Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	155	60.3	2039550	99.3%	2025273	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,841,006	T→C	N245S (AAC→AGC)	nepI ←	purine ribonucleoside exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,841,006	0	T	C	100.0%	179.5 / NA	51	N245S (AAC→AGC)	nepI	purine ribonucleoside exporter
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (28/23); total (28/23)

TATTAACGGCGCGCCTGCTAAGGCCAGTTTTACCGAACGTTTAAGAATGAACGACGAAAGCGACGTACCAATAAAGCTGGCGATACCAAAACTCAACAGCACCAGCGTTAAGCCATCCACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGATGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCC  >  NC_000913/3840870‑3841142

tATTAACGGCGCGCCTGCTAAGGCCAGTTTTACCGAACGTTTAAGAATGAACGACGAAAGCGACGTACCAATAAAGCTGGCGATACCAAAACTCAACAGCACCAGCGTTAAGCCATCCACGCCGAATCCCGCCAGGCTCa                                                                                                                                       >  2:661171/1‑140 (MQ=255)

tATTAACGGCGCGCCTGCTAAGGCCAGTTTTACCGAACGTTTAAGAATGAACGACGAAAGCGACGTACCAATAAAGCTGGCGATACCAAAACTCAACAGCACCAGCGTTAAGCCATCCACGCCGAATCCCGCCAGGCTCa                                                                                                                                       >  1:336057/1‑140 (MQ=255)

tATTAACGGCGCGCCTGCTAAGGCCAGTTTTACCGAACGTTTAAGAATGAACGACGAAAGCGACGTACCAATAAAGCTGGCGATACCAAAACTCAACAGCACCAGCGTTAAGCCATCCACGCCGAATCCCGCCAGGCTCa                                                                                                                                       >  2:11301/1‑140 (MQ=255)

  ttAACGGCGCGCCTGCTAAGGCCAGTTTTACCGAACGTTTAAGAATGAACGACGAAAGCGACGTACCAATAAAGCTGGCGATACCAAAACTCAACAGCACCAGCGTTAAGCCATCCACGCCGAATCCCGCCAGGCTCata                                                                                                                                     >  2:51248/1‑140 (MQ=255)

          gcgcCTGCTAAGGCCAGTTTTACCGAACGTTTAAGAATGAAGGACGAAAGCGACGTACCAATAAAGCTGGCGATACCAAAACTCAACAGCACCAGCGTTAAGCCATCCACGCCGAATCCCGCCAGGCTCATATACACCgg                                                                                                                             >  1:660907/1‑140 (MQ=255)

          gcgcCTGCTAAGGCCAGTTTTACCGAACGTTTAAGAATGAACGACGAAAGCGACGTACCAATAAAGCTGGCGATACCAAAACTCAACAGCACCAGCGTTAAGCCATCCACGCCGAATCCCGCCAGGCTCATATACACCgg                                                                                                                             >  2:240834/1‑140 (MQ=255)

          gcgcCTGCTAAGGCCAGTTTTACCGAACGTTTAAGAATGAACGACGAAAGCGACGTACCAATAAAGCTGGCGATACCAAAACTCAACAGCACCAGCGTTAAGCCATCCACGCCGAATCCCGCCAGGCTCATATACACCgg                                                                                                                             >  1:31293/1‑140 (MQ=255)

          gcgcCTGCTAAGGCCAGTTTTACCGAACGTTTAAGAATGAACGACGAAAGCGACGTACCAATAAAGCTGGCGATACCAAAACTCAACAGCACCAGCGTTAAGCCATCCACGCCGAATCCCGCCAGGCTCATATACACCgg                                                                                                                             >  1:851704/1‑140 (MQ=255)

          gcgcCTGCTAAGGCCAGTTTTACCGAACGTTTAAGAATGAACGACGAAAGCGACGTACCAATAAAGCTGGCGATACCAAAACTCAACAGCACCAGCGTTAAGCCATCCACGCCGAATCCCGCCAGGCTCATATACACCgg                                                                                                                             >  2:143329/1‑140 (MQ=255)

           cgcCTGCTAAGGCCAGTTTTACCGAACGTTTAAGAATGAACGACGAAAGCGACGTACCAATAAAGCTGGCGATACCAAAACTCAACAGCACCAGCGTTAAGCCATCCACGCCGAATCCCGCCAGGCTCATATACACCggg                                                                                                                            <  1:79821/140‑1 (MQ=255)

                           ttttACCGAACGTTTAAGAATGAACGACGAAAGCGACGTACCAATAAAGCTGGCGATACCAAAACTCAACAGCACCAGCGTTAAGCCATCCACGCCGAATCCCGCCAGGCTCATATACACCGGGCGAATATACGTgaaga                                                                                                            >  1:75119/1‑140 (MQ=255)

                           ttttACCGAACGTTTAAGAATGAACGACGAAAGCGACGTACCAATAAAGCTGGCGATACCAAAACTCAACAGCACCAGCGTTAAGCCATCCACGCCGAATCCCGCCAGGCTCATATACACCGGGCGAATATACGTgaaga                                                                                                            >  1:762984/1‑140 (MQ=255)

                             ttACCGAACGTTTAAGAATGAACGACGAAAGCGACGTACCAATAAAGCTGGCGATACCAAAACTCAACAGCACCAGCGTTAAGCCATCCACGCCGAATCCCGCCAGGCTCATATACACCGGGCGAATATACGTGAAGaaa                                                                                                          <  1:51248/140‑1 (MQ=255)

                                     cGTTTAAGAATGAACGACGAAAGCGACGTACCAATAAAGCTGGCGATACCAAAACTCAACAGCACCAGCGTTAAGCCATCCACGCCGAATCCCGCCAGGCTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTg                                                                                                  >  2:181314/1‑140 (MQ=255)

                                          aaGAATGAACGACGAAAGCGACGTACCAATAAAGCTGGCGATACCAAAACTCAACAGCACCAGCGTTAAGCCATCCACGCCGAATCCCGCCAGGCTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCgg                                                                                             <  2:877041/140‑1 (MQ=255)

                                            gAATGAACGACGAAAGCGACGTACCAATAAAGCTGGCGATACCAAAACTCAACAGCACCAGCGTTAAGCCATCCACGCCGAATCCCGCCAGGCTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCg                                                                                           <  2:446104/140‑1 (MQ=255)

                                             aaTGAACGACGAAAGCGACGTACCAATAAAGCTGGCGATACCAAAACTCAACAGCACCAGCGTTAAGCCATCCACGCCGAATCCCGCCAGGCTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGa                                                                                          <  2:31293/140‑1 (MQ=255)

                                             aaTGAACGACGAAAGCGACGTACCAATAAAGCTGGCGATACCAAAACTCAACAGCACCAGCGTTAAGCCATCCACGCCGAATCCCGCCAGGCTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGa                                                                                          <  2:762984/140‑1 (MQ=255)

                                                gAACGACGAAAGCGACGTACCAATAAAGCTGGCGATACCAAAACTCAACAGCACCAGCGTTAAGCCATCCACGCCGAATCCCGCCAGGCTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAg                                                                                       >  2:574767/1‑140 (MQ=255)

                                                 aaCGACGAAAGCGACGTACCAATAAAGCTGGCGATACCAAAACTCAACAGCACCAGCGTTAAGCCATCCACGCCGAATCCCGCCAGGCTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGa                                                                                      >  2:560168/1‑140 (MQ=255)

                                                  acgacgAAAGCGACGTACCAATAAAGCTGGCGATACCAAAACTCAACAGCACCAGCGTTAAGCCATCCACGCCGAATCCCGCCAGGCTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGAc                                                                                     >  2:436966/1‑140 (MQ=255)

                                                   cgacgaAAGCGACGTACCAATAAAGCTGGCGATACCAAAACTCAACAGCACCAGCGTTAAGCCATCCACGCCGAATCCCGCCAGGCTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACa                                                                                    <  1:560168/140‑1 (MQ=255)

                                                     acgaAAGCGACGTACCAATAAAGCTGGCGATACCAAAACTCAACAGCACCAGCGTTAAGCCATCCACGCCGAATCCCGCCAGGCTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATg                                                                                  <  1:574767/140‑1 (MQ=255)

                                                      cgaAAGCGACGTACCAATAAAGCTGGCGATACCAAAACTCAACAGCACCAGCGTTAAGCCATCCACGCCGAATCCCGCCAGGCTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGa                                                                                 >  2:530656/1‑140 (MQ=255)

                                                      cgaAAGCGACGTACCAATAAAGCTGGCGATACCAAAACTCAACAGCACCAGCGTTAAGCCATCCACGCCGAATCCCGCCAGGCTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGa                                                                                 >  2:334687/1‑140 (MQ=255)

                                                        aaaGCGACGTACCAATAAAGCTGGCGATACCAAAACTCAACAGCACCAGCGTTAAGCCATCCACGCCGAATCCCGCCAGGCTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGcc                                                                                                <  1:846887/123‑1 (MQ=255)

                                                        aaaGCGACGTACCAATAAAGCTGGCGATACCAAAACTCAACAGCACCAGCGTTAAGCCATCCACGCCGAATCCCGCCAGGCTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGcc                                                                                                >  2:846887/1‑123 (MQ=255)

                                                          aGCGACGTACCAATAAAGCTGGCGATACCAAAACTCAACAGCACCAGCGTTAAGCCATCCACGCCGAATCCCGCCAGGCTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGAt                                                                             <  1:530656/140‑1 (MQ=255)

                                                          aGCGACGTACCAATAAAGCTGGCGATACCAAAACTCAACAGCACCAGCGTTAAGCCATCCACGCCGAATCCCGCCAGGCTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGAt                                                                             <  2:851704/140‑1 (MQ=255)

                                                              aCGTACCAATAAAGCTGGCGATACCAAAACTCAACAGCACCAGCGTTAAGCCATCCACGCCGAATCCCGCCAGGCTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGATGGCg                                                                         <  2:58572/140‑1 (MQ=255)

                                                                  aCCAATAAAGCTGGCGATACCAAAACTCAACAGCACCAGCGTTAAGCCATCCACGCCGAATCCCGCCAGGCTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGATGGCGatca                                                                     >  1:781482/1‑140 (MQ=255)

                                                                  aCCAATAAAGCTGGCGATACCAAAACTCAACAGCACCAGCGTTAAGCCATCCACGCCGAATCCCGCCAGGCTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGATGGCGatca                                                                     <  2:781482/140‑1 (MQ=255)

                                                                            cTGGCGATACCAAAACTCAACAGCACCAGCGTTAAGCCATCCACGCCGAATCCCGCCAGGCTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGATGGCGATCATCCCTGCCAt                                                           <  1:181314/140‑1 (MQ=255)

                                                                            cTGGCGATACCAAAACTCAACAGCACCAGCGTTAAGCCATCCACGCCGAATCCCGCCAGGCTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGATGGCGATCATCCCTGCCAt                                                           >  1:694210/1‑140 (MQ=255)

                                                                              ggCGATACCAAAACTCAACAGCACCAGCGTTAAGCCATCCACGCCGAATCCCGCCAGGCTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGATGGCGATCATCCCTGCCATca                                                         >  1:400191/1‑140 (MQ=255)

                                                                                  aTACCAAAACTCAACAGCACCAGCGTTAAGCCATCCACGCCGAATCCCGCCAGGCTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGATGGCGATCATCCCTGCCATCACAcc                                                     <  1:11301/140‑1 (MQ=255)

                                                                                   tACCAAAACTCAACAGCACCAGCGTTAAGCCATCCACGCCGAATCCCGCCAGGCTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGATGGCGATCATCCCTGCCATCACAccc                                                    <  1:35607/140‑1 (MQ=255)

                                                                                     ccAAAACTCAACAGCACCAGCGTTAAGCCATCCACGCCGAATCCCGCCAGGCTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGATGGCGATCATCCCTGCCATCACACCCgg                                                  <  2:75119/140‑1 (MQ=255)

                                                                                       aaaaCTCAACAGCACCAGCGTTAAGCCATCCACGCCGAATCCCGCCAGGCTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGATGGCGATCATCCCTGCCATCACACCCGGAc                                                <  1:143329/140‑1 (MQ=255)

                                                                                        aaaCTCAACAGCACCAGCGTTAAGCCATCCACGCCGAATCCCGCCAGGCTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGATGGCGATCATCCCTGCCATCACACCCGGACg                                               <  2:850986/140‑1 (MQ=255)

                                                                                                   cACCAGCGTTAAGCCATCCACGCCGAATCCCGCCAGGCTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGATGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGc                                    >  1:882106/1‑140 (MQ=255)

                                                                                                     ccAGCGTTAAGCCATCCACGCCGAATCCCGCCAGGCTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGATGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCgg                                  >  2:934200/1‑140 (MQ=255)

                                                                                                        gCGTTAAGCCATCCACGCCGAATCCCGCCAGGCTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGATGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGaaa                               >  1:718640/1‑140 (MQ=255)

                                                                                                           ttAAGCCATCCACGCCGAATCCCGCCAGGCTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGATGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTa                            >  2:237839/1‑140 (MQ=255)

                                                                                                           ttAAGCCATCCACGCCGAATCCCGCCAGGCTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGATGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTa                            >  1:215274/1‑140 (MQ=255)

                                                                                                            tAAGCCATCCACGCCGAATCCCGCCAGGCTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGATGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTAt                           <  2:400191/140‑1 (MQ=255)

                                                                                                              aGCCATCCACGCCGAATCCCGCCAGGCTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGATGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTAttt                         <  2:718640/140‑1 (MQ=255)

                                                                                                                      aCGCCGAATCCCGCCAGGCTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGATGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTg                 <  1:182574/140‑1 (MQ=255)

                                                                                                                      aCGCCGAATCCCGCCAGGCTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGATGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTg                 <  2:882106/140‑1 (MQ=255)

                                                                                                                              tCCCGCCAGGCTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGATGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAgg         >  1:326845/1‑140 (MQ=255)

                                                                                                                                  gCCAGGCTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGATGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTc     >  1:28293/1‑140 (MQ=255)

                                                                                                                                     aGGCTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGATGGCGATCATCCCTGCCATCACGCCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGcc  <  2:660907/140‑1 (MQ=255)

TATTAACGGCGCGCCTGCTAAGGCCAGTTTTACCGAACGTTTAAGAATGAACGACGAAAGCGACGTACCAATAAAGCTGGCGATACCAAAACTCAACAGCACCAGCGTTAAGCCATCCACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGATGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCC  >  NC_000913/3840870‑3841142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A