Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F28 I1 R1	151	87.4	2971636	99.2%	2947862	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,359,183	T→C	*1487Q (TAA→CAA)	gltB →	glutamate synthase subunit GltB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,359,183	0	T	C	100.0%	258.5 / NA	75	*1487Q (TAA→CAA)	gltB	glutamate synthase subunit GltB
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (39/36); total (39/36)

AGCGCGGTGAAGAGATTCTGGCGAACTGGTCAACCTTCGCCACTAAATTTGCGCTGGTTAAACCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAGTTTGTTGAAATTTACGAGCCGTTTTCCGAAGGCCAGGCC  >  NC_000913/3359046‑3359317

aGCGCGGTGAAGAGATTCTGGCGAACTGGTCAACCTTCGCCACTAAATTTGCGCTGGTTAAACCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCaa                                                                                                                                      >  2:992513/1‑140 (MQ=255)

 gcgcGGTGAAGAGATTCTGGCGAACTGGTCAACCTTCGCCACTAAATTTGCGCTGGTTAAACCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAg                                                                                                                                     <  1:1138811/140‑1 (MQ=255)

    cgGTGAAGAGATTCTGGCGAACTGGTCAACCTTCGCCACTAAATTTGCGCTGGTTAAACCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAgggg                                                                                                                                  >  2:743109/1‑140 (MQ=255)

    cgGTGAAGAGATTCTGGCGAACTGGTCAACCTTCGCCACTAAATTTGCGCTGGTTAAACCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAgggg                                                                                                                                  >  1:586893/1‑140 (MQ=255)

    cgGTGAAGAGATTCTGGCGAACTGGTCAACCTTCGCCACTAAATTTGCGCTGGTTAAACCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAgggg                                                                                                                                  <  1:483250/140‑1 (MQ=255)

      gTGAAGAGATTCTGGCGAACTGGTCAACCTTCGCCACTAAATTTGCGCTGGTTAAACCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTa                                                                                                                                <  2:689882/140‑1 (MQ=255)

        gAAGAGATTCTGGCGAACTGGTCAACCTTCGCCACTAAATTTGCGCTGGTTAAACCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGc                                                                                                                              <  2:194341/140‑1 (MQ=255)

            agaTTCTAGCGAACTGGTCAACCTTCGCCACTAAATTTGCGCTGGTTAAACCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGcaaca                                                                                                                          <  1:205731/140‑1 (MQ=255)

             gaTTCTGGCGAACTGGTCAACCTTCGCCACTAAATTTGCGCTGGTTAAACCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGcaacaa                                                                                                                         <  1:101782/140‑1 (MQ=255)

               ttCTGGCGAACTGGTCAACCTTCGCCACTAAATTTGCGCTGGTTAAACCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATg                                                                                                                       <  2:67478/140‑1 (MQ=255)

                 cTGGCGAACTGGTCAACCTTCGCCACTAAATTTGCGCTGGTTAAACCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAg                                                                                                                     >  2:698490/1‑140 (MQ=255)

                   ggCGAACTGGTCAACCTTCGCCACTAAATTTGCGCTGGTTAAACCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGcaaca                                                                                                                          >  2:1265295/1‑133 (MQ=255)

                   ggCGAACTGGTCAACCTTCGCCACTAAATTTGCGCTGGTTAAACCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGcaaca                                                                                                                          <  1:1265295/133‑1 (MQ=255)

                    gCGAACTGGTCAACCTTCGCCACTAAATTTGCGCTGGTTAAACCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCa                                                                                                                  <  1:186731/140‑1 (MQ=255)

                      gAACTGGTCAACCTTCGCCACTAAATTTGCGCTGGTTAAACCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGa                                                                                                                <  2:1317311/140‑1 (MQ=255)

                      gAACTGGTCAACCTTCGCCACTAAATTTGCGCTGGTTAAACCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGa                                                                                                                <  1:328598/140‑1 (MQ=255)

                      gAACTGGTCAACCTTCGCCACTAAATTTGCGCTGGTTAAACCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCCTAGTCGTAGCGCAGCTGAATTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGa                                                                                                                <  2:1327609/140‑1 (MQ=255)

                        aCTGGTCAACCTTCGCCACTAAATTTGCGCTGGTTAAACCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAAt                                                                                                              >  2:1187842/1‑140 (MQ=255)

                            gTCAACCTTCGCCACTAAATTTGCGCTGGTTAAACCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGttt                                                                                                          >  2:338182/1‑140 (MQ=255)

                            gTCAACCTTCGCCACTAAATTTGCGCTGGTTAAACCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGttt                                                                                                          >  1:1025714/1‑140 (MQ=255)

                              cAACCTTCGCCACTAAATTTGCGCTGGTTAAACCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTAt                                                                                                        <  2:1375051/140‑1 (MQ=255)

                              cAACCTTCGCCACTAAATTTGCGCTGGTTAAACCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTAt                                                                                                        >  1:818609/1‑140 (MQ=255)

                                      gCCACTAAATTTGCGCTGGTTAAACCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGa                                                                                                                >  2:350973/1‑124 (MQ=255)

                                      gCCACTAAATTTGCGCTGGTTAAACCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGa                                                                                                                <  1:350973/124‑1 (MQ=255)

                                          cTAAATTTGCGCTGGTTAAACCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGAc                                                                                            <  1:1187842/140‑1 (MQ=255)

                                          cTAAATTTGCGCTGGTTAAACCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGAc                                                                                            <  1:992513/140‑1 (MQ=255)

                                           tAAATTTGCGCTGGTTAAACCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTa                                                                                                 <  1:384121/134‑1 (MQ=255)

                                           tAAATTTGCGCTGGTTAAACCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTa                                                                                                 >  2:384121/1‑134 (MQ=255)

                                           tAAATTTGCGCTGGTTAAACCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGAcc                                                                                           >  1:984242/1‑140 (MQ=255)

                                           tAAATTTGCGCTGGTTAAACCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGAcc                                                                                           >  1:190951/1‑140 (MQ=255)

                                           tAAATTTGCGCTGGTTAAACCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGAcc                                                                                           >  1:1184249/1‑140 (MQ=255)

                                               tttGCGCTGGTTAAACCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCa                                                                                       <  1:946751/140‑1 (MQ=255)

                                                   cgcTGGTTAAACCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAgcgc                                                                                   <  1:743109/140‑1 (MQ=255)

                                                    gcTGGTTAAACCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGcgcg                                                                                  <  2:1025714/140‑1 (MQ=255)

                                                                  aGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTGAt                                                                             <  2:3096/131‑1 (MQ=255)

                                                                  aGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTGAt                                                                             >  1:3096/1‑131 (MQ=255)

                                                                  aGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTGATCCGCCAAAg                                                                    >  1:113690/1‑140 (MQ=255)

                                                                  aGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTGATCCGCCAAAg                                                                    >  2:1145805/1‑140 (MQ=255)

                                                                  aGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTGATCCGCCAAAg                                                                    >  2:1108685/1‑140 (MQ=255)

                                                                   gTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTGATCCGCCAAAGa                                                                   >  1:141302/1‑140 (MQ=255)

                                                                      cAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTGATCCGCCAAAGAAAc                                                                <  2:1184249/140‑1 (MQ=255)

                                                                      cAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTGATCCGCCAAAGAAAc                                                                <  2:781582/140‑1 (MQ=255)

                                                                      cAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTGATCCGCCAAAGAAAc                                                                <  1:338182/140‑1 (MQ=255)

                                                                             gTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGaa                                                         >  1:1311584/1‑140 (MQ=255)

                                                                              tAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAg                                                        >  1:1084601/1‑140 (MQ=255)

                                                                              tAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAg                                                        >  1:502542/1‑140 (MQ=255)

                                                                                aaaGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCATGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGAt                                                      <  1:1482266/140‑1 (MQ=255)

                                                                                      ctgctgGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCaa                                                >  2:1423292/1‑140 (MQ=255)

                                                                                       tgctgGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCaaa                                               >  1:1006106/1‑140 (MQ=255)

                                                                                         ctgGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAGTGTTTATCAATTTATCGACCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAAt                                             >  2:1292322/1‑140 (MQ=255)

                                                                                         ctgGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAAt                                             <  2:1084601/140‑1 (MQ=255)

                                                                                              tCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGa                                          <  1:458366/138‑1 (MQ=255)

                                                                                              tCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGa                                          >  2:471349/1‑138 (MQ=255)

                                                                                              tCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGa                                          >  2:458366/1‑138 (MQ=255)

                                                                                              tCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGa                                          <  1:471349/138‑1 (MQ=255)

                                                                                              tCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAGt                                        <  2:141302/140‑1 (MQ=255)

                                                                                                   gTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAGTttgtt                                   >  1:460426/1‑140 (MQ=255)

                                                                                                     aGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGACCCGCAAAATTGAGTTTGTTGa                                 <  1:1108685/140‑1 (MQ=255)

                                                                                                      gTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAGTTTGTTGaa                                >  2:1197847/1‑140 (MQ=255)

                                                                                                          tAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAGTTTGTTGAAAttt                            >  2:1019177/1‑140 (MQ=255)

                                                                                                               cAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAGTTTGTTGAAATTTACGAg                       >  1:1446520/1‑140 (MQ=255)

                                                                                                               cAGCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAGTTTGTTGAAATTTACGAg                       <  2:818609/140‑1 (MQ=255)

                                                                                                                 gCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAGTTTGTTGAAATTTACGAGcc                     >  1:831260/1‑140 (MQ=255)

                                                                                                                 gCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAGTTTGTTGAAATTTACGAGcc                     >  1:1435881/1‑140 (MQ=255)

                                                                                                                 gCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAGTTTGTTGAAATTTACGAGcc                     >  2:794942/1‑140 (MQ=255)

                                                                                                                 gCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAGTTTGTTGAAATTTACGAGcc                     >  2:921797/1‑140 (MQ=255)

                                                                                                                 gCTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAGTTTGTTGAAATTTACGAGcc                     >  1:1244845/1‑140 (MQ=255)

                                                                                                                  cTGAGTTGCGCGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAGTTTGTTGAAATTTACGAGCCg                    >  2:328947/1‑140 (MQ=255)

                                                                                                                         gcgcGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAGTTTGTTGAAATTTACGAGCCGTTTTCCg             <  1:921797/140‑1 (MQ=255)

                                                                                                                         gcgcGTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAGTTTGTTGAAATTTACGAGCCGTTTTCCg             <  2:1244845/140‑1 (MQ=255)

                                                                                                                          cgcgTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAGTTTGTTGAAATTTACGAGCCGTTTTCCGa            >  2:1157316/1‑140 (MQ=255)

                                                                                                                            cgTGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAGTTTGTTGAAATTTACGAGCCGTTTTCCGAag          >  1:804379/1‑140 (MQ=255)

                                                                                                                              tGCAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAGTTTGTTGAAATTTACGAGCCGTTTTCCGAaggc        <  2:1006106/140‑1 (MQ=255)

                                                                                                                                cAGGCGCAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGTGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAGTTTGTTGAAATTTACGAGCCGTTTTCCGAaggcca      <  2:831260/140‑1 (MQ=255)

                                                                                                                                    cgcAGCAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAGTTTGTTGAAATTTACGAGCCGTTTTCCGAaggccaggcc  <  1:1019177/140‑1 (MQ=255)

AGCGCGGTGAAGAGATTCTGGCGAACTGGTCAACCTTCGCCACTAAATTTGCGCTGGTTAAACCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAGTTTGTTGAAATTTACGAGCCGTTTTCCGAAGGCCAGGCC  >  NC_000913/3359046‑3359317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A