Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	155	60.3	2039550	99.3%	2025273	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,748,607	G→A	D11N (GAC→AAC)	sgbH →	3‑keto‑L‑gulonate‑6‑phosphate decarboxylase SgbH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,748,607	0	G	A	100.0%	209.5 / NA	59	D11N (GAC→AAC)	sgbH	3‑keto‑L‑gulonate‑6‑phosphate decarboxylase SgbH
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (35/24); total (35/24)

CAGATATGACCGCCCATCAGCTTTACCAAAAAAAATATCAACGTTATCAGCATCTCATTGCCGCACTTCAGGGCTTTCACGCCCGCATTAAGGAGCACACATTATGAGCCGACCACTTCTGCAACTGGCCCTCGACCACTCATCACTTGAAGCCGCGCAGCGCGACGTGACGCTGTTAAAAGACAGCGTCGATATCGTCGAAGCGGGCACCATTCTCTGTTTAAACGAAGGGCTTGGCGCGGTGAAAGCCTTGCGCGAACAGTGCC  >  NC_000913/3748474‑3748739

cAGATATGACCGCCCATCAGCTTTACCAAAAAAAATATCAACGTTATCTGCATCTCATTGCCGCACTTCAGGGCTTTCACGCCCGCATTAAGGAGCACACATTATGAGCCGACCACTTCTGCAACTGGCCCTCAACCACt                                                                                                                                >  2:872565/1‑140 (MQ=255)

cAGATATGACCGCCCATCAGCTTTACCAAAAAAAATATCAACGTTATCAGCATCTCATTGCCGCACTTCAGGGCTTTCACGCCCGCATTAAGGAGCACACATTATGAGCCGACCACTTCTGCAACTGGCCCTCAACCACt                                                                                                                                <  1:944043/140‑1 (MQ=255)

cAGATATGACCGCCCATCAGCTTTACCAAAAAAAATATCAACGTTATCAGCATCTCATTGCCGCACTTCAGGGCTTTCACGCCCGCATTAAGGAGCACACATTATGAGCCGACCACTTCTGCAACTGGCCCTCAACCACt                                                                                                                                <  1:892946/140‑1 (MQ=255)

  gATATGACCGCCCATCAGCTTTACCAAAAAAAATATCAACGTTATCAGCATCTCATTGCCGCACTTCAGGGCTTTCACGCCCGCATTAAGGAGCACACATTATGAGCCGACCACTTCTGCAACTGGCCCTCAACCACtca                                                                                                                              <  2:451757/140‑1 (MQ=255)

  gATATGACCGCCCATCAGCTTTACCAAAAAAAATATCAACGTTATCAGCATCTCATTGCCGCACTTCAGGGCTTTCACGCCCGCATTAAGGAGCACACATTATGAGCCGACCACTTCT‑CAACTGGCCCTCAACCACtcat                                                                                                                             >  1:461687/1‑140 (MQ=255)

   atatGACCGCCCATCAGCTTTACCAAAAAAAATATCAACGTTATCAGCATCTCATTGCCGCACTTCAGGGCTTTCACGCCCGCATTAAGGAGCACACATTATGAGCCGACCACTTCTGCAACTGGCCCTCAACCACtcat                                                                                                                             <  2:938054/140‑1 (MQ=255)

        aCCGCCCATCAGCTTTACCAAAAAAAATATCAACGTTATCAGCATCTCATTGCCGCACTTCAGGGCTTTCACGCCCGCATTAAGGAGCACACATTATGAGCCGACCACTTCTGCAACTGGCCCTCAACCACTCATCACtt                                                                                                                        >  1:467827/1‑140 (MQ=255)

         ccGCCCATCAGCTTTACCAAAAAAAATATCAACGTTATCAGCATCTCATTGCCGCACTTCAGGGCTTTCACGCCCGCATTAAGGAGCACACATTATGAGCCGACCACTTCTGCAACTGGCCCTCAACCACTCATCACTTg                                                                                                                       >  2:578245/1‑140 (MQ=255)

         ccGCCCATCAGCTTTACCAAAAAAAATATCAACGTTATCAGCATCTCATTGCCGCACTTCAGGGCTTTCACGCCCGCATTAAGGAGCACACATTATGAGCCGACCACTTCTGCAACTGGCCCTCAACCACTCATCACTTg                                                                                                                       >  2:1002460/1‑140 (MQ=255)

            cccATCAGCTTTACCAAAAAAAATATCAACGTTATCAGCATCTCATTGCCGCACTTCAGGGCTTTCACGCCCGCATTAAGGAGCACACATTATGAGCCGACCACTTCTGCAACTGGCCCTCAACCACTCATCACTTGAAg                                                                                                                    >  1:918059/1‑140 (MQ=255)

            cccATCAGCTTTACCAAAAAAAATATCAACGTTATCAGCATCTCATTGCCGCACTTCAGGGCTTTCACGCCCGCATTAAGGAGCACACATTATGAGCCGACCACTTCTGCAACTGGCCCTCAACCACTCATCACTTGAAg                                                                                                                    <  1:578245/140‑1 (MQ=255)

             ccATCAGCTTTACCAAAAAAAATATCAACGTTATCAGCATCTCATTGCCGCACTTCAGGGCTTTCACGCCCGCATTAAGGAGCACACATTATGAGCCGACCACTTCTGCAACTGGCCCTCAACCACTCATCACTTGAAGc                                                                                                                   >  1:55861/1‑140 (MQ=255)

             ccATCAGCTTTACCAAAAAAAATATCAACGTTATCAGCATCTCATTGCCGCACTTCAGGGCTTTCACGCCCGCATTAAGGAGCACACATTATGAGCCGACCACTTCTGCAACTGGCCCTCAACCACTCATCACTTGAAGc                                                                                                                   >  2:669614/1‑140 (MQ=255)

                    cTTTACCAAAAAAAATATCAACGTTATCAGCATCTCATTGCCGCACTTCAGGGCTTTCACGCCCGCATTAAGGAGCACACATTATGAGCCGACCACTTCTGCAACTGGCCCTCAACCACTCATCACTTGAAGCCGCGCAg                                                                                                            >  2:54877/1‑140 (MQ=255)

                       tACCAAAAAAAATATCAACGTTATCAGCATCTCATTGCCGCACTTCAGGGCTTTCACGCCCGCATTAAGGAGCACACATTATGAGCCGACCACTTCTGCAACTGGCCCTCAACCACTCATCACTTGAAGCCGCGCAgcgc                                                                                                         >  1:392461/1‑140 (MQ=255)

                             aaaaaaTATCAACGTTATCAGCATCTCATTGCCGCACTTCAGGGCTTTCACGCCCGCATTAAGGAGCACACATTATGAGCCGACCACTTCTGCAACTGGCCCTCAACCACTCATCACTTGAAGCCGCGCAGCGCGACGTg                                                                                                   >  1:667613/1‑140 (MQ=255)

                              aaaaaTATCAACGTTATCAGCATCTCATTGCCGCACTTCAGGGCTTTCACGCCCGCATTAAGGAGCACACATTATGAGCCGACCACTTCTGCAACTGGCCCTCAACCACTCATCACTTGAAGCCGCGCAGCGCGACGTGa                                                                                                  >  2:78957/1‑140 (MQ=255)

                               aaaaTATCAACGTTATCAGCATCTCATTGCCGCACTTCAGGGCTTTCACGCCCGCATTAAGGAGCACACATTATGAGCCGACCACTTCTGCAACTGGCCCTCAACCACTCATCACTTGAAGCCGCGCAGCGCGACGTGAc                                                                                                 <  1:78957/140‑1 (MQ=255)

                                 aaTATCAACGTTATCAGCATCTCATTGCCGCACTTCAGGGCTTTCACGCCCGCATTAAGGAGCACACATTATGAGCCGACCACTTCTGCAACTGGCCCTCAACCACTCATCACTTGAAGCCGCGCAGCGCGACGTGACGc                                                                                               <  1:133954/140‑1 (MQ=255)

                                 aaTATCAACGTTATCAGCATCTCATTGCCGCACTTCAGGGCTTTCACGCCCGCATTAAGGAGCACACATTATGAGCCGACCACTTCTGCAACTGGCCCTCAACCACTCATCACTTGAAGCCGCGCAGCGCGACGTGACGc                                                                                               <  1:458257/140‑1 (MQ=255)

                                 aaTATCAACGTTATCAGCATCTCATTGCCGCACTTCAGGGCTTTCACGCCCGCATTAAGGAGCACACATTATGAGCCGACCACTTCTGCAACTGGCCCTCAACCACTCATCACTTGAAGCCGCGCAGCGCGACGTGACGc                                                                                               <  2:924024/140‑1 (MQ=255)

                                 aaTATCAACGTTATCAGCATCTCATTGCCGCACTTCAGGGCTTTCACGCCCGCATTAAGGAGCACACATTATGAGCCGACCACTTCTGCAACTGGCCCTCAACCACTCATCACTTGAAGCCGCGCAGCGCGACGTGACGc                                                                                               <  2:55861/140‑1 (MQ=255)

                                 aaTATCAACGTTATCAGCATCTCATTGCCGCACTTCAGGGCTTTCACGCCCGCATTAAGGAGCACACATTATGAGCCGACCACTTCTGCAACTGGCCCTCAACCACTCATCACTTGAAGCCGCGCAGCGCGACGTGACGc                                                                                               <  2:467827/140‑1 (MQ=255)

                                 aaTATCAACGTTATCAGCATCTCATTGCCGCACTTCAGGGCTTTCACGCCCGCATTAAGGAGCACACATTATGAGCCGACCACTTCTGCAACTGGCCCTCAACCACTCATCACTTGAAGCCGCGCAGCGCGACGTGACGc                                                                                               <  1:54877/140‑1 (MQ=255)

                                         cGTTATCAGCATCTCATTGCCGCACTTCAGGGCTTTCACGCCCGCATTAAGGAGCACACATTATGAGCCGACCACTTCTGCAACTGGCCCTCAACCACTCATCACTTGAAGCCGCGCAGCGCGACGTGACGCTGTTaaaa                                                                                       <  1:1002460/140‑1 (MQ=255)

                                           ttATCAGCATCTCATTGCCGCACTTCAGGGCTTTCACGCCCGCATTAAGGAGCACACATTATGAGCCGACCACTTCTGCAACTGGCCCTCAACCACTCATCACTTGAAGCCGCGCAGCGCGACGTGACGCTGTTAAAAGa                                                                                     <  1:308493/140‑1 (MQ=255)

                                                    tctcATTGCCGCACTTCAGGGCTTTCACGCCCGCATTAAGGAGCACACATTATGAGCCGACCACTTCTGCAACTGGCCCTCAACCACTCATCACTTGAAGCCGCGCAGCGCGACGTGACGCTGTTAAAAGACAGCGTCGa                                                                            >  1:671932/1‑140 (MQ=255)

                                                     ctcATTGCCGCACTTCAGGGCTTTCACGCCCGCATTAAGGAGCACACATTATGAGCCGACCACTTCTGCAACTGGCCCTCAACCACTCATCACTTGAAGCCGCGCAGCGCGACGTGACGCTGTTAAAAGACAGCGTCGat                                                                           >  2:738563/1‑140 (MQ=255)

                                                           gCCGCACTTCAGGGCTTTCACGCCCGCATTAAGGAGCACACATTATGAGCCGACCACTTCTGCAACTGGCCCTCAACCACTCATCACTTGAAGCCGCGCAGCGCGACGTGACGCTGTTAAAAGACAGCGTCGATAtcgtc                                                                     <  2:918059/140‑1 (MQ=255)

                                                            ccGCACTTCAGGGCTTTCACGCCCGCATTAAGGAGCACACATTATGAGCCGACCACTTCTGCAACTGGCCCTCAACCACTCATCACTTGAAGCCGCGCAGCGCGACGTGACGCTGTTAAAAGACAGCGTCGATAtcgtcg                                                                    >  1:575678/1‑140 (MQ=255)

                                                                  ttCAGGGCTTTCACGCCCGCATTAAGGAGCACACATTATGAGCCGACCACTTCTGCAACTGGCCCTCAACCACTCATCACTTGAAGCCGCGCAGCGCGACGTGACGCTGTTAAAAGACAGCGTCGATATCGTCGAAGCgg                                                              >  2:406975/1‑140 (MQ=255)

                                                                            tCACGCCCGCATTAAGGAGCACACATTATGAGCCGACCACTTCTGCAACTGGCCCTCAACCACTCATCACTTGAAGCCGCGCAGCGCGACGTGACGCTGTTAAAAGACAGCGTCGATATCGTCGAAGCGGGCACCATtct                                                    >  2:549769/1‑140 (MQ=255)

                                                                             cACGCCCGCATTAAGGAGCACACATTATGAGCCGACCACTTCTGCAACTGGCCCTCAACCACTCATCACTTGAAGCCGCGCAGCGCGACGTGACGCTGTTAAAAGACAGCGTCGATATCGTCGAAGCGGGCACCATtctc                                                   <  2:667613/140‑1 (MQ=255)

                                                                                     cATTAAGGAGCACACATTATGAGCCGACCACTTCTGCAACTGGCCCTCAACCACTCATCACTTGAAGCCGCGCAGCGCGACGTGACGCTGTTAAAAGACAGCGTCGATATCGTCGAAGCGGGCACCATTCTCTGTTTaaa                                           >  1:346108/1‑140 (MQ=255)

                                                                                        tAAGGAGCACACATTATGAGCCGACCACTTCTGCAACTGGCCCTCAACCACTCATCACTTGAAGCCGCGCAGCGCGACGTGACGCTGTTAAAAGACAGCGTCGATATCGTCGAAGCGGGCACCATTCTCTGTTTAAACGa                                        <  2:346108/140‑1 (MQ=255)

                                                                                                   caTTATGAGCCGACCACTTCTGCAACTGGCCCTCAACCACTCATCACTTGAAGCCGCGCAGCGCGACGTGACGCTGTTAAAAGACAGCGTCGATATCGTCGAAGCGGGCACCATTCTCTGTTTAAACGAAGGGCTTGgcg                             <  1:872565/140‑1 (MQ=255)

                                                                                                   caTTATGAGCCGACCACTTCTGCAACTGGCCCTCAACCACTCATCACTTGAAGCCGCGCAGCGCGACGTGACGCTGTTAAAAGACAGCGTCGATATCGTCGAAGCGGGCACCATTCTCTGTTTAAACGAAGGGCTTGgcg                             <  1:717803/140‑1 (MQ=255)

                                                                                                      tATGAGCCGACCACTTCTGCAACTGGCCCTCAACCACTCATCACTTGAAGCCGCGCAGCGCGACGTGACGCTGTTAAAAGACAGCGTCGATATCGTCGAAGCGGGCACCATTCTCTGTTTAAACGAAGGGCTTGGCGCgg                          >  2:830413/1‑140 (MQ=255)

                                                                                                       aTGAGCCGACCACTTCTGCAACTGGCCCTCAACCACTCATCACTTGAAGCCGCGCAGCGCGACGTGACGCTGTTAAAAGACAGCGTCGATATCGTCGAAGCGGGCACCATTCTCTGTTTAAACGAAGGGCTTGGCGCGGt                         >  2:325086/1‑140 (MQ=255)

                                                                                                         gAGCCGACCACTTCTGCAACTGGCCCTCAACCACTCATCACTTGAAGCCGCGCAGCGCGACGTGACGCTGTTAAAAGACAGCGTCGATATCGTCGAAGCGGGCACCATTCTCTGTTTAAACGAAGGGCTTGGCGCGGTGa                       >  2:1016393/1‑140 (MQ=255)

                                                                                                            ccGACCACTTCTGCAACTGGCCCTCAACCACTCATCACTTGAAGCCGCGCAGCGCGACGTGACGCTGTTAAAAGACAGCGTCGATATCGTCGAAGCGGGCACCATTCTCTGTTTAAACGAAGGGCTTGGCGCGGTGAAAg                    >  2:35386/1‑140 (MQ=255)

                                                                                                            ccGACCACTTCTGCAACTGGCCCTCAACCACTCATCACTTGAAGCCGCGCAGCGCGACGTGACGCTGTTAAAAGACAGCGTCGATATCGTCGAAGCGGGCACCATTCTCTGTTTAAACGAAGGGCTTGGCGCGGTGAAAg                    >  1:656409/1‑140 (MQ=255)

                                                                                                            ccGACCACTTCTGCAACTGGCCCTCAACCACTCATCACTTGAAGCCGCGCAGCGCGACGTGACGCTGTTAAAAGACAGCGTCGATATCGTCGAAGCGGGCACCATTCTCTGTTTAAACGAAGGGCTTGGCGCGGTGAAAg                    >  1:684411/1‑140 (MQ=255)

                                                                                                              gACCACTTCTGCAACTGGCCCTCAACCACTCATCACTTGAAGCCGCGCAGCGCGACGTGACGCTGTTAAAAGACAGCGTCGATATCGTCGAAGCGGGCACCATTCTCTGTTTAAACGAAGGGCTTGgcg                             >  1:602288/1‑129 (MQ=255)

                                                                                                              gACCACTTCTGCAACTGGCCCTCAACCACTCATCACTTGAAGCCGCGCAGCGCGACGTGACGCTGTTAAAAGACAGCGTCGATATCGTCGAAGCGGGCACCATTCTCTGTTTAAACGAAGGGCTTGgcg                             <  2:602288/129‑1 (MQ=255)

                                                                                                              gACCACTTCTGCAACTGGCCCTCAACCACTCATCACTTGAAGCCGCGCAGCGCGACGTGACGCTGTTAAAAGACAGCGTCGATATCGTCGAAGCGGGCACCATTCTCTGTTTAAACGAAGGGCTTGgc                              >  2:578332/1‑128 (MQ=255)

                                                                                                              gACCACTTCTGCAACTGGCCCTCAACCACTCATCACTTGAAGCCGCGCAGCGCGACGTGACGCTGTTAAAAGACAGCGTCGATATCGTCGAAGCGGGCACCATTCTCTGTTTAAACGAAGGGCTTGgc                              <  1:578332/128‑1 (MQ=255)

                                                                                                              gACCACTTCTGCAACTGGCCCTCAACCACTCATCACTTGAAGCCGCGCAGCGCGACGTGACGCTGTTAAAAGACAGCGTCGATATCGTCGAAGCGGGCACCATTCTCTGTTTAAACGAAGGGCTTGGCGCGGTGAAAGcc                  >  2:359687/1‑140 (MQ=255)

                                                                                                              gACCACTTCTGCAACTGGCCCTCAACCACTCATCACTTGAAGCCGCGCAGCGCGACGTGACGCTGTTAAAAGACAGCGTCGATATCGTCGAAGCGGGCACCATTCTCTGTTTAAACGAAGGGCTTGGCGCGGTGAAAGcc                  >  1:68910/1‑140 (MQ=255)

                                                                                                                ccACTTCTGCAACTGGCCCTCAACCACTCATCACTTGAAGCCGCGCAGCGCGACGTGACGCTGTTAAAAGACAGCGTCGATATCGTCGAAGCGGGCACCATTCTCTGTTTAAACGAAGGGCTTGgcg                             >  2:364568/1‑127 (MQ=255)

                                                                                                                ccACTTCTGCAACTGGCCCTCAACCACTCATCACTTGAAGCCGCGCAGCGCGACGTGACGCTGTTAAAAGACAGCGTCGATATCGTCGAAGCGGGCACCATTCTCTGTTTAAACGAAGGGCTTGgcg                             <  1:364568/127‑1 (MQ=255)

                                                                                                                      cTGCAACTGGCCCTCAACCACTCATCACTTGAAGCCGCGCAGCGCGACGTGACGCTGTTAAAAGACAGCGTCGATATCGTCGAAGCGGGCACCATTCTCTGTTTAAACGAAGGGCTTGGCGCGGTGAAAGCCTTGCGCGa          >  1:689180/1‑140 (MQ=255)

                                                                                                                        gCAACTGGCCCTCAACCACTCATCACTTGAAGCCGCGCAGCGCGACGTGACGCTGTTAAAAGACAGCGTCGATATCGTCGAAGCGGGCACCATTCTCTGTTTAAACGAAGGGCTTGGCGCGGTGAAAGCCTTGCGCGAAc        <  1:35386/140‑1 (MQ=255)

                                                                                                                        gCAACTGGCCCTCAACCACTCATCACTTGAAGCCGCGCAGCGCGACGTGACGCTGTTAAAAGACAGCGTCGATATCGTCGAAGCGGGCACCATTCTCTGTTTAAACGAAGGGCTTGGCGCGGTGAAAGCCTTGCGCGAAc        <  1:359687/140‑1 (MQ=255)

                                                                                                                              ggCCCTCAACCACTCATCACTTGAAGCCGCGCAGCGCGACGTGACGCTGTTAAAAGACAGCGTCGATATCGTCGAAGCGGGCACCATTCTCTGTTTAAACGAAGGGCTTGGCGCGGTGAAAGCCTTGCGCGAACAGTGcc  >  1:271209/1‑140 (MQ=255)

                                                                                                                              ggCCCTCAACCACTCATCACTTGAAGCCGCGCAGCGCGACGTGACGCTGTTAAAAGACAGCGTCGATATCGTCGAAGCGGGCACCATTCTCTGTTTAAACGAAGGGCTTGGCGCGGTGAAAGCCTTGCGCGAACAGTGcc  >  2:861699/1‑140 (MQ=255)

                                                                                                                              ggCCCTCAACCACTCATCACTTGAAGCCGCGCAGCGCGACGTGACGCTGTTAAAAGACAGCGTCGATATCGTCGAAGCGGGCACCATTCTCTGTTTAAACGAAGGGCTTGGCGCGGTGAAAGCCTTGCGCGAACAGTGcc  >  2:864720/1‑140 (MQ=255)

                                                                                                                              ggCCCTCAACCACTCATCACTTGAAGCCGCGCAGCGCGACGTGACGCTGTTAAAAGACAGCGTCGATATCGTCGAAGCGGGCACCATTCTCTGTTTAAACGAAGGGCTTGGCGCGGTGAAAGCCTTGCGCGAACAGTGcc  >  1:20592/1‑140 (MQ=255)

                                                                                                                              ggCCCTCAACCACTCATCACTTGAAGCCGCGCAGCGCGACGTGACGCTGTTAAAAGACAGCGTCGATATCGTCGAAGCGGGCACCATTCTCTGTTTAAACGAAGGGCTTGGCGCGGTGAAAGCCTTGCGCGAACAGTGcc  >  2:246754/1‑140 (MQ=255)

CAGATATGACCGCCCATCAGCTTTACCAAAAAAAATATCAACGTTATCAGCATCTCATTGCCGCACTTCAGGGCTTTCACGCCCGCATTAAGGAGCACACATTATGAGCCGACCACTTCTGCAACTGGCCCTCGACCACTCATCACTTGAAGCCGCGCAGCGCGACGTGACGCTGTTAAAAGACAGCGTCGATATCGTCGAAGCGGGCACCATTCTCTGTTTAAACGAAGGGCTTGGCGCGGTGAAAGCCTTGCGCGAACAGTGCC  >  NC_000913/3748474‑3748739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A