Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F28 I1 R1	151	87.4	2971636	99.2%	2947862	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,150,701	G→A	intergenic (+155/+117)	yghA → / ← exbD	NADP(+)‑dependent aldehyde reductase/Ton complex subunit ExbD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,150,701	0	G	A	100.0%	202.7 / NA	59	intergenic (+155/+117)	yghA/exbD	NADP(+)‑dependent aldehyde reductase/Ton complex subunit ExbD
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (28/31); total (28/31)

GCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCG  >  NC_000913/3150568‑3150825

gCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTa                                                                                                                        >  2:657053/1‑140 (MQ=255)

 cAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTaa                                                                                                                       >  2:722223/1‑140 (MQ=255)

 cAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTaa                                                                                                                       >  1:1435605/1‑140 (MQ=255)

    ccGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGc                                                                                                                    <  1:537293/140‑1 (MQ=255)

         catcatTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGAt                                                                                                               >  1:779732/1‑140 (MQ=255)

            catTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGAt                                                                                                               >  1:1320829/1‑137 (MQ=255)

            catTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGAt                                                                                                               <  2:1320829/137‑1 (MQ=255)

            catTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGATGcg                                                                                                            <  2:779732/140‑1 (MQ=255)

                 tgAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGATGCGTCGTg                                                                                                       <  1:1403721/140‑1 (MQ=255)

                 tgAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGATGCGTCGTg                                                                                                       <  2:1435605/140‑1 (MQ=255)

                  gAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGATGCGTCGTGa                                                                                                      >  2:346516/1‑140 (MQ=255)

                   aaTTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGa                                                                                                                >  2:896451/1‑129 (MQ=255)

                   aaTTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGa                                                                                                                <  1:896451/129‑1 (MQ=255)

                       cACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGATGCGTCGTGAACGcc                                                                                                 <  2:889474/140‑1 (MQ=255)

                        aCTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGATGCGTCGTGAACGCCt                                                                                                >  2:321157/1‑140 (MQ=255)

                        aCTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGGAGCGCATCAGGCATTGAACACCTAATGCCGGATGCGTCGTGAACGCCt                                                                                                >  2:261492/1‑140 (MQ=255)

                         ctctTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGATGCGTCGTGAACGCCtt                                                                                               >  2:221305/1‑140 (MQ=255)

                         ctctTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGATGCGTCGTGAACGCCtt                                                                                               >  2:1065021/1‑140 (MQ=255)

                            ttACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGATGCGTCGTGAACGCCTTATc                                                                                            <  1:321157/140‑1 (MQ=255)

                            ttACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGATGCGTCGTGAACGCCTTATc                                                                                            <  1:386023/140‑1 (MQ=255)

                            ttACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGATGCGTCGTGAACGCCTTATc                                                                                            <  1:221305/140‑1 (MQ=255)

                            ttACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGATGCGTCGTGAACGCCTTATc                                                                                            <  1:722223/140‑1 (MQ=255)

                                   aTGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTa                                                                                     >  2:1092805/1‑140 (MQ=255)

                                    tgtgGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTAc                                                                                    >  2:182564/1‑140 (MQ=255)

                                    tgtgGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTAc                                                                                    >  1:1468160/1‑140 (MQ=255)

                                    tgtgGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTAc                                                                                    >  1:131684/1‑140 (MQ=255)

                                      tgGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACaa                                                                                  >  2:48086/1‑140 (MQ=255)

                                        gCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAAc                                                                                >  1:1398105/1‑140 (MQ=255)

                                        gCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAAc                                                                                >  1:1238739/1‑140 (MQ=255)

                                        gCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAAc                                                                                >  1:1164002/1‑140 (MQ=255)

                                            aaaCGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCAc                                                                            <  1:1065021/140‑1 (MQ=255)

                                            aaaCGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCAc                                                                            <  1:1189989/140‑1 (MQ=255)

                                            aaaCGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCAc                                                                            <  1:346516/140‑1 (MQ=255)

                                             aaCGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACg                                                                           <  1:48086/140‑1 (MQ=255)

                                             aaCGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACg                                                                           >  1:221906/1‑140 (MQ=255)

                                                     aTCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCa                                                                   >  2:778937/1‑140 (MQ=255)

                                                          ccTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGTCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATg                                                              <  1:261492/140‑1 (MQ=255)

                                                          ccTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATg                                                              >  2:49871/1‑140 (MQ=255)

                                                               aaaCGCATGAAGATTCAACATGTTGCTATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCa                                                         <  1:246758/140‑1 (MQ=255)

                                                               aaaCGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCa                                                         <  1:778937/140‑1 (MQ=255)

                                                               aaaCGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCa                                                         <  2:131684/140‑1 (MQ=255)

                                                                  cGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATa                                                      <  2:1238739/140‑1 (MQ=255)

                                                                  cGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATa                                                      <  2:1398105/140‑1 (MQ=255)

                                                                    cATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGa                                                    <  2:1468160/140‑1 (MQ=255)

                                                                     aTGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGAt                                                   >  1:429228/1‑140 (MQ=255)

                                                                         aGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTg                                               <  1:657053/140‑1 (MQ=255)

                                                                         aGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTg                                               <  2:429228/140‑1 (MQ=255)

                                                                           aTTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTa                                             <  2:1164002/140‑1 (MQ=255)

                                                                                  atgatgCAATAGAAATTGCAGGTCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGGTGGGTCGTGAACGCCTTTTCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCtga                                      <  2:1454188/140‑1 (MQ=25)

                                                                                    gTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCtgatg                                    >  1:546214/1‑140 (MQ=255)

                                                                                      tGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAg                                  <  2:546214/140‑1 (MQ=255)

                                                                                           tAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAg                             <  1:1130327/140‑1 (MQ=255)

                                                                                                  tGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCa                      >  2:950046/1‑140 (MQ=255)

                                                                                                    tAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAgg                    <  2:532373/140‑1 (MQ=255)

                                                                                                    tAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAgg                    <  2:1077453/140‑1 (MQ=255)

                                                                                                        ccTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAAt                >  2:162622/1‑140 (MQ=255)

                                                                                                        ccTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAAt                >  1:532373/1‑140 (MQ=255)

                                                                                                            aTAAGCGTAGCGCATCAGGCATTGAACACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCta            <  1:1092805/140‑1 (MQ=255)

                                                                                                                      cgcATCAGGCATTGAACACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCaa                 >  2:113592/1‑125 (MQ=255)

                                                                                                                      cgcATCAGGCATTGAACACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCaa                 <  1:113592/125‑1 (MQ=255)

                                                                                                                      cgcATCAGGCATTGAACACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCg  >  2:1120682/1‑140 (MQ=255)

GCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCG  >  NC_000913/3150568‑3150825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A