Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,718,895	T→C	E50E (GAA→GAG)	yiaF ←	DUF3053 domain‑containing protein YiaF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,718,895	0	T	C	100.0%	181.7 / NA	52	E50E (GAA→GAG)	yiaF	DUF3053 domain‑containing protein YiaF
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (29/23); total (29/23)

TAACGCTGTCTACAACCGGGCGCAGACCGGAATCCATCGCCTGATTCACCTGCTGAGAATAACCATACAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCTGATCGGCAGTCAGGGTTGGTAGACGTTCACCGCTACGCATCACTGTATTCTGCAGGAAGTCGATAAACGCTTTACGCTGATCGCCTTCTTTATCAAAACACCCACTCAGGCTAACTACCATTAATAACGCCGCAAGAGGCGCAAACCAGCGAGAGCAGG  >  NC_000913/3718759‑3719028

tAACGCTGTCTACAACCGGGCGCAGACCGGAATCCATCGCCTGATTCACCTGCTGAGAATAACCATACAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCTGATCGGCAGTCAGGGTTGGTAGACGCTCa                                                                                                                                    <  2:434761/140‑1 (MQ=255)

        tCTACAACCGGGCGCAGACCGGAATCCATCGCCTGATTCACCTGCTGAGAATAACCATACAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCTGATCGGCAGTCAGGGTTGGTAGACGCTCACCGCTACg                                                                                                                            <  1:737346/140‑1 (MQ=255)

          tACAACCGGGCGCAGACCGGAATCCATCGCCTGATTCACCTGCTGAGAATAACCATACAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCTGATCGGCAGTCAGGGTTGGTAGACGCTCACCGCTACGCa                                                                                                                          <  1:74249/140‑1 (MQ=255)

           aCAACCGGGCGCAGACCGGAATCCATCGCCTGATTCACCTGCTGAGAATAACCATACAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCTGATCGGCAGTCAGGGTTGGTAGACGCTCACCGCTACGCAt                                                                                                                         <  2:123149/140‑1 (MQ=255)

             aaCCGGGCGCAGACCGGAATCCATCGCCTGATTCACCTGCTGAGAATAACCATACAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCTGATCGGCAGTCAGGGTTGGTAGACGCTCACCGCTACGCATCa                                                                                                                       <  2:715824/140‑1 (MQ=255)

                cGGGCGCAGACCGGAATCCATCGCCTGATTCACCTGCTGAGAATAACCATACAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCTGATCGGCAGTCAGGGTTGGTAGACGCTCACCGCTACGCATCACTg                                                                                                                    >  1:248151/1‑140 (MQ=255)

                  ggCGCAGACCGGAATCCATCGCCTGATTCACCTGCTGAGAATAACCATACAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCTGATCGGCAGTCAGGGTTGGTAGACGCTCACCGCTACg                                                                                                                            >  2:808709/1‑130 (MQ=255)

                  ggCGCAGACCGGAATCCATCGCCTGATTCACCTGCTGAGAATAACCATACAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCTGATCGGCAGTCAGGGTTGGTAGACGCTCACCGCTACg                                                                                                                            <  1:808709/130‑1 (MQ=255)

                  ggCGCAGACCGGAATCCATCGCCTGATTCACCTGCTGAGAATAACCATACAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCTGATCGGCAGTCAGGGTTGGTAGACGCTCACCGCTACGCa                                                                                                                          >  1:103504/1‑132 (MQ=255)

                  ggCGCAGACCGGAATCCATCGCCTGATTCACCTGCTGAGAATAACCATACAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCTGATCGGCAGTCAGGGTTGGTAGACGCTCACCGCTACGCa                                                                                                                          <  2:103504/132‑1 (MQ=255)

                  ggCGCAGACCGGAATCCATCGCCTGATTCACCTGCTGAGAATAACCATACAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCTGATCGGCAGTCAGGGTTGGTAGACGCTCACCGCTACGCATCACTGTa                                                                                                                  >  1:837145/1‑140 (MQ=255)

                  ggCGCAGACCGGAATCCATCGCCTGATTCACCTGCTGAGAATAACCATACAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCTGATCGGCAGTCAGGGTTGGTAGACGCTCACCGCTACGCATCACTGTa                                                                                                                  >  1:592163/1‑140 (MQ=255)

                  ggCGCAGACCGGAATCCATCGCCTGATTCACCTGCTGAGAATAACCATACAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCTGATCGGCAGTCAGGGTTGGTAGACGCTCACCGCTACGCATCACTGTa                                                                                                                  >  1:763655/1‑140 (MQ=255)

                  ggCGCAGACCGGAATCCATCGCCTGATTCACCTGCTGAGAATAACCATACAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCTGATCGGCAGTCAGGGTTGGTAGACGCTCACCGCTACGCATCACTGTa                                                                                                                  >  1:642561/1‑140 (MQ=255)

                  ggCGCAGACCGGAATCCATCGCCTGATTCACCTGCTGAGAATAACCATACAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCTGATCGGCAGTCAGGGTTGGTAGACGCTCACCGCTACGCATCACTGTa                                                                                                                  >  1:741829/1‑140 (MQ=255)

                         aCCGGAATCCATCGCCTGATTCACCTGCTGAGAATAACCATACAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCTGATCGGCAGTCAGGGTTGGTAGACGCTCACCGCTACGCATCACTGTATTCTGCa                                                                                                           >  2:619008/1‑140 (MQ=255)

                                  cATCGCCTGATTCACCTGCTGAGAATAACCATACAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCTGATCGGCAGTCAGGGTTGGTAGACGCTCACCGCTACGCATCACTGTATTCTGCAGGAAGTCGa                                                                                                  <  2:149695/140‑1 (MQ=255)

                                     cGCCTGATTCACCTGCTGAGAATAACCATACAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCTGATCGGCAGTCAGGGTTGGTAGACGCTCACCGCTACGCATCACTGTATTCTGCAGGAAGTCGATaa                                                                                               <  2:763655/140‑1 (MQ=255)

                                     cGCCTGATTCACCTGCTGAGAATAACCATACAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCTGATCGGCAGTCAGGGTTGGTAGACGCTCACCGCTACGCATCACTGTATTCTGCAGGAAGTCGATaa                                                                                               >  1:669147/1‑140 (MQ=255)

                                            ttCACCTGCTGAGAATAACCATACAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCTGATCGGCAGTCAGGGTTGGTAGACGCTCACCGCTACGCATCACTGTATTCTGCAGGAAGTCGATAAACGCttt                                                                                        >  2:197371/1‑140 (MQ=255)

                                               aCCTGCTGAGAATAACCATACAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCTGATCGGCAGTCAGGGTTGGTAGACGCTCACCGCTACGCATCACTGTATTCTGCAGGAAGTCGATAAACGCTTTACg                                                                                     <  2:837145/140‑1 (MQ=255)

                                                ccTGTTGAGAATAACCATACAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCTGATCGGCAGTCAGGGTTGGTAGACGCTCACCGCTACGCATCACTGTATTCTGCAGGAAGTCGATAAACGCTTTACGc                                                                                    <  2:755787/140‑1 (MQ=255)

                                                      gagaATAACCATACAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCTGATCGGCAGTCAGGGTTGGTAGACGCTCACCGCTACGCATCACTGTATTCTGCAGGAAGTCGATAAACGCTTTACGCTGATCg                                                                              >  2:875638/1‑140 (MQ=255)

                                                       agaATAACCATACAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCTGATCGGCAGTCAGGGTTGGTAGACGCTCACCGCTACGCATCACTGTATTCTGCAGGAAGTCGATAAACGCTTTACGCTGATCGc                                                                             >  2:15319/1‑140 (MQ=255)

                                                       agaATAACCATACAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCTGATCGGCAGTCAGGGTTGGTAGACGCTCACCGCTACGCATCACTGTATTCTGCAGGAAGTCGATAAACGCTTTACGCTGATCGc                                                                             <  2:437116/140‑1 (MQ=255)

                                                             aCCATACAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCTGATCGGCAGTCAGGGTTGGTAGACGCTCACCGCTACGCATCACTGTATTCTGCAGGAAGTCGATAAACGCTTTACGCTGATCGCcttctt                                                                       >  1:136460/1‑140 (MQ=255)

                                                                  aCAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCCGATCGGCAGTCAGGGTTGGTAGACGCTCACCGCTACGCATCACTGTATTCTGCAGGAAGTCGATAAACGCTTTACGCTGATCGCCTTCTTTATCa                                                                  >  1:205432/1‑140 (MQ=255)

                                                                   cAGAATCGCGTAATCAGAGACAAAAGGTCCAAACTGTTTTTTCTGATCGGCAGTCAGGGTTGGTGGACGCTCACCGCTACGCATCACTGTATTCTGCCGGAAGTCGATAAACGCTTTACGCTGATCGCCTTCTTTATCaa                                                                 <  2:592163/140‑1 (MQ=255)

                                                                   cAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCTGATCGGCAGTCAGGGTTGGTAGACGCTCACCGCTACGCATCACTGTATTCTGCAGGAAGTCGATAAACGCTTTACGCTGATCGCCTTCTTTATCaa                                                                 <  1:276124/140‑1 (MQ=255)

                                                                   cAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCTGATCGGCAGTCAGGGTTGGTAGACGCTCACCGCTACGCATCACTGTATTCTGCAGGAAGTCGATAAACGCTTTACGCTGATCGCCTTCTTTATCaa                                                                 <  1:15319/140‑1 (MQ=255)

                                                                   cAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCTGATCGGCAGTCAGGGTTGGTAGACGCTCACCGCTACGCATCACTGTATTCTGCAGGAAGTCGATAAACGCTTTACGCTGATCGCCTTCTTTATCaa                                                                 <  2:537672/140‑1 (MQ=255)

                                                                   cAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCTGATCGGCAGTCAGGGTTGGTAGACGCTCACCGCTACGCATCACTGTATTCTGCAGGAAGTCGATAAACGCTTTACGCTGATCGCCTTCTTTATCaa                                                                 >  2:212022/1‑140 (MQ=255)

                                                                   cAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCTGATCGGCAGTCAGGGTTGGTAGACGCTCACCGCTACGCATCACTGTATTCTGCAGGAAGTCGATAAACGCTTTACGCTGATCGCCTTCTTTATCaa                                                                 >  2:45265/1‑140 (MQ=255)

                                                                     gAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCTGATCGGCAGTCAGGGTTGGTAGACGCTCACCGCTACGCATCACTGTATTCTGCAGGAAGTCGATAAACGCTTTACGCTGATCGCCTTCTTTATCaaaa                                                               >  2:348061/1‑140 (MQ=255)

                                                                        tCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCTGATCGGCAGTCAGGGTTGGTAGACGCTCACCGCTACGCATCACTGTATTCTGCAGGAAGTCGATAAACGCTTTACGCTGATCGCCTTCTTTATCAAAacac                                                            >  1:455974/1‑140 (MQ=255)

                                                                           cgTAATCAGAGACAAAAGGACCAAACTGTTTTTTCTGATCGGCAGTCAGGGTTGGTAGACGCTCACCGCTACGCATCACTGTATTCTGCAGGAAGTCGATAAACGCTTTACGCTGATCGCCTTCTTTATCAAAACACCCa                                                         >  1:204272/1‑140 (MQ=255)

                                                                           cgTAATCAGAGACAAAAGGACCAAACTGTTTTTTCTGATCGGCAGTCAGGGTTGGTAGACGCTCACCGCTACGCATCACTGTATTCTGCAGGAAGTCGATAAACGCTTTACGCTGATCGCCTTCTTTATCAAAACACCCa                                                         >  2:261618/1‑140 (MQ=255)

                                                                                 cAGAGACAAAAGGACCAAACTGTTTTTTCTGATCGGCAGTCAGGGTTGGTAGACGCTCACCGCTACGCATCACTGTATTCTGCAGGAAGTCGATAAACGCTTTACGCTGATCGCCTTCTTTATCAAAACACCCACTCAgg                                                   <  2:204272/140‑1 (MQ=255)

                                                                                     gaCAAAAGGACCAAACTGTTTTTTCTGATCGGCAGTCAGGGTTGGTAGACGCTCACCGCTACGCATCACTGTATTCTGCAGGAAGTCGATAAACGCTTTACGCTGATCGCCTTCTTTATCAAAACACCCACTCAGGCTaa                                               <  1:619008/140‑1 (MQ=255)

                                                                                      aCAAAAGGACCAAACTGTTTTTTCTGATCGGCAGTCAGGGTTGGTAGACGCTCACCGCTACGCATCACTGTATTCTGCAGGAAGTCGATAAACGCTTTACGCTGATCGCCTTCTTTATCAAAACACCCACTCAGGCTAAc                                              >  2:274256/1‑140 (MQ=255)

                                                                                      aCAAAAGGACCAAACTGTTTTTTCTGATCGGCAGTCAGGGTTGGTAGACGCTCACCGCTACGCATCACTGTATTCTGCAGGAAGTCGATAAACGCTTTACGCTGATCGCCTTCTTTATCAAAACACCCACTCAGGCTAAc                                              <  1:274256/140‑1 (MQ=255)

                                                                                      aCAAAAGGACCAAACTGTTTTTTCTGATCGGCAGTCAGGGTTGGTAGACGCTCACCGCTACGCATCACTGTATTCTGCAGGAAGTCGATAAACGCTTTACGCTGATCGCCTTCTTTATCAAAACACCCACTCAGGCTAAc                                              <  2:669147/140‑1 (MQ=255)

                                                                                       cAAAAGGACCAAACTGTTTTTTCTGATCGGCAGTCAGGGTTGGTAGACGCTCACCGCTACGCATCACTGTATTCTGCAGGAAGTCGATAAACGCTTTACGCTGATCGCCTTCTTTATCAAAACCCCCCCTCAGGCTAACt                                             <  2:248151/140‑1 (MQ=255)

                                                                                        aaaaGGACCAAACTGTTTTTTCTGATCGGCAGTCAGGGTTGGTAGACGCTCACCGCTACGCATCACTGTATTCTGCAGGAAGTCGATAAACGCTTTACGCTGATCGCCTTCTTTATCAAAACACCCACTCAGGCTAACTa                                            <  1:197371/140‑1 (MQ=255)

                                                                                          aaGGACCAAACTGTTTTTTCTGATCGGCAGTCAGGGTTGGTAGACGCTCACCGCTACGCATCACTGTATTCTGCAGGAAGTCGATAAACGCTTTACGCTGATCGCCTTCTTTATCAAAACACCCACTCAGGCTAACTAcc                                          >  2:595703/1‑140 (MQ=255)

                                                                                              aCCAAACTGTTTTTTCTGATCGGCAGTCAGGGTTGGTAGACGCTCACCGCTACGCATCACTGTATTCTGCAGGAAGTCGATAAACGCTTTACGCTGATCGCCTTCTTTATCAAAACACCCACTCAGGCTAACTACCATta                                      >  2:613651/1‑140 (MQ=255)

                                                                                                     tGTTTTTTCTGATCGGCAGTCAGGGTTGGTAGACGCTCACCGCTACGCATCACTGTATTCTGCAGGAAGTCGATAAACGCTTTACGCTGATCGCCTTCTTTATCAAAACACCCACTCAGGCTAACTACCATTAATAAcgc                               >  1:736426/1‑140 (MQ=255)

                                                                                                        tttttCTGATCGGCAGTCAGGGTTGGTAGACGCTCACCGCTACGCATCACTGTATTCTGCAGGAAGTCGATAAACGCTTTACGCTGATCGCCTTCTTTATCAAAACACCCACTCAGGCTAACTACCATTAATAAcgccgc                            >  1:441976/1‑140 (MQ=255)

                                                                                                           ttCTGATCGGCAGTCAGGGTTGGTAGACGCTCACCGCTACGCATCACTGTATTCTGCAGGAAGTCGATAAACGCTTTACGCTGATCGCCTTCTTTATCAAAACACCCACTCAGGCTAACTACCATTAATAACGCCGCAag                         <  2:619912/140‑1 (MQ=255)

                                                                                                               gATCGGCAGTCAGGGTTGGTAGACGCTCACCGCTACGCATCACTGTATTCTGCAGGAAGTCGATAAACGCTTTACGCTGATCGCCTTCTTTATCAAAACACCCACTCAGGCTAACTACCATTAATAACGCCGCAAGAGgc                     <  1:595703/140‑1 (MQ=255)

                                                                                                                 tCGGCAGTCAGGGTTGGTAGACGCTCACCGCTACGCATCACTGTATTCTGCAGGAAGTCGATAAACGCTTTACGCTGATCGCCTTCTTTATCAAAACACCCACTCAGGCTAACTACCATTAATAACGCCGCAAGAGgcgc                   <  1:875638/140‑1 (MQ=255)

                                                                                                                     cAGTCAGGGTTGGTAGACGCTCACCGCTACGCATCACTGTATTCTGCAGGAAGTCGATAAACGCTTTACGCTGATCGCCTTCTTTATCAAAACACCCACTCAGGCTAACTACCATTAATAACGCCGCAAGAGGCGCAAAc               >  2:208574/1‑140 (MQ=255)

                                                                                                                                 gTAGACGCTCACCGCTACGCATCACTGTATTCTGCAGGAAGTCGATAAACGCTTTACGCTGATCGCCTTCTTTATCAAAACACCCACTCAGGCTAACTACCATTAATAACGCCGCAAGAGGCGCAAACCAGCGAGAGCAg   >  2:53347/1‑140 (MQ=255)

                                                                                                                                  tAGACGCTCACCGCTACGCATCACTGTATTCTGCAGGAAGTCGATAAACGCTTTACGCTGATCGCCTTCTTTATCAAAACACCCACTCAGGCTAACTACCATTAATAACGCCGCAAGAGGCGCAAACCAGCGAGAGCAgg  >  2:671520/1‑140 (MQ=255)

                                                                                                                                  tAGACGCTCACCGCTACGCATCACTGTATTCTGCAGGAAGTCGATAAACGCTTTACGCTGATCGCCTTCTTTATCAAAACACCCACTCAGGCTAACTACCATTAATAACGCCGCAAGAGGCGCAAACCAGCGAGAGCAgg  >  2:289683/1‑140 (MQ=255)

TAACGCTGTCTACAACCGGGCGCAGACCGGAATCCATCGCCTGATTCACCTGCTGAGAATAACCATACAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCTGATCGGCAGTCAGGGTTGGTAGACGTTCACCGCTACGCATCACTGTATTCTGCAGGAAGTCGATAAACGCTTTACGCTGATCGCCTTCTTTATCAAAACACCCACTCAGGCTAACTACCATTAATAACGCCGCAAGAGGCGCAAACCAGCGAGAGCAGG  >  NC_000913/3718759‑3719028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A