Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F28 I1 R1	151	87.4	2971636	99.2%	2947862	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	30,564	T→C	V305A (GTA→GCA)	carA →	carbamoyl phosphate synthetase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	30,564	0	T	C	100.0%	186.7 / NA	53	V305A (GTA→GCA)	carA	carbamoyl phosphate synthetase subunit alpha
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (23/30); total (23/30)

GAAACCGATATTCCGGTATTCGGCATCTGTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGC  >  NC_000913/30428‑30697

gAAACCGATATTCCGGTATTCGGCATCTGTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGCAAt                                                                                                                                    <  2:1110290/140‑1 (MQ=255)

  aaCCGATATTCCGGTATTCGGCATCTGTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGCAATGa                                                                                                                                  >  2:316132/1‑140 (MQ=255)

     cGATATTCCGGTATTCGGCATCTGTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGCAATGATCa                                                                                                                               <  2:1339556/140‑1 (MQ=255)

               gTATTCGGCATCTGTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGCAATGATCACCGCCCAGaa                                                                                                                     >  1:398503/1‑140 (MQ=255)

                    cGGCATCTGTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGCAATGATCACCGCCCAGAACCa                                                                                                                  <  1:1072373/138‑1 (MQ=255)

                    cGGCATCTGTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGCAATGATCACCGCCCAGAACCa                                                                                                                  >  2:1072373/1‑138 (MQ=255)

                     ggCATCTGTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGCAATGATCACCGCCCAGAACCACgg                                                                                                               >  2:184907/1‑140 (MQ=255)

                     ggCATCTGTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGCAATGATCACCGCCCAGAACCACgg                                                                                                               >  2:266739/1‑140 (MQ=255)

                       cATCTGTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGCAATGATCACCGCCCAGAACCACGGtt                                                                                                             <  2:740924/140‑1 (MQ=255)

                          cTGTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGCAATGATCACCGCCCAGAACCACGGTTTTg                                                                                                          <  1:896073/140‑1 (MQ=255)

                          cTGTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGCAATGATCACCGCCCAGAACCACGGTTTTg                                                                                                          <  1:732630/140‑1 (MQ=255)

                            gTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGCAATGATCACCGCCCAGAACCACGGTTTTGCg                                                                                                        <  1:1361676/140‑1 (MQ=255)

                            gTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGCAATGATCACCGCCCAGAACCACGGTTTTGCg                                                                                                        >  2:1053147/1‑140 (MQ=255)

                                 ggTCATCAGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGCAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGa                                                                                                   <  2:1346490/140‑1 (MQ=255)

                                 ggTCATCAGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGCAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGa                                                                                                   <  1:1207838/140‑1 (MQ=255)

                                   tcatcaGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGCAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACg                                                                                                 <  1:1322987/140‑1 (MQ=255)

                                    catcaGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGCAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGa                                                                                                <  1:1110051/140‑1 (MQ=255)

                                     atcaGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGCAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGaa                                                                                               <  1:184907/140‑1 (MQ=255)

                                     atcaGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGCAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGaa                                                                                               >  2:645938/1‑140 (MQ=255)

                                            gctggcgctggcGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGCAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACAt                                                                                        >  1:948254/1‑140 (MQ=255)

                                             ctggcgctggcgAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGCAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACAtt                                                                                       <  1:711791/140‑1 (MQ=255)

                                                     ggcgAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGCAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCaa                                                                               >  1:587295/1‑140 (MQ=255)

                                                          gCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGCAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCaaa                                                                              >  1:1446187/1‑136 (MQ=255)

                                                          gCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGCAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCaaa                                                                              <  2:1446187/136‑1 (MQ=255)

                                                              tGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGCAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCgtg                                                                      <  1:89603/140‑1 (MQ=255)

                                                              tGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGCAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCgtg                                                                      >  2:591377/1‑140 (MQ=255)

                                                               gCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGCAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCgtgt                                                                     <  1:716313/140‑1 (MQ=255)

                                                                 gAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGCAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCa                                                                   <  2:1103507/140‑1 (MQ=255)

                                                                 gAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGCAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCa                                                                   <  2:587295/140‑1 (MQ=255)

                                                                    gACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGCAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGc                                                                <  2:539277/140‑1 (MQ=255)

                                                                       tGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGCAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGCATa                                                             <  1:645938/140‑1 (MQ=255)

                                                                       tGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGCAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGCATa                                                             <  1:266739/140‑1 (MQ=255)

                                                                        gTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGCAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGCAt                                                              <  1:77387/138‑1 (MQ=255)

                                                                        gTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGCAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGCAt                                                              >  2:77387/1‑138 (MQ=255)

                                                                        gTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGCAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGCATaa                                                            >  1:1469361/1‑140 (MQ=255)

                                                                               tGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGCAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGCATAAATCCCTg                                                     <  1:316132/140‑1 (MQ=255)

                                                                                    tttGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGCAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGa                                                <  1:926173/140‑1 (MQ=255)

                                                                                        gTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGCAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGt                                            <  1:648470/140‑1 (MQ=255)

                                                                                             cacGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGCAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGtt                                       <  1:742732/140‑1 (MQ=255)

                                                                                                  cggcAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGCAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTAc                                     <  2:478383/137‑1 (MQ=255)

                                                                                                  cggcAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGCAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTAc                                     >  1:478383/1‑137 (MQ=255)

                                                                                                  cggcAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGCAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAgg                                  >  2:394254/1‑140 (MQ=255)

                                                                                                    gcAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGCAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGc                                <  2:398503/140‑1 (MQ=255)

                                                                                                     cAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGCAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCa                               >  2:1242576/1‑140 (MQ=255)

                                                                                                          aTCCGGTTAAAGATGTGGAGAAAAACGTGGCAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCAt                          >  2:491916/1‑140 (MQ=255)

                                                                                                            ccGGTTAAAGATGTGGAGAAAAACGTGGCAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCg                        >  1:55115/1‑140 (MQ=255)

                                                                                                              ggTTAAAGATGTGGAGAAAAACGTGGCAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCa                      <  1:491916/140‑1 (MQ=255)

                                                                                                              ggTTAAAGATGTGGAGAAAAACGTGGCAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCa                      <  2:948254/140‑1 (MQ=255)

                                                                                                                         tgGAGAAAAACGTGGCAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCg           >  1:865259/1‑140 (MQ=255)

                                                                                                                         tgGAGAAAAACGTGGCAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCg           >  2:885123/1‑140 (MQ=255)

                                                                                                                                aaaCGTGGCAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCa    >  2:488779/1‑140 (MQ=255)

                                                                                                                                  aCGTGGCAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGc  >  2:534630/1‑140 (MQ=255)

                                                                                                                                  aCGTGGCAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGc  >  1:33187/1‑140 (MQ=255)

GAAACCGATATTCCGGTATTCGGCATCTGTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGC  >  NC_000913/30428‑30697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A