Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	260	73.5	2514320	99.3%	2496719	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	166,936	C→T	A736V (GCC→GTC)	mrcB →	peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	166,936	0	C	T	100.0%	259.2 / NA	74	A736V (GCC→GTC)	mrcB	peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcB
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (37/37); total (37/37)

CCGAACCTGCATCTGGCAGGGAAAACAGGGACTACCAACAATAACGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCG  >  NC_000913/166800‑167071

ccGAACCTGCATCTGGCAGGGAAAACAGGGACTACCAACAATAACGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAg                                                                                                                                      <  1:387443/140‑1 (MQ=255)

    aCCTGCATCTGGCAGGGAAAACAGGGACTACCAACAATAACGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCggg                                                                                                                                  >  1:209148/1‑140 (MQ=255)

    aCCTGCATCTGGCAGGGAAAACAGGGACTACCAACAATAACGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCggg                                                                                                                                  <  1:1221008/140‑1 (MQ=255)

     ccTGCATCTGGCAGGGAAAACAGGGACTACCAACAATAACGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCgggg                                                                                                                                 >  1:510074/1‑140 (MQ=255)

     ccTGCATCTGGCAGGGAAAACAGGGACTACCAACAATAACGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCgggg                                                                                                                                 >  1:143887/1‑140 (MQ=255)

     ccTGCATCTGGCAGGGAAAACAGGGACTACCAACAATAACGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCgggg                                                                                                                                 >  2:937888/1‑140 (MQ=255)

     ccTGCATCTGGCAGGGAAAACAGGGACTACCAACAATAACGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCgggg                                                                                                                                 <  2:209148/140‑1 (MQ=255)

      cTGCATCTGGCAGGGAAAACAGGGACTACCAACAATAACGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGc                                                                                                                                <  2:390627/140‑1 (MQ=255)

       tGCATCTGGCAGGGAAAACAGGGACTACCAACAATAACGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCa                                                                                                                               <  2:1077919/140‑1 (MQ=255)

         cATCTGGCAGGGAAAACAGGGACTACCAACAATAACGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAAt                                                                                                                             <  2:66521/140‑1 (MQ=255)

         cATCTGGCAGGGAAAACAGGGACTACCAACAATAACGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAAt                                                                                                                             >  2:570861/1‑140 (MQ=255)

              ggCAGGGAAAACAGGGACTACCAACAATAACGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGa                                                                                                                        >  2:263023/1‑140 (MQ=255)

              ggCAGGGAAAACAGGGACTACCAACAATAACGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGa                                                                                                                        >  1:172242/1‑140 (MQ=255)

                cAGGGAAAACAGGGACTACCAACAATAACGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGAtt                                                                                                                      >  2:849304/1‑140 (MQ=255)

                  gggAAAACAGGGACTACCAACAATAACGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTa                                                                                                                    <  1:849304/140‑1 (MQ=255)

                   ggAAAACAGGGACTACCAACAATAACGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTAt                                                                                                                   >  1:260896/1‑140 (MQ=255)

                       aaCAGGGACTACCAACAATAACGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGc                                                                                                               <  2:557208/140‑1 (MQ=255)

                           gggACTACCAACAATAACGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTa                                                                                                           <  1:23654/140‑1 (MQ=255)

                           gggACTACCAACAATAACGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTa                                                                                                           <  1:62424/140‑1 (MQ=255)

                           gggACTACCAACAATAACGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTa                                                                                                           <  1:263023/140‑1 (MQ=255)

                           gggACTACCAACAATAACGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTa                                                                                                           <  2:980818/140‑1 (MQ=255)

                            ggACTACCAACAATAACGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTAt                                                                                                          >  1:717285/1‑140 (MQ=255)

                            ggACTACCAACAATAACGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTAt                                                                                                          <  2:712395/140‑1 (MQ=255)

                             gACTACCAACAATAACGTAGATACCTGGCTTGCGGGCATTGCCGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTATc                                                                                                         >  2:330976/1‑140 (MQ=255)

                               cTACCAACAATAACGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTAGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTATCTg                                                                                                       >  2:1195411/1‑140 (MQ=255)

                                tACCAACAATAACGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTATCTgg                                                                                                      <  1:281848/140‑1 (MQ=255)

                                    aacaaTAACGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTaa                                                                                                  >  1:1026179/1‑140 (MQ=255)

                                       aaTAACGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGtt                                                                                                            <  2:1238277/127‑1 (MQ=255)

                                       aaTAACGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGtt                                                                                                            >  1:1238277/1‑127 (MQ=255)

                                            cGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGc                                                                                          >  1:1165373/1‑140 (MQ=255)

                                                 aTACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAAcg                                                                                     >  1:1011385/1‑140 (MQ=255)

                                                 aTACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAAcg                                                                                     <  2:260896/140‑1 (MQ=255)

                                                 aTACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAAcg                                                                                     >  1:934374/1‑140 (MQ=255)

                                                     cTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAATTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAAcg                                                                                     >  1:260078/1‑136 (MQ=255)

                                                     cTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAATTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAAcg                                                                                     <  2:260078/136‑1 (MQ=255)

                                                     cTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAAcgccgc                                                                                 >  1:325751/1‑140 (MQ=255)

                                                      tGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCt                                                                                >  2:772464/1‑140 (MQ=255)

                                                      tGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCt                                                                                <  2:934374/140‑1 (MQ=255)

                                                       ggTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTg                                                                               >  1:272828/1‑140 (MQ=255)

                                                              gggCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTg                                                                        >  1:829329/1‑140 (MQ=255)

                                                                 cATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTc                                                                     <  2:1011385/140‑1 (MQ=255)

                                                                 cATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTc                                                                     <  1:118756/140‑1 (MQ=255)

                                                                 cATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTc                                                                     <  1:772464/140‑1 (MQ=255)

                                                                           agcaCGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCagaaga                                                           <  2:325751/140‑1 (MQ=255)

                                                                           agcaCGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCagaaga                                                           <  2:829329/140‑1 (MQ=255)

                                                                           agcaCGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCagaaga                                                           <  2:494970/140‑1 (MQ=255)

                                                                           agcaCGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCagaaga                                                           >  2:194967/1‑140 (MQ=255)

                                                                           agcaCGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCagaaga                                                           <  1:1024846/140‑1 (MQ=255)

                                                                           agcaCGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCagaaga                                                           >  1:237921/1‑140 (MQ=255)

                                                                             caCGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCaga                                                              >  1:423785/1‑135 (MQ=255)

                                                                             caCGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCaga                                                              <  2:423785/135‑1 (MQ=255)

                                                                               cGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACTAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATAtt                                                       <  1:131324/140‑1 (MQ=255)

                                                                                 gTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGc                                                     <  2:1165373/140‑1 (MQ=255)

                                                                                           ccTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGc                                           <  1:570861/140‑1 (MQ=255)

                                                                                            cTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCg                                          >  1:221003/1‑140 (MQ=255)

                                                                                            cTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCg                                          <  2:272828/140‑1 (MQ=255)

                                                                                              gggTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTg                                        >  1:1235924/1‑140 (MQ=255)

                                                                                                gTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGa                                      >  1:1082465/1‑140 (MQ=255)

                                                                                                   ggCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTa                                   >  1:862454/1‑140 (MQ=255)

                                                                                                   ggCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTa                                   >  2:34401/1‑140 (MQ=255)

                                                                                                   ggCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTa                                   >  1:880534/1‑140 (MQ=255)

                                                                                                   ggCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTa                                   >  1:324316/1‑140 (MQ=255)

                                                                                                    gCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTac                                  >  2:740735/1‑140 (MQ=255)

                                                                                                    gCCGTGATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTac                                  >  2:169978/1‑140 (MQ=255)

                                                                                                         gATAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACgg                             <  1:26079/140‑1 (MQ=255)

                                                                                                          aTAACAACCAGCCGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGc                            >  1:446638/1‑140 (MQ=255)

                                                                                                                     ccGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACtttgtttg                 <  2:1026179/140‑1 (MQ=255)

                                                                                                                      cGACCAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCATGGACTACGACGGCAACTTTGTTTGc                >  1:877390/1‑140 (MQ=255)

                                                                                                                          cAAACTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCg            <  2:1107765/140‑1 (MQ=255)

                                                                                                                            aaCTGTATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGt          <  2:1235924/140‑1 (MQ=255)

                                                                                                                                 tATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCAt     >  1:699883/1‑140 (MQ=255)

                                                                                                                                 tATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCAt     <  1:647550/140‑1 (MQ=255)

                                                                                                                                 tATGGTGTCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCAt     <  2:172242/140‑1 (MQ=255)

                                                                                                                                    ggTGTCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCg  <  2:219284/140‑1 (MQ=255)

CCGAACCTGCATCTGGCAGGGAAAACAGGGACTACCAACAATAACGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCG  >  NC_000913/166800‑167071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A