Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,158,553	(A)_5→6	coding (904/927 nt)	yqhG →	DUF3828 domain‑containing protein YqhG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,158,548	1	.	A	100.0%	163.1 / NA	47	A300E (GCA→GAA)	yqhG	DUF3828 domain‑containing protein YqhG
Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (23/24); total (23/24)

ATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGC‑AAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGAACACTATATGAAAACGATTTTCACCGTGGGAGCTGTTGTTCTGGCAACCTGCTTGCTCAGTG  >  NC_000913/3158415‑3158681

aTTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGC‑aaaaaa                                                                                                                                 >  1:345260/1‑139 (MQ=255)

  tCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGC‑aaaaaact                                                                                                                               <  1:337520/140‑4 (MQ=255)

       ttCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAAACTgccgc                                                                                                                           <  2:861988/140‑1 (MQ=255)

       ttCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAAACTgccgc                                                                                                                           <  2:17819/140‑1 (MQ=255)

       ttCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAAACTgccgc                                                                                                                           <  2:824135/140‑1 (MQ=255)

         cGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAAACTGCCGCCa                                                                                                                         >  2:559014/1‑140 (MQ=255)

            tttACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTATAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAAACTGCCGCCAGAt                                                                                                                      >  2:609311/1‑140 (MQ=255)

                   tGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAAACTGCCGCCAGATTAAAGCa                                                                                                               >  1:773062/1‑140 (MQ=255)

                     aCCAATGGCAACAACGAAGAGCAAGAACTGGTTTTAAAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAAACTGCCGCCAGATTAAAGCAAt                                                                                                             <  1:590398/140‑1 (MQ=255)

                      ccAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAAACTGCCGCCAGATTAAAGCAATg                                                                                                            >  2:557650/1‑140 (MQ=255)

                             caacaaCGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAAACTGCCGCCAGATTAAAGCAATGAGCTGaa                                                                                                     <  1:559014/140‑1 (MQ=255)

                             caacaaCGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAAACTGCCGCCAGATTAAAGCAATGAGCTGaa                                                                                                     >  2:177746/1‑140 (MQ=255)

                             caacaaCGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAAACTGCCGCCAGATTAAAGCAATGAGCTGaa                                                                                                     <  1:834011/140‑1 (MQ=255)

                               acaacGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAAACTGCCGCCAGATTAAAGCa                                                                                                               >  1:209993/1‑128 (MQ=255)

                               acaacGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAAACTGCCGCCAGATTAAAGCa                                                                                                               <  2:209993/128‑1 (MQ=255)

                               acaacGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAAtt                                                                                                   >  2:163645/1‑140 (MQ=255)

                               acaacGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAAtt                                                                                                   <  2:83006/140‑1 (MQ=255)

                                   cGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAAt                                                                                               <  1:177746/140‑1 (MQ=255)

                                    gAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATa                                                                                              >  2:574449/1‑140 (MQ=255)

                                              aaCTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCGGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGcc                                                                                    >  2:203625/1‑140 (MQ=255)

                                                    ttttACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACa                                                                              <  2:305730/140‑1 (MQ=255)

                                                      ttACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACaat                                                                            <  1:557650/140‑1 (MQ=255)

                                                           gcggcgCGAAGGCAAGTGGGAAATCGCTGATTTTATCCGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACaataaata                                                                       <  1:574449/140‑1 (MQ=255)

                                                             ggcgCGAAGGCAAGTGGGAAATCGCTGATTTTATCCGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAAtaaataaa                                                                     >  1:187403/1‑140 (MQ=255)

                                                              gcgcGAAGGCAAGTGGGAAATCGCTGATTTTATCCGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATaaaa                                                                    >  1:664363/1‑140 (MQ=255)

                                                                gcgAAGGCAAGTGGGAAATCGCTGATTTTATCCGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAgg                                                                  <  2:637572/140‑1 (MQ=255)

                                                                 cgAAGGCAAGTGGGAAATCGCTGATTTTATCCGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAggg                                                                 >  1:706185/1‑140 (MQ=255)

                                                                   aaGGCAAGTGGGAAATCGCTGATTTTATCCGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGaa                                                               <  1:78086/140‑1 (MQ=255)

                                                                   aaGGCAAGTGGGAAATCGCTGATTTTATCCGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGaa                                                               <  2:250912/140‑1 (MQ=255)

                                                                   aaGGCAAGTGGGAAATCGATGATTTTATCCGCCCGACCAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAAATAAATAACAATTAGCCGGAACAATAAATAAAAGGGaa                                                               <  2:215872/140‑1 (MQ=255)

                                                                     ggCAAGTGGGAAATCGCTGATTTTATCCGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGAaca                                                             <  1:592648/140‑1 (MQ=255)

                                                                     ggCAAGTGGGAAATCGCTGATTTTATCCGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGAaca                                                             <  2:664363/140‑1 (MQ=255)

                                                                         aGTGGGAAATCGCTGATTTTATCCGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGAACACtat                                                         >  2:694756/1‑140 (MQ=255)

                                                                               aaaTCGCTGATTTTATCCGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGAACACTATATGaaa                                                   <  2:773062/140‑1 (MQ=255)

                                                                                aaTCGCTGATTTTATCCGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGAACACTATATGaaaa                                                  >  1:232369/1‑140 (MQ=255)

                                                                                 aTCGCTGATTTTATCCGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGAACACTATATGAAAAc                                                 <  1:609311/140‑1 (MQ=255)

                                                                                     cTGATTTTATCCGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGAACACTATATGAAAACGAtt                                             >  1:629820/1‑140 (MQ=255)

                                                                                     cTGATTTTATCCGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGAACACTATATGAAAACGAtt                                             >  1:774838/1‑140 (MQ=255)

                                                                                                   ccGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGAACACTATATGAAAACGAtttt                                           >  1:417557/1‑128 (MQ=255)

                                                                                                   ccGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGAACACTATATGAAAACGAtttt                                           <  2:417557/128‑1 (MQ=255)

                                                                                                   ccGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGAACACTATATGAAAACGATTTTCACCGTGGGAGc                               >  1:55341/1‑140 (MQ=255)

                                                                                                     gAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGAACACTATATGAAAACGATTTTCACCGTGGGAGCtg                             <  1:203625/140‑1 (MQ=255)

                                                                                                                 ccTACTTAAGCAGATTGAGGCAAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGAACACTATATGAAAACGATTTTCACCGTGGGAGCTGTTGTTCTGGCaa                 <  2:706185/140‑1 (MQ=255)

                                                                                                                 ccTACTTAAGCAGATTGAGGCAAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGAACACTATATGAAAACGATTTTCACCGTGGGAGCTGTTGTTCTGGCaa                 <  1:163645/140‑1 (MQ=255)

                                                                                                                          gCAGATTGAGGCAAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGAACACTATATGAAAACGATTTTCACCGTGGGAGCTGTTGTTCTGGCAACCtgcttgc        >  2:702843/1‑140 (MQ=255)

                                                                                                                           cAGATTGAGGCAAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGAACACTATATGAAAACGATTTTCACCGTGGGAGCTGTTGTTCTGGCAACCtgcttgct       <  1:702843/140‑1 (MQ=255)

                                                                                                                             gATTGAGGCAAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGAACACTATATGAAAACGATTTTCACCGTGGGAGCTGTTGTTCTGGCAACCTGCTTGCTCa     >  2:381080/1‑140 (MQ=255)

                                                                                                                              aTTGAGGCAAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGAACACTATATGAAAACGATTTTCACCGTGGGAGCTGTTGTTCTGGCAACCTGCTTGCTCAg    >  2:599915/1‑140 (MQ=255)

                                                                                                                               ttGAGGCAAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGAACACTATATGAAAACGATTTTCACCGTGGGAGCTGTTGTTCTGGCAACCTGCTTGCTCAGt   >  2:323244/1‑140 (MQ=255)

                                                                                                                               ttGAGGCAAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGAACACTATATGAAAACGATTTTCACCGTGGGAGCTGTTGTTCTGGCAACCTGCTTGCTCAGt   >  2:833184/1‑140 (MQ=255)

                                                                                                                                tGAGGCAAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGAACACTATATGAAAACGATTTTCACCGTGGGAGCTGTTGTTCTGGCAACCTGCTTGCTCAGTg  >  1:591602/1‑140 (MQ=255)

ATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGC‑AAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGAACACTATATGAAAACGATTTTCACCGTGGGAGCTGTTGTTCTGGCAACCTGCTTGCTCAGTG  >  NC_000913/3158415‑3158681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A