Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F28 I1 R1 151 87.4 2971636 99.2% 2947862 138.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,904,456 T→C Y381C (TAC→TGC)  bglF ← beta‑glucoside specific PTS enzyme II/BglG kinase/BglG phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,904,4560TC100.0% 327.2 / NA 93Y381C (TAC→TGC) bglFbeta‑glucoside specific PTS enzyme II/BglG kinase/BglG phosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (55/38);  total (55/38)

TGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATCGCGTTCGCAGAGGAAGACGCCGAGCGCCGCCCCGACCTG  >  NC_000913/3904319‑3904592
                                                                                                                                         |                                                                                                                                        
tGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCAc                                                                                                                                        >  1:1039943/1‑140 (MQ=255)
tGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCAc                                                                                                                                        >  2:1428649/1‑140 (MQ=255)
tGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCAc                                                                                                                                        >  2:445557/1‑140 (MQ=255)
tGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCAc                                                                                                                                        >  2:1273807/1‑140 (MQ=255)
        aTTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCgg                                                                                                                                <  2:525826/140‑1 (MQ=255)
        aTTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCgg                                                                                                                                <  2:1039943/140‑1 (MQ=255)
         ttCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGc                                                                                                                               <  1:460987/140‑1 (MQ=255)
          tCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCa                                                                                                                              >  2:805625/1‑140 (MQ=255)
              gTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGtt                                                                                                                          <  1:1323515/140‑1 (MQ=255)
                  aCGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACg                                                                                                                      <  2:1127606/140‑1 (MQ=255)
                   cGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGc                                                                                                                     >  2:1481886/1‑140 (MQ=255)
                     ggATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCa                                                                                                                   <  2:534676/140‑1 (MQ=255)
                     ggATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCa                                                                                                                   <  1:1481886/140‑1 (MQ=255)
                       aTGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCata                                                                                                                 <  2:688273/140‑1 (MQ=255)
                            ttGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCg                                                                                                            <  2:159304/140‑1 (MQ=255)
                                     ggTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGt                                                                                                       >  1:257522/1‑136 (MQ=255)
                                     ggTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGt                                                                                                       <  2:257522/136‑1 (MQ=255)
                                     ggTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCgg                                                                                                   <  1:989324/140‑1 (MQ=255)
                                     ggTGAAAAGACTTTGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGGCCCACGGATACAGGCGATTAGAAAGGTGGACTTACGCGGCAGGTGGACGCCATATACCGCTGGGTcag                                                                                                   >  2:737074/1‑138 (MQ=255)
                                         aaaaTACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGAt                                                                                               <  1:1360009/140‑1 (MQ=255)
                                         aaaaTACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGAt                                                                                               <  1:805625/140‑1 (MQ=255)
                                         aaaaTACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGAt                                                                                               >  1:775825/1‑140 (MQ=255)
                                             tACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCa                                                                                           >  1:360478/1‑140 (MQ=255)
                                                ttGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAAc                                                                                        >  1:568013/1‑140 (MQ=255)
                                                 tGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATa                                                                                              <  1:1314993/133‑1 (MQ=255)
                                                 tGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATa                                                                                              >  2:1314993/1‑133 (MQ=255)
                                                 tGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACa                                                                                       >  2:1016263/1‑140 (MQ=255)
                                                   gCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGa                                                                                     >  2:1126196/1‑140 (MQ=255)
                                                           aaGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCaa                                                                             <  2:775825/140‑1 (MQ=255)
                                                           aaGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCaa                                                                             <  1:1273807/140‑1 (MQ=255)
                                                            aGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAAc                                                                            >  1:1441607/1‑140 (MQ=255)
                                                              gAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAAcgc                                                                          <  2:360478/140‑1 (MQ=255)
                                                                gTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAAcgccg                                                                        <  1:1126196/140‑1 (MQ=255)
                                                                 tAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCaa                                                                             <  2:369346/134‑1 (MQ=255)
                                                                 tAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCaa                                                                             >  1:369346/1‑134 (MQ=255)
                                                                 tAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAAcgccgc                                                                       >  2:312890/1‑140 (MQ=255)
                                                                 tAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAAcgccgc                                                                       >  2:414779/1‑140 (MQ=255)
                                                                   gACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTg                                                                     >  1:733966/1‑140 (MQ=255)
                                                                   gACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTg                                                                     >  1:1404789/1‑140 (MQ=255)
                                                                   gACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTg                                                                     >  1:1069348/1‑140 (MQ=255)
                                                                     cTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGAt                                                                   <  1:578122/140‑1 (MQ=255)
                                                                        tCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGt                                                                                <  1:830643/124‑1 (MQ=255)
                                                                        tCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGt                                                                                >  2:830643/1‑124 (MQ=255)
                                                                        tCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCAGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATccc                                                                >  2:379877/1‑140 (MQ=255)
                                                                           tttGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGcc                                                             >  1:1233725/1‑140 (MQ=255)
                                                                            ttGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGcca                                                            <  2:1069348/140‑1 (MQ=255)
                                                                            ttGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGcca                                                            <  1:468865/140‑1 (MQ=255)
                                                                               cgcgTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGccacca                                                         >  1:829251/1‑140 (MQ=255)
                                                                               cgcgTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGccacca                                                         >  2:172501/1‑140 (MQ=255)
                                                                                 cgTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACt                                                       <  2:568013/140‑1 (MQ=255)
                                                                                              ggtggCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTgcgc                                            <  2:57296/138‑1 (MQ=255)
                                                                                              ggtggCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTgcgc                                            >  1:57296/1‑138 (MQ=255)
                                                                                                tggCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATcg                                        >  2:743083/1‑140 (MQ=255)
                                                                                                tggCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATcg                                        >  1:554200/1‑140 (MQ=255)
                                                                                                tggCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATcg                                        >  1:1276653/1‑140 (MQ=255)
                                                                                                tggCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATcg                                        >  1:57332/1‑140 (MQ=255)
                                                                                                tggCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATcg                                        >  2:64588/1‑140 (MQ=255)
                                                                                                tggCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATcg                                        >  2:1085377/1‑140 (MQ=255)
                                                                                                tggCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATcg                                        >  2:1069612/1‑140 (MQ=255)
                                                                                                 ggCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATcgc                                       >  2:668750/1‑140 (MQ=255)
                                                                                                 ggCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATcgc                                       >  2:1099316/1‑140 (MQ=255)
                                                                                                  gCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATcgcg                                      >  1:1179090/1‑140 (MQ=255)
                                                                                                   cccccAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATCGCGt                                     >  2:369847/1‑140 (MQ=255)
                                                                                                   cccccAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATCGCGt                                     <  1:379877/140‑1 (MQ=255)
                                                                                                      ccAAAGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATCGCGTTCg                                  <  2:1233725/140‑1 (MQ=255)
                                                                                                        aaaGCCCCACTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATCGCGTTCGCa                                <  2:1441607/140‑1 (MQ=255)
                                                                                                                aCTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATCGCGTTCGCa                                >  1:76392/1‑132 (MQ=255)
                                                                                                                aCTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATCGCGTTCGCa                                <  2:76392/132‑1 (MQ=255)
                                                                                                                aCTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATCGCGTTCGCAGAgg                            >  2:546839/1‑136 (MQ=255)
                                                                                                                aCTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATCGCGTTCGCAGAgg                            <  1:546839/136‑1 (MQ=255)
                                                                                                                aCTGATACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATCGCGTTCGCAGAGGAAGa                        >  1:754818/1‑140 (MQ=255)
                                                                                                                    aTACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATCGCGTTCGCAGAGGAAGACGcc                    <  2:829251/140‑1 (MQ=255)
                                                                                                                    aTACAGGCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATCGCGTTCGCAGAGGAAGACGcc                    >  1:1061108/1‑140 (MQ=255)
                                                                                                                      aCAGGCGATAGCAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATCGCGTTCGCAGAGGAAGACGCCGa                  >  1:1320226/1‑140 (MQ=255)
                                                                                                                         ggCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATCGCGTTCGCAGAGGAAGACGCCGAgcg               <  1:277437/140‑1 (MQ=255)
                                                                                                                          gCGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATCGCGTTCGCAGAGGAAGACGCCGAgcgc              >  2:1278543/1‑140 (MQ=255)
                                                                                                                           cGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATCGCGTTCGCAGAGGAAGACGCCGAGcgcc             >  2:294145/1‑140 (MQ=255)
                                                                                                                           cGATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATCGCGTTCGCAGAGGAAGACGCCGAGcgcc             >  2:712862/1‑140 (MQ=255)
                                                                                                                            gATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATCGCGTTCGCAGAGGAAGACGCCGAGcgccg            >  2:1003380/1‑140 (MQ=255)
                                                                                                                            gATAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATCGCGTTCGCAGAGGAAGACGCCGAGcgccg            >  1:208603/1‑140 (MQ=255)
                                                                                                                              tAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATCGCGTTCGCAGAGGAAGACGCCGAGCgccgcc          >  2:1024505/1‑140 (MQ=255)
                                                                                                                              tAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATCGCGTTCGCAGAGGAAGACGCCGAGCgccgcc          >  2:115249/1‑140 (MQ=255)
                                                                                                                              tAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATCGCGTTCGCAGAGGAAGACGCCGAGCgccgcc          >  2:1207323/1‑140 (MQ=255)
                                                                                                                              tAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATCGCGTTCGCAGAGGAAGACGCCGAGCgccgcc          >  1:1340270/1‑140 (MQ=255)
                                                                                                                              tAACAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATCGCGTTCGCAGAGGAAGACGCCGAGCgccgcc          >  1:1090163/1‑140 (MQ=255)
                                                                                                                               aaCAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATCGCGTTCGCAGAGGAAGACGCCGAGCGCCGccc         >  1:709900/1‑140 (MQ=255)
                                                                                                                               aaCAAAGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATCGCGTTCGCAGAGGAAGACGCCGAGCGCCGccc         >  1:293289/1‑140 (MQ=255)
                                                                                                                                   aaGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATCGCGTCCGCAGAGGAAGACGCCGAGCGCCGCCCCGAc     <  2:374665/140‑1 (MQ=255)
                                                                                                                                    aGGGGCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATCGCGTTCGCAGAGGAAGACGCCGAGCGCCGCCCCGAcc    <  2:1061108/140‑1 (MQ=255)
                                                                                                                                      gggCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATCGCGTTCGCAGAGGAAGACGCCGAGCGCCGCCCCGACCTg  >  2:577014/1‑140 (MQ=255)
                                                                                                                                      gggCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATCGCGTTCGCAGAGGAAGACGCCGAGCGCCGCCCCGACCTg  <  1:668750/140‑1 (MQ=255)
                                                                                                                                      gggCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATCGCGTTCGCAGAGGAAGACGCCGAGCGCCGCCCCGACCTg  <  2:1090163/140‑1 (MQ=255)
                                                                                                                                      gggCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATCGCGTTCGCAGAGGAAGACGCCGAGCGCCGCCCCGACCTg  <  2:293289/140‑1 (MQ=255)
                                                                                                                                      gggCACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGACACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATCGCGTTCGCAGAGGAAGACGCCGAGCGCCGCCCCGACCTg  <  1:488112/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                        
TGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAACGCCGCTGATCCCGCCACCACTTTTTTCTGCGCATCGCGTTCGCAGAGGAAGACGCCGAGCGCCGCCCCGACCTG  >  NC_000913/3904319‑3904592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A