Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	155	60.3	2039550	99.3%	2025273	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,928,843	C→T	G254D (GGT→GAT)	viaA ←	protein ViaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,928,843	0	C	T	100.0%	167.8 / NA	47	G254D (GGT→GAT)	viaA	protein ViaA
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (29/18); total (29/18)

TCACCGTGCAGGCGATAGGTGAGCAACTGTTTTTCCACCAGCCGACGGTAAAACTCATACTCCAGTTCCGTTATCCCTAGTGTCGCCAGTTCTGGCGGCAGGAGACGTAAAATATCATCGCTTTGTTGCAGACCATCAACCTGCTCAGGAACCGTCGCCGGTTCGCGCACCATGGTGCGGAAGGTTTCCATCTGCGCATCGTTGCGCGGTATTGATTTGGCTTCCCGAGAACGCCCCAGCTGCTCTGCCAGGCGTTTCAGTTCCGGCTG  >  NC_000913/3928711‑3928979

tCACCGTGCAGGCGATAGGTGAGCAACTGTTTTTCCACCAGCCGACGGTAAAACTCATACTCCAGTTCCGTTATCCCTAGTGTCGCCAGTTCTGGCGGCAGGAGACGTAAAATATCATCGCTTTGTTGCAGATCATCAAc                                                                                                                                   <  1:692809/140‑1 (MQ=255)

         aGGCGATAGGTGAGCAACTGTTTTTCCACCAGCCGACGGTAAAACTCATACTCCAGTTCCGTTATCCCTAGTGTCGCCAGTTCTGGCGGCAGGAGACGTAAAATATCATCGCTTTGTTGCAGATCATCAACCTGCTCAgg                                                                                                                          >  2:724631/1‑140 (MQ=255)

            cGATAGGTGAGCAACTGTTTTTCCACCAGCCGACGGTAAAACTCATACTCCAGTTCCGTTATCCCTAGTGTCGCCAGTTCTGGCGGCAGGAGACGTAAAATATCATCGCTTTGTTGCAGATCATCAACCTGCTCAGGAAc                                                                                                                       <  1:114972/140‑1 (MQ=255)

              aTAGGTGAGCAACTGTTTTTCCACCAGCCGACGGTAAAACTCATACTCCAGTTCCGTTATCCCTAGTGTCGCCAGTTCTGGCGGCAGGAGACGTAAAATATCATCGCTTTGTTGCAGATCATCAACCTGCTCAGGAACCg                                                                                                                     >  1:412489/1‑140 (MQ=255)

                aGGTGAGCAACTGTTTTTCCACCAGCCGACGGTAAAACTCATACTCCAGTTCCGTTATCCCTAGTGTCGCCAGTTCTGGCGGCAGGAGACGTAAAATATCATCGCTTTGTTGCAGATCATCAACCTGCTCAGGAACCGTc                                                                                                                   >  1:89077/1‑140 (MQ=255)

                aGGTGAGCAACTGTTTTTCCACCAGCCGACGGTAAAACTCATACTCCAGTTCCGTTATCCCTAGTGTCGCCAGTTCTGGCGGCAGGAGACGTAAAATATCATCGCTTTGTTGCAGATCATCAACCTGCTCAGGAACCGTc                                                                                                                   >  2:341648/1‑140 (MQ=255)

                     aGCAACTGTTTTTCCACCAGCCGACGGTAAAACTCATACTCCAGTTCCGTTATCCCTAGTGTCGCCAGTTCTGGCGGCAGGAGACGTAAAATATCATCGCTTTGTTGCAGATCATCAACCTGCTCAGGAACCGTCGCCgg                                                                                                              >  1:111822/1‑140 (MQ=255)

                                ttCCACCAGCCGACGGTAAAACTCATACTCCAGTTCCGTTATCCCTAGTGTCGCCAGTTCTGGCGGCAGGAGACGTAAAATATCATCGCTTTGTTGCAGATCATCAACCTGCTCAGGAACCGTCGCCGGTTCGCGCACCa                                                                                                   <  1:724631/140‑1 (MQ=255)

                                 tCCACCAGCCGACGGTAAAACTCATACTCCAGTTCCGTTATCCCTAGTGTCGCCAGTTCTGGCGGCAGGAGACGTAAAATATCATCGCTTTGTTGCAGATCATCAACCTGCTCAGGAACCGTCGCCGGTTCGCGCACCAt                                                                                                  <  2:89077/140‑1 (MQ=255)

                                 tCCACCAGCCGACGGTAAAACTCATACTCCAGTTCCGTTATCCCTAGTGTCGCCAGTTCTGGCGGCAGGAGACGTAAAATATCATCGCTTTGTTGCAGATCATCAACCTGCTCAGGAACCGTCGCCGGTTCGCGCACCAt                                                                                                  <  2:412489/140‑1 (MQ=255)

                                     ccaGCCGACGGTAAAACTCATACTCCAGTTCCGTTATCCCTAGTGTCGCCAGTTCTGGCGGCAGGAGACGTAAAATATCATCGCTTTGTTGCAGATCATCAACCTGCTCAGGAACCGTCGCCGGTTCGCGCACCATGGTg                                                                                              <  1:316204/140‑1 (MQ=255)

                                      caGCCGACGGTAAAACTCATACTCCAGTTCCGTTATCCCTAGTGTCGCCAGTTCTGGCGGCAGGAGACGTAAAATATCATCGCTTTGTTGCAGATCATCAACCTGCTCAGGAACCGTCGCCGGTTCGCGCACCATGGTGc                                                                                             >  2:927407/1‑140 (MQ=255)

                                       aGCCGACGGTAAAACTCATACTCCAGTTCCGTTATCCCTAGTGTCGCCAGTTCTGGCGGCAGGAGACGTAAAATATCATCGCTTTGTTGCAGATCATCAACCTGCTCAGGAACCGTCGCCGGTTCGCGCACCATGGTGCg                                                                                            >  1:783396/1‑140 (MQ=255)

                                        gCCGACGGTAAAACTCATACTCCAGTTCCGTTATCCCTAGTGTCGCCAGTTCTGGCGGCAGGAGACGTAAAATATCATCGCTTTGTTGCAGATCATCAACCTGCTCAGGAACCGTCGCCGGTTCGCGCACCATGGTGCgg                                                                                           >  2:361648/1‑140 (MQ=255)

                                         ccGACGGTAAAACTCATACTCCAGTTCCGTTATCCCTAGTGTCGCCAGTTCTGGCGGCAGGAGACGTAAAATATCATCGCTTTGTTGCAGATCATCAACCTGCTCAGGAACCGTCGCCGGTTCGCGCACCATGGTGCGGa                                                                                          <  1:341648/140‑1 (MQ=255)

                                                     cTCATACTCCAGTTCCGTTATCCCTAGTGTCGCCAGTTCTGGCGGCAGGAGACGTAAAATATCATCGCTTTGTTGCAGATCATCAACCTGCTCAGGAACCGTCGCCGGTTCGCGCACCATGGTGCGGAAGGTTTCCATCt                                                                              <  1:361648/140‑1 (MQ=255)

                                                          aCTCCAGTTCCGTTATCCCTAGTGTCGCCAGTTCTGGCGGCAGGAGACGTAAAATATCATCGCTTTGTTGCAGATCATCAACCTGCTCAGGAACCGTCGCCGGTTCGCGCACCATGGTGCGGAAGGTTTCCATCTGCGCa                                                                         >  1:592152/1‑140 (MQ=255)

                                                             ccAGTTCCGTTATCCCTAGTGTCGCCAGTTCTGGCGGCAGGAGACGTAAAATATCATCGCTTTGTTGCAGATCATCAACCTGCTCAGGAACCGTCGCCGGTTCGCGCACCATGGTGCGGAAGGTTTCCATCTGCGCATCg                                                                      <  1:41151/140‑1 (MQ=255)

                                                              cAGTTCCGTTATCCCTAGTGTCGCCAGTTCTGGCGGCAGGAGACGTAAAATATCATCGCTTTGTTGCAGATCATCAACCTGCTCAGGAACCGTCGCCGGTTCGCGCACCATGGTGCGGAAGGTTTCCATCTGCGCATCGt                                                                     >  2:976440/1‑140 (MQ=255)

                                                                      ttATCCCTAGTGTCGCCAGTTCTGGCGGCAGGAGACGTAAAATATCATCGCTTTGTTGCAGATCATCAACCTGCTCAGGAACCGTCGCCGGTTCGCGCACCATGGTGCGGAAGGTTTCCATCTGCGCa                                                                         >  2:698713/1‑128 (MQ=255)

                                                                      ttATCCCTAGTGTCGCCAGTTCTGGCGGCAGGAGACGTAAAATATCATCGCTTTGTTGCAGATCATCAACCTGCTCAGGAACCGTCGCCGGTTCGCGCACCATGGTGCGGAAGGTTTCCATCTGCGCa                                                                         <  1:698713/128‑1 (MQ=255)

                                                                        aTCCCTAGTGTCGCCAGTTCTGGCGGCAGGAGACGTAAAATATCATCGCTTTGTTGCAGATCATCAACCTGCTCAGGAACCGTCGCCGGTTCGCGCACCATGGTGCGGAAGGTTTCCATCTGCGCATCGTTGCGCGGTAt                                                           >  1:293156/1‑140 (MQ=255)

                                                                         tCCCTAGTGTCGCCAGTTCTGGCGGCAGGAGACGTAAAATATCATCGCTTTGTTGCAGATCATCAACCTGCTCAGGAACCGTCGCCGGTTCGCGCACCATGGTGCGGAAGGTTTCCATCTGCGCATCGTTGCGCGGTAtt                                                          <  2:994400/140‑1 (MQ=255)

                                                                              aGTGTCGCCAGTTCTGGCGGCAGGAGACGTAAAATATCATCGCTTTGTTGCAGATCATCAACCTGCTCAGGAACCGTCGCCGGTTCGCGCACCATGGTGCGGAAGGTTTCCATCTGCGCATCGTTGCGCGGTATTGAttt                                                     >  2:789872/1‑140 (MQ=255)

                                                                                   cGCCAGTTCTGGCGGCAGGAGACGTAAAATATCATCGCTTTGTTGCAGATCATCAACCTGCTCAGGAACCGTCGCCGGTTCGCGCACCATGGTGCGGAAGGTTTCCATCTGCGCATCGTTGCGCGGTATTGATTTGGCtt                                                >  1:198847/1‑140 (MQ=255)

                                                                                    gCCAGTTCTGGCGGCAGGAGACGTAAAATATCATCGCTTTGTTGCAGATCATCAACCTGCTCAGGAACCGTCGCCGGTTCGCGCACCATGGTGCGGAAGGTTTCCATCTGCGCATCGTTgcg                                                                 >  1:966227/1‑122 (MQ=255)

                                                                                    gCCAGTTCTGGCGGCAGGAGACGTAAAATATCATCGCTTTGTTGCAGATCATCAACCTGCTCAGGAACCGTCGCCGGTTCGCGCACCATGGTGCGGAAGGTTTCCATCTGCGCATCGTTgcg                                                                 <  2:966227/122‑1 (MQ=255)

                                                                                     ccAGTTCTGGCGGCAGGAGACGTAAAATATCATCGCTTTGTTGCAGATCATCAACCTGCTCAGGAACCGTCGCCGGTTCGCGCACCATGGTGCGGAAGGTTTCCATCTGCGCATCGTTgcgc                                                                >  2:258643/1‑122 (MQ=255)

                                                                                     ccAGTTCTGGCGGCAGGAGACGTAAAATATCATCGCTTTGTTGCAGATCATCAACCTGCTCAGGAACCGTCGCCGGTTCGCGCACCATGGTGCGGAAGGTTTCCATCTGCGCATCGTTgcgc                                                                <  1:258643/122‑1 (MQ=255)

                                                                                     ccAGTTCTGGCGGCAGGAGACGTAAAATATCATCGCTTTGTTGCAGATCATCAACCTGCTCAGGAACCGTCGCCGGTTCGCGCACCATGGTGCGGAAGGTTTCCATCTGCGCATCGTTGCGCGGTATTGATTTGGc                                                  <  1:537837/136‑1 (MQ=255)

                                                                                     ccAGTTCTGGCGGCAGGAGACGTAAAATATCATCGCTTTGTTGCAGATCATCAACCTGCTCAGGAACCGTCGCCGGTTCGCGCACCATGGTGCGGAAGGTTTCCATCTGCGCATCGTTGCGCGGTATTGATTTGGc                                                  >  2:537837/1‑136 (MQ=255)

                                                                                     ccAGTTCTGGCGGCAGGAGACGTAAAATATCATCGCTTTGTTGCAGATCATCAACCTGCTCAGGAACCGTCGCCGGTTCGCGCACCATGGTGCGGAAGGTTTCCATCTGCGCATCGTTGCGCGGTATTGATTTGGCTTcc                                              >  1:201749/1‑140 (MQ=255)

                                                                                         ttCTGGCGGCAGGAGACGTAAAATATCATCGCTTTGTTGCAGATCATCAACCTGCTCAGGAACCGTCGCCGGTTCGCGCACCATGGTGCGGAAGGTTTCCATCTGCGCATCGTTGCGCGGTATTGATTTGGCTTCCCgag                                          <  1:789872/140‑1 (MQ=255)

                                                                                         ttCTGGCGGCAGGAGACGTAAAATATCATCGCTTTGTTGCAGATCATCAACCTGCTCAGGAACCGTCGCCGGTTCGCGCACCATGGTGCGGAAGGTTTCCATCTGCGCATCGTTGCGCGGTATTGATTTGGCTTCCCgag                                          >  1:809005/1‑140 (MQ=255)

                                                                                                   aGGAGACGTAAAATATCATCGCTTTGTTGCAGATCATCAACCTGCTCAGGAACCGTCGCCGGTTCGCGCACCATGGTGCGGAAGGTTTCCATCTGCGCATCGTTGCGCGGTATTGATTTGGCTTCCCGAGAACGCCCCAg                                >  1:207422/1‑140 (MQ=255)

                                                                                                   aGGAGACGTAAAATATCATCGCCTTGTTGCAGATCATCAACCTGCTCAGGAACCGTCGCCGGTTCGCGCACCATGGTGCGGAAGGTTTCCATCTGCGCATCGTTGCGCGGTATTGATTTGGCTTCCCGAGAACGCCCCAg                                >  1:385882/1‑140 (MQ=255)

                                                                                                       gaCGTAAAATATCATCGCTTTGTTGCAGATCATCAACCTGCTCAGGAACCGTCGCCGGTTCGCGCACCATGGTGCGGAAGGTTTCCATCTGCGCATCGTTGCGCGGTATTGATTTGGCTTCCCGAGAACGCCCCAgctgc                            <  1:927407/140‑1 (MQ=255)

                                                                                                             aaaTATCATCGCTTTGTTGCAGATCATCAACCTGCTCAGGAACCGTCGCCGGTTCGCGCACCATGGTGCGGAAGGTTTCCATCTGCGCATCGTTGCGCGGTATTGATTTGGCTTCCCGAGAACGCCCCAGCTGCTCTGcc                      <  1:931901/140‑1 (MQ=255)

                                                                                                               atatCATCGCTTTGTTGCAGATCATCAACCTGCTCAGGAACCGTCGCCGGTTCGCGCACCATGGTGCGGAAGGTTTCCATCTGCGCATCGTTGCGCGGTATTGATTTGGCTTCCCGAGAACGCCCCAGCTGCTCTGCCAg                    >  1:463781/1‑140 (MQ=255)

                                                                                                                  tcatcGCTTTGTTGCAGATCATCAACCTGCTCAGGAACCGTCGCCGGTTCGCGCACCATGGTGCGGAAGGTTTCCATCTGCGCATCGTTGCGCGGTATTGATTTGGCTTCCCGAGAACGCCACAGCTGCTCTGCCAGGCg                 >  1:648429/1‑140 (MQ=255)

                                                                                                                    atcGCTTTGTTGCAGATCATCAACCTGCTCAGGAACCGTCGCCGGTTCGCGCACCATGGTGCGGAAGGTTTCCATCTGCGCATCGTTGCGCGGTATTGATTTGGCTTCCCGAGAACGCCCCAGCTGCTCTGCCAGGCGtt               <  2:463781/140‑1 (MQ=255)

                                                                                                                     tcGCTTTGTTGCAGATCATCAACCTGCTCAGGAACCGTCGCCGGTTCGCGCACCATGGTGCGGAAGGTTTCCATCTGCGCATCGTTGCGCGGTATTGATTTGGCTTCCCGAGAACGCCCCAGCTGCTCTGCCAGGCGttt              >  2:353016/1‑140 (MQ=255)

                                                                                                                      cGCTTTGTTGCAGATCATCAACCTGCTCAGGAACCGTCGCCGGTTCGCGCACCATGGTGCGGAAGGTTTCCATCTGCGCATCGTTGCGCGGTATTGATTTGGCTTCCCGAGAACGCCCCAGCTGCTCTGCCAGGCGTTTc             >  2:387582/1‑140 (MQ=255)

                                                                                                                        cTTTGTTGCAGATCATCAACCTGCTCAGGAACCGTCGCCGGTTCGCGCACCATGGTGCGGAAGGTTTCCATCTGCGCATCGTTGCGCGGTATTGA‑TTGGCTTCCCGAGAACGCCCCAGCTGCTCTGCCAGGCGTTTCAGt          >  2:483374/1‑140 (MQ=255)

                                                                                                                          ttgttgCAGATCATCAACCTGCTCAGGAACCGTCGCCGGTTCGCGCACCATGGTGCGGAAGGTTTCCATCTGCGCATCGTTGCGCGGTATTGATTTGGCTTCCCGAGAACGCCCCAGCTGCTCTGCCAGGCGTTTCAGtt         >  1:355352/1‑140 (MQ=255)

                                                                                                                            gttgCAGATCATCAACCTGCTCAGGAACCGTCGCCGGTTCGCGCACCATGGTGCGGAAGGTTTCCATCTGCGCATCGTTGCGCGGTATTGATTTGGCTTCCCGAGAACGCCCCAGCTGCTCTGCCAGGCGTTTCAGTTcc       >  1:874370/1‑140 (MQ=255)

                                                                                                                             ttgCAGATCATCAACCTGCTCAGGAACCGTCGCCGGTTCGCGCACCATGGTGCGGAAGGTTTCCATCTGCGCATCGTTGCGCGGTATTGATTTGGCTTCCCGAGAACGCCCCAGCTGCTCTGCCAGGCGTTTCAGTTCCg      >  1:823519/1‑140 (MQ=255)

                                                                                                                                 aGATCATCAACCTGCTCAGGAACCGTCGCCGGTTCGCGCACCATGGTGCGGAAGGTTTCCATCTGCGCATCGTTGCGCGGTATTGATTTGGCTTCCCGAGAACGCCCCAGCTGCTCTGCCAGGCGTTTCAGTTCCGGCTg  <  2:592152/140‑1 (MQ=255)

TCACCGTGCAGGCGATAGGTGAGCAACTGTTTTTCCACCAGCCGACGGTAAAACTCATACTCCAGTTCCGTTATCCCTAGTGTCGCCAGTTCTGGCGGCAGGAGACGTAAAATATCATCGCTTTGTTGCAGACCATCAACCTGCTCAGGAACCGTCGCCGGTTCGCGCACCATGGTGCGGAAGGTTTCCATCTGCGCATCGTTGCGCGGTATTGATTTGGCTTCCCGAGAACGCCCCAGCTGCTCTGCCAGGCGTTTCAGTTCCGGCTG  >  NC_000913/3928711‑3928979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A