Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F28 I1 R1	151	87.4	2971636	99.2%	2947862	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,119,480	T→C	T227A (ACC→GCC)	wzxC ←	M‑antigen undecaprenyl disphosphate flippase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,119,480	0	T	C	100.0%	162.5 / NA	46	T227A (ACC→GCC)	wzxC	M‑antigen undecaprenyl disphosphate flippase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (24/22); total (24/22)

CCGGAAACAACACGCGGGTGATGATTGGGTTCAGCTTCATCGGTGGCACAACGGCCACGTTGTACGCCAGGTTGTATCCCCCTGCCACGCCCGCGCCGAGAATACGCGCCAGCACGAGCGTTGAAAGGTTGGTATTGAGATAGTTGATGATGCTGTCCGCCGTCAGCCAGGCACCAAAGCGTAAGTTCGGTGCCACCGACGCCAGCGAGAAATGCAGACCGGGGCGATAAATTTTGCGGCCAAAGTAGCCAAACAGCAGCGTTCTCA  >  NC_000913/2119348‑2119614

ccGGAAACAACACGCGGGTGATGATTGGGTTCAGCTTCATGGGTGGCACAACGGCCACGTTGTACGCCAGGTTGTATCCCCCTGCCACGCCCGCGCCGAGAATACGCGCCAGCACGAGCGTTGAAAGGTTGGCATTgaga                                                                                                                                 <  2:576625/140‑1 (MQ=255)

ccGGAAACAACACGCGGGTGATGATTGGGTTCAGCTTCATCGGTGGCACAACGGCCACGTTGTACGCCAGGTTGTATCCCCCTGCCACGCCCGCGCCGAGAATACGCGCCAGCACGAGCGTTGAAAGGTTGGCATTgaga                                                                                                                                 <  1:1173304/140‑1 (MQ=255)

         acacGCGGGTGATGATTGGGTTCAGCTTCATCGGTGGCACAACGGCCACGTTGTACGCCAGGTTGTATCCCCCTGCCACGCCCGCGCCGAGAATACGCGCCAGCACGAGCGTTGAAAGGTTGGCATTGAGATAGTtgatg                                                                                                                        <  1:643218/140‑1 (MQ=255)

                ggTGATGATTGGGTTCAGCTTCATCGGTGGCACAACGGCCACGTTGTACGCCAGGTTGTATCCCCCTGCCACGCCCGCGCCGAGAATACGCGCCAGCACGAGCGTTGAAAGGTTGGCATTGAGATAGTTGATGATGCTGt                                                                                                                 >  2:399319/1‑140 (MQ=255)

                 gTGATGATTGGGTTCAGCTTCATCGGTGGCACAACGGCCACGTTGTACGCCAGGTTGTATCCCCCTGCCACGCCCGCGCCGAGAATACGCGCCAGCACGAGCGTTGAAAGGTTGGCATTGAGATAGTTGATGATGCTGTc                                                                                                                >  2:627452/1‑140 (MQ=255)

                     tgtttGGGTTCAGCTTCATCGGTGGCACAACGGCCACGTTGTACGCCAGGTTGTATCCCCCTGCCACGCCCGCGCCGAGAATACGCGCCAGCACGAGCGTTGAAAGGTTGGCATTGAGATAGTTGATGATGCTGTccgcc                                                                                                            <  2:737928/137‑1 (MQ=255)

                         tGGGTTCAGCTTCATCGGTGGCACAACGGCCACGTTGTACGCCAGGTTGTATCCCCCTGCCACGCCCGCGCCGAGAATACGCGCCAGCACGAGCGTTGAAAGGTTGGCATTGAGATAGTTGatgatg                                                                                                                     >  2:901916/1‑127 (MQ=255)

                         tGGGTTCAGCTTCATCGGTGGCACAACGGCCACGTTGTACGCCAGGTTGTATCCCCCTGCCACGCCCGCGCCGAGAATACGCGCCAGCACGAGCGTTGAAAGGTTGGCATTGAGATAGTTGatgatg                                                                                                                     <  1:901916/127‑1 (MQ=255)

                         tGGGTTCAGCTTCATCGGTGGCACAACGGCCACGTTGTACGCCAGGTTGTATCCCCCTGCCACGCCCGCGCCGAGAATACGCGCCAGCACGAGCGTTGAAAGGTTGGCATTGAGATAGTTGATGATGCTGTCCGCCGTCa                                                                                                        >  1:413376/1‑140 (MQ=255)

                          gggTTCAGCTTCATCGGTGGCACAACGGCCACGTTGTACGCCAGGTTGTATCCCCCTGCCACGCCCGCGCCGAGAATACGCGCCAGCACGAGCGTTGAAAGGTTGGCATTGAGATAGTTGATGATGCTGTCCGCCGTCAg                                                                                                       >  1:1223633/1‑140 (MQ=255)

                          gggTTCAGCTTCATCGGTGGCACAACGGCCACGTTGTACGCCAGGTTGTATCCCCCTGCCACGCCCGCGCCGAGAATACGCGCCAGCACGAGCGTTGAAAGGTTGGCATTGAGATAGTTGATGATGCTGTCCGCCGTCAg                                                                                                       <  2:1223633/140‑1 (MQ=255)

                           ggTTCAGCTTCATCGGTGGCACAACGGCCACGTTGTACGCCAGGTTGTATCCCCCTGCCACGCCCGCGCCGAGAATACGCGCCAGCACGAGCGTTGAAAGGTTGGCATTGAGATAGTTGATGATGCTGTCCGCCGTCAGc                                                                                                      >  2:1225497/1‑140 (MQ=255)

                           ggTTCAGCTTCATCGGTGGCACAACGGCCACGTTGTACGCCAGGTTGTATCCCCCTGCCACGCCCGCGCCGAGAATACGCGCCAGCACGAGCGTTGAAAGGTTGGCATTGAGATAGTTGATGATGCTGTCCGCCGTCAGc                                                                                                      >  2:217837/1‑140 (MQ=255)

                                 gCTTCATCGGTGGCACAACGGCCACGTTGTACGCCAGGTTGTATCCCCCTGCCACGCCCGCGCCGAGAATACGCGCCAGCACGAGCGTTGAAAGGTTGGCATTGAGATAGTTGATGATGCTGTCCGCCGTCAGCCAGGCa                                                                                                >  1:1385615/1‑140 (MQ=255)

                                  cTTCATCGGTGGCACAACGGCCACGTTGTACGCCAGGTTGTATCCCCCTGCCACGCCCGCGCCGAGAATACGCGCCAGCACGAGCGTTGAAAGGTTGGCATTGAGATAGTTGATGATGCTGTCCGCCGTCAGCCAGGCAc                                                                                               <  1:13308/140‑1 (MQ=255)

                                    tCATCGGTGGCACAACGGCCACGTTGTACGCCAGGTTGTATCCCCCTGCCACGCCCGCGCCGAGAATACGCGCCAGCACGAGCGTTGAAAGGTTGGCATTGAGATAGTTGATGATGCTGTCCGCCGTCAGCCAGGCACCa                                                                                             <  2:672521/140‑1 (MQ=255)

                                     cATCGGTGGCACAACGGCCACGTTGTACGCCAGGTTGTATCCCCCTGCCACGCCCGCGCCGAGAATACGCGCCAGCACGAGCGTTGAAAGGTTGGCATTGAGATAGTTGATGATGCTGTCCGCCGTCAGCCAGGCACCaa                                                                                            <  1:1269645/140‑1 (MQ=255)

                                     cATCGGTGGCACAACGGCCACGTTGTACGCCAGGTTGTATCCCCCTGCCACGCCCGCGCCGAGAATACGCGCCAGCACGAGCGTTGAAAGGTTGGCATTGAGATAGTTGATGATGCTGTCCGCCGTCAGCCAGGCACCaa                                                                                            <  1:627452/140‑1 (MQ=255)

                                      aTCGGTGGCACAACGGCCACGTTGTACGCCAGGTTGTATCCCCCTGCCACGCCCGCGCCGAGAATACGCGCCAGCACGAGCGTTGAAAGGTTGGCATTGAGATAGTTGATGATGCTGTCCGCCGTCAGCCAGGCACCaaa                                                                                           >  2:1111643/1‑140 (MQ=255)

                                          gTGGCACAACGGCCACGTTGTACGCCAGGTTGTATCCCCCTGCCACGCCCGCGCCGAGAATACGCGCCAGCACGAGCGTTGAAAGGTTGGCATTGAGATAGTTGATGATGCTGTCCGCCGTCAGCTAGGCACCAAAGCGt                                                                                       <  1:217837/140‑1 (MQ=255)

                                            ggCACAACGGCCACGTTGTACGCCAGGTTGTATCCCCCTGCCACGCCCGCGCCGAGAATACGCGCCAGCACGAGCGTTGAAAGGTTGGCATTGAGATAGTTGATGATGCTGTCCGCCGTCAGCCAGGCACCAAAGCGTaa                                                                                     >  2:297043/1‑140 (MQ=255)

                                             gCACAACGGCCACGTTGTACGCCAGGTTGTATCCCCCTGCCACGCCCGCGCCGAGAATACGCGCCAGCACGAGCGTTGAAAGGTTGGCATTGAGATAGTTGATGATGCTGTCCGCCGTCAGCCAGGCACCAAAGCGTAAg                                                                                    <  2:306320/140‑1 (MQ=255)

                                               acaACGGCCACGTTGTACGCCAGGTTGTATCCCCCTGCCACGCCCGCGCCGAGAATACGCGCCAGCACGAGCGTTGAAAGGTTGGCATTGAGATAGTTGATGATGCTGTCCGCCGTCAGCCAGGCACCAAAGCGTAAGtt                                                                                  <  1:517123/140‑1 (MQ=255)

                                               acaACGGCCACGTTGTACGCCAGGTTGTATCCCCCTGCCACGCCCGCGCCGAGAATACGCGCCAGCACGAGCGTTGAAAGGTTGGCATTGAGATAGTTGATGATGCTGTCCGCCGTCAGCCAGGCACCAAAGCGTAAGtt                                                                                  <  1:164726/140‑1 (MQ=255)

                                                  aCGGCCACGTTGTACGCCAGGTTGTATCCCCCTGCCACGCCCGCGCCGAGAATACGCGCCAGCACGAGCGTTGAAAGGTTGGCATTGAGATAGTTGATGATGCTGTCCGCCGTCAGCCAGGCACCAAAGCGTAAGTTCgg                                                                               <  1:1029160/140‑1 (MQ=255)

                                                             gTACGCCAGGTTGTATCCCCCTGCCACGCCCGCGCCGAGAATACGCGCCAGCACGAGCGTTGAAAGGTTGGCATTGAGATAGTTGATGATGCTGTCCGCCGTCAGCCAGGCACCAAAGCGTAAGTTCGGTGCCACCGACg                                                                    <  1:399319/140‑1 (MQ=255)

                                                              tACGCCAGGTTGTATCCCCCTGCCACGCCCGCGCCGAGAATACGCGCCAGCACGAGCGTTGAAAGGTTGGCATTGAGATAGTTGATGATGCTGTCCGCCGTCAGCCAGGCACCAAAGCGTAAGTTCGGTGCCACCGACGc                                                                   >  2:323827/1‑140 (MQ=255)

                                                                     ggTTGTATCCCCCTGCCACGCCCGCGCCGAGAATACGCGCCAGCACGAGCGTTGAAAGGTTGGCATTGAGATAGTTGATGATGCTGTCCGCCGTCAGCCAGGCACCAAAGCGTAAGTTCGGTGCCACCGACGCCAGCgag                                                            <  1:1225497/140‑1 (MQ=255)

                                                                          tATCCCCCTGCCACGCCCGCGCCGAGAATACGCGCCAGCACGAGCGTTGAAAGGTTGGCATTGAGATAGTTGATGATGCTGTCCGCCGTCAGCCAGGCACCAAAGCGTAAGTTCGGTGCCACCGACGCCAGCGAGAAATg                                                       >  2:16865/1‑140 (MQ=255)

                                                                               cccTGCCACGCCCGCGCCGAGAATACGCGCCAGCACGAGCGTTGAAAGGTTGGCATTGAGATAGTTGATGATGCTGTCCGCCGTCAGCCAGGCACCAAAGCGTAAGTTCGGTGCCACCGACGCCAGCGAGAAATGCAGAc                                                  >  2:147168/1‑140 (MQ=255)

                                                                                 cTGCCACGCCCGCGCCGAGAATACGCGCCAGCACGAGCGTTGAAAGGTTGGCATTGAGATAGTTGATGATGCTGTCCGCCGTCAGCCAGGCACCAAAGCGTAAGTTCGGTGCCACCGACGCCAGCGAGAAATGCAGACCg                                                >  1:1153580/1‑140 (MQ=255)

                                                                                 cTGCCACGCCCGCGCCGAGAATACGCGCCAGCACGAGCGTTGAAAGGTTGGCATTGAGATAGTTGATGATGCTGTCCGCCGTCAGCCAGGCACCAAAGCGTAAGTTCGGTGCCACCGACGCCAGCGAGAAATGCAGACCg                                                >  1:1079272/1‑140 (MQ=255)

                                                                                    ccACGCCCGCGCCGAGAATACGCGCCAGCACGAGCGTTGAAAGGTTGGCATTGAGATAGTTGATGATGCTGTCCGCCGTCAGCCAGGCACCAAAGCGTAAGTTCGGTGCCACCGACGCCAGCGAGAAATGCAGACCgggg                                             >  1:427014/1‑140 (MQ=255)

                                                                                             cgcCGAGAATACGCGCCAGCACGAGCGTTGAAAGGTTGGCATTGAGATAGTTGATGATGCTGTCCGCCGTCAGCCAGGCACCAAAGCGTAAGTTCGGTGCCACCGACGCCAGCGAGAAATGCAGACCGGGGCGATAAAtt                                    >  1:520846/1‑140 (MQ=255)

                                                                                             cgcCGAGAATACGCGCCAGCACGAGCGTTGAAAGGTTGGCATTGAGATAGTTGATGATGCTGTCCGCCGTCAGCCAGGCACCAAAGCGTAAGTTCGGTGCCACCGACGCCAGCGAGAAATGCAGACCGGGGCGATAAAtt                                    >  1:300123/1‑140 (MQ=255)

                                                                                               ccGAGAATACGCGCCAGCACGAGCGTTGAAAGGTTGGCATTGAGATAGTTGATGATGCTGTCCGCCGTCAGCCAGGCACCAAAGCGTAAGTTCGGTGCCACCGACGCCAGCGAGAAATGCAGACCGGGGCGATAAAtttt                                  <  2:1153580/140‑1 (MQ=255)

                                                                                                   gaATACGCGCCAGCACG‑GCGTTGAAAGGTTGGCATTGAGATAGTTGATGATGCTGTCCGCCGTCAGCCAGGCACCAAAGCGTAAGTTCGGTGCCACCGGCGCCCGCGAGAAATGCAGACCGGGGCGATAAATTTTGCGGc                             <  2:952732/140‑1 (MQ=255)

                                                                                                    aaTACGCGCCAGCACGAGCGTTGAAAGGTTGGCATTGAGATAGTTGATGATGCTGTCCGCCGTCAGCCAGGCACCAAAGCGTAAGTTCGGTGCCACCGACGCCAGCGAGAAATGCAGACCGGGGCGATAAATTTTGCGGc                             >  1:87298/1‑140 (MQ=255)

                                                                                                              aGCACGAGCGTTGAAAGGTTGGCATTGAGATAGTTGATGATGCTGTCCGCCGTCAGCCAGGCACCAAAGCGTAAGTTCGGTGCCACCGACGCCAGCGAGAAATGCAGACCGGGGCGATAAATTTTGCGGCCAAAGTAGcc                   >  2:173464/1‑140 (MQ=255)

                                                                                                              aGCACGAGCGTTGAAAGGTTGGCATTGAGATAGTTGACGATGCTGTCCGCCGTCAGCCAGGCACCAAAGCGTAAGTTCGGTGCCACCGACGCCAGCGAGAAATGCAGACCGGGGCGATAAATTTTGCGGCCAAAGTAGcc                   >  2:26745/1‑140 (MQ=255)

                                                                                                                   gAGCGTTGAAAGGTTGGCATTGAGATAGTTGATGATGCTGTCCGCCGTCAGCCAGGCACCAAAGCGTAAGTTCGGTGCCACCGACGCCAGCGAGAAATGCAGACCGGGGCGATAAATTTTGCGGCCAAAGTAGCCAAAca              <  2:1079272/140‑1 (MQ=255)

                                                                                                                      cGTTGAAAGGTTGGCATTGAGATAGTTGATGATGCTGTCCGCCGTCAGCCAGGCACCAAAGCGTAAGTTCGGTGCCACCGACGCCAGCGAGAAATGCAGACCGGGGCGATAAATTTTGCGGCCAAAGTAGCCAAAcagca           <  2:743811/140‑1 (MQ=255)

                                                                                                                           aaaGGTTGGCATTGAGATAGTTGATGATGCTGTCCGCCGTCAGCCAGGCACCAAAGCGTAAGTTCGGTGCCACCGACGCCAGCGAGAAATGCAGACCGGGGCGATAAATTTTGCGGCCAAAGTAGCCAAACAGCAGCGtt      >  1:847749/1‑140 (MQ=255)

                                                                                                                             aGGTTGGCATTGAGATAGTTGATGATGCTGTCCGCCGTCAGCCAGGCACCAAAGCGTAAGTTCGGTGCCACCGACGCCAGCGAGAAATGCAGACCGGGGCGATAAATTTTGCGGCCAAAGTAGCCAAACAGCAGCGTtct    <  2:300123/140‑1 (MQ=255)

                                                                                                                               gTTGGCATTGAGATAGTTGATGATGCTGTCCGCCGTCAGCCAGGCACCAAAGCGTAAGTTCGGTGCCACCGACGCCAGCGAGAAATGCAGACCGGGGCGATAAATTTTGCGGCCAAAGTAGCCAAAca              >  2:1180885/1‑128 (MQ=255)

                                                                                                                               gTTGGCATTGAGATAGTTGATGATGCTGTCCGCCGTCAGCCAGGCACCAAAGCGTAAGTTCGGTGCCACCGACGCCAGCGAGAAATGCAGACCGGGGCGATAAATTTTGCGGCCAAAGTAGCCAAAca              <  1:1180885/128‑1 (MQ=255)

                                                                                                                               gTTGGCATTGAGATAGTTGATGATGCTGTCCGCCGTCAGCCAGGCACCAAAGCGTAAGTTCGGTGCCACCGACGCCAGCGAGAAATGCAGACCGGGGCGATAAATTTTGCGGCCAAAGTAGCCAAACAGCAGCGTTCTCa  >  2:1247185/1‑140 (MQ=255)

CCGGAAACAACACGCGGGTGATGATTGGGTTCAGCTTCATCGGTGGCACAACGGCCACGTTGTACGCCAGGTTGTATCCCCCTGCCACGCCCGCGCCGAGAATACGCGCCAGCACGAGCGTTGAAAGGTTGGTATTGAGATAGTTGATGATGCTGTCCGCCGTCAGCCAGGCACCAAAGCGTAAGTTCGGTGCCACCGACGCCAGCGAGAAATGCAGACCGGGGCGATAAATTTTGCGGCCAAAGTAGCCAAACAGCAGCGTTCTCA  >  NC_000913/2119348‑2119614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A