Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F28 I1 R1	151	87.4	2971636	99.2%	2947862	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,765,794	(C)_7→8	intergenic (‑18/‑124)	yfjM ← / → rnlA	CP4‑57 prophage; protein YfjM/CP4‑57 prophage; RNase LS, toxin of the RnlAB toxin‑antitoxin system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,765,787	1	.	C	94.4%	235.2 / 5.8	71	intergenic (‑11/‑131)	yfjM/rnlA	CP4‑57 prophage; protein YfjM/CP4‑57 prophage; RNase LS, toxin of the RnlAB toxin‑antitoxin system
Reads supporting (aligned to +/- strand): ref base . (1/3); new base C (28/39); total (29/42)
Fisher's exact test for biased strand distribution p-value = 6.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGGCTCTCTGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGG  >  NC_000913/2765648‑2765915

tGGGCTCTCTGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑Tg                                                                                                                                   <  1:363412/140‑1 (MQ=255)

   gCTCTCTGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TG‑ccc                                                                                                                               <  1:606277/140‑1 (MQ=255)

    ctctctGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TG‑cccc                                                                                                                              <  1:768153/140‑1 (MQ=255)

      ctctGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TG‑cccccc                                                                                                                            <  1:1340131/140‑1 (MQ=255)

       tctGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TG‑ccccccc                                                                                                                           <  2:617220/140‑1 (MQ=255)

       tctGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TG‑ccccccc                                                                                                                           >  2:1034280/1‑140 (MQ=255)

       tctGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TG‑ccccccc                                                                                                                           <  1:548860/140‑1 (MQ=255)

        ctGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TG‑cccccccc                                                                                                                          <  1:1189488/140‑2 (MQ=255)

                   gTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATTAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATc                                                                                                                <  2:871797/140‑1 (MQ=255)

                   gTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATc                                                                                                                <  1:1070563/140‑1 (MQ=255)

                   gTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATc                                                                                                                <  2:984886/140‑1 (MQ=255)

                   gTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATc                                                                                                                <  2:65933/140‑1 (MQ=255)

                   gTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATc                                                                                                                <  2:1194500/140‑1 (MQ=255)

                   gTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATc                                                                                                                <  1:1403849/140‑1 (MQ=255)

                   gTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TG‑cc                                                                                                                                <  1:1419920/123‑1 (MQ=255)

                   gTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TG‑cc                                                                                                                                >  2:1419920/1‑123 (MQ=255)

                   gTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATATTGTGGAC‑TGCCCCCCCCGGTAGACGATc                                                                                                                >  1:677260/1‑140 (MQ=255)

                     ttCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TG‑CCCCCCCGGTAGACGATCCTg                                                                                                             >  1:94844/1‑140 (MQ=255)

                       cGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGc                                                                                                            <  1:913526/140‑1 (MQ=255)

                         aCGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGccc                                                                                                          >  2:1109361/1‑140 (MQ=255)

                         aCGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGccc                                                                                                          <  2:462482/140‑1 (MQ=255)

                         aCGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGccc                                                                                                          <  2:1330684/140‑1 (MQ=255)

                         aCGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGccc                                                                                                          <  1:1426063/140‑1 (MQ=255)

                                aTTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGtt                                                                                                   <  2:482453/140‑1 (MQ=255)

                                    tttCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAg                                                                                               <  1:1141704/140‑1 (MQ=255)

                                     ttCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTTCCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGt                                                                                              <  2:677260/140‑1 (MQ=255)

                                     ttCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGt                                                                                              <  2:1361291/140‑1 (MQ=255)

                                        ttGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGcc                                                                                           <  1:1109361/140‑1 (MQ=255)

                                         tGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGccc                                                                                          <  1:729988/140‑1 (MQ=255)

                                             tgctgGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTa                                                                                      >  2:1188726/1‑140 (MQ=255)

                                                       taatTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGc                                                                            >  2:1275827/1‑140 (MQ=255)

                                                               aaGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTa                                                                    >  1:1418037/1‑140 (MQ=255)

                                                                aGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTTC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAg                                                                   <  2:1018212/140‑1 (MQ=255)

                                                                aGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAg                                                                   <  1:1181307/140‑1 (MQ=255)

                                                                aGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAg                                                                   >  1:1085215/1‑140 (MQ=255)

                                                                  tATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCa                                                                 >  1:604596/1‑140 (MQ=255)

                                                                  tATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCa                                                                 <  2:92435/140‑1 (MQ=255)

                                                                    tCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCaaa                                                               >  1:831396/1‑140 (MQ=255)

                                                                    tCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCaaa                                                               >  2:599532/1‑140 (MQ=255)

                                                                    tCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAAt                                                              <  2:94844/140‑1 (MQ=255)

                                                                     cGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAAt                                                              <  1:1188726/140‑1 (MQ=255)

                                                                     cGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAAt                                                              <  1:1275827/140‑1 (MQ=255)

                                                                     cGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACAGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAAt                                                              <  2:1047526/140‑1 (MQ=255)

                                                                            ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATaca                                                       >  2:86206/1‑140 (MQ=255)

                                                                                 tGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCa                                                  <  2:1085215/140‑1 (MQ=255)

                                                                                  ggTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAg                                                 >  1:1147698/1‑140 (MQ=255)

                                                                                  ggTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAg                                                 >  1:150374/1‑140 (MQ=255)

                                                                                  ggTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAg                                                 <  1:599532/140‑1 (MQ=255)

                                                                                    tGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAggg                                               <  2:604596/140‑1 (MQ=255)

                                                                                    tGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACCTCAggg                                               <  1:86206/140‑1 (MQ=255)

                                                                                      agaTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAggggg                                             >  1:449190/1‑140 (MQ=255)

                                                                                       gaTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggggg                                            <  1:1034280/140‑1 (MQ=255)

                                                                                       gaTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggggg                                            <  2:1147698/140‑1 (MQ=255)

                                                                                         tCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGtat                                         <  2:563767/140‑1 (MQ=255)

                                                                                           aGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGtatt                                        >  2:665886/1‑140 (MQ=255)

                                                                                             gAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAggggggca                                          >  1:1271520/1‑134 (MQ=255)

                                                                                             gAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAggggggca                                          <  2:1271520/136‑3 (MQ=255)

                                                                                                  ggTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTag                                 >  2:743247/1‑140 (MQ=255)

                                                                                                   gTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTaga                                >  2:171848/1‑140 (MQ=255)

                                                                                                   gTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTaga                                >  1:90573/1‑140 (MQ=255)

                                                                                                   gTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGACCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAggg                                               <  1:649898/125‑1 (MQ=255)

                                                                                                   gTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGACCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAggg                                               >  2:649898/1‑125 (MQ=255)

                                                                                                     tACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGt                              >  1:936254/1‑140 (MQ=255)

                                                                                                       ccGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGttt                            >  2:346157/1‑140 (MQ=255)

                                                                                                       ccGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGttt                            >  1:1134567/1‑140 (MQ=255)

                                                                                                        cGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTc                           >  2:1017013/1‑140 (MQ=255)

                                                                                                         gAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTcc                          <  2:936254/140‑1 (MQ=255)

                                                                                                           aaCGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTcccc                        <  1:171848/140‑1 (MQ=255)

                                                                                                            aCGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCa                       >  1:1467904/1‑140 (MQ=255)

                                                                                                             cGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCat                      <  2:106944/140‑1 (MQ=255)

                                                                                                             cGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCat                      <  1:665886/140‑1 (MQ=255)

                                                                                                              gATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCata                     <  2:90573/140‑1 (MQ=255)

                                                                                                               aTGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCatat                    <  2:55461/140‑1 (MQ=255)

                                                                                                                tGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATg                   >  1:383240/1‑140 (MQ=255)

                                                                                                                 ggACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGt                  >  1:405668/1‑140 (MQ=255)

                                                                                                                 ggACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGt                  <  2:383240/140‑1 (MQ=255)

                                                                                                                   aCATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGttt                <  1:743247/140‑1 (MQ=255)

                                                                                                                        atcCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATg           >  1:544864/1‑140 (MQ=255)

                                                                                                                        atcCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATg           <  2:544864/140‑1 (MQ=255)

                                                                                                                        atcCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATg           >  2:1064496/1‑140 (MQ=255)

                                                                                                                        atcCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATg           <  2:1418037/140‑1 (MQ=255)

                                                                                                                                 tttGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTCTAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAgg  >  2:1294287/1‑140 (MQ=255)

TGGGCTCTCTGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGG  >  NC_000913/2765648‑2765915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A