Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F28 I1 R1 151 87.4 2971636 99.2% 2947862 138.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,432,994 (C)6→7 coding (567/1125 nt) smf ← protein Smf

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,432,9881.C97.0% 338.8 / 2.2 100coding (573/1125 nt)smfprotein Smf
Reads supporting (aligned to +/- strand):  ref base . (1/2);  new base C (42/55);  total (43/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

Only 100 of 113 total aligned reads displayed.

TGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑CCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCTTT  >  NC_000913/3432850‑3433101
                                                                                                                                           |                                                                                                                  
tGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAgcg‑c                                                                                                                   >  1:843091/1‑140 (MQ=255)
tGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAgcg‑c                                                                                                                   >  1:193336/1‑140 (MQ=255)
 gCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑cc                                                                                                                  >  2:1261744/1‑140 (MQ=255)
   tGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑cccc                                                                                                                <  2:1023169/140‑1 (MQ=255)
    gTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑cccc‑c                                                                                                              <  1:622107/140‑1 (MQ=255)
     tCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑cccc‑cc                                                                                                             <  2:172301/140‑1 (MQ=255)
     tCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑cccc‑cc                                                                                                             <  2:680937/140‑1 (MQ=255)
      cACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑cccc‑ccc                                                                                                            <  2:1163267/140‑2 (MQ=255)
      cACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑cccc‑ccc                                                                                                            <  2:416708/140‑2 (MQ=255)
       aCCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑cccc‑ccct                                                                                                           >  1:709410/1‑138 (MQ=255)
        ccAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑cccc‑ccctg                                                                                                          <  2:53375/140‑4 (MQ=255)
        ccAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑cccc‑ccctg                                                                                                          <  2:709410/140‑4 (MQ=255)
           gCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTc                                                                                                        >  2:1451514/1‑140 (MQ=255)
              aaCCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAg                                                                                                     >  2:1025832/1‑140 (MQ=255)
              aaCCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAg                                                                                                     >  2:3612/1‑140 (MQ=255)
               aCCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGc                                                                                                    >  1:1066628/1‑140 (MQ=255)
                ccACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCa                                                                                                   <  2:703158/140‑1 (MQ=255)
                    tACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACt                                                                                               <  2:843091/140‑1 (MQ=255)
                        cAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCa                                                                                           <  1:372456/140‑1 (MQ=255)
                           aGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGcc                                                                                        >  1:1157826/1‑140 (MQ=255)
                           aGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGcc                                                                                        >  2:1036189/1‑140 (MQ=255)
                                cTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCGGTTCAAGCAGACTGGCAGCCAGTCg                                                                                   >  1:91496/1‑140 (MQ=255)
                                     accaccaGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCAt                                                                              <  2:263332/140‑1 (MQ=255)
                                     accaccaGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCAt                                                                              <  1:815433/140‑1 (MQ=255)
                                     accaccaGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCAt                                                                              >  2:553962/1‑140 (MQ=255)
                                       caccaGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGa                                                                            <  2:514566/140‑1 (MQ=255)
                                         ccaGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGAcg                                                                          <  1:279647/140‑1 (MQ=255)
                                          caGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGAcgg                                                                         <  1:1025832/140‑1 (MQ=255)
                                          caGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGAcgg                                                                         <  1:3612/140‑1 (MQ=255)
                                           aGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGAcggc                                                                        <  1:524320/140‑1 (MQ=255)
                                            gTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGAcggcg                                                                       <  2:745855/140‑1 (MQ=255)
                                               cacCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCgggg                                                                    <  2:712018/140‑1 (MQ=255)
                                                acCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGa                                                                   <  1:1098649/140‑1 (MQ=255)
                                                 ccTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGAt                                                                  <  2:1066628/140‑1 (MQ=255)
                                                   tttACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGa                                                                <  1:1329700/140‑1 (MQ=255)
                                                   tttACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGa                                                                <  1:159149/140‑1 (MQ=255)
                                                    ttACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGaa                                                               >  2:1255919/1‑140 (MQ=255)
                                                    ttACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGaa                                                               <  2:1281754/140‑1 (MQ=255)
                                                    ttACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGaa                                                               >  2:949258/1‑140 (MQ=255)
                                                    ttACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGaa                                                               <  1:286493/140‑1 (MQ=255)
                                                    ttACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGaa                                                               <  1:1286469/140‑1 (MQ=255)
                                                    ttACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGaa                                                               <  1:553962/140‑1 (MQ=255)
                                                    ttACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGaa                                                               <  1:1451514/140‑1 (MQ=255)
                                                    ttACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGa                                                                >  1:1315981/1‑139 (MQ=255)
                                                    ttACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAACTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAACCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGa                                                                <  2:1315981/139‑1 (MQ=255)
                                                     tACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAAt                                                              >  1:36990/1‑140 (MQ=255)
                                                       cTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGaa                                                               <  1:662427/137‑1 (MQ=255)
                                                       cTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGaa                                                               >  2:662427/1‑137 (MQ=255)
                                                       cTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATgg                                                            >  1:1370130/1‑140 (MQ=255)
                                                            aCCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGAt                                                                  >  2:771466/1‑129 (MQ=255)
                                                            aCCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGAt                                                                  <  1:771466/129‑1 (MQ=255)
                                                            aCCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAAt                                                              <  2:517899/133‑1 (MQ=255)
                                                            aCCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAAt                                                              >  1:517899/1‑133 (MQ=255)
                                                            aCCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTa                                                       >  2:370502/1‑140 (MQ=255)
                                                            aCCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTa                                                       >  1:781563/1‑140 (MQ=255)
                                                            aCCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑CCCC‑CCCGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGaa                                                               >  2:281009/1‑131 (MQ=255)
                                                            aCCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑CCCC‑CCCGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGaa                                                               <  1:281009/131‑1 (MQ=255)
                                                             ccACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTaa                                                      <  2:1157826/140‑1 (MQ=255)
                                                             ccACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTaa                                                      <  1:375931/140‑1 (MQ=255)
                                                                cTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTc                                                   >  2:1385325/1‑140 (MQ=255)
                                                                cTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTc                                                   >  1:775113/1‑140 (MQ=255)
                                                                 tGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTcc                                                  >  1:1060226/1‑140 (MQ=255)
                                                                 tGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTcc                                                  >  1:636902/1‑140 (MQ=255)
                                                                 tGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTc                                                   <  2:202229/139‑1 (MQ=255)
                                                                 tGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTc                                                   >  1:202229/1‑139 (MQ=255)
                                                                 tGATAATGCGATTTCTTCGTAGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAAt                                                              >  1:1483047/1‑128 (MQ=255)
                                                                 tGATAATGCGATTTCTTCGTAGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAAt                                                              <  2:1483047/128‑1 (MQ=255)
                                                                  gATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCa                                                 <  2:1060226/140‑1 (MQ=255)
                                                                  gATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCa                                                 <  1:993687/140‑1 (MQ=255)
                                                                  gATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑CCCCGCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCa                                                 <  2:193336/140‑1 (MQ=255)
                                                                     aaTGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTc                                              <  2:775113/140‑1 (MQ=255)
                                                                     aaTGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTc                                              <  1:1341141/140‑1 (MQ=255)
                                                                      aTGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAAt                                                              >  2:702461/1‑123 (MQ=255)
                                                                      aTGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTcc                                             >  2:181293/1‑140 (MQ=255)
                                                                       tGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTccc                                            <  1:954480/140‑1 (MQ=255)
                                                                        gCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGGATTAAGTCCATTccc                                            >  2:285631/1‑139 (MQ=255)
                                                                             ttcttcGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCaa                                         >  2:808829/1‑137 (MQ=255)
                                                                             ttcttcGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCaa                                         <  1:1392561/137‑1 (MQ=255)
                                                                             ttcttcGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCaa                                         <  1:808829/137‑1 (MQ=255)
                                                                             ttcttcGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCaa                                         >  2:1392561/1‑137 (MQ=255)
                                                                                  cGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTccc                                            >  1:1295351/1‑129 (MQ=255)
                                                                                  cGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCaa                                 >  1:460319/1‑140 (MQ=255)
                                                                                  cGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGGATTAAGTCCATTCCCCAATACAGCaa                                 >  1:642491/1‑140 (MQ=255)
                                                                                       gAAAGTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTg                            <  2:1370130/140‑1 (MQ=255)
                                                                                             gTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCa                      <  2:636902/140‑1 (MQ=255)
                                                                                              tAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCAt                     <  2:1105986/140‑1 (MQ=255)
                                                                                                aGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCAttt                   <  2:742329/140‑1 (MQ=255)
                                                                                                aGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCAttt                   <  1:699953/140‑1 (MQ=255)
                                                                                                aGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCAttt                   <  1:1036189/140‑1 (MQ=255)
                                                                                                  cAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACg                         <  2:491855/132‑1 (MQ=255)
                                                                                                  cAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACg                         >  1:491855/1‑132 (MQ=255)
                                                                                                     ggggTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTg              <  1:15964/140‑1 (MQ=255)
                                                                                                     ggggTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTg              <  1:1075049/140‑1 (MQ=255)
                                                                                                     ggggTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTg              >  2:15964/1‑140 (MQ=255)
                                                                                                     ggggTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTg              >  2:1075049/1‑140 (MQ=255)
                                                                                                      gggTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGt             >  1:1106201/1‑140 (MQ=255)
                                                                                                       ggTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTa            <  2:36990/140‑1 (MQ=255)
                                                                                                          ggAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAgg         <  2:1453090/140‑1 (MQ=255)
                                                                                                            aaCATCGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGgct       <  1:161173/140‑1 (MQ=255)
                                                                                                                 cGAGGGGAAATTCCGAGACGAGAGCGCCCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCttt  >  1:708194/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                  
TGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑CCCC‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCTTT  >  NC_000913/3432850‑3433101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A