Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F28 I1 R1 151 87.4 2971636 99.2% 2947862 138.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,402,681 A→C S389A (TCT→GCT)  nuoC ← NADH:quinone oxidoreductase subunit CD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,402,6810AC100.0% 201.3 / NA 57S389A (TCT→GCT) nuoCNADH:quinone oxidoreductase subunit CD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (31/26);  total (31/26)

TCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGGCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGACGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGCAGCAGGCGATCCCAGCCGCGCGGCAGGTCGTGCGCTACGCCGCCAATACGGAACCACGCCGGGTGCATACGGAAACCAGTGATTGCTTCCACCAGAT  >  NC_000913/2402548‑2402815
                                                                                                                                     |                                                                                                                                      
tCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGCGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGGCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGCCGCCAg                                                                                                                                  >  2:1451778/1‑140 (MQ=255)
tCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGCGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGGCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGCCGCCAg                                                                                                                                  >  2:913283/1‑140 (MQ=255)
 cGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGCGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGGCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGCCGCCAGa                                                                                                                                 <  2:966770/140‑1 (MQ=255)
                   cACGCAGGCCCGCGCCAGCGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGGCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCg                                                                                                               >  2:580503/1‑140 (MQ=255)
                      gCAGGCCCGCGCCAGCGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGGCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAgg                                                                                                            >  1:1201190/1‑140 (MQ=255)
                          gCCCGCGCCAGCGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGGCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACt                                                                                                        >  2:655485/1‑140 (MQ=255)
                            ccGCGCCAGCGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGGCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCa                                                                                                      <  2:816893/140‑1 (MQ=255)
                               cgcCAGCGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGGCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACgc                                                                                                   >  1:1458677/1‑140 (MQ=255)
                                gcCAGCGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGGCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACgca                                                                                                  <  2:1201190/140‑1 (MQ=255)
                                gcCAGCGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGGCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACgca                                                                                                  <  2:1088498/140‑1 (MQ=255)
                                 ccAGCGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGGCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTTTTTTGCAGCGCCGCTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACgcag                                                                                                 <  2:1309953/140‑1 (MQ=255)
                                 ccAGCGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGGCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACgcag                                                                                                 <  1:643708/140‑1 (MQ=255)
                                         gCCCCACTCCAGCGCCTCTTTCGCGCCATAGGCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGCAGCAGGCGAt                                                                                         <  1:1451778/140‑1 (MQ=255)
                                               cTCCAGCGCCTCTTTCGCGCCATAGGCGGCAACGCCCTGGGAACGACCTTTCAGAATG‑‑GTTTTGCAGCGCCGCTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGCAGCAGGCGATCCCAGCcg                                                                                 >  1:650070/1‑140 (MQ=255)
                                                tCCAGCGCCTCTTTCGCGCCATAGGCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGCAGCAGGCGATCCCAGcc                                                                                  >  2:964472/1‑140 (MQ=255)
                                                tCCAGCGCCTCTTTCGCGCCATAGGCGGCAACGCCCTGGGAACGACCTTTCAGAATG‑‑GTTTTGCAGCGCCGCTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGCAGCAGGCGATCCCAGCcgc                                                                                <  2:650070/140‑1 (MQ=255)
                                                     cgcCTCTTTCGCGCCATAGGCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGCAGCAGGCGATCCCAGCcgcgcg                                                                             >  1:1084953/1‑140 (MQ=255)
                                                        ctctTTCGCGCCATAGGCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGCAGCAGGCGATCCCAGCCGCGCGGCa                                                                          >  2:1013351/1‑140 (MQ=255)
                                                          ctTTCGCGCCATAGGCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGCAGCAGGCGATCCCAGCCGCGCGGCAgg                                                                        >  1:360081/1‑140 (MQ=255)
                                                          ctTTCGCGCCATAGGCGGCAACGCCCTGGGAACGACC‑TTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGCAGCAGGCGATCCCAGCCGCGCGGCAGGt                                                                       >  1:938982/1‑140 (MQ=255)
                                                               gcgcCATAGGCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGCAGCAGGCGATCCCAGCCGCGCGGCAGGTCGTg                                                                   >  1:1204306/1‑140 (MQ=255)
                                                                        gcggcAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGCAGCAGGCGATCCCAGCCGCGCGGCAGGTCGTGCGCTAcgcc                                                          <  2:1084953/140‑1 (MQ=255)
                                                                          ggcAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGCAGCAGGCGATCCCAGCCGCGCGGCAGGTCGTGCGCTAcgccgc                                                        <  2:442473/140‑1 (MQ=255)
                                                                          ggcAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGCAGCAGGCGATCCCAGCCGCGCGGCAGGTCGTGCGCTAcgccgc                                                        <  1:860121/140‑1 (MQ=255)
                                                                           gcAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGCAGCAGGCGATCCCAGCCGCGCGGCAGGTCGTGCGCTACgccgcc                                                       >  2:145573/1‑140 (MQ=255)
                                                                             aaCGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGCAGCAGGCGATCCCAGCCGCGCGGCAGGTCGTGCGCTACGCCGCCaa                                                     >  2:297314/1‑140 (MQ=255)
                                                                               cGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGCAGCAGGCGATCCCAGCCGCGCGGCAGGTCGTGCGCTAcgccgc                                                        >  1:1192347/1‑135 (MQ=255)
                                                                               cGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGCAGCAGGCGATCCCAGCCGCGCGGCAGGTCGTGCGCTAcgccgc                                                        <  2:1192347/135‑1 (MQ=255)
                                                                               cGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGCAGCAGGCGATCCCAGCCGCGCGGCAGGTCGTGCGCTACGCCGCCAATa                                                   <  2:832960/140‑1 (MQ=255)
                                                                                 cccTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGCAGCAGGCGATCCCAGCCGCGCGGCAGGTCGTGCGCTACGCCGCCAATACg                                                 >  1:266011/1‑140 (MQ=255)
                                                                                  ccTGGGAACGACCTTTCAGAAAGGTGTTTTGCAGCGCCGCTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGCAGCAGGCGATCCCAGCCGCGCGGCAGGTCGTGCGCTACGCCGCCAATACgg                                                >  2:1056606/1‑140 (MQ=255)
                                                                                          cGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGCAGCAGGCGATCCCAGCCGCGCGGCAGGTCGTGCGCTACGCCGCCAATa                                                   <  1:913570/129‑1 (MQ=255)
                                                                                          cGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGCAGCAGGCGATCCCAGCCGCGCGGCAGGTAGTGCGCTACGCCGCCAATa                                                   >  2:913570/1‑129 (MQ=255)
                                                                                            aCCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGCAGCAGGCGATCCCAGCCGCGCGGCAGGTCGTGCGCTACGCCGCCAATACGGAACCACGCCg                                      >  1:1116477/1‑140 (MQ=255)
                                                                                            aCCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGCAGCAGGCGATCCCAGCCGCGCGGCAGGTCGTGCGCTACGCCGCCAATACGGAACCACGCCg                                      >  1:1175614/1‑140 (MQ=255)
                                                                                               tttCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGCAGCAGGCGATCCCAGCCGCGCGGCAGGTCGTGCGCTACGCCGCCAATACGGAACCACGCCGGGt                                   <  1:937681/140‑1 (MQ=255)
                                                                                                ttCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGCAGCAGGCGATCCCAGCCGCGCGGCAGGTCGTGCGCTACGCCGCCAATACGGAACCACGCCGGGTg                                  >  1:761435/1‑140 (MQ=255)
                                                                                                 tCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGCAGCAGGCGATCCCGGCCGCGCGGCAGGTCGTGCGCTACGCCGCC‑ATACGGAACCACGCCGGGTGCa                                >  1:1182333/1‑140 (MQ=255)
                                                                                                 tCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGCAGCAGGCGATCCCAGCCGCGCGGCAGGTCGTGCGCTACGCCGCCAATACGGAACCACGCCGGGTGc                                 <  1:1013351/140‑1 (MQ=255)
                                                                                                       tGGTGTTTTGCAGCGCCGCTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGCAGCAGGCGATCCCAGCCGCGCGGCAGGTCGTGCGCTACGCCGCCAATACGGAACCACGc                                        <  1:112644/127‑1 (MQ=255)
                                                                                                       tGGTGTTTTGCAGCGCCGCTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGCAGCAGGCGATCCCAGCCGCGCGGCAGGTCGTGCGCTACGCCGCCAATACGGAACCACGc                                        >  2:112644/1‑127 (MQ=255)
                                                                                                       tGGTGTTTTGCAGCGCCGCTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGCAGCAGGCGATCCCAGCCGCGCGGCAGGTCGTGCGCTACGCCGCCAATACGGAACCACGCCGGGTGCATACgg                           >  1:746529/1‑140 (MQ=255)
                                                                                                       tGGTGTTTTGCAGCGCCGCTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGCAGCAGGCGATCCCAGCCGCGCGGCAGGTCGTGCGCTACGCCGCCAATACGGAACCACGCCGGGTGCATACgg                           >  2:537129/1‑140 (MQ=255)
                                                                                                          tgtTTTGCAGCGCCGCTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGCAGCAGGCGATCCCAGCCGCGCGGCAGGTCGTGCGCTACGCCGCCAATACGGAACCACGCCGGGTGCATACGGaaa                        >  1:742688/1‑140 (MQ=255)
                                                                                                           gtTTTGCAGCGCCGCTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGCAGCAGGCGATCCCAGCCGCGCGGCAGGTCGTGCGCTACGCCGCCAATACGGAACCACGCCGGGTGCATACGGAAAc                       >  1:1253877/1‑140 (MQ=255)
                                                                                                            ttttGCAGCGCCGCTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGCAGCAGGCGATCCCAGCCGCGCGGCAGGTCGTGCGCTACGCCGCCAATACGGAACCACGCCGGGTGCATACGGAAAcc                      >  2:16182/1‑140 (MQ=255)
                                                                                                                 cAGCGCCGCTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGCAGCAGGCGATCCCAGCCGCGCGGCAGGTCGTGCGCTACGCCGCCAATACGGAACCACGCCGGGTGCATACGGAAACCAGTGa                 <  2:1175614/140‑1 (MQ=255)
                                                                                                                 cAGCGCCGCTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGCAGCAGGCGATCCCAGCCGCGCGGCAGGTCGTGCGCTACGCCGCCAATACGGAACCACGCCGGGTGCATACGGAAACCAGTGa                 <  2:1116477/140‑1 (MQ=255)
                                                                                                                 cAGCGCCGCTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGCAGCAGGCGATCCCAGCCGCGCGGCAGGTCGTGCGCTACGCCGCCAATACGGAACCACGCCGGGTGCATACGGAAACCAGTGa                 <  1:537129/140‑1 (MQ=255)
                                                                                                                 cAGCGCCGCTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGCAGCAGGCGATCCCAGCCGCGCGGCAGGTCGTGCGCTACGCCGCCAATACGGAACCACGCCGGGTGCATACGGAAACCAGTGa                 <  1:297314/140‑1 (MQ=255)
                                                                                                                   gcgcCGCTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGCAGCAGGCGATCCCAGCCGCGCGGCAGGTCGTGCGCTACGCCGCCAATACGGAACCACGCCGGGTGCATACGGAAACCAGTGAtt               <  1:655485/140‑1 (MQ=255)
                                                                                                                         cTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGCAGCAGGCGATCCCAGCCGCGCGGCAGGTCGTGCGCTACGCCGCCAATACGGAACCACGCCGGGTGCATACGGAAACCAGTGATTGCt            <  1:1201931/137‑1 (MQ=255)
                                                                                                                         cTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGCAGCAGGCGATCCCAGCCGCGCGGCAGGTCGTGCGCTACGCCGCCAATACGGAACCACGCCGGGTGCATACGGAAACCAGTGATTGCt            >  2:1201931/1‑137 (MQ=255)
                                                                                                                         cTTTCTCGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGCAGCAGGCGATCCCAGCCGCGCGGCAGGTCGTGCGCTACGCCGCCAATACGGAACCACGCCGGGTGCATACGGAAACCAGTGATTGCTTcc         >  1:937952/1‑140 (MQ=255)
                                                                                                                            tctcGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGCAGCAGGCGATCCCAGCCGCGCGGCAGGTCGTGCGCTACGCCGCCAATACGGAACCACGCCGGGTGCATACGGAAACCAGTGATTGCTTccacc      <  2:937952/140‑1 (MQ=255)
                                                                                                                             ctcGTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGCAGCAGGCGATCCCAGCCGCGCGGCAGGTCGTGCGCTACGCCGCCAATACGGAACCACGCCGGGTGCATACGGAAACCAGTGATTGCTTccacca     <  2:938982/140‑1 (MQ=255)
                                                                                                                                gTAAGCCGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGCAGCAGGCGATCCCAGCCGCGCGGCAGGTCGTGCGCTACGCCGCCAATACGGAACCACGCCGGGTGCATACGGAAACCAGTGATTGCTTCCACCAGAt  <  1:758862/140‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                      
TCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGGCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGACGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGCAGCAGGCGATCCCAGCCGCGCGGCAGGTCGTGCGCTACGCCGCCAATACGGAACCACGCCGGGTGCATACGGAAACCAGTGATTGCTTCCACCAGAT  >  NC_000913/2402548‑2402815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A