Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F28 I1 R1 155 60.3 2039550 99.3% 2025273 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,632,011 T→C T194A (ACA→GCA)  yhiJ ← DUF4049 domain‑containing protein YhiJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,632,0110TC98.8% 289.0 / ‑4.5 83T194A (ACA→GCA) yhiJDUF4049 domain‑containing protein YhiJ
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (39/43);  total (40/43)
Fisher's exact test for biased strand distribution p-value = 4.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

TCTATATTAAGCAACTGGCTAAGGAAATTTTCTCCTTCAGCCATGATTCCCAAATGTCCCGTTGCGAAAAGCGCGAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGTGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGTTTTATCATTGTTTCGAATTTAATATCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAA  >  NC_000913/3631878‑3632145
                                                                                                                                     |                                                                                                                                      
tctATATTAAGCAACTGGCTAAGGAAATTTTCTCCTTCAGCCATGATTCCCAAATGTCCCGTTGCGAAAAGCGCGAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATc                                                                                                                                  >  2:824960/1‑140 (MQ=255)
   atatTAAGCAACTGGCTAAGGAAATTTTCTCCTTCAGCCATGATTCCCAAATGTCCCGTTGCGAAAAGCGCGAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAAc                                                                                                                               <  2:879506/140‑1 (MQ=255)
       tAAGCAACTGGCTAAGGAAATTTTCTCCTTCAGCCATGATTCCCAAATGTCCCGTTGCGAAAAGCGCGAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGt                                                                                                                           >  2:990309/1‑140 (MQ=255)
         aGCAACTGGCTAAGGAAATTTTCTCCTTCAGCCATGATTCCCAAATGTCCCGTTGCGAAAAGCGCGAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTaa                                                                                                                         >  2:407107/1‑140 (MQ=255)
         aGCAACTGGCTAAGGAAATTTTCTCCTTCAGCCATGATTCCCAAATGTCCCGTTGCGAAAAGCGCGAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTaa                                                                                                                         >  1:660566/1‑140 (MQ=255)
         aGCAACTGGCTAAGGAAATTTTCTCCTTCAGCCATGATTCCCAAATGTCCCGTTGCGAAAAGCGCGAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTaa                                                                                                                         >  1:11052/1‑140 (MQ=255)
         aGCAACTGGCTAAGGAAATTTTCTCCTTCAGCCATGATTCCCAAATGTCCCGTTGCGAAAAGCGCGAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTaa                                                                                                                         >  1:997251/1‑140 (MQ=255)
          gCAACTGGCTAAGGAAATTTTCTCCTTCAGCCATGATTCCCAAATGTCCCGTTGCGAAAAGCGCGAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTaaa                                                                                                                        <  2:997251/140‑1 (MQ=255)
           cAACTGGCTAAGGAAATTTTCTCCTTCAGCCATGATTCCCAAATGTCCCGTTGCGAAAAGCGCGAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAAc                                                                                                                       >  1:247978/1‑140 (MQ=255)
              cTGGCTAAGGAAATTTTCTCCTTCAGCCATGATTCCCAAATGTCCCGTTGCGAAAAGCGCGAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACa                                                                                                                      <  2:820982/138‑1 (MQ=255)
              cTGGCTAAGGAAATTTTCTCCTTCAGCCATGATTCCCAAATGTCCCGTTGCGAAAAGCGCGAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACa                                                                                                                      >  1:820982/1‑138 (MQ=255)
              cTGGCTAAGGAAATTTTCTCCTTCAGCCATGATTCCCAAATGTCCCGTTGCGAAAAGCGCGAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAAt                                                                                                                    >  2:276037/1‑140 (MQ=255)
              cTGGCTAAGGAAATTTTCTCCTTCAGCCATGATTCCCAAATGTCCCGTTGCGAAAAGCGCGAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAAt                                                                                                                    >  2:218415/1‑140 (MQ=255)
              cTGGCTAAGGAAATTTTCTCCTTCAGCCATGATTCCCAAATGTCCCGTTGCGAAAAGCGCGAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAAt                                                                                                                    >  2:12513/1‑140 (MQ=255)
              cTGGCTAAGGAAATTTTCTCCTTCAGCCATGATTCCCAAATGTCCCGTTGCGAAAAGCGCGAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAAt                                                                                                                    >  1:194456/1‑140 (MQ=255)
              cTGGCTAAGGAAATTTTCTCCTTCAGCCATGATTCCCAAATGTCCCGTTGCGAAAAGCGCGAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAAt                                                                                                                    >  1:663525/1‑140 (MQ=255)
              cTGGCTAAGGAAATTTTCTCCTTCAGCCATGATTCCCAAATGTCCCGTTGCGAAAAGCGCGAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAAt                                                                                                                    >  1:510718/1‑140 (MQ=255)
                ggCTAAGGAAATTTTCTCCTTCAGCCATGATTCCCAAATGTCCCGTTGCGAAAAGCGCGAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAAt                                                                                                                    >  1:910000/1‑138 (MQ=255)
                ggCTAAGGAAATTTTCTCCTTCAGCCATGATTCCCAAATGTCCCGTTGCGAAAAGCGCGAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAAt                                                                                                                    <  2:910000/138‑1 (MQ=255)
                ggCTAAGGAAATTTTCTCCTTCAGCCATGATTCCCAAATGTCCCGTTGCGAAAAGCGCGAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTg                                                                                                                  <  1:824960/140‑1 (MQ=255)
                ggCTAAGGAAATTTTCTCCTTCAGCCATGATTCCCAAATGTCCCGTTGCGAAAAGCGCGAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTg                                                                                                                  >  1:519742/1‑140 (MQ=255)
                ggCTAAGGAAATTTTCTCCTTCAGCCATGATTCCCAAATGTCCCGTTGCGAAAAGCGCGAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTg                                                                                                                  <  1:158557/140‑1 (MQ=255)
                  cTAAGGAAATTTTCTCCTTCAGCCATGATTCCCAAATGTCCCGTTGCGAAAAGCGCGAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGga                                                                                                                <  1:850710/140‑1 (MQ=255)
                           ttttCTCCTTCAGCCATGATTCCCAAATGTCCCGTTGCGAAAAGCGCGAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGAt                                                                                                       >  2:739035/1‑140 (MQ=255)
                             ttCTCCTTCAGCCATGATTCCCAAATGTCCCGTTGCGAAAAGCGCGAACAAAACTGCACCGACTGAGCCGTCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTc                                                                                                     <  2:14305/140‑1 (MQ=255)
                                    tCAGCCATGATTCCCAAATGTCCCGTTGCGAAAAGCGCGAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGc                                                                                              <  1:374172/140‑1 (MQ=255)
                                       gCCATGATTCCCAAATGTCCCGTTGCGAAAAGCGCGAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGaa                                                                                           <  1:54837/140‑1 (MQ=255)
                                       gCCATGATTCCCAAATGTCCCGTTGCGAAAAGCGCGAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGaa                                                                                           <  1:464094/140‑1 (MQ=255)
                                        ccATGATTCCCAAATGTCCCGTTGCGAAAAGCGCGAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAAc                                                                                          <  1:407107/140‑1 (MQ=255)
                                          aTGATTCCCAAATGTCCCGTTGCGAAAAGCGCGAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTg                                                                                        >  2:937055/1‑140 (MQ=255)
                                          aTGATTCCCAAATGTCCCGTTGCGAAAAGCGCGAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTg                                                                                        <  1:12513/140‑1 (MQ=255)
                                           tGATTCCCAAATGTCCCGTTGCGAAAAGCGCGAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTgg                                                                                       <  1:900076/140‑1 (MQ=255)
                                                cccAAATGTCCCGTTGCGAAAAGCGCGAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGttt                                                                                  <  2:194456/140‑1 (MQ=255)
                                                cccAAATGTCCCGTTGCGAAAAGCGCGAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGttt                                                                                  <  1:773639/140‑1 (MQ=255)
                                                     aTGTCCCGTTGCGAAAAGCGCGAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGTTTTATCa                                                                             <  1:990309/140‑1 (MQ=255)
                                                       gTCCCGTTGCGAAAAGCGCGAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGTTTTATCAtt                                                                           >  2:426437/1‑140 (MQ=255)
                                                        tCCCGTTGCGAAAAGCGCGAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGTTTTATCATTg                                                                          >  2:549557/1‑140 (MQ=255)
                                                           cGTTGCGAAAAGCGCGAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGTTTTATCATTGttt                                                                       >  1:115475/1‑140 (MQ=255)
                                                            gTTGCGAAAAGCGCGAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGTTTTATCATTGTTTc                                                                      <  2:510718/140‑1 (MQ=255)
                                                            gTTGCGAAAAGCGCGAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGTTTTATCATTGTTTc                                                                      >  1:270567/1‑140 (MQ=255)
                                                            gTTGCGAAAAGCGCGAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGTTTTATCATTGTTTc                                                                      <  2:115475/140‑1 (MQ=255)
                                                            gTTGCGAAAAGCGCGAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGTTTTATCATTGTTTc                                                                      >  2:274840/1‑140 (MQ=255)
                                                             ttGCGAAAAGCGCGAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGTTTTATCATTGTTTCg                                                                     >  2:135444/1‑140 (MQ=255)
                                                             ttGCGAAAAGCGCGAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGTTTTATCATTGTTTCg                                                                     <  1:74285/140‑1 (MQ=255)
                                                               gCGAAAAGCGCGAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGTTTTATCATTGTTTCGaa                                                                   >  2:837967/1‑140 (MQ=255)
                                                                cGAAAAGCGCGAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGTTTTATCATTGTTTCGAAt                                                                  <  2:637911/140‑1 (MQ=255)
                                                                 gAAAAGCGCGAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGTTTTATCATTGTTTCGAAtt                                                                 <  1:426437/140‑1 (MQ=255)
                                                                    aaGCGCGAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGTTTTATCATTGTTTCGAATTTaa                                                              <  1:274840/140‑1 (MQ=255)
                                                                        gcgAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGTTTTATCATTGTTTCGAATTTAATATc                                                          <  2:674548/140‑1 (MQ=255)
                                                                        gcgAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGTTTTATCATTGTTTCGAATTTAATATc                                                          <  1:135444/140‑1 (MQ=255)
                                                                          gAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGTTTTATCATTGTTTCGAATTTAATATCAg                                                        <  1:675565/140‑1 (MQ=255)
                                                                          gAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGTTTTATCATTGTTTCGAATTTAATATCAg                                                        <  2:617646/140‑1 (MQ=255)
                                                                          gAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGTTTTATCATTGTTTCGAATTTAATATCAg                                                        >  1:733762/1‑140 (MQ=255)
                                                                          gAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGTTTTATCATTGTTTCGAATTTAATATCAg                                                        <  2:270567/140‑1 (MQ=255)
                                                                          gAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGTTTTATCATTGTTTCGAATTTAATATCAg                                                        <  1:460311/140‑1 (MQ=255)
                                                                          gAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGTTTTATCATTGTTTCGAATTTAATATCAg                                                        <  2:933660/140‑1 (MQ=255)
                                                                            aCAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGTTTTATCATTGTTTCGAATTTAATATCAGAt                                                      <  1:739035/140‑1 (MQ=255)
                                                                            aCAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGTTTTATCATTGTTTCGAATTTAATATCAGAt                                                      <  2:11052/140‑1 (MQ=255)
                                                                               aaaCTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGTTTTATCATTGTTTCGAATTTAATATCAGATACa                                                   <  1:837967/140‑1 (MQ=255)
                                                                                     cACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGTTTTATCATTGTTTCGAATTTAATATCAGAt                                                      >  2:446186/1‑131 (MQ=255)
                                                                                     cACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACCGGGGTTTTATCATTGTTTCGAATTTAATATCAGAt                                                      <  1:446186/131‑1 (MQ=255)
                                                                                       ccGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGTTTTATCATTGTTTCGAATTTAATATCAGATACATTATATTg                                           <  1:846570/140‑1 (MQ=255)
                                                                                            tGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGTTTTATCATTGTTTCGAATTTAATATCAGATACATTATATTGCGata                                      <  2:733762/140‑1 (MQ=255)
                                                                                             gAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGTTTTATCATTGTTTCGAATTTAATATCAGATACATTATATTGCGatat                                     >  1:14794/1‑140 (MQ=255)
                                                                                                ccATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGTTTTATCATTGTTTCGAATTTAATATCAGATACATTATATTGCGATAtttt                                  >  2:852781/1‑140 (MQ=255)
                                                                                                  aTCGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGTTTTATCATTGTTTCGAATTTAATATCAGATACATTATATTGCGATATTttcc                                >  1:891968/1‑140 (MQ=255)
                                                                                                    cGGTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGTTTTATCATTGTTTCGAATTTAATATCAGATACATTATATTGCGATATTttccag                              >  2:286953/1‑140 (MQ=255)
                                                                                                     ggTATCACCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGCAATAGATTCTGTCAGCGAACTGGGGTTTTATCATTGTTTCGAATTTAATATCAGATACATTATATTGCGATATTttccagt                             <  2:891968/140‑1 (MQ=255)
                                                                                                           aCCAAAATAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGTTTTATCATTGTTTCGAATTTAATATCAGATACATTATATTGCGATATTTTCCAGTTCCAGa                       <  2:519742/140‑1 (MQ=255)
                                                                                                                aaTAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGTTTTATCATTGTTTCGAATTTAATATCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGt                  <  1:695577/140‑1 (MQ=255)
                                                                                                                 aTAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGTTTTATCATTGTTTCGAATTTAATATCAGATACATTATATTGCGATATTTTCCAGTTCCAGa                       <  1:73296/134‑1 (MQ=255)
                                                                                                                 aTAGGTTGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGTTTTATCATTGTTTCGAATTTAATATCAGATACATTATATTGCGATATTTTCCAGTTCCAGa                       >  2:73296/1‑134 (MQ=255)
                                                                                                                      ttGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGTTTTATCATTGTTTCGAATTTAATATCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTAc            <  1:937055/140‑1 (MQ=255)
                                                                                                                       tGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGTTTTATCATTGTTTCGAATTTAATATCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACg           >  2:395701/1‑140 (MQ=255)
                                                                                                                       tGGTCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGTTTTATCATTGTTTCGAATTTAATATCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACg           >  1:7479/1‑140 (MQ=255)
                                                                                                                          tCTGGTACTTGTGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGTTTTATCATTGTTTCGAATTTAATATCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACt        >  1:849885/1‑140 (MQ=255)
                                                                                                                          tCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGTTTTATCATTGTTTCGAATTTAATATCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACt        >  1:250440/1‑140 (MQ=255)
                                                                                                                          tCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGTTTTATCATTGTTTCGAATTTAATATCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACt        <  2:14794/140‑1 (MQ=255)
                                                                                                                          tCTGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGTTTTATCATTGTTTCGAATTTAATATCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACt        >  1:641729/1‑140 (MQ=255)
                                                                                                                            tGGTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGTTTTATCATTGTTTCGAATTTAATATCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCa      >  2:552480/1‑140 (MQ=255)
                                                                                                                              gTACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGTTTTATCATTGTTTCGAATTTAATATCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGt    <  1:587828/140‑1 (MQ=255)
                                                                                                                               tACTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGTTTTATCATTGTTTCGAATTTAATATCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTa   >  1:691539/1‑140 (MQ=255)
                                                                                                                                aCTTGCGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGTTTTATCATTGTTTCGAATTTAATATCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTaa  >  2:951723/1‑140 (MQ=255)
                                                                                                                                     |                                                                                                                                      
TCTATATTAAGCAACTGGCTAAGGAAATTTTCTCCTTCAGCCATGATTCCCAAATGTCCCGTTGCGAAAAGCGCGAACAAAACTGCACCGACTGAGCCATCGGTATCACCAAAATAGGTTGGTCTGGTACTTGTGGGATCAACGGGTAAACAATTGGAGAAATAGATTCTGTCAGCGAACTGGGGTTTTATCATTGTTTCGAATTTAATATCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAA  >  NC_000913/3631878‑3632145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A