Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	159	82.1	2830106	99.3%	2810295	139.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	160,637	+C	intergenic (‑33/+145)	dksA ← / ← sfsA	RNA polymerase‑binding transcription factor DksA/putative DNA‑binding transcriptional regulator of maltose metabolism

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	160,633	1	.	C	98.1%	395.2 / ‑0.7	106	intergenic (‑29/+149)	dksA/sfsA	RNA polymerase‑binding transcription factor DksA/putative DNA‑binding transcriptional regulator of maltose metabolism
Reads supporting (aligned to +/- strand): ref base . (1/1); new base C (48/56); total (49/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 112 total aligned reads displayed.

CAGCTGGGCTTCATTCATATACTCTTCGCCCGGCTTCTCCTGATATGGTTCCACCCCAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGAT‑CCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAAT  >  NC_000913/160494‑160762

cAGCTGGGCTTCATTCATATACTCTTCGCCCGGCTTCTCCTGATATGGTTCCACCCCAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGAt                                                                                                                                    <  2:1092289/140‑1 (MQ=255)

           cattcatATACTCTTCGCCCAGCTTCTCCTGATATGGTTCCACCCCAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACCCGCACTATCGATCCCCCATGTTc                                                                                                                         <  2:676393/140‑1 (MQ=255)

                     ctctTCGCCCGGCTTCTCCTGATATGGTTCCACCCCAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGaaaaa                                                                                                               >  1:1159561/1‑140 (MQ=255)

                       ctTCGCCCGGCTTCTCCTGATATGGTTCCACCCCAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATg                                                                                                             >  2:842352/1‑140 (MQ=255)

                       ctTCGCCCGGCTTCTCCTGATATGGTTCCACCCCAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATg                                                                                                             >  2:342769/1‑140 (MQ=255)

                        ttCGCCCGGCTTCTCCTGATATGGTTCCACCCCAGCGATGGCGAGTATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGa                                                                                                            >  1:449965/1‑140 (MQ=255)

                        ttCGCCCGGCTTCTCCTGATATGGTTCCACCCCAGCGATGGCGAGAATACTCAGGTACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCggggg                                                                                                                    >  2:1262504/1‑132 (MQ=255)

                        ttCGCCCGGCTTCTCCTGATATGGTTCCACCCCAGCGATGGCGAGAATACTCAGGTACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCggggg                                                                                                                    <  1:1262504/132‑1 (MQ=255)

                        ttCGCCCGGCTTCTCCTGATATGGTTCCACCCCAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGa                                                                                                            >  2:649251/1‑140 (MQ=255)

                        ttCGCCCGGCTTCTCCTGATATGGTTCCACCCCAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGa                                                                                                            >  2:1321462/1‑140 (MQ=255)

                        ttCGCCCGGCTTCTCCTGATATGGTTCCACCCCAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGa                                                                                                            >  1:966137/1‑140 (MQ=255)

                        ttCGCCCGGCTTCTCCTGATATGGTTCCACCCCAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGa                                                                                                            >  2:476256/1‑140 (MQ=255)

                        ttCGCCCGGCTTCTCCTGATATGGTTCCACCCCAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGa                                                                                                            >  2:1161752/1‑140 (MQ=255)

                        ttCGCCCGGCTTCTCCTGATATGGTTCCACCCCAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGAT‑CCCCATGTTCGGGGGAAAAATGAg                                                                                                           >  1:959726/1‑140 (MQ=255)

                          cGCCCGGCTTCTCCTGATATGGTTCCACCCCAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAgg                                                                                                          >  2:388767/1‑140 (MQ=255)

                          cGCCCGGCTTCTCCTGATATGGTTCCACCCCAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAgg                                                                                                          <  2:483110/140‑1 (MQ=255)

                          cGCCCGGCTTCTCCTGATATGGTTCCACCCCAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAgg                                                                                                          <  2:522799/140‑1 (MQ=255)

                          cGCCCGGCTTCTCCTGATATGGTTCCACCCCAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAgg                                                                                                          >  1:536828/1‑140 (MQ=255)

                          cGCCCGGCTTCTCCTGATATGGTTCCACCCCAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAgg                                                                                                          <  2:292651/140‑1 (MQ=255)

                          cGCCCGGCTTCTCCTGATATGGTTCCACCCCAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAgg                                                                                                          <  2:190965/140‑1 (MQ=255)

                          cGCCCGGCTTCTCCTGATATGGTTCCACCCCAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAgg                                                                                                          >  1:320359/1‑140 (MQ=255)

                          cGCCCGGCTTCTCCTGATATGGTTCCACCCCAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAgg                                                                                                          <  1:768533/140‑1 (MQ=255)

                          cGCCCGGCTTCTCCTGATATGGTTCCACCCCAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAgg                                                                                                          >  1:292651/1‑140 (MQ=255)

                          cGCCCGGCTTCTCCTGATATGGTTCCACCCCAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAgg                                                                                                          <  1:147209/140‑1 (MQ=255)

                          cGCCCGGCTTCTCCTGATATGGTTCCACCCCAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAgg                                                                                                          >  2:1136716/1‑140 (MQ=255)

                          cGCCCGGCTTCTCCTGATATGGTTCCACCCCAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAgg                                                                                                          <  1:1161752/140‑1 (MQ=255)

                          cGCCCGGCTTCTCCTGATATGGTTCCACCCCAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAgg                                                                                                          <  2:928314/140‑1 (MQ=255)

                          cGCCCGGCTTATCCTGATATTTTTCCACCCCAGCGATGGCGAGAATGCTCAGGGACGATGTTTTTCGGTTTTGGCCTTCTTGCGTGTTGCTTCTCCTTAGCACGTACTATCGATCCCCCATGTTCGGGGGAAAAATGAgg                                                                                                          <  2:166281/140‑1 (MQ=255)

                             ccGGCTTCTCCTGATATGGTTCCACCCCAGCGATGGCGAGAATACTCAGGGATGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAgg                                                                                                          >  2:1254876/1‑137 (MQ=255)

                             ccGGCTTCTCCTGATATGGTTCCACCCCAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCg                                                                                                       <  2:1159561/140‑1 (MQ=255)

                             ccGGCTTCTCCTGATATGGTTCCACCCCAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCg                                                                                                       <  1:1402320/140‑1 (MQ=255)

                             ccGGCTTCTCCTGATATGGTTCCACCCCAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCg                                                                                                       <  2:864238/140‑1 (MQ=255)

                               ggCTTCTCCTGATATGGTTCCACCCCAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCt                                                                                                     <  1:1260133/140‑1 (MQ=255)

                               ggCTTCTCCTGATATGGTTCCACCCCAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCt                                                                                                     <  1:468214/140‑1 (MQ=255)

                                gCTTCTCCTGATATGGTTCCACCCCAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAgg                                                                                                          <  2:1115988/134‑1 (MQ=255)

                                gCTTCTCCTGATATGGTTCCACCCCAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAgg                                                                                                          >  1:1115988/1‑134 (MQ=255)

                                gCTTCTCCTGATATGGTTCCACCCCAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCta                                                                                                    >  2:1389358/1‑140 (MQ=255)

                                    ctcCTGATATGGTTCCACCCCAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATaaa                                                                                                <  1:79312/140‑1 (MQ=255)

                                               gTTCCACCCCAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCt                                                                                     >  2:914529/1‑140 (MQ=255)

                                                ttCCACCCCAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCtt                                                                                    >  1:413384/1‑140 (MQ=255)

                                                ttCCACCCCAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCtt                                                                                    >  2:85565/1‑140 (MQ=255)

                                                 tCCACCCCAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATg                                                                                       <  2:24238/136‑1 (MQ=255)

                                                 tCCACCCCAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATg                                                                                       >  1:24238/1‑136 (MQ=255)

                                                   cACCCCAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTc                                                                                 >  1:125638/1‑140 (MQ=255)

                                                    aCCCCAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTcc                                                                                >  2:544651/1‑140 (MQ=255)

                                                    aCCCCAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTcc                                                                                >  1:266593/1‑140 (MQ=255)

                                                     ccccAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCg                                                                               <  1:1136716/140‑1 (MQ=255)

                                                     ccccAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCg                                                                               >  1:9483/1‑140 (MQ=255)

                                                     ccccAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCg                                                                               >  1:1410341/1‑140 (MQ=255)

                                                       ccAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGa                                                                             <  2:536828/140‑1 (MQ=255)

                                                          gCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAg                                                                          >  1:334855/1‑140 (MQ=255)

                                                              tGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAAt                                                                      <  2:1401064/140‑1 (MQ=255)

                                                                 cGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTAt                                                                   <  2:9483/140‑1 (MQ=255)

                                                                 cGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTAt                                                                   <  2:1410341/140‑1 (MQ=255)

                                                                  gagaATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATc                                                                  >  2:266527/1‑140 (MQ=255)

                                                                     aaTACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTaa                                                               >  1:713389/1‑140 (MQ=255)

                                                                           cAGGGACGATGTTTTTCGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTaa                                                         <  2:266593/140‑1 (MQ=255)

                                                                           cAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTTGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTaa                                                         <  2:966137/140‑1 (MQ=255)

                                                                           cAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTaa                                                         <  2:1082223/140‑1 (MQ=255)

                                                                           cAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTaa                                                         <  1:388767/140‑1 (MQ=255)

                                                                           cAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTaa                                                         <  2:1113727/140‑1 (MQ=255)

                                                                           cAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTaa                                                         <  1:1321462/140‑1 (MQ=255)

                                                                           cAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTaa                                                         <  1:476256/140‑1 (MQ=255)

                                                                           cAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTaa                                                         <  1:842352/140‑1 (MQ=255)

                                                                             gggACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAaca                                                       <  1:544651/140‑1 (MQ=255)

                                                                              ggACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAacac                                                      >  1:952175/1‑140 (MQ=255)

                                                                              ggACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAacac                                                      >  1:308831/1‑140 (MQ=255)

                                                                              ggACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAacac                                                      >  1:562998/1‑140 (MQ=255)

                                                                                 cGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCt                                                                 <  1:120500/126‑1 (MQ=255)

                                                                                 cGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCt                                                                 >  2:120500/1‑126 (MQ=255)

                                                                                 cGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTg                                                   >  2:621588/1‑140 (MQ=255)

                                                                                 cGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTg                                                   <  1:321119/140‑1 (MQ=255)

                                                                                    tGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGAT‑CCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACaa                                               <  2:959726/140‑1 (MQ=255)

                                                                                     gTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACaa                                               <  1:342769/140‑1 (MQ=255)

                                                                                     gTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACaa                                               <  2:334855/140‑1 (MQ=255)

                                                                                     gTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACaa                                               <  1:914529/140‑1 (MQ=255)

                                                                                         tACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAAc                                              >  2:1015871/1‑137 (MQ=255)

                                                                                         tACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAAc                                              <  1:1015871/137‑1 (MQ=255)

                                                                                           cGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACtgtgt                                         <  1:649251/140‑1 (MQ=255)

                                                                                              ttttGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACaa                                               <  1:329393/131‑1 (MQ=255)

                                                                                              ttttGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACCATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACaa                                               >  2:329393/1‑131 (MQ=255)

                                                                                                ttGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACaa                                               <  2:45327/129‑1 (MQ=255)

                                                                                                ttGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACaa                                               >  1:45327/1‑129 (MQ=255)

                                                                                                ttGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACa                                                >  2:165015/1‑128 (MQ=255)

                                                                                                ttGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACa                                                <  1:165015/128‑1 (MQ=255)

                                                                                                ttGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGa                                    >  2:241834/1‑140 (MQ=255)

                                                                                                ttGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGa                                    >  2:777371/1‑140 (MQ=255)

                                                                                                 tGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGaa                                   <  1:558851/140‑1 (MQ=255)

                                                                                                    ccTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAg                                <  2:308831/140‑1 (MQ=255)

                                                                                                    ccTTCTTACATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAg                                <  1:1241892/140‑1 (MQ=255)

                                                                                                        cttGCATGTTGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTa                            >  1:1012748/1‑140 (MQ=255)

                                                                                                                ttGCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAGAAGCGTATTTGCGCa                    <  2:413384/140‑1 (MQ=255)

                                                                                                                  gCTTCTCCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCAcg                  <  1:266527/140‑1 (MQ=255)

                                                                                                                      ctcCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGAc              <  1:85565/140‑1 (MQ=255)

                                                                                                                       tcCTTAACACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAACAATTATCTAAACGTAACACTTGACAACTGTTTGAGGAAAAGCGTATTTGCGCACGCGAcc             <  2:449965/140‑1 (MQ=255)

                                                                                                                            aaCACGCACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTAT‑AATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATCTGCGCACGCGACCAGAATg       >  1:297677/1‑140 (MQ=255)

                                                                                                                                  cACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAAt  >  2:773092/1‑140 (MQ=255)

                                                                                                                                  cACTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAAt  >  2:257955/1‑140 (MQ=255)

                                                                                                                                   aCTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGaa         >  1:1141959/1‑132 (MQ=255)

                                                                                                                                   aCTATCGATCCCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGaa         <  2:1141959/132‑1 (MQ=255)

CAGCTGGGCTTCATTCATATACTCTTCGCCCGGCTTCTCCTGATATGGTTCCACCCCAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTGCCCTTCTTGCATGTTGCTTCTCCTTAACACGCACTATCGAT‑CCCCATGTTCGGGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAAT  >  NC_000913/160494‑160762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A