Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F28 I1 R1 155 60.3 2039550 99.3% 2025273 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,173,359 A→G Y93C (TAC→TGC)  birA → DNA‑binding transcriptional repressor/biotin‑[acetyl‑CoA‑carboxylase] ligase BirA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,173,3590AG100.0% 188.2 / NA 53Y93C (TAC→TGC) birADNA‑binding transcriptional repressor/biotin‑[acetyl‑CoA‑carboxylase] ligase BirA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/30);  total (23/30)

TGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAGTGATTGACTCCACGAATCAGTACCTTCTTGATCGTATCGGAGAGCTTAAATCGGGCGATGCTTGCATTGCAGAATACCAGCAGGCTGGCCGTGGTCGCCGGGGTCGGAAATGGTTTTCGCCTTTTGGCGCAAACTTATATTTGTCGATGTTCTG  >  NC_000913/4173225‑4173491
                                                                                                                                      |                                                                                                                                    
tGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGTTGGCGGTAGTGTAGCCGTGCTGCCAGTGATTGACTCCACGAATCAGTGCcttc                                                                                                                                 >  2:772078/1‑140 (MQ=255)
tGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAGTGATTGACTCCACGAATCAGTGCcttc                                                                                                                                 <  1:853023/140‑1 (MQ=255)
 gATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAGTGATTGACTCCACGAATCAGTGCcttct                                                                                                                                >  1:739432/1‑140 (MQ=255)
    gTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAGTGATTGACTCCACGAATCAGTGCCTTCTTGa                                                                                                                             <  1:554595/140‑1 (MQ=255)
     tCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAGTGATTGACTCCACGAATCAGTGCCTTCTTGAt                                                                                                                            >  1:803746/1‑140 (MQ=255)
         tACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAGTGATTGACTCCACGAATCAGTGCCTTCTTGATCGTa                                                                                                                        <  2:589472/140‑1 (MQ=255)
            cGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAGTGATTGACTCCACGAATCAGTGCCTTCTTGATCGTATCg                                                                                                                     <  1:559844/140‑1 (MQ=255)
             gTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAGTGATTGACTCCACGAATCAGTGCCTTCTTGATCGTATCgg                                                                                                                    <  2:376908/140‑1 (MQ=255)
               tCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAGTGATTGACTCCACGAATCAGTGCCTTCTTGATCGTATCGgag                                                                                                                  >  1:828235/1‑140 (MQ=255)
               tCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAGTGATTGACTCCACGAATCAGTGCCTTCTTGATCGTATCGgag                                                                                                                  >  2:897544/1‑140 (MQ=255)
               tCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAGTGATTGACTCCACGAATCAGTGCCTTCTTGATCGTATCGgag                                                                                                                  <  2:803746/140‑1 (MQ=255)
                    gTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAGTGATTGACTCCACGAATCAGTGCCTTCTTGATCGTATCGGAGAGCtt                                                                                                             >  1:223205/1‑140 (MQ=255)
                           aTACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAGTGATTGACTCCACGAATCAGTGCCTTCTTGATCGTATCgg                                                                                                                    <  2:288631/126‑1 (MQ=255)
                           aTACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAGTGATTGACTCCACGAATCAGTGCCTTCTTGATCGTATCgg                                                                                                                    >  1:288631/1‑126 (MQ=255)
                             aCAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAGTGATTGACTCCACGAATCAGTGCCTTCTTGATCGTATCGGAGAGCTTAAATCGGGc                                                                                                    <  1:650970/140‑1 (MQ=255)
                             aCAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAGTGATTGACTCCACGAATCAGTGCCTTCTTGATCGTATCGGAGAGCTTAAATCGGGc                                                                                                    <  2:1014519/140‑1 (MQ=255)
                              cAGCCTGCCTGGGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAGTGATTGACTCCACGAATCAGTGCCTTCTTGATCGTATCGGAGAGCTTAAATCGGGCg                                                                                                   >  2:596009/1‑140 (MQ=255)
                              cAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAGTGATTGACTCCACGAATCAGTGCCTTCTTGATCGTATCGGAGAGCTTAAATCGGGCg                                                                                                   <  1:598310/140‑1 (MQ=255)
                                     cctgAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAGTGATTGACTCCACGAATCAGTGCCTTCTTGATCGTATCGGAGAGCTTAAATCGGGCGATGCttg                                                                                            >  1:969021/1‑140 (MQ=255)
                                       tgAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAGTGATTGACTCCACGAATCAGTGCCTTCTTGATCGTATCGGAGAGCTTAAATCGGGCGATGCttgca                                                                                          <  1:756111/140‑1 (MQ=255)
                                        gAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAGTGATTGACTCCACGAATCAGTGCCTTCTTGATCGTATCGGAGAGCTTAAATCGGGCGATGCttgcat                                                                                         <  2:969021/140‑1 (MQ=255)
                                         aGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAGTGATTGACTCCACGAATCAGTGCCTTCTTGATCGTATCGGAGAGCTTAAATCGGGCGATGCttgcatt                                                                                        >  2:160552/1‑140 (MQ=255)
                                           ccTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAGTGATTGACTCCACGAATCAGTGCCTTCTTGATCGTATCGGAGAGCTTAAATCggg                                                                                                     <  2:1016009/125‑1 (MQ=255)
                                           ccTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAGTGATTGACTCCACGAATCAGTGCCTTCTTGATCGTATCGGAGAGCTTAAATCggg                                                                                                     >  1:1016009/1‑125 (MQ=255)
                                           ccTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAGTGATTGACTCCACGAATCAGTGCCTTCTTGATCGTATCGGAGAGCTTAAATCGGGCGATGCttgcattgc                                                                                      <  1:578230/140‑1 (MQ=255)
                                               tCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAGTGATTGACTCCACGAATCAGTGCCTTCTTGATCGTATCGGAGAGCTTAAATCGGGCGATGCttgcattgc                                                                                      >  2:687728/1‑136 (MQ=255)
                                               tCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAGTGATTGACTCCACGAATCAGTGCCTTCTTGATCGTATCGGAGAGCTTAAATCGGGCGATGCttgcattgc                                                                                      <  1:687728/136‑1 (MQ=255)
                                                    ttACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAGTGATTGACTCCACGAATCAGTGCCTTCTTGATCGTATCGGAGAGCTTAAATCGGGCGATGCTTGCATTGCAGaa                                                                                  >  1:897814/1‑135 (MQ=255)
                                                    ttACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAGTGATTGACTCCACGAATCAGTGCCTTCTTGATCGTATCGGAGAGCTTAAATCGGGCGATGCTTGCATTGCAGaa                                                                                  <  2:897814/135‑1 (MQ=255)
                                                    ttACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAGTGATTGACTCCACGAATCAGTGCCTTCTTGATCGTATCGGAGAGCTTAAATCGGGCGATGCTTGCATTGCAGAATACca                                                                             >  1:806337/1‑140 (MQ=255)
                                                     tACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAGTGATTGACTCCACGAATCAGTGCCTTCTTGATCGTATCGGAGAGCTTAAATCGGGCGATGCTTGCATTGCAGAATACcag                                                                            <  2:806337/140‑1 (MQ=255)
                                                     tACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAGTGATTGACTCCACGAATCAGTGCCTTCTTGATCGTATCGGAGAGCTTAAATCGGGCGATGCTTGCATTGCAGAATACcag                                                                            <  2:828235/140‑1 (MQ=255)
                                                           aTGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAGTGATTGACTCCACGAATCAGTGCCTTCTTGATCGTATCGGAGAGCTTAAATCGGGCGATGCTTGCATTGCAGAATACCAGCAGGCt                                                                      >  1:1001071/1‑140 (MQ=255)
                                                                      atatTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAGTGATTGACTCCACGAATCAGTGCCTTCTTGATCGTATCGGAGAGCTTAAATCGGGCGATGCTTGCATTGCAGAATACCAGCAGGCTGGCCGTGGTCg                                                           >  1:563723/1‑140 (MQ=255)
                                                                      atatTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAGTGATTGACTCCACGAATCAGTGCCTTCTCGATCGTATCGGAGAGCTTAAATCGGGCGATGCTTGCATTGCAGAATACCAGCAGGCTGGCCGTGGTCg                                                           >  2:709392/1‑140 (MQ=255)
                                                                         ttGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAGTGATTGACTCCACGAATCAGTGCCTTCTTGATCGTATCGGAGAGCTTAAATCGGGCGATGCTTGCATTGCAGAATACCAGCAGGCTGGCCGTGGTCGCCg                                                        <  2:555842/140‑1 (MQ=255)
                                                                           gggTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAGTGATTGACTCCACGAATCAGTGCCTTCTTGATCGTATCGGAGAGCTTAAATCGGGCGATGCTTGCATTGCAGAATACCAGCAGGCTGGCCGTGGTCGCCggg                                                      <  1:772078/140‑1 (MQ=255)
                                                                           gggTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAGTGATTGACTCCACGAATCAGTGCCTTCTTGATCGTATCGGAGAGCTTAAATCGGGCGATGCTTGCATTGCAGAATACCAGCAGGCTGGCCGTGGTCGCCggg                                                      <  2:150791/140‑1 (MQ=255)
                                                                           gggTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAGTGATTGACTCCACGAATCAGTGCCTTCTTGATCGTATCGGAGAGCTTAAATCGGGCGATGCTTGCATTGCAGAATACCAGCAGGCTGGCCGTGGTCGCCggg                                                      <  1:596009/140‑1 (MQ=255)
                                                                             gTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAGTGATTGACTCCACGAATCAGTGCCTTCTTGATCGTATCGGAGAGCTTAAATCGGGCGATGCTTGCATTGCAGAATACCAGCAGGCTGGCCGTGGTCGCCGGGGt                                                    >  1:21971/1‑140 (MQ=255)
                                                                                aGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAGTGATTGACTCCACGAATCAGTGCCTTCTTGATCGTATCGGAGAGCTTAAATCGGGCGATGCTTGCATTGCAGAATACCAGCAGGCTGGCCGTGGTCGCCGGGGTCgg                                                 >  1:590053/1‑140 (MQ=255)
                                                                                                    gCCGTGCTGCCAGTGATTGACTCCACGAATCAGTGCCTTCTTGATCTTATCGGAGAGCTTAAATCGGGCGATGCTTGCATTGCAGAATACCAGCAGGCTGGCCGTGGTCGCCGGGGTCGGAAATGGTTTTCGCCTTTTgg                             >  1:410411/1‑140 (MQ=255)
                                                                                                      cGTGCTGCCAGTGATTGACTCCACGAATCAGTGCCTTCTTGATCGTATCGGAGAGCTTAAATCGGGCGATGCTTGCATTGCAGAATACCAGCAGGCTGGCCGTGGTCGCCGGGGTCGGAAATGGTTTTCGCCTTTTGgcg                           <  2:492445/140‑1 (MQ=255)
                                                                                                      cGTGCTGCCAGTGATTGACTCCACGAATCAGTGCCTTCTTGATCGTATCGGAGAGCTTAAATCGGGCGATGCTTGCATTGCAGAATACCAGCAGGCTGGCCGTGGTCGCCGGGGTCGGAAATGGTTTTCGCCTTTTGgcg                           <  2:1001071/140‑1 (MQ=255)
                                                                                                      cGTGCTGCCAGTGATTGACTCCACGAATCAGTGCCTTCTTGATCGTATCGGAGAGCTTAAATCGGGCGATGCTTGCATTGCAGAATACCAGCAGGCTGGCCGTGGTCGCCGGGGTCGGAAATGGTTTTCGCCTTTTGgcg                           <  2:934952/140‑1 (MQ=255)
                                                                                                       gTGCTGCCAGTGATTGACTCCACGAATCAGTGCCTTCTTGATCGTATCGGAGAGCTTAAATCGGGCGATGCTTGCATTGCAGAATACCAGCAGGCTGGCCGTGGTCGCCGGGGTCGGAAATGGTTTTCGCCTTTTGgcgc                          >  1:245089/1‑140 (MQ=255)
                                                                                                         gctgcCAGTGATTGACTCCACGAATCAGTGCCTTCTTGATCGTATCGGAGAGCTTAAATCGGGCGATGCTTGCATTGCAGAATACCAGCAGGCTGGCCGTGGTCGCCGGGGTCGGAAATGGTTTTCGCCTTTTGgcg                           <  2:985668/137‑1 (MQ=255)
                                                                                                         gctgcCAGTGATTGACTCCACGAATCAGTGCCTTCTTGATCGTATCGGAGAGCTTAAATCGGGCGATGCTTGCATTGCAGAATACCAGCAGGCTGGCCGTGGTCGCCGGGGTCGGAAATGGTTTTCGCCTTTTGgcg                           >  1:985668/1‑137 (MQ=255)
                                                                                                         gctgcCAGTGATTGACTCCACGAATCAGTGCCTTCTTGATCGTATCGGAGAGCTTAAATCGGGCGATGCTTGCATTGCAGAATACCAGCAGGCTGGCCGTGGTCGCCGGGGTCGGAAATGGTTTTCGCCTTTTGGCGCaa                        >  2:300905/1‑140 (MQ=255)
                                                                                                             ccAGTGATTGACTCCACGAATCAGTGCCTTCTTGATCGTATCGGAGAGCTTAAATCGGGCGATGCTTGCATTGCAGAATACCAGCAGGCTGGCCGTGGTCGCCGGGGTCGGAAATGGTTTTCGCCTTTTGGCGCAAACtt                    <  2:223205/140‑1 (MQ=255)
                                                                                                               aGTGATTGACTCCACGAATCAGTGCCTTCTTGATCGTATCGGAGAGCTTAAATCGGGCGATGCTTGCATTGCAGAATACCAGCAGGCTGGCCGTGGTCGCCGGGGTCGGAAATGGTTTTCGCCTTTTGGCGCAAACTtat                  <  1:787894/140‑1 (MQ=255)
                                                                                                                     tGACTCCACGAATCAGTGCCTTCTTGATCGTATCGGAGAGCTTAAATCGGGCGATGCTTGCATTGCAGAATACCAGCAGGCTGGCCGTGGTCGCCGGGGTCGGAAATGGTTTTCGCCTTTTGGCGCAAACTTATATTTGt            <  2:590053/140‑1 (MQ=255)
                                                                                                                           cACGAATCAGTGCCTTCTTGATCGTATCGGAGAGCTTAAATCGGGCGATGCTTGCATTGCAGAATACCAGCAGGCTGGACGTGGTCGCCGGGGTCGGAAATGGTTTTCGCCTTTTGGCGCAAACTTATATTTGTCGATGt      >  1:86777/1‑140 (MQ=255)
                                                                                                                               aaTCAGTGCCTTCTTGATCGTATCGGAGAGCTTAAATCGGGCGATGCTTGCATTGCAGAATACCAGCAGGCTGGCCGTGGTAGCCGGGGTCGGAAATGGTTTTCGCCTTTTGGCGCAAACTTATATTTGTCGATGTTCTg  <  1:160552/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                    
TGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAGTGATTGACTCCACGAATCAGTACCTTCTTGATCGTATCGGAGAGCTTAAATCGGGCGATGCTTGCATTGCAGAATACCAGCAGGCTGGCCGTGGTCGCCGGGGTCGGAAATGGTTTTCGCCTTTTGGCGCAAACTTATATTTGTCGATGTTCTG  >  NC_000913/4173225‑4173491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A