Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	155	60.3	2039550	99.3%	2025273	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,264,347	G→A	A12T (GCT→ACT)	dnaB →	replicative DNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,264,347	0	G	A	95.3%	137.0 / ‑1.6	43	A12T (GCT→ACT)	dnaB	replicative DNA helicase
Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (19/22); total (20/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

TCCAGAACGTATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATAACGAACGCTGGGATGATGTAGCCGAGCGTGTGGTA  >  NC_000913/4264238‑4264481

tccAGAACGTATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACAGCAGACTGAACCCCGCGAACGCGATCCACAAGTTg                                                                                                          >  2:700363/1‑140 (MQ=255)

 ccAGAACGTATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACAGCAGACTGAACCCCGCGAACGCGATCCACAAGTTGc                                                                                                         <  2:823083/140‑1 (MQ=255)

 ccAGAACGTATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACAGCAGACTGAACCCCGCGAACGCGATCCACAAGTTGc                                                                                                         <  1:700363/140‑1 (MQ=255)

     aaCGTATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACAGCAGACTGAACCCCGCGAACGCGATCCACAAGTTGCCggg                                                                                                     >  1:687837/1‑140 (MQ=255)

       cGTATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACAGCAGACTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCt                                                                                                   >  1:480373/1‑140 (MQ=255)

             gTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACAGCAGACTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGt                                                                                             <  2:480373/140‑1 (MQ=255)

                 gggTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACAGCAGACTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCt                                                                                         <  2:241867/140‑1 (MQ=255)

                 gggTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACAGCAGACTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCt                                                                                         >  1:778934/1‑140 (MQ=255)

                    tCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACAGCAGACTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCg                                                                                      <  2:861657/140‑1 (MQ=255)

                             tatGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACAGCAGACTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACtcgatc                                                                             >  1:548046/1‑140 (MQ=255)

                             tatGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACAGCAGACTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACtcgatc                                                                             >  2:175474/1‑140 (MQ=255)

                                  tAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACAGCAGACTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGc                                                                        >  1:601978/1‑140 (MQ=255)

                                      gTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACAGCAGACTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAg                                                                    <  2:815262/140‑1 (MQ=255)

                                          cGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACAGCAGACTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGt                                                                <  2:629191/140‑1 (MQ=255)

                                          cGACCCATTCTTTATCTCGCTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACAGCAGACTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGt                                                                <  2:23071/140‑1 (MQ=255)

                                            aCCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACAGCAGACTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCg                                                              >  2:392038/1‑140 (MQ=255)

                                                 ttCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGt                                                                <  2:502820/133‑1 (MQ=255)

                                                 ttCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGt                                                                >  1:502820/1‑133 (MQ=255)

                                                    tttATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACAGCAGACTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTggg                                                      <  1:392038/140‑1 (MQ=255)

                                                           cGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACAGCAGACTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTa                                               >  2:255629/1‑140 (MQ=255)

                                                                  tCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACAGCAGACTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGTAGCGGAGCAGTCGGTGTTGGGCGGTTTAATg                                            >  2:952819/1‑136 (MQ=255)

                                                                  tCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACAGCAGACTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATg                                            <  1:952819/136‑1 (MQ=255)

                                                                  tCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACAGCAGACGGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACGCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAg                                        >  2:701570/1‑140 (MQ=255)

                                                                       tCACTATGGCAGGAAATAAACCCTTCAACAAACAGCAGACTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATaac                                   <  2:292528/140‑1 (MQ=255)

                                                                          cTATGGCAGGAAATAAACCCTTCAACAAACAGCAGACTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATaacgaa                                <  2:937600/140‑1 (MQ=255)

                                                                            aTGGCAGGAAATAAACCCTTCAACAAACAGCAGACTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATaacgaacg                              <  1:987762/140‑1 (MQ=255)

                                                                              ggCAGGAAATAAACCCTTCAACAAACAGCAGACTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGa                                       <  2:75078/129‑1 (MQ=255)

                                                                              ggCAGGAAATAAACCCTTCAACAAACAGCAGACTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGa                                       >  1:75078/1‑129 (MQ=255)

                                                                              ggCAGGAAATAAACCCTTCAACAAACAGCAGACTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATAACGAACGCt                            >  1:639963/1‑140 (MQ=255)

                                                                                cAGGAAATAAACCCTTCAACAAACAGCAGACTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATAACGAACGCTgg                          <  1:195374/140‑1 (MQ=255)

                                                                                  ggAAATAAACCCTTCAACAAACAGCAGACTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATAACGAACGCTGGga                        <  1:175474/140‑1 (MQ=255)

                                                                                  ggAAATAAACCCTTCAACAAACAGCAGACTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATAACGAACGCTGGga                        <  2:853130/140‑1 (MQ=255)

                                                                                  ggAAATAAACCCTTCAACAAACAGCAGACTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATAACGAACGCTGGga                        <  1:846253/140‑1 (MQ=255)

                                                                                     aaTAAACCCTTCAACAAACAGCAGACTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATAACGAACGCTGGgatga                     >  2:180480/1‑140 (MQ=255)

                                                                                              ttCAACAAACAGCAGACTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATAACGAACGCTGGGATGATGTAGCCGa            >  2:443969/1‑140 (MQ=255)

                                                                                              ttCAACAAACAGCAGACTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATAACGAACGCTGGGATGATGTAGCCGa            <  1:443969/140‑1 (MQ=255)

                                                                                               tCAACAAACAGCAGACTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATAACGAACGCTGGGATGATGTAGCCGAg           <  2:778934/140‑1 (MQ=255)

                                                                                               tCAACAAACAGCAGACTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATAACGAACGCTGGGATGATGTAGCCGAg           <  1:973301/140‑1 (MQ=255)

                                                                                                caacaaACAGCAGACTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATAACGAACGCTGGga                        >  1:731075/1‑126 (MQ=255)

                                                                                                caacaaACAGCAGACTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATAACGAACGCTGGga                        <  2:731075/126‑1 (MQ=255)

                                                                                                caacaaACAGCAGACTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATAACGAACGCTGGGATGATGTAGCCGAGc          >  1:534877/1‑140 (MQ=255)

                                                                                                 aacaaacaGCAGACTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATAACGAACGCTGGGATGATGTAGCCGAGCg         >  1:733451/1‑140 (MQ=255)

                                                                                                        agcagACTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATAACGAACGCTGGGATGATGTAGCCGAGCGTGTGGTa  >  2:1003944/1‑140 (MQ=255)

TCCAGAACGTATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATAACGAACGCTGGGATGATGTAGCCGAGCGTGTGGTA  >  NC_000913/4264238‑4264481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A