACTGGTgctgct                                      <  2:737632/140‑1 (MQ=255)
                                                                                                    tGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTGCGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACG‑‑CTCTCTTTCTTCTGGCCTCTACCACTGGTGCTGCTGGTTc                                 >  1:527040/1‑140 (MQ=255)
                                                                                                    tGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTGCGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACG‑‑CTCTCTTTCTTCTGGCCTCTACCACTGGTGCTGCTGGTTc                                 >  1:433165/1‑140 (MQ=255)
                                                                                                          aaCCCTGTTGCCACGTCAACGTCTGATGTGCGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACG‑‑CTCTCTTTCTTCTGGCCTCTACCACTGGTGCTGCTGGTTCTGGGTg                           <  2:482743/140‑1 (MQ=255)
                                                                                                          aaCCCTGTTGCCACGTCAACGTCTGATGTGCGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACG‑‑CTCTCTTTCTTCTGGCCTCTACCACTGGTGCTGCTGGTTCTGGGTg                           <  2:159705/140‑1 (MQ=255)
                                                                                                                gTTGCCACGTCAACGTCTGATGTGCGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACG‑‑CTCTCTTTCTTCTGGCCTCTACCACTGGTGCTGCTGGTTCTGGGTGCGCTGt                     >  1:456626/1‑140 (MQ=255)
                                                                                                                  tGCCACGTCAACGTCTGATGTGCGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACG‑‑CTCTCTTTCTTCTGGCCTCTACCACTGGTGCTGCTGGTTCTGGGTGCGCTGTTa                   >  2:151439/1‑140 (MQ=255)
                                                                                                                   gCCACGTCAACGTCTGATGTGCGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACG‑‑CTCTCTTTCTTCTGGCCTCTACCACTGGTGCTGCTGGTTCTGGGTGCGCTGTTAc                  <  1:185655/140‑1 (MQ=255)
                                                                                                                         tCAACGTCTGATGTGCGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACG‑‑CTCTCTTTCTTCTGGCCTCTACCACTGGTGCTGCTGGTTCTGGGTGCGCTGTTACTGGCGa            >  2:830532/1‑140 (MQ=255)
                                                                                                                                   aTGTGCGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGCTCTCTCTTTCTTCTGGCCTCTACCACTGGTGCTGCTGGTTCTGGGTGCGCTGTTACTGGCGATTATCTgg    >  2:341180/1‑140 (MQ=255)
                                                                                                                                   aTGTGCGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACG‑‑CTCTCTTTCTTCTGGCCTCTACCACTGGTGCTGCTGGTTCTGGGTGCGCTGTTACTGGCGATTATCTGGAc  <  2:802262/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                         
TAACCCTTCTTCTCCTGCTGTTACTGGCCTGGCAAGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACG‑‑CTCTCTTTCTTCTGGCCTCTACCACTGGTGCTGCTGGTTCTGGGTGCGCTGTTACTGGCGATTATCTGGAC  >  NC_000913/3605848‑3606118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A