Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	260	73.5	2514320	99.3%	2496719	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,437,397	C→T	H294Y (CAT→TAT)	trkA →	NAD‑binding component of Trk potassium transporters

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,437,397	0	C	T	100.0%	226.6 / NA	64	H294Y (CAT→TAT)	trkA	NAD‑binding component of Trk potassium transporters
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (29/35); total (29/35)

TCGTCTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGT  >  NC_000913/3437264‑3437533

tcgtcTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAATATATCg                                                                                                                                    >  2:722870/1‑140 (MQ=255)

tcgtcTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAATATATCg                                                                                                                                    >  2:231293/1‑140 (MQ=255)

 cgtcTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAATATATCGa                                                                                                                                   <  1:1125047/140‑1 (MQ=255)

 cgtcTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAATATATCGa                                                                                                                                   <  2:449048/140‑1 (MQ=255)

      ggAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAATATATCGATCAAg                                                                                                                              <  2:1158544/140‑1 (MQ=255)

           aaGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAATATATCGATCAAGTTGAt                                                                                                                         <  2:980373/140‑1 (MQ=255)

           aaGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAATATATCGATCAAGTTGAt                                                                                                                         <  2:627639/140‑1 (MQ=255)

             gATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAATATATCGATCAAGTTGATCt                                                                                                                       >  2:1172249/1‑140 (MQ=255)

                                      gTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGAc                                                                                              <  1:494371/140‑1 (MQ=255)

                                      gTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGAc                                                                                              <  2:1198403/140‑1 (MQ=255)

                                       tAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACg                                                                                             <  2:522947/140‑1 (MQ=255)

                                         aTCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAATATATCGATCAAGTTGATCTGATTATTGCCGTCACCAACGATGACGAg                                                                                           >  2:710386/1‑140 (MQ=255)

                                            agcagcGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGcc                                                                                        <  1:335917/140‑1 (MQ=255)

                                            agcagcGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGcc                                                                                        <  2:40219/140‑1 (MQ=255)

                                             gcagcGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCa                                                                                       <  1:1048311/140‑1 (MQ=255)

                                             gcagcGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCa                                                                                       <  1:769244/140‑1 (MQ=255)

                                             gcagcGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCa                                                                                       <  1:673769/140‑1 (MQ=255)

                                             gcagcGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCa                                                                                       <  1:524241/140‑1 (MQ=255)

                                                 cgcgcTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAatat                                                                                   >  2:585125/1‑140 (MQ=255)

                                                  gcgcTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGAc                                                                                              >  2:507026/1‑128 (MQ=255)

                                                  gcgcTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGAc                                                                                              <  1:507026/128‑1 (MQ=255)

                                                   cgcTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCa                                                                                 >  2:94895/1‑140 (MQ=255)

                                                           aaCTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCAt                                                                                <  1:469790/133‑1 (MQ=255)

                                                           aaCTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCAt                                                                                >  2:469790/1‑133 (MQ=255)

                                                               ggCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGc                                                                     <  1:792108/140‑1 (MQ=255)

                                                                gCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCt                                                                    >  2:120471/1‑140 (MQ=255)

                                                                gCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCt                                                                    >  2:69998/1‑140 (MQ=255)

                                                                    aaaaGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGcc                                                                <  1:585125/140‑1 (MQ=255)

                                                                     aaaGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCa                                                               >  1:249029/1‑140 (MQ=255)

                                                                           aCAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTa                                                         <  1:94895/140‑1 (MQ=255)

                                                                                 tACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTg                                                   >  1:914142/1‑140 (MQ=255)

                                                                                 tACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTg                                                   >  1:735546/1‑140 (MQ=255)

                                                                                 tACGATCGTC‑TTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGc                                                  >  1:799331/1‑140 (MQ=255)

                                                                                    gATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGa                                                >  2:865258/1‑140 (MQ=255)

                                                                                      tCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGaaa                                              <  1:825351/140‑1 (MQ=255)

                                                                                         tCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGt                                                    >  2:82198/1‑131 (MQ=255)

                                                                                         tCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGt                                                    <  1:82198/131‑1 (MQ=255)

                                                                                           ttttttGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGt                                         <  1:959048/140‑1 (MQ=255)

                                                                                           ttttttGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGt                                         >  1:824828/1‑140 (MQ=255)

                                                                                             ttttGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGa                                       <  2:799331/140‑1 (MQ=255)

                                                                                              tttGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGAt                                      <  1:655860/140‑1 (MQ=255)

                                                                                                tGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGt                                         <  1:29296/135‑1 (MQ=255)

                                                                                                tGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGt                                         >  2:29296/1‑135 (MQ=255)

                                                                                                              aTCAAGAACTACTGGCCGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGt                      <  2:824828/140‑1 (MQ=255)

                                                                                                                  aGAACTACTGGCCGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTcgcg                  >  1:435138/1‑140 (MQ=255)

                                                                                                                   gAACTACTGGCCGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCgcgc                 <  1:897157/140‑1 (MQ=255)

                                                                                                                    aaCTACTGGCCGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAATAAGGTGATGGTATTGATCCAGCGTCGCGCt                >  2:769072/1‑140 (MQ=255)

                                                                                                                       tactGGCCGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTAt             >  1:503481/1‑140 (MQ=255)

                                                                                                                        actGGCCGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTAtg            >  2:88407/1‑140 (MQ=255)

                                                                                                                        actGGCCGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTAtg            >  2:1198031/1‑140 (MQ=255)

                                                                                                                        actGGCCGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTAtg            >  1:271629/1‑140 (MQ=255)

                                                                                                                        actGGCCGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTAtg            >  1:303593/1‑140 (MQ=255)

                                                                                                                        actGGCCGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTAtg            >  1:1168952/1‑140 (MQ=255)

                                                                                                                         ctGGCCGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTAtgt           <  1:769072/140‑1 (MQ=255)

                                                                                                                         ctGGCCGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTAtgt           <  2:914142/140‑1 (MQ=255)

                                                                                                                            gCCGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGa        <  2:260261/140‑1 (MQ=255)

                                                                                                                            gCCGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGGATTGATCCAGCGTCGCGCTTATGTGGa        >  1:260261/1‑140 (MQ=255)

                                                                                                                             ccGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGAt       >  2:870313/1‑140 (MQ=255)

                                                                                                                             ccGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGAGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGAt       <  2:278781/140‑1 (MQ=255)

                                                                                                                              cGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATc      <  1:865258/140‑1 (MQ=255)

                                                                                                                              cGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATc      <  1:862856/140‑1 (MQ=255)

                                                                                                                              cGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATc      <  1:205654/140‑1 (MQ=255)

                                                                                                                              cGAAGAATATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATc      <  2:249029/140‑1 (MQ=255)

                                                                                                                                 agaaTATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTgg   >  1:857630/1‑140 (MQ=255)

                                                                                                                                  gaaTATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGt  <  1:69998/140‑1 (MQ=255)

TCGTCTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGT  >  NC_000913/3437264‑3437533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A