Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F28 I1 R1	151	87.4	2971636	99.2%	2947862	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,864,197	G→A	A116A (GCG→GCA)	yeaD →	putative aldose 1‑epimerase YeaD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,864,197	0	G	A	100.0%	171.7 / NA	50	A116A (GCG→GCA)	yeaD	putative aldose 1‑epimerase YeaD
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (27/23); total (27/23)

GCTATTCGCGGTGGCGTACCGGTTTGCTGGCCGTGGTTTGGTCCGGCGGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTG  >  NC_000913/1864063‑1864333

gCTATTCGCGGTGGCGTACCGGTTTGCTGGCCGTGGTTTGGTCCGGCGGCACAACAAGGTCTGCCTGGGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCACTGAc                                                                                                                                     >  2:1442867/1‑140 (MQ=255)

         ggTGGCGTACCGGTTTGCTGGCCGTGGTTTGGTCCGGCGGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCACTGACTTTTGAAtt                                                                                                                            >  1:897793/1‑140 (MQ=255)

            ggCGTACCGGTTTGCTGGCCGTGGTTTGGTCCGGCGGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCACTGACTTTTGAATTGAc                                                                                                                         <  1:657992/140‑1 (MQ=255)

              cGTACCGGTTTGCTGGCCGTGGTTTGGTCCGGCGGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCACTGACTTTTGAATTGACGc                                                                                                                       >  1:111037/1‑140 (MQ=255)

              cGTACCGGTTTGCTGGCCGTGGTTTGGTCCGGCGGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCACTGACTTTTGAATTGACGc                                                                                                                       >  2:1156284/1‑140 (MQ=255)

               gTACCGGTTTGCTGGCCGTGGTTTGGTCCGGCGGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCACTGACTTTTGAATTGACGCa                                                                                                                      <  2:1284791/140‑1 (MQ=255)

                       ttGCTGGCCGTGGTTTGGTCCGGCGGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCACTGACTTTTGAATTGACGCAAAGCGAag                                                                                                              >  1:804015/1‑140 (MQ=255)

                       ttGCTGGCCGTGGTTTGGTCCGGCGGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCACTGACTTTTGAATTGACGCAAAGCGAag                                                                                                              >  1:737735/1‑140 (MQ=255)

                          cTGGCCGTGGTTTGGTCCGGCGGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCACTGACTTTTGAATTGACGCAAAGCGAAgaga                                                                                                           >  2:616541/1‑140 (MQ=255)

                            ggCCGTGGTTTGGTCCGGCGGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCACTGACTTTTGAATTGACGCAAAGCGAAGAGACg                                                                                                         >  1:85500/1‑140 (MQ=255)

                             gCCGTGGTTTGGTCCGGCGGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCACTGACTTTTGAATTGACGCAAAGCGAAGAGACGa                                                                                                        >  2:662630/1‑140 (MQ=255)

                                gTGGTTTGGTCCGGCGGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCACTGACTTTTGAATTGACGCAAAGCGAAGAGACGaaaa                                                                                                     >  1:1425391/1‑140 (MQ=255)

                                             gcggcACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCACTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCa                                                                                        <  1:662630/140‑1 (MQ=255)

                                             gcggcACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCACTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCa                                                                                        >  2:665097/1‑140 (MQ=255)

                                                        aGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCACTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGc                                                                             <  2:897793/140‑1 (MQ=255)

                                                           tCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCACTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACg                                                                              >  2:174720/1‑136 (MQ=255)

                                                           tCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCACTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACg                                                                              <  1:174720/136‑1 (MQ=255)

                                                             tgcctgcGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCACTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTa                                                                        >  1:750291/1‑140 (MQ=255)

                                                                ctgcGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCACTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAgcg                                                                     >  1:122452/1‑140 (MQ=255)

                                                                            ttGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCACTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTg                                                         <  2:753617/140‑1 (MQ=255)

                                                                             tGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCACTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCcgcg                                                           <  1:907065/137‑1 (MQ=255)

                                                                             tGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCACTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCcgcg                                                           >  2:907065/1‑137 (MQ=255)

                                                                               cccGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCACTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGt                                                      <  1:1156284/140‑1 (MQ=255)

                                                                                    aaCCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCACTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCcgcg                                                           >  1:1296763/1‑130 (MQ=255)

                                                                                    aaCCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCACTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCcgcg                                                           <  2:1296763/130‑1 (MQ=255)

                                                                                             tGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCACTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAAt                                        <  1:616541/140‑1 (MQ=255)

                                                                                             tGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCACTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAAt                                        <  1:665097/140‑1 (MQ=255)

                                                                                             tGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCACTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAAt                                        <  2:737735/140‑1 (MQ=255)

                                                                                               gACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCACTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGATGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCg                                      >  2:394865/1‑140 (MQ=255)

                                                                                                aCGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCACTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGa                                     >  2:702705/1‑140 (MQ=255)

                                                                                                    tGAAATCACATCATGAAGATGCTGATGGCGTAGCACTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCtt                                 <  1:1442867/140‑1 (MQ=255)

                                                                                                                 tGAAGATGCTGATGGCGTAGCACTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCg                    >  2:1127091/1‑140 (MQ=255)

                                                                                                                   aaGATGCTGATGGCGTAGCACTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGaa                  >  2:1181889/1‑140 (MQ=255)

                                                                                                                       tGCTGATGGCGTAGCACTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTg              >  1:274264/1‑140 (MQ=255)

                                                                                                                       tGCTGATGGCGTAGCACTGAC‑TTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGa             >  1:1299858/1‑140 (MQ=255)

                                                                                                                         cTGATGGCGTAGCACTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGaa            <  2:85500/140‑1 (MQ=255)

                                                                                                                          tGATGGCGTAGCACTGAC‑TTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAac          <  2:1299858/140‑1 (MQ=255)

                                                                                                                           gATGGCGTAGCACTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAac          >  2:1298414/1‑140 (MQ=255)

                                                                                                                           gATGGCGTAGCACTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAac          <  2:509883/140‑1 (MQ=255)

                                                                                                                           gATGGCGTAGCACTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGGAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAac          <  1:394865/140‑1 (MQ=255)

                                                                                                                            aTGGCGTAGCACTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAacc         >  1:539775/1‑140 (MQ=255)

                                                                                                                                  tAGCACTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACctct   >  1:419770/1‑140 (MQ=255)

                                                                                                                                  tAGCACTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACctct   <  2:1425391/140‑1 (MQ=255)

                                                                                                                                   aGCACTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAac          <  2:918963/132‑1 (MQ=255)

                                                                                                                                   aGCACTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAac          <  2:657220/132‑1 (MQ=255)

                                                                                                                                   aGCACTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAac          >  1:918963/1‑132 (MQ=255)

                                                                                                                                   aGCACTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAac          <  1:770852/132‑1 (MQ=255)

                                                                                                                                   aGCACTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAac          >  1:657220/1‑132 (MQ=255)

                                                                                                                                   aGCACTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTg  <  2:541050/140‑1 (MQ=255)

                                                                                                                                   aGCACTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTg  <  1:670242/140‑1 (MQ=255)

GCTATTCGCGGTGGCGTACCGGTTTGCTGGCCGTGGTTTGGTCCGGCGGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTG  >  NC_000913/1864063‑1864333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A