Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F28 I1 R1 155 60.3 2039550 99.3% 2025273 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,095,004 A→G F327L (TTC→CTC)  rhaA ← L‑rhamnose isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,095,0040AG98.4% 216.3 / ‑4.2 63F327L (TTC→CTC) rhaAL‑rhamnose isomerase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (31/31);  total (31/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.64e-01

GCGCAGTGTAATCGCCCGCCGCTTCCAGCTTGCGCAGCTCAGCGGTAGGTTCCAGCAACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAAGTCAAGGCCGATATGCACCCGGTCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGAACCGGACGGCTGACGTGCAGCAGCAACT  >  NC_000913/4094869‑4095138
                                                                                                                                       |                                                                                                                                      
gcgcAGTGTAATCGCCCGCCGCTTCCAGCTTGCGCAGCTCAGCGGTAGGTTCCAGCAACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTCa                                                                                                                                    <  2:746741/140‑1 (MQ=255)
 cgcAGTGTAATCGCCCGCCGCTTCCAGCTTGCGCAGCTCAGCGGTAGGTTCCAGCAACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTCaa                                                                                                                                   <  2:5307/140‑1 (MQ=255)
  gcAGTGTAATCGCCCGCCGCTTCCAGCTTGCGCAGCTCAGCGGTAGGTTCCAGCAACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAg                                                                                                                                  >  2:65984/1‑140 (MQ=255)
  gcAGTGTAATCGCCCGCCGCTTCCAGCTTGCGCAGCTCAGCGGTAGGTTCCAGCAACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAg                                                                                                                                  >  1:914950/1‑140 (MQ=255)
    aGTGTAATCGCCCGCCGCTTCCAGCTTGCGCAGCTCAGCGGTAGGTTCCAGCAACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAGGc                                                                                                                                <  1:615801/140‑1 (MQ=255)
    aGTGTAATCGCCCGCCGCTTCCAGCTTGCGCAGCTCAGCGGTAGGTTCCAGCAACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAGGc                                                                                                                                <  2:914950/140‑1 (MQ=255)
    aGTGTAATCGCCCGCCGCTTCCAGCTTGCGCAGCTCAGCGGTAGGTTCCAGCAACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAGGc                                                                                                                                <  2:800005/140‑1 (MQ=255)
    aGTGTAATCGCCCGCCGCTTCCAGCTTGCGCAGCTCAGCGGTAGGTTCCAGCAACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAAGTCAAGGc                                                                                                                                <  2:668309/140‑1 (MQ=255)
       gtAATCGCCCGCCGCTTCCAGCTTGCGCAGCTCAGCGGTAGGTTCCAGCAACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAGGCCGa                                                                                                                             <  1:497442/140‑1 (MQ=255)
             gcccgccGCTTCCAGCTTGCGCAGCTCAGCGGTAGGTTCCAGCAACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAGGCCGATATGCa                                                                                                                       <  1:64930/140‑1 (MQ=255)
             gcccgccGCTTCCAGCTTGCGCAGCTCAGCGGTAGGTTCCAGCAACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAGGCCGATATACa                                                                                                                       <  2:338303/140‑1 (MQ=255)
              cccgccGCTTCCAGCTTGCGCAGCTCAGCGGTAGGTTCCAGCAACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTc                                                                                                                                     <  1:243475/125‑1 (MQ=255)
              cccgccGCTTCCAGCTTGCGCAGCTCAGCGGTAGGTTCCAGCAACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTc                                                                                                                                     >  2:243475/1‑125 (MQ=255)
               ccgccgCTTCCAGCTTGCGCAGCTCAGCGGTAGGTTCCAGCAACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAGGCCGATATGCAcc                                                                                                                     <  1:812016/140‑1 (MQ=255)
                cgccgcTTCCAGCTTGCGCAGCTCAGCGGTAGGTTCCAGCAACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAGGCCGATATGCAccc                                                                                                                    >  2:510425/1‑140 (MQ=255)
                cgccgcTTCCAGCTTGCGCAGCTCAGCGGTAGGTTCCAGCAACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAGGCCGATATGCAccc                                                                                                                    >  1:839419/1‑140 (MQ=255)
                cgccgcTTCCAGCTTGCGCAGCTCAGCGGTAGGTTCCAGCAACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAGGCCGATATGCAccc                                                                                                                    >  1:233063/1‑140 (MQ=255)
                cgccgcTTCCAGCTTGCGCAGCTCAGCGGTAGGTTCCAGCAACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAGGCCGATATGCAccc                                                                                                                    >  1:189665/1‑140 (MQ=255)
                 gccgcTTCCAGCTTGCGCAGCTCAGCGGTAGGTTCCAGCAACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAGGCCGATATGCACCCg                                                                                                                   <  1:873723/140‑1 (MQ=255)
                  ccgcTTCCAGCTTGCGCAGCTCAGCGGTAGGTTCCAGCAACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAGGCCGATATGCACCCgg                                                                                                                  >  1:183954/1‑140 (MQ=255)
                               gcgcAGCTCAGCGGTAGGTTCCAGCAACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAAGGACCCACGCGGCAATGCCGTTGATAGAGGCATCGAAGAGGTCAAGTCCGATATGCACCCTGTCAAACAGATCGt                                                                                                     <  2:215363/140‑1 (MQ=255)
                                     cTCAGTGGTAGGTTCCAGCAACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAGGCCGATATGCACCCGGTCAAACAGATCGTGACGca                                                                                               <  1:278308/140‑1 (MQ=255)
                                              aGGTTCCAGCAACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAGGCCGATATGCACCCGGTCAAACAGATCGTGACGCACAATCTCAc                                                                                      <  2:183954/140‑1 (MQ=255)
                                                    cAGCAACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAGGCCGATATGCACCCGGTCAAACAGATCGTGACGCACAATCTCACTGGCaa                                                                                >  2:10035/1‑140 (MQ=255)
                                                    cAGCAACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAGGCCGATATGCACCCGGTCAAACAGATCGTGACGCACAATCTCACTGGCaa                                                                                >  2:474413/1‑140 (MQ=255)
                                                       cAACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAGGCCGATATGCACCCGGTCAAACAGATCGTGACGCACAATCTCACTGGCAATTg                                                                             >  2:345899/1‑140 (MQ=255)
                                                        aaCGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAGGCCGATATGCACCCGGTCAAACAGATCGTGACGCACAATCTCACTGGCAATTGc                                                                            <  1:510425/140‑1 (MQ=255)
                                                        aaCGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAGGCCGATATGCACCCGGTCAAACAGATCGTGACGCACAATCTCACTGGCAATTGc                                                                            <  1:294992/140‑1 (MQ=255)
                                                           gcacgcaGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAGGCCGATATGCACCCGGTCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTg                                                                         >  1:680153/1‑140 (MQ=255)
                                                                  gcagGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAGGCCGATATGCACCCGGTCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTtca                                                                  <  2:319783/140‑1 (MQ=255)
                                                                  gcagGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAGGCCGATATGCACCCGGTCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTtca                                                                  <  1:10035/140‑1 (MQ=255)
                                                                      ggCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAGGCCGATATGCACCCGGTCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTcatcat                                                              >  1:510343/1‑140 (MQ=255)
                                                                        cTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAGGCCGATATGCACCCGGTCAAACAGATCATGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATcc                                                            <  1:370847/140‑1 (MQ=255)
                                                                         ttttttCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAGGCCGATATGCACCCGGTCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCca                                                           <  2:510343/140‑1 (MQ=255)
                                                                             ttCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAGGCCGATATGCACCCGGTCAAACAGATTGTGACGCACAATCTCACTGGTAATTGCCTGGGTTTCATCATCcagcag                                                       <  1:534897/140‑1 (MQ=255)
                                                                             ttCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAGGCCGATATGCACCCGGTCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCcagcag                                                       <  1:65984/140‑1 (MQ=255)
                                                                             ttCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAGGCCGATATGCACCCGGTCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCcagcag                                                       <  2:189665/140‑1 (MQ=255)
                                                                             ttCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAGGCCGATATGCACCCGGTCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCcagcag                                                       <  2:839419/140‑1 (MQ=255)
                                                                             ttCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAAGCCGATATGCACCCGGTCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCcagcag                                                       >  2:698056/1‑140 (MQ=255)
                                                                             ttCATAGTGCGTGTTCTAATGGCCGACCCGGCAAGGCGGTTGATAGAGGCATCGATGAGGTCAAGGCGGGTATACACCCGGTCAAACAGACCGGGGCGCCCACTCTCACGGGCAATGGCCTGGGCTTCATGCTCcagcag                                                       <  2:233063/140‑1 (MQ=255)
                                                                                atatTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAGGCCGATATGCACCCGGTCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCAc                                                    <  2:680153/140‑1 (MQ=255)
                                                                                atatTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAGGCCGATATGCACCCGGTCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCAc                                                    <  2:848770/140‑1 (MQ=255)
                                                                                  atTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAGGCCGATATGCACCCGGTCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTa                                                  >  1:10501/1‑140 (MQ=255)
                                                                                      cGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAGGCCGATATGCACCCGGTCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTg                                              >  1:255853/1‑140 (MQ=255)
                                                                                       gtgtACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAGGCCGATATGCACCCGGTCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTtcatca                                                               <  2:457550/122‑1 (MQ=255)
                                                                                       gtgtACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAGGCCGATATGCACCCGGTCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTtcatca                                                               >  1:457550/1‑122 (MQ=255)
                                                                                        tgtACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAGGCCGATATGCACCCGGTCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGAt                                            >  1:824882/1‑140 (MQ=255)
                                                                                        tgtACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAGGCCGATATGCACCCGGTCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGAt                                            >  2:552814/1‑140 (MQ=255)
                                                                                             cAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAGGCCGATATGCACCCGGTCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTg                                       <  2:824882/140‑1 (MQ=255)
                                                                                                tGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAGGCCGATATGCACCCGGTCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTcc                                    >  1:592743/1‑140 (MQ=255)
                                                                                                tGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAGGCCGATATGCACCCGGTCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTcc                                    >  2:130007/1‑140 (MQ=255)
                                                                                                  aCCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAGGCCGATATGCACCCGGTCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCa                                  >  2:861531/1‑140 (MQ=255)
                                                                                                     cACGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAGGCCGATATGCACCCGGTCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCg                               >  1:975908/1‑140 (MQ=255)
                                                                                                      aCGCGGCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAGGCCGATATGCACCCGGTCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGa                              >  2:1003321/1‑140 (MQ=255)
                                                                                                       cgcgGCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAGGCCGATATGCACCCGGTCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGaa                             >  2:254788/1‑140 (MQ=255)
                                                                                                       cgcgGCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAGGCCGATATGCACCCGGTCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGaa                             >  2:285787/1‑140 (MQ=255)
                                                                                                           gCAATGCGGTTGATAGAGGCATCGAAGAGGTCAAGGCCGATATGCACCCGGTCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGAACCgg                         <  2:592743/140‑1 (MQ=255)
                                                                                                               tGCGGTTGATAGAGGCATCGAAGAGGTCAAGGCCGATATGCACCCGGTCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGaa                             >  2:262165/1‑132 (MQ=255)
                                                                                                               tGCGGTTGATAGAGGCATCGAAGAGGTCAAGGCCGATATGCACCCGGTCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGaa                             <  1:262165/132‑1 (MQ=255)
                                                                                                                           agGCATCGAAGAGGTCAAGGCCGATATGCACCCGGTCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGAACCGGAc                       <  2:525997/126‑1 (MQ=255)
                                                                                                                           agGCATCGAAGAGGTCAAGGCCGATATGCACCCGGTCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGAACCGGAc                       >  1:525997/1‑126 (MQ=255)
                                                                                                                           agGCATCGAAGAGGTCAAGGCCGATATGCACCCGGTCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGAACCGGACGGCTGACGTgcagc         >  2:447203/1‑140 (MQ=255)
                                                                                                                             gcttCGAAGAGGTCAAGGCCGATATGCACCCGGTCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGAACCGGACGGCTGACGTgcagcag       >  2:94543/4‑140 (MQ=255)
                                                                                                                              cATCGAAGAGGTCAAGGCCGATATGCACCCGGTCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGAACCGGACGGCTGACGTgcagcagc      >  2:32999/1‑140 (MQ=255)
                                                                                                                                  gaagagGTCAAGGCCGATATGCACCCGGTCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGAACCGGACGGCTGACGTGCAGCAGCAACt  <  1:254788/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                      
GCGCAGTGTAATCGCCCGCCGCTTCCAGCTTGCGCAGCTCAGCGGTAGGTTCCAGCAACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAAGTCAAGGCCGATATGCACCCGGTCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGAACCGGACGGCTGACGTGCAGCAGCAACT  >  NC_000913/4094869‑4095138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A