Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F28 I1 R1 183 98.6 3427438 96.9% 3321187 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,905,015 T→C K195E (AAA→GAA)  bglF ← beta‑glucoside specific PTS enzyme II/BglG kinase/BglG phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,905,0150TC100.0% 329.9 / NA 88K195E (AAA→GAA) bglFbeta‑glucoside specific PTS enzyme II/BglG kinase/BglG phosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/50);  total (38/50)

GGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTTTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTACCGCCGAAGCGTTTCCCCGCGGTGTATCCCAGGATTATCGGGAAGAACCAAAACAAGGC  >  NC_000913/3904882‑3905150
                                                                                                                                     |                                                                                                                                       
ggTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGccc                                                                                                                                   <  1:456746/140‑1 (MQ=255)
 gTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCg                                                                                                                                  <  2:782315/140‑1 (MQ=255)
         gCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGaa                                                                                                                          >  2:1544110/1‑140 (MQ=255)
          cATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGaaa                                                                                                                         >  1:1295320/1‑140 (MQ=255)
           aTTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAg                                                                                                                        <  2:201408/140‑1 (MQ=255)
            ttAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGc                                                                                                                       >  1:687464/1‑140 (MQ=255)
               aGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGt                                                                                                                    <  2:40338/140‑1 (MQ=255)
               aGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGt                                                                                                                    <  1:973504/140‑1 (MQ=255)
               aGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGt                                                                                                                    <  2:77598/140‑1 (MQ=255)
               aGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGt                                                                                                                    <  2:110669/140‑1 (MQ=255)
                gTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTc                                                                                                                   <  1:770274/140‑1 (MQ=255)
                 tCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCa                                                                                                                  <  2:687464/140‑1 (MQ=255)
                 tCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCa                                                                                                                  <  1:1149638/140‑1 (MQ=255)
                     cGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGt                                                                                                                    >  2:1647609/1‑134 (MQ=255)
                     cGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGt                                                                                                                    <  1:1647609/134‑1 (MQ=255)
                     cGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAAt                                                                                                              >  2:1204316/1‑140 (MQ=255)
                     cGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAAt                                                                                                              >  2:1473870/1‑140 (MQ=255)
                       ttCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTa                                                                                                            <  2:509065/140‑1 (MQ=255)
                            gAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGa                                                                                                       <  1:1544110/140‑1 (MQ=255)
                            gAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGa                                                                                                       <  2:44868/140‑1 (MQ=255)
                               tgctgcACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGc                                                                                                    >  2:1687153/1‑140 (MQ=255)
                                 ctgcACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCAc                                                                                                  >  1:470947/1‑140 (MQ=255)
                                 ctgcACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCAc                                                                                                  <  2:751863/140‑1 (MQ=255)
                                    cacaACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTaa                                                                                               >  2:1660983/1‑140 (MQ=255)
                                    cacaACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTaa                                                                                               >  2:758817/1‑140 (MQ=255)
                                    cacaACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTaa                                                                                               >  2:106613/1‑140 (MQ=255)
                                        aCCAGGCAGAAAAAATAATGGGAATAACC‑ATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTc                                                                                          <  2:1157695/140‑1 (MQ=255)
                                         ccAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACt                                                                                                 >  1:1251882/1‑133 (MQ=255)
                                         ccAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACt                                                                                                 <  2:1251882/133‑1 (MQ=255)
                                         ccAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTc                                                                                          <  2:1468440/140‑1 (MQ=255)
                                         ccAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTc                                                                                          <  2:1050697/140‑1 (MQ=255)
                                            ggCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTccac                                                                                       >  1:473025/1‑140 (MQ=255)
                                            ggCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTc                                                                                          <  2:61071/137‑1 (MQ=255)
                                            ggCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTc                                                                                          >  1:61071/1‑137 (MQ=255)
                                            ggCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATCGATGCACTAACGCTccac                                                                                       >  1:1430967/1‑140 (MQ=255)
                                                 aaaaaaTAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATc                                                                                  >  1:1197062/1‑140 (MQ=255)
                                                    aaaTAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCAcc                                                                               <  1:1009470/140‑1 (MQ=255)
                                                        aatGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATgg                                                                           <  1:1687153/140‑1 (MQ=255)
                                                          tGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCa                                                                         <  1:758817/140‑1 (MQ=255)
                                                           gggAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATgg                                                                           <  1:853519/137‑1 (MQ=255)
                                                           gggAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATgg                                                                           >  2:853519/1‑137 (MQ=255)
                                                               aTAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTaaa                                                                    <  2:473025/140‑1 (MQ=255)
                                                                tAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAAt                                                                   <  1:381668/140‑1 (MQ=255)
                                                                      aTGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTa                                                             >  2:1341672/1‑140 (MQ=255)
                                                                      aTGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTa                                                             >  2:1628720/1‑140 (MQ=255)
                                                                            aGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTAccgccg                                                       >  1:1695111/1‑140 (MQ=255)
                                                                             gTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTa                                                             <  1:1399746/133‑1 (MQ=255)
                                                                             gTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTa                                                             >  2:1399746/1‑133 (MQ=255)
                                                                             gTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTACCGCCGa                                                      >  2:1682610/1‑140 (MQ=255)
                                                                             gTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTACCGCCGa                                                      >  1:1456589/1‑140 (MQ=255)
                                                                                aTTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTACCGCCGAAGc                                                   >  2:356521/1‑140 (MQ=255)
                                                                                 ttCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTACCGCCGAAGCg                                                  >  1:981373/1‑140 (MQ=255)
                                                                                      caaTGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTACCGCCGAAGCGTTTcc                                             >  2:283476/1‑140 (MQ=255)
                                                                                         tgtgACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTACCGCCGAAGCGTTTCCCcg                                          <  1:1309970/140‑1 (MQ=255)
                                                                                         tgtgACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTACCGCCGAAGCGTTTCCCcg                                          <  2:1600853/140‑1 (MQ=255)
                                                                                         tgtgACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTACCGCCGAAGCGTTTCCCcg                                          <  1:1628720/140‑1 (MQ=255)
                                                                                             aCCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTACCGCCGAAGCGTTTCCCCGCGgt                                      <  1:1364942/140‑1 (MQ=255)
                                                                                             aCCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTACCGCCGAAGCGTTTCCCCGCGgt                                      >  1:468812/1‑140 (MQ=255)
                                                                                              ccGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTACCGCCGAAGCGTTTCCCCGCGgtg                                     <  1:230305/140‑1 (MQ=255)
                                                                                              ccGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTACCGCCGAAGCGTTTCCCCGCGgtg                                     <  1:283476/140‑1 (MQ=255)
                                                                                              ccGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATCGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTACCGCCGAAGCGTTTCCCCGCGgtg                                     <  2:1430967/140‑1 (MQ=255)
                                                                                              ccGGAATAACCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTACCGCCGAAGCGTTTCCCCGCGgtg                                     >  2:879665/1‑140 (MQ=255)
                                                                                               cGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTACCGCCGAAGCGTTTCCCCGCGgtgt                                    <  2:1456589/140‑1 (MQ=255)
                                                                                               cGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTACCGCCGAAGCGTTTCCCCGCGgtgt                                    <  1:1341672/140‑1 (MQ=255)
                                                                                                 gAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTACCGCCGAAGCGTTTCCCCGCGGTGTAt                                  >  1:325881/1‑140 (MQ=255)
                                                                                                  aaTACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTACCGCCGAAGCGTTTCCCCGCGGTGTATc                                 <  2:1295320/140‑1 (MQ=255)
                                                                                                   aTACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTACCGCCGAAGCGTTTCCCCGCGGTGTATcc                                >  2:1168031/1‑140 (MQ=255)
                                                                                                   aTACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTACCGCCGAAGCGTTTCCCCGCGGTGTATcc                                >  2:681174/1‑140 (MQ=255)
                                                                                                   aTACCCAGGAAATCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTACCGCCGAAGCGTTTCCCCGCGGTGTATcc                                <  2:470947/140‑1 (MQ=255)
                                                                                                            aaaTCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTACCGCCGAAGCGTTTCCCCGCgg                                       <  2:1250137/124‑1 (MQ=255)
                                                                                                            aaaTCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTACCGCCGAAGCGTTTCCCCGCgg                                       >  1:1250137/1‑124 (MQ=255)
                                                                                                            aaaTCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTACCGCCGAAGCGTTTCCCCGCGGTGTATCCCAGGATTAt                       <  2:1500930/140‑1 (MQ=255)
                                                                                                              aTCCAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTACCGCCGAAGCGTTTCCCCGCGGTGTATCCCAGGATTATCg                     >  1:701881/1‑140 (MQ=255)
                                                                                                                 cAGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTACCGCCGAAGCGTTTCCCCGCGGTGTATCCCAGGATTATCGGga                  <  1:317805/140‑1 (MQ=255)
                                                                                                                  aGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTACCGCCGAAGCGTTTCCCCGCGGTGTATCCCAGGATTATCGGgaa                 <  2:1219189/140‑1 (MQ=255)
                                                                                                                  aGCCCCAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTACCGCCGAAGCGTTTCCCCGCGGTGTATCCCAGGATTATCGGgaa                 <  2:1197062/140‑1 (MQ=255)
                                                                                                                     cccAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTACCGCCGAAGCGTTTCCCCGCGGTGTATCCCAGGATTATCGGgaagaa              >  1:1295149/1‑140 (MQ=255)
                                                                                                                      ccAGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCCCTAACGCTCCACCAATCACCATGGCAGTAAATGGATTACCGCCGAAGCGTTTCCCCGCGGTGTATCCCAGGATTATCGGGAAGAAc             <  1:80066/140‑1 (MQ=255)
                                                                                                                        aGCGCATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTACCGCCGAAGCGTTTCCCCGCGGTGTATCCCAGGATTATCGGGAAGAACCa           >  1:445056/1‑140 (MQ=255)
                                                                                                                         gcgcATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTACCGCCGAAGCGTTTCCCCGCGGTGTATCCCAGGATTATCGGGAAGAACCaa          <  2:1295149/140‑1 (MQ=255)
                                                                                                                         gcgcATCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTACCGCCGAAGCGTTTCCCCGCGGTGTATCCCAGGATTATCGGGAAGAACCaa          >  1:109948/1‑140 (MQ=255)
                                                                                                                           gcATCCGCTTCTTGCCCTTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTACCGCCGAAGCGTTTCCCCGCGGTGTATCCCAGGATTATCGGGAAGAACCaaaa        <  1:877549/140‑1 (MQ=255)
                                                                                                                             aTCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTACCGCCGAAGCGTTTCCCCGCGGTGTATCCCAGGATTATCGGga                  >  1:1368348/1‑128 (MQ=255)
                                                                                                                             aTCCGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTACCGCCGAAGCGTTTCCCCGCGGTGTATCCCAGGATTATCGGga                  <  2:1368348/128‑1 (MQ=255)
                                                                                                                               ccGCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTACCGCCGAAGCGTTTCCCCGCGGTGTATCCCAGGATTATCGGGAAGAACCAAAACAAg    >  1:222667/1‑140 (MQ=255)
                                                                                                                                 gCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCCCCATGGCAGTAAATGGATTACCGCCGAAGCGTTTCCCCGCGGTGTATCCCAGGATTATCGGGAAGAACCAAAACAAGGc  <  2:445056/140‑1 (MQ=255)
                                                                                                                                 gCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTACCGCCGAAGCGTTTCCCCGCGGTGTATCCCAGGATTATCGGGAAGAACCAAAACAAGGc  <  1:356521/140‑1 (MQ=255)
                                                                                                                                 gCTTCTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTACCGCCGAAGCGTTTCCCCGCGGTGTATCCCAGGATTATCGGGAAGAACCAAAACAAGGc  <  1:186667/140‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                       
GGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTTTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTACCGCCGAAGCGTTTCCCCGCGGTGTATCCCAGGATTATCGGGAAGAACCAAAACAAGGC  >  NC_000913/3904882‑3905150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A