Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F28 I1 R1	151	87.4	2971636	99.2%	2947862	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	160,139	A→G	intergenic (‑27/+10)	gluQ ← / ← dksA	glutamyl‑Q tRNA(Asp) synthetase/RNA polymerase‑binding transcription factor DksA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	160,139	0	A	G	100.0%	173.6 / NA	53	intergenic (‑27/+10)	gluQ/dksA	glutamyl‑Q tRNA(Asp) synthetase/RNA polymerase‑binding transcription factor DksA
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (31/22); total (31/22)

AGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAACGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGCAGTAGCCGA  >  NC_000913/160019‑160273

agCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAGCGGCTGTAATTagccagcc                                                                                                                     <  1:541075/140‑1 (MQ=255)

         gAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAGCGGCTGTAATTAGCCAGCCATCTGtttt                                                                                                            >  1:1431349/1‑140 (MQ=255)

                     aaGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAAAGTGATGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTca                                                                                                >  1:727779/1‑140 (MQ=255)

                               ggCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCAGGAGCATTTCCCGCCTGTGGTAAACGTGGTGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGttt                                                                                      >  2:543161/1‑140 (MQ=255)

                                 cGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCTTTTCCCGCCTGTGGTAAACGTGATGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTg                                                                                    <  2:1344293/140‑1 (MQ=255)

                                 cGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTg                                                                                    >  1:1004945/1‑140 (MQ=255)

                                     gCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGt                                                                                <  1:190505/140‑1 (MQ=255)

                                     gCGGCAAATATACTGTGTGTTTATCATCTNTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAGCGGCTGTAATTAGCCNGCCATCTGTTTTNCGCGAATTTCAGCCAGCGTTNNGCAGt                                                                                <  2:910772/140‑1 (MQ=255)

                                       ggCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCCCCTGTGGTAAACGTGATGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTTCAGTCg                                                                              >  2:290013/1‑140 (MQ=255)

                                            atatACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCTTTTCCCGCCTGTGGTAAACGTGATGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGca                                                                         <  2:1431349/140‑1 (MQ=255)

                                                   gtgtgtCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGAt                                                                    >  2:59916/1‑138 (MQ=255)

                                                   gtgtgtCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGAt                                                                    <  1:59916/138‑1 (MQ=255)

                                                      tgtctgtcATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCt                                                               >  2:1193252/1‑140 (MQ=255)

                                                      tgtctgtcATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCt                                                               >  1:994933/1‑140 (MQ=255)

                                                      tgtctgtcATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCt                                                               >  1:804120/1‑140 (MQ=255)

                                                       gtctgtcATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTg                                                              >  2:1387165/1‑140 (MQ=255)

                                                       gtctgtcATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTg                                                              >  1:1199840/1‑140 (MQ=255)

                                                        tctgtcATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGt                                                             >  2:1360834/1‑140 (MQ=255)

                                                        tctgtcATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGt                                                             >  2:1014670/1‑140 (MQ=255)

                                                         ctgtcATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTc                                                            >  1:1013850/1‑140 (MQ=255)

                                                           gtcATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCgg                                                          >  1:1245653/1‑140 (MQ=255)

                                                              ttcttTTTGAAAAAAAAATAAGGGGGGAGCTTTCCCCGCCTGTGGTAAACGTGATGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGgcg                                                       <  2:727779/139‑1 (MQ=255)

                                                              aTCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTTTCGAATTTAAGCCAGCGTTTTGCAGCCGATGCACAGATCGGCTGCCGGgcg                                                       >  1:177823/1‑140 (MQ=255)

                                                               tctcTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGgcgc                                                      >  1:335512/1‑140 (MQ=255)

                                                                ctttttGAACAAAAAATAAGGTGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGgcgcg                                                     <  2:10715/137‑1 (MQ=255)

                                                                ctctTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGgcgcg                                                     <  1:1387165/140‑1 (MQ=255)

                                                                   tttGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCtt                                                  >  2:1397070/1‑140 (MQ=255)

                                                                       aaCAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAg                                              <  2:994933/140‑1 (MQ=255)

                                                                         aaaaaaaTAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAGCGGCTGTAATTAGCCAGCTATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGAc                                            <  2:1161077/139‑1 (MQ=255)

                                                                             aaaTAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTTGCGAATTTCAACCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCg                                        >  2:967795/1‑140 (MQ=255)

                                                                                   ggCGGGAGCTTTTCCCGCCTGTGGTAAACGTGATGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATAcc                                  <  2:259039/140‑1 (MQ=255)

                                                                                   ggCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATAcc                                  <  1:1397070/140‑1 (MQ=255)

                                                                                    gCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCGGTCGGGCGCGCTTCCAGACGGCGAATAGCa                                 >  2:914022/1‑140 (MQ=255)

                                                                                        gAGCATTTCCCGCCTGTGGTAAACGTGATGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAAttt                             >  1:665130/1‑140 (MQ=255)

                                                                                        gAGCATTTCCCGCCTGTGGTAAACGTGATGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAAttt                             >  2:692614/1‑140 (MQ=255)

                                                                                          gCATTTCCCGCCTGTGGTAAACGTGATGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCa                           <  2:804120/140‑1 (MQ=255)

                                                                                           cATTTCCCGCCTGTGGTAAACGTGATGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCaa                          <  1:688598/140‑1 (MQ=255)

                                                                                           cATTTCCCGCCTGTGGTAAACGTGATGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCaa                          <  1:692614/140‑1 (MQ=255)

                                                                                                 ccGCCTGTGGTAAACGTGATGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGc                    <  2:1004945/140‑1 (MQ=255)

                                                                                                  cGCCTGTGGTAAACGTGGTGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCa                   <  1:543161/140‑1 (MQ=255)

                                                                                                  cGCCTGTGGTAAACGTGATGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCg                     <  2:981007/138‑1 (MQ=255)

                                                                                                  cGCCTGTGGTAAACGTGATGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCg                     >  1:981007/1‑138 (MQ=255)

                                                                                                  cGCCTGTGGTAAACGTGATGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCa                   >  1:1401911/1‑140 (MQ=255)

                                                                                                   gCCTGTGGTAAACGTGATGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCAg                  >  1:1371425/1‑140 (MQ=255)

                                                                                                       gtgGTAAACGTGATGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCAGGAtt              <  2:1199840/140‑1 (MQ=255)

                                                                                                        tgGTAAACGTGGTGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTc             >  1:884599/1‑140 (MQ=255)

                                                                                                        tgGTAAACGTGATGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTc             >  1:103495/1‑140 (MQ=255)

                                                                                                        tgGCAAACGTGATGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGGCGATGCACAGATTGGCTGTCTGGTTCGCTTCCAGACGTAGAAAACCAACTTCAATACCCAAGGAGTc             >  2:322266/1‑140 (MQ=255)

                                                                                                           tAAACGTGATGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGCa          <  1:149321/140‑1 (MQ=255)

                                                                                                             aaCGTGATGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGCAGt        <  2:665130/140‑1 (MQ=255)

                                                                                                                  gATGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGCAg         <  2:588115/134‑1 (MQ=255)

                                                                                                                  gATGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGCAg         >  1:588115/1‑134 (MQ=255)

                                                                                                                  gATGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGCAGTAGCCg   >  1:1456077/1‑140 (MQ=255)

                                                                                                                   aTGGAGCGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGCAGTAGCCGa  >  1:658739/1‑140 (MQ=255)

AGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAACGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGCAGTAGCCGA  >  NC_000913/160019‑160273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A