Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	155	60.3	2039550	99.3%	2025273	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,717,584	T→C	L92P (CTG→CCG)	ghrB →	glyoxylate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,717,584	0	T	C	100.0%	211.5 / NA	59	L92P (CTG→CCG)	ghrB	glyoxylate reductase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (26/33); total (26/33)

GGTTTACTGGGTTCAAACGAGAATGTAAATGCCGCATTGCTGGAAAAAATGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCTGATGCACACGCCAACCGTATTAACAGAAACCGTCGCCGATACGCTGATGGCGCTGGTGTTGTCTACCGCTCGTCGGGTTGTGGAGGTAGCAGAACGGGTAAAAGCAGGCGAATGGACCGCGAGCATAGGCCCG  >  NC_000913/3717451‑3717717

ggTTTACTGGGTTCAAACGAGAATGTAAATGCCGCATTGCTGGAAAAAATGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGca                                                                                                                                 <  1:202830/140‑1 (MQ=255)

      cTGGGTTCAAACGAGAATGTAAATGCCGCATTGCTGGAAAAAATGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGCACACGcc                                                                                                                           <  1:21759/140‑1 (MQ=255)

        gggTTCAAACGAGAATGTAAATGCCGCATTGCTGGAAAAAATGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGCACACGCCaa                                                                                                                         >  2:540337/1‑140 (MQ=255)

        gggTTCAAACGAGAATGTAAATGCCGCATTGCTGGAAAAAATGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGCACACGCCaa                                                                                                                         >  1:566295/1‑140 (MQ=255)

         ggTTCAAACGAGAATGTAAATGCCGCATTGCTGGAAAAAATGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGCACACGCCAAc                                                                                                                        <  1:970384/140‑1 (MQ=255)

              aaaCGAGAATGTAAATGCCGCATTGCTGGAAAAAATGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGCACACGCCAACCGTAt                                                                                                                   <  1:758929/140‑1 (MQ=255)

               aaCGAGAATGTAAATGCCGCATTGCTGGAAAAAATGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGCACACGCCAACCGTAtt                                                                                                                  <  1:480939/140‑1 (MQ=255)

                   agaATGTAAATGCCGCATTGCTGGAAAAAATGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGCACACGCCAACCGTATTAACa                                                                                                              <  2:782222/140‑1 (MQ=255)

                     aaTGTAAATACCGCATTGCTGGAAAAAATGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGCACACGCCAACCGTATCAACAGa                                                                                                            >  2:859172/1‑140 (MQ=255)

                               ccGCATTGCTGGAAAAAATGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGCACACGCCAACCGTATTAACAGAAACCGTCGcc                                                                                                  >  1:426970/1‑140 (MQ=255)

                               ccGCATTGCTGGAAAAAATGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGCACACGCCAACCGTATTAACAGAAACCGTCGcc                                                                                                  >  1:915251/1‑140 (MQ=255)

                                  cATTGCTGGAAAAAATGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGCACACGCCAACCGTATTAACAGAAACCGTCGCCGAt                                                                                               >  2:796884/1‑140 (MQ=255)

                                  cATTGCTGGAAAAAATGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGCACACGCCAACCGTATTAACAGAAACCGTCGCCGAt                                                                                               >  1:579673/1‑140 (MQ=255)

                                   aTTGCTGGAAAAAATGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGCACACGCCAACCGTATTAACAGAAACCGTCGCCGATa                                                                                              <  1:77469/140‑1 (MQ=255)

                                       cTGGAAAAAATGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGCACACGCCAACCGTATTAACAGAAACCGTCGcc                                                                                                  <  1:294220/132‑1 (MQ=255)

                                       cTGGAAAAAATGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGCACACGCCAACCGTATTAACAGAAACCGTCGcc                                                                                                  >  2:294220/1‑132 (MQ=255)

                                          gAAAAAATGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTTGAGGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGCACACGCCAACCGTATTAACAGAAACCGTCGCCGATACGCTGAt                                                                                       <  2:6519/140‑1 (MQ=255)

                                             aaaaTGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGCACACGCCAACCGTATTAACAGAAACCGTCGCCGATACGCTGATGgc                                                                                    <  2:99222/140‑1 (MQ=255)

                                              aaaTGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGCACACGCCAACCGTATTAACAGAAACCGTCGCCGATACGCTGATGgcg                                                                                   <  2:902593/140‑1 (MQ=255)

                                              aaaTGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGCACACGCCAACCGTATTAACAGAAACCGTCGCCGATACGCTGATGgcg                                                                                   <  2:566295/140‑1 (MQ=255)

                                               aaTGCCGAAACTGCGTGCCACATCAGCGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGCACACGCCAACCGTATTAACAGAAACCGTCGCCGATACGCTGATGgcgc                                                                                  <  2:458417/140‑1 (MQ=255)

                                               aaTGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGCACACGCCAACCGTATTAACAGAAACCGTCGCCGATACGCTGATGgcgc                                                                                  >  2:848027/1‑140 (MQ=255)

                                                 tGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGCACACGCCAACCGTATTAACAGAAACCGTCGCCGATACGCTGATGGCGCTg                                                                                <  2:915251/140‑1 (MQ=255)

                                                 tGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGCACACGCCAACCGTATCAACAGAAACCGTCGCCGATACGCTGATGGCGCTg                                                                                <  1:859172/140‑1 (MQ=255)

                                                       aaCTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGCACACGCCAACCGTATTAACAGAAACCGTCGCCGATACGCTGATGGCGCTGgtg                                                                             <  1:850043/137‑1 (MQ=255)

                                                       aaCTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGCACACGCCAACCGTATTAACAGAAACCGTCGCCGATACGCTGATGGCGCTGgtg                                                                             >  2:850043/1‑137 (MQ=255)

                                                            cGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGCACACGCCAACCGTATTAACAGAAACCGTCGCCGATACGCTGATGGCGCTGgtg                                                                             >  1:245007/1‑132 (MQ=255)

                                                            cGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGCACACGCCAACCGTATTAACAGAAACCGTCGCCGATACGCTGATGGCGCTGgtg                                                                             <  2:245007/132‑1 (MQ=255)

                                                              tGCCACATCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGCACACGCCAACCGTATTAACAGAAACCGTCGCCGATACGCTGATGGCGCTGGTGTTGTCTACCg                                                                   >  2:578284/1‑140 (MQ=255)

                                                                  acaTCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGCACACGCCAACCGTATTAACAGAAACCGTCGCCGATACGCTGATGGCGCTGGTGTTGTCTACCGCtcg                                                               <  2:158292/140‑1 (MQ=255)

                                                                   caTCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGCACACGCCAACCGTATTAACAGAAACCGTCGCCGATACGCTGATGGCGCTGGTGTTGTCTACCGCtcgt                                                              <  1:796884/140‑1 (MQ=255)

                                                                    aTCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGCACACGCCAACCGTATTAACAGAAACCGTCGCCGATACGCTGATGGCGCTGGTGTTGTCTACCGCtcgtc                                                             >  2:469851/1‑140 (MQ=255)

                                                                    aTCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGCACACGCCAACCGTATTAACAGAAACCGTCGCCGATACGCTGATGGCGCTGGTGTTGTCTACCGCtcgtc                                                             >  1:266278/1‑140 (MQ=255)

                                                                    aTCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGCACACGCCAACCGTATTAACAGAAACCGTCGCCGATACGCTGATGGCGCTGGTGTTGTCTACCGCtcgtc                                                             >  2:760286/1‑140 (MQ=255)

                                                                    aTCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGCACACGCCAACCGTATTAACAGAAACCGTCGCCGATACGCTGATGGCGCTGGTGTTGTCTACCGCtcgtc                                                             >  2:132233/1‑140 (MQ=255)

                                                                        aCGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGCACACGCCAACCGTATTAACAGAAACCGTCGCCGATACGCTGATGGCGCTGGTGTTGTCTACCGCTCGTCGGGt                                                         <  1:540337/140‑1 (MQ=255)

                                                                             ctcCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCGGAAAAATTCTGCCGATGCACACGCCAACCGTATTAACAGAAACCGTCGCCGATACGCTGATGGCGCTGGTGTTGTCTAGCGCTCGTCGGGtt                                                        >  2:907376/1‑136 (MQ=255)

                                                                             ctcCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGCACACGCCAACCGTATTAACAGAAACCGTCGCCGATACGCTGATGGCGCTGGTGTTGTCTACCGCTCGTCGGGtt                                                        <  1:907376/136‑1 (MQ=255)

                                                                             ctcCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGCACACGCCAACCGTATTAACAGAAACCGTCGCCGATACGCTGATGGCGCTGGTGTTGTCTACCGCTCGTCGGGTTGTgg                                                    >  1:528812/1‑140 (MQ=255)

                                                                              tcCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGCACACGCCAACCGTATTAACAGAAACCGTCGCCGATACGCTGATGGCGCTGGTGTTGTCTACCGCTCGTCGGGTTGTGGa                                                   >  1:821434/1‑140 (MQ=255)

                                                                                 gTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGCACACGCCAACCGTATTAACAGAAACCGTCGCCGATACGCTGATGGCGCTGGTGTTGTCTACCGCTCGTCGGGTTGTGGAGGt                                                <  2:980660/140‑1 (MQ=255)

                                                                                   cGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGCACACGCCAACCGTATTAACAGAAACCGTCGCCGATACGCTGATGGCGCTGGTGTTGTCTACCGCTCGTCGGGTtgt                                                      <  1:783896/132‑1 (MQ=255)

                                                                                   cGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGCACACGCCAACCGTATTAACAGAAACCGTCGCCGATACGCTGATGGCGCTGGTGTTGTCTACCGCTCGTCGGGTtgt                                                      >  2:783896/1‑132 (MQ=255)

                                                                                        aTGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGCACACGCCAACCGTATTAACAGAAACCGTCGCCGATACGCTGATGGCGCTGGTGTTGTCTACCGCTCGTCGGGTTGTGGAGGTAGCAGaa                                         <  2:266278/140‑1 (MQ=255)

                                                                                        aTGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGCACACGCCAACCGTATTAACAGAAACCGTCGCCGATACGCTGATGGCGCTGGTGTTGTCTACCGCTCGTCGGGTTGTGGAGGTAGCAGaa                                         >  2:785348/1‑140 (MQ=255)

                                                                                          gACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGCACACGCCAACCGTATTAACAGAAACCGTCGCCGATACGCTGATGGCGCTGGTGTTGTCTACCGCTCGTCGGGTTGTGGAGGTAGCAGAACg                                       >  2:943155/1‑140 (MQ=255)

                                                                                               ttttGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGCACACGCCAACCGTATTAACAGAAACCGTCGCCGATACGCTGATGGCGCTGGTGTTGTCTACCGCTCGTCGGGTTGTGGAGGTAGCAGAACGGGTaa                                  >  2:175116/1‑140 (MQ=255)

                                                                                                tttGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGCACACGCCAACCGTATTAACAGAAACCGTCGCCGATACGCTGATGGCGCTGGTGTTGTCTACCGCTCGTCGGGTTGTGGAGGTAGCAGAACGGGTaaa                                 >  1:54667/1‑140 (MQ=255)

                                                                                                tttGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGCACACGCCAACCGTATTAACAGAAACCGTCGCCGATACGCTGATGGCGCTGGTGTTGTCTACCGCTCGTCGGGTTGTGGAGGTAGCAGAACGGGTaaa                                 <  2:605765/140‑1 (MQ=255)

                                                                                                tttGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGCACACGCCAACCGTATTAACAGAAACCGTCGCCGATACGCTGATGGCGCTGGTGTTGTCTACCGCTCGTCGGGTTGTGGAGGTAGCAGAACGGGTaaa                                 <  1:469851/140‑1 (MQ=255)

                                                                                                  tGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCCGATGCACACGCCAACCGTATTAACAGAAACCGTCGCCGATACGCTGATGGCGCTGGTGTTGTCTACCGCTCGTCGGGTTGTGGAGGTAGCAGAACGGGTAAAAg                               <  1:818881/140‑1 (MQ=255)

                                                                                                             cgcTTACCGCCCGAAAAATTCTGCCGATGCACACGCCAACCGTATTAACAGAAACCGTCGCCGATACGCTGATGGCGCTGGTGTTGTCTACCGCTCGTCGGGTTGTGGAGGTAGCAGAACGGGTAAAAGCAGGCGAATgg                    <  1:785348/140‑1 (MQ=255)

                                                                                                                  aCCGCCCGAAAAATTCTGCCGATGCACACGCCAACCGTATTAACAGAAACCGTCGCCGATACGCTGATGGCGCTGGTGTTGTCTACCGCTCGTCGGGTTGTGGAGGTAGCAGAACGGGTAAAAGCAGGCGAATGGACcgc               >  1:204359/1‑140 (MQ=255)

                                                                                                                       ccGAAAAATTCTGCCGATGCACACGCCAACCGTATTAACAGAAACCGTCGCCGATACGCTGATGGCGCTGGTGTTGTCTACCGCTCGTCGGGTTGTGGAGGTAGCAGAACGGGTAAAAGCAGGCGAATGGACCGCGAGCa          <  1:760286/140‑1 (MQ=255)

                                                                                                                       ccGAAAAATTCTGCCGATGCACACGCCAACCGTATTAACAGAAACCGTCGCCGATACGCTGATGGCGCTGGTGTTGTCTACCGCTCGTCGGGTTGTGGAGGTAGCAGAACGGGTAAAAGCAGGCGAATGGACCGCGAGCa          <  2:528812/140‑1 (MQ=255)

                                                                                                                        cGAAAAATTCTGCCGATGCACACGCCAACCGTATTAACAGAAACCGTCGCCGATACGCTGATGGCGCTGGTGTTGTCTACCGCTCGTCGGGTTGTGGAGGTAGCAGAACGGGTAAAAGCAGGCGAATGGACCGCGAGCAt         <  2:204359/140‑1 (MQ=255)

                                                                                                                          aaaaaTTCTGCCGATGCACACGCCAACCGTATTAACAGAAACCGTCGCCGATACGCTGATGGCGCTGGTGTTGTCTACCGCTCGTCGGGTTGTGGAGGTAGCAGAACGGGTAAAAGCAGGCGAATGGACCGCGAGCATAg       >  1:939325/1‑140 (MQ=255)

                                                                                                                             aaTTCTGCCGATGCACACGCCAACCGTATTAACAGAAACCGTCGCCGATACGCTGATGGCGCTGGTGTTGTCTACCGCTCGTCGGGTTGTGGAGGTAGCAGAACGGGTAAAAGCAGGCGAATGGACCGCGAGCATAGGcc    <  1:175116/140‑1 (MQ=255)

                                                                                                                               ttCTGCCGATGCACACGCCAACCGTATTAACAGAAACCGTCGCCGATACGCTGATGGCGCTGGTGTTGTCTACCGCTCGTCGGGTTGTGGAGGTAGCAGAACGGGTAAAAGCAGGCGAATGGACCGCGAGCATAGGCCCg  <  1:943155/140‑1 (MQ=255)

GGTTTACTGGGTTCAAACGAGAATGTAAATGCCGCATTGCTGGAAAAAATGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCTGATGCACACGCCAACCGTATTAACAGAAACCGTCGCCGATACGCTGATGGCGCTGGTGTTGTCTACCGCTCGTCGGGTTGTGGAGGTAGCAGAACGGGTAAAAGCAGGCGAATGGACCGCGAGCATAGGCCCG  >  NC_000913/3717451‑3717717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A